JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

Course Structure and Syllabus for M. Pharm- Pharmaceutical Analysis

for affiliated and constituent Pharmacy Colleges 2009-10
I YEAR

I SEMESTER
Subject

Modern Pharmaceutical Analytical Techniques

Biostatistics, Intellectual property rights & regulatory
affairs
Quality control and validation
Analytical methodology
Modern Pharmaceutical Analysis-Practical
Analytical methodology Practical
Mini-project- I
I YEAR

Chemical and biological evaluation

Advanced pharmaceutical analysis
Drug analysis
Formulation and cosmetic analysis
Advanced pharmaceutical analysis - Practical
Formulation and cosmetic analysis - Practical
Mini-project- II

(III & IV Semesters)

SUBJECTS

Seminar
Project work

4
4
6
6
3

II SEMESTER
Subject

II YEAR

Hours/
Week
4
4

Hours/
Week
4
4
4
4
6
6
3

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
MODERN PHARMACEUTICAL ANALYSIS
1. UV-VISIBLE SPECTROSCOPY: Brief review of electromagnetic spectrum, UVVisual range, energy, wavelength and color relationships. Interaction of
electromagnetic radiation (UV-visible) with matter and its effects. Chromophores and
their interactions with E.M.R. Absorption spectra of organic compounds and
complexes illustrating the phenomenon and its utilization in qualitative and
quantitative studies of drugs. Shifts and their interpretation (including solvent
effects). Empirical correlation of structure with absorption phenomena (Woodwards
rules etc) Quantitative estimations, Modern instrumentation.
2. a) INFRARED SPECTROSCOPY: Nature of Infra-red radiation. Interaction of I.R
radiation with I.R molecules and effects on bonds. Molecular Infrared Spectra.
Brief outline of classical I.R instrumentation and practical details of obtaining
spectra, including sample preparation for spectroscopy, quantitative interpretation
of I.R spectroscopy including FT-IR, ATR.
b) OPTICAL ROTATORY DISPERSION: Fundamental principles of ORD,
cotton effect curves, their characteristics and interpretation. Octant rule and its
application with examples. Circular dichroism and its relation to ORD.
3. NMR SPECTROSCOPY: Fundamental principles of NMR (Magnetic properties of
nuclei, applied field and precession; absorption and transition; frequency). Chemical
shifts concept: Isotopic nuclei, Reference standards: Proton magnetic spectra, their
characteristics, presentation terms used in describing spect5ra and their interpretation
(Signal No., Position, Intensity). Brief outline of instrumental arrangements and some
practical details. Signal multiplicity phenomenon in high resolution PMR. Spin-spin
coupling. Application of Signal split and coupling constant data to interpretation of
spectra. De-coupling and shift reagent methods. Brief outline of principles of FTNMR with reference to 13CNMR. Spin-spin and spin-lattice relaxation phenomenon.
Free induction decay (FID) proton noise de-coupling signal, average time domain and
frequency domain signals nuclear overhauser enhancement 13CNMR spectra, their
presentation; characteristics, interpretation, examples and applications. Brief
indication of application of magnetic resonance spectral data of other nuclei by
modern NMR instruments. Introduction to 2-D NMR techniques.
4. MASS SPECTROSCOPY: Basic principles and brief outline of instrumentation. Ion
formation and types; molecular ion, Meta stable ions, fragmentation processes.
Fragmentation patterns and fragmentation characteristics in relation to parent
structure and functional groups. Relative abundances of isotopes and their
contribution to characteristic peaks. Mass spectrum, its characteristics, presentation
and interpretation. Chemical ionization Mass Spectroscopy. GC-MS, other recent
advances in MS. Fast atom bombardment mass spectrometry.

5. CHROMATOGRAPHIC TECHNIQUES: Classification of chromatographic

methods based on mechanism of separation. Paper chromatography; techniques and
applications. Thin Layer Chromatography, comparison to paper chromatography and
HPLC, adsorbents for TLC. Preparation techniques, mobile phase selection, reversed
phase TLC, High performance TLC detection methods, quantitative methods in TLC.
Programmed multiple development techniques.
6. GAS CHROMATOGRAPHY: Instrumentation packed and open tubular column,
Column efficiency parameters, the Vandeemeter equation, Resolution, liquid
stationary phase, derivitazation methods of GC including acylation, perfloro
acylation, alkylation and esterification. Detectors: FID, ECD, TCD, NPDA. Critical
comparison of sensitivity, selectivity and field of applications of these detectors.
Examples of GC applications in pharmaceutical analysis.
7. LIQUID CHROMATOGRAPHY: Comparison of GC and HPLC, instrumentation
in HPLC, analytical, preparative and microbore columns, normal and reversed phase
packing materials, reverse phase HPLC, Column selection, Mobile phase selection,
Efficiency parameters, resolution, detectors in HPLC refractive index, photometric
and electrochemical. Comparison of sensitivity, selectivity and field of applications of
these detectors. HPTLC-instrumentation and applications.
8. ELECTROPHORESIS: Moving boundary electrophoresis, Zone electrophoresis,
Isotacophoresis and applications in pharmacy.
X-ray Diffraction methods: introduction, generation of X-rays, elementary
crystallography, Miller Indices, X-rays diffraction, Braggs law, X-ray powder
diffraction, X-ray powder diffractometer, obtaining and interpretation of X-ray
powder diffraction data. Principle, instrumentation and application of the following:
Differential Scanning Colorimetry (DSC), DTA &TGA in analysis of
pharmaceuticals.
REFERENCES:
1. Instrumental methods of chemical analysis by chatwal. K, anand, 5 th edition.
2. Vogels text book of quantitative chemical analysis by G.H.Jeffery, J.Bassett,
J.Mendhan, R.C.Denny.
3. Instrumental methods of analysis by Willard, Merit, Dean, Settle.
4. Organnic spectroscopy by Y.R.Sharma.
5. Spectrometric identification of organic compounds by silverstein, Webster.
6. Spectroscopy by B.K.Sharma
7. Fundamentals of analytical chemistry by Skoog
8. Instrumental methods of analysis by Skoog.
9. Text book of pharmaceutical analysis by S.Ravishankar.
10. Organic spectroscopy by William kemp
11. Spectroscopic methods in Organic chemistry by Dudley William and Ian
Flemming, Tata Mc Graw Hill.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
BIO-STATISTICS, INTELLECTUAL PROPERTY RIGHTS &
REGULATORY AFFAIRS
I.

BIO-STATISTICS
1. An introduction to statistics and biostatistics-collection and organization of data,
graphical, pictorial presentation of data, measures of central tendency and
dispersion, sampling techniques, sample size, Coefficient of variation, mean error,
relative error, precision and accuracy
2. Tests of significance: Testing hypotheses Principles and applications of Z, t, F
ratio and chi-square tests in pharmaceutical and medical research. Non-parametric
tests: sign test, Wilcoxon signed rank test, Wilcoxon rank sum test, Kruskal
Wallis test, run test and median tests.
3. Design of Experiments: Principles of randomization, replication and local
control; CRD, RBD, LSD their applications and analysis of data; Factorial
Experiments Principles and applications; Probit analysis: Dose effect
relationships, calculation of LD50, ED50.
4. Statistical quality control: Meaning and uses, Construction of

X, R, P, p and

charts.
II.

INTELLECTUAL PROPERTY RIGHTS & REGULATORY AFFAIRS

1. Patents and Intellectual Property Rights (IPR): Definition, scope, objectives,

sources of patent information, patent processing and application. Patents,
Copyrights, Trademarks, Salient features, international and regional agreements.

2. GATT & WTO: GATT Historical perspective, objectives, fundamental

principles, impact on developing countries. WTO objectives, scope, functions,

structure, status, membership and withdrawal, dispute settlement, impact on
globalization, India task and challenges, trade related aspects (TRIPS).

3. Regulatory Affairs : Indian context requirements and guidelines of GMP,

understanding of Drugs and Cosmetics Act 1940 and Rules 1945 with reference to
Schedule N ,U & Y.

4. a).Related Quality Systems: Objectives and guidelines of USFDA, WHO and

ICH. Introduction to ISO series.

b).Documentation: Types related to pharmaceutical industry, protocols,

harmonizing formulations, development for global filings, ANDA, NDA, CTD,
dealing with post approval changes SUPAC, handling and maintenance
including electronic documentation.

REFERENCES:
1.
2.
3.
4.
5.
6.

KS Negi Biostatistics AITB Publishers, Delhi.

Irfan Alikhan Fundamentals of Biostatistics Ukaaz Publications
Khan and Khanum Biostatistics for Pharmacy Ukaaz Publications
J.E, Demuth Basic statistics and Pharmaceutical applications Mercel & Dekker.
Applied statistics by S.C.Gupta & V.K.Kapoor
Funadamentals of mathematical statistics by S.C.Gupta & V.K.Kapoor

7. Good Manufacturing Practices for Pharmaceuticals, S.H. Wiling, Vol. 78, Marcel
Decker.
8. Protection of Industrial Property rights, P. Das & Gokul Das
9. Law and Drugs, Law Publications. S.N. Katju
10. Original Laws Published By Govt. of India
11. Laws of drugs in India, Hussain
12. New Drug Approval Process, R.A.Guarino,Vol 100, Marcel Decker, NY
13. fda.org,wipo.int,patentlawlinks.com, hc-sc.gc.ca,ich.org,cder.org
Dekkers series

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
QUALITY CONTROL AND VALIDATION

1. Quality control, layout, responsibilities, good laboratory practice, training,

Calibration of instruments, sampling techniques, Specifications, SOPs.
Documentation review and batch release. Vendor and batch release. Vender and
where house audit. Working references and pharmacopeial standards.
2. Retention of active pharmaceutical ingredients and finished formulations and
quality review. Schedule M of drug rules and WHO certification for export of
pharmaceuticals. Salving of returned good and reprocessing.
3. Application of computers in quality control laboratory.
4. Validation: Method validation, Cleaning Validation, Personal validation.
5. Development of drug information profiles.
6. Enzyme immunoassay. Concepts and Methodology.
7. Concept of QA requirements of CGMP, GLP, ISO 9000 Series.
8. Analysis of industrial samples: lead in paint Hg container pharmaceuticals, heavy
metals in fertilizers, determination of Pb in gasoline and steel air, copper
compound in human serum.

References:
1. Pharmacopoeias

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
ANALYTICAL METHODOLOGY
1.

Application of instrumental methods in the development and use of medicines:

Introduction, Product characterization for drug development, product
development, production and pharmacopoeial controls, concept of analytical
method development.

2.

Analysis of drugs and excipients in the solid state introduction, Particle size
analysis, importance of particle size in various dosage forms, methods of practical
size analysis. X-ray powder diffraction

3.

light scattering methods in quantitative analysis

a. Turbidometry
b. Nephlometry.

4.

Light emission methods in quantitative analysis

a. Flourimetry
b. Flame photometry.

5.

Analytical method validation parameters: Spectrophotometric, HPLC and GC

methods statistical analysis and significance in analytical methods.

Quality control of radiopharmaceutical and radiochemical methods in analysis.

7.

Preparation of drug samples for analysis: Pharmaceutical samples, fundamental

theories, controlling preparation techniques, specific sample techniques.

8.

Precipitation titration: Titration curves, feasibility of precipitation titration, factors

affecting shape titrant and analyte concentration, selection and evaluation of
external and adsorption indicators of end points. Quantitative application of
precipitation titrations.

REFERENCES:
1. Instrumental methods of chemical analysis by chatwal. K, anand, 5 th edition.
2. Vogels text book of quantitative chemical analysis by G.H.Jeffery, J.Bassett,
J.Mendhan, R.C.Denny.
3. Instrumental methods of analysis by Willard, Merit, Dean, Settle.
4. Organnic spectroscopy by Y.R.Sharma.
5. Spectrometric identification of organic compounds by silverstein, Webster.
6. Spectroscopy by B.K.Sharma
7. Fundamentals of analytical chemistry by Skoog
8. instrumental methods of analysis by Skoog.
9. Text book of pharmaceutical analysis by S.Ravishankar.
10. TLC by Wagner

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
MODERN PHARMACEUTICAL ANALYSIS - PRACTICALS

1.

Simultaneous estimation of Paracetamol Ibuprofen, Rifampicin and INH,

aspirin and caffeine.

2.

UV-Visible spectrum scanning of certain organic compounds- absorption and corelation of structures, comparisions.
a. Chloromphenicol
b. Sulphadiazine
c. Analgin

3.

Effect of pH and solvent and UV spectrum of certain drugs.

4.

Two dimensional paper chromatography and TLC.

5.

Gradient elution and other techniques in column chromatography.

6.

Separation by electrophoresis.

7.

Experiments based on HPLC and GC.

8.

IR, NMR and Mass spectroscopy on compound each.

9.

DSC/XRD curves of a sample and mixture to understand polymorphism.

10.

Determination of insulin / any other hormones by ELISA method.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year I semester
ANALYTICAL METHODOLOGY - PRACTICALS

1.

Determination of chloride and sulfate in calcium gluconate by

nephloturbidometric analysis

2.

Estimation of following drugs by fluorimetry

a. Doxazosin mesylate.
b. Riboflavin
c. Thiamine.
d. Terazocin.

3.

Study of the quenching in fluorimetry- eg. quenching of quinine fluorescence by

iodide ions.

4.

Determination of sodium/ potassium by flame photometry.

5.

Colorimetric estimation of sulphadiazine/sulphaacetamide using

N-(1- naphthyl) ethylenediamine di HCl.

6.

Quantitative determination of following groups.

a. Hydroxyl b. carboxyl c. Methoxyl
d. Amine
f. Esters.
of

e. Aldehyde

7.

Quantitative colorimetric determination

paradimethylaminocinamaldehyde reagent.

any

drug

by

using

8.

Quantitative colorimetric determination of any drug by using MBTH reagent.

9.

Colorimetric estimations of ferrous ions using 1, 10-phenanthroline.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
CHEMICAL AND BIOLOGIACL EVALUATION THEORY

1.
2
3.

Sterility testing methodology and interpretation

Tests for effectiveness of antimicrobial preservatives.
Detailed study of principles and procedures involved in the biological assay of the
following:
a). Adsorbed Diphtheria vaccine.
b). absorbed Tetanus vaccine.
c).Chorionic Gonandrotrophin.
d). Diphtheria antitoxin
e). Gas Gangrene antitoxin.
f). Heparin sodium.
g). Human anti hemophilic fraction.
h).Japanese encephalitis vaccine
I) Oxytocin
j). Pertussis vaccine.
k) Plague vaccine.
l) Rabies vaccine.
n).Streptokinase.
o).Tetanus Antitoxin
p).tuberculin purified protein derivative.
q). typhus vaccine/
r) Urokinase.
s).Vasopressin activity.

4.

Pyrogens-Production, chemistry and properties of bacterial Pyrogens and

endotoxins. Pyrogens testion: IP,BP and USP methods. Interpretation of data
comparison with other official pyrogen tests.

5.

Microbial assay of Vitamins and antibiotics.

6.

Chemical and bacteriological analysis of portable water, Purified water and water
for injection.

7.

Clinical analysis: clinical analysis of the composition of blood collection and

preservation of the sample, clinical analysis of blood glucose, blood urea nitrogen,
immuno assay, the blood gas analyzer, trace elements in the body.

8.

Forensic analysis: general discussion of poisons, organophosphates and snake

venom, estimation of poisonous materials such as lead, mercury and barbiturates
in biological materials.

REFERENCES:
1.
2.
3.
4.
5.

A guide to quality management by Koushik Maithra & Shadan.K.Gosh.

How to practice GMP by P.P.Sharma.
QA manual by D.H.Shah
Basic test for pharmaceutical substances WHO 1988.
Elements in biotechnology by P. K. Gupta.

6. Molecular biology and biotechnology by J. M Walker and E. D. Gingold.

7. Pharmaceutical biotechnology by S. P. Vyas and V. K. Dixit.
8. Biotechnological applications to tissue culture by Shargool.
9. Immunology, An Introduction- Tizard, 4th Edn. Saunders College Publication,
1995
10. Biotechnology. Principles and Application, I.J. Higgins, D.J. Best, J. Jones,
Blackwell Scientific Publications, Oxford, London 1988.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
ADVANCED PHARMACEUTICAL ANALYSIS
1.

A detailed study of principles and procedures involved in various physicochemical methods of analysis including instrumental methods of analysis of
pharmaceutical dosage forms containing the following classes of drugs.
a. Sulphonamides.
b. Barbiturates.
c. Adrenergic drugs.
d. Anti tubercular drugs.
e. Diuretics.
f. Anti-malarials
g. Local anesthetics.
h. General anesthetics.
i. Analgesics and anti pyretics.
j. Antihelmentics.

2.

Principles and procedures involved in the analysis of pharmaceutical preparations

and dosage forms containing the following groups of substances.
a. Alkaloids
b. Glycosides
c. Vitamins.
d. Antibiotics.
e. Steroid hormones

3.

a) Elementary analysis non-metals and metals

b) Principles and procedures involved in the quantitative determination of
following groups
a. Hydroxyl
b. Carboxylic acid.
c. Aldehydes
d. Ketones.
e. Methoxyl
f. Esters
g. Amines
h. Nitrates.

4.

Principles and procedures involver in the use of the following reagents in

pharmaceutical analysis.
a.
b.
c.
d.

MBTH (3-methyl-2-benzothiazolone hydrazone) reagent.

FC reagent
2,6 - dichloroquinine monoamine reagent.
1,2 napthaquinone-4-sulfonate reagent

e. 2, 3, 5-tri phenyl tetrazonium salt.

f. PDAB (paramethyle aminobenzaldehyde) reagent
g. PDACA (paradimethyleamino cinnamaldehyde) reagent
h. Ninhydrine Reagent
i. Carr-Price reagent
j. Baratton-Marshal reagent.
k. 2, 6-dicholroquinone chloride
5.

Assay of official compounds by HPLC, GC, and IP1996

6.

Interpretation of spectral data of Infrared spectroscopy, H 1 N.M.R & C13 N.M.R

and Mass spectroscopy for structural elucidation of organic molecules.

7.

A detaile study of principles, instrumentation and application in drug analysis of:

GC-MS, LC-MS with drug metabolism, toxicological and forensic studies,
diagnosis of disease state, quantification of dugs in biological samples, super
critical fluid chromatography and size exclusion chromatography.

8.

Brief study of the theory, instrumentation and application of the following

analytical techniques: Atomic force microscopy, plasma atomic emission
spectroscopy, photon correlation spectroscopy.

REFERENCE:
1. A.I.Vogel text book of inorganic chemistry , 4 th edition, ELBS
publication, London
2. Becket and Stanlake pharmaceutical chemistry, 3rd edition, Vol-I
& II, CBS publishers, New Delhi.
3. K.A.Connors text book of pharmaceutical analysis, 3 rd edition, Willey
Interscience publication Newyork.
4. Instrumental methods of analysis by Willard, Merit, Dean, Settle.
5. Instrumental methods of analysis by Skoog.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
DRUGS ANALYSIS
1.

Classification of reactions in Titrimetric analysis, standard solutions, preparations

of standard solutions, primary and secondary standard substances. Theory of acid
base titration. Neutralization indicators, mixed and universal indicators,
neutralization curve and displacement titrations.

2.

Non aqueous titrations involving the following.

a. Primary, secondary and tertiary amines.
b. Halogenated salts of bases.
c. Acidic substances.
d. assay of official drugs in IP1996 by non aqueous titrimetry.
C). Aquametry: determination of water by titration with Karl Fischer Reagents
(KFR)

3.

Principles and pharmaceutical applications of complexometric titrations

involving:
a. Potassium Iodate/Bromate titrations
b. Ceric ammonium sulphate titrations
c. Titanus chloride titration

4.

Principles and pharmaceutical applications of complex-metric titrations

involving:
a. Direct titration of polymetalic system with sodium editate.
b. Back titration with sodium editate.
c. Titration involving the displacement of one complex by another.
d. PM indicators

5.

a).Principles and procedures involved in gravimetric and argentimetric analysis

with examples official in IP1996.
b). Diazotization titration.

6.

Thermo - analytical methods of analysis

a. Thermo gravimetric analysis
b. Differential thermal analysis.

7.

Quality control of crude drugs- ash value, extraction value, fiber content, powder
analysis quantitative microscopy and micro chemical tests.

8.

Techniques in the estimation of enzyme and the endogenious substances in the

body fluids in the physiological and pathological condition.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
FORMULATION AND COSMETICAL ANALYSIS
1.

2.

3.

Principles and pharmaceutical applications of electro-analytical methods-I.

i. Potentiometry
ii. pH measurements.
iii. Polarography
Principles and pharmaceutical applications of electro-analytical methods-II
i. Amperometry
ii. High frequency titrations
iii. Conductometric titrations.
Identifications and quantitative determination of preservatives, antioxidants,
coloring materials, emulsifiers and stabilizers in pharmaceutical formulations.

4.

Quality control of tablets, capsules, liquid dosage forms, parental preparations,

ointments and creams suppositories and controlled release products.

5.

Quality control of containers, closures, caps and secondary packing materials like
paper and board for pharmaceuticals.

a). Quality control of cosmetic products- Hair care products, skin care products,
color cosmetics, baby care products, ethnic products, dental products, personal
hygiene products, color makeup preparations, lipsticks, hair setting locations
and eye shadows.
b). Toxicity testing in cosmetic industry, Safety and legislation in cosmetic
industry

7.

a) Various types of raw materials used in the cosmetic industry for the
manufacture of finished products.
b) Methods of analysis to determine the quality of raw materials used in
cosmetic industry.

8.

The study of sources and description of herbal origin used like fixed oils, gums,
hydrophilic colloids, clours, perfumes, protective agents, preservatives, anti
oxidants and ancillary agents.

REFERENCES:
1. W.A.Poucher: Pouchers perfumes, Cosmetics and soaps. Vol.3,9 th edition,
Chapman and Hill , London
2. J.B.Wilkinson and R.J.Moore: Herrys Cosmeticology, Longman Scientific and
Technical Publishers,SIngaore.
3. The controller of publications; New Delhi, Govt.of India Pharmacopeia, Vol.I and
II,1996

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
ADVANCED PHARMACEUTICAL ANALYSIS - PRACTICALS
1.
2.

Assay of reserpine injection, IP1996

Quantitative analysis of drugs in the following multicomponent dosage forms.
a. Ibuprofen, paracetamol and chlorzoxazone.
b. Amoxicillin and probenecid
c. Diazepam and diphenylhydramine HCL
d. Clotrimazole and tinidazole
e. Cloxacillin and ampicillin

3.

Assay of paracetamol tablets, IP1996

4.

Assay of phenylephrine injection IP1996

5.

Assay of Atropine sulphate tablets, IP1996

Assay of Benzhexol hydrochloride tablets.

7.

Identification and verification of standards for a sample of caster oil, IP1996

8.

Identification and verification of standards for a sample cetyl alcohol, IP1996

9.

Validation of analytical instruments.

10

Validation of pharmaceutical manufacturing machinery

11.

Validation of pharmaceutical manufacturing process.

12.

Validation of analytical methods

13

Standard operating procedures- for analytical instrumentation.

14

Standard operating procedures- cleaning process.

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY ANANTAPUR

M.Pharm Pharmaceutical Analysis
I year II semester
FORMULATION AND COSMETICAL ANALYSIS - PRACTICAL
1.

Assay of bisacodyl suppositories, IP1996

2.

Assay of Sodium diatrizoate injection, IP1996

3.

Determination of moisture content in the following drugs using Karl-Fischer

Reagent.
a.
Ampicillin trihydrate.
b.
Fructose
c.
Gentamycin sulphate
d.
Calcium lactate or gluconate / emetin dihydrochloride

4.

Assay of cephalexin capsule, IP1996

5.

Assay of calcium gluconate injection, IP1996

6.

Assay of sodium aurothiomalate injection, IP1996

7.

Quality control test for Ointments and creams.

8.

Quality control test for pessaries, suppositories and controlled release products.

9.

Detection and Quantitative determination of preservatives.

10.

Detection and Quantitative determination of antioxidants.

11.

Quality control tests for pharmaceutical containers.

12.

Quality control testing of pharmaceutical containers.

13.

Quality control of some cosmetic preparations.

Mini Projects:
The mini projects can be taken up as industrial visit/training and report submission.
Or
A suitable project shall be carried out in the college.
The Project Work:
Separate guidelines will be issued