Methionine PDF
Methionine PDF
Methionine PDF
org
Because synthetic amino acids are not allowed in organic livestock production, and animal slaughter
by-products cannot be used in organic feed, nutrition can present challenges in organic poultry production. Providing the amino acid methionine (MET) without oversupplying protein is generally the
most difficult. Feed rations that are high in plant proteins, such as soybean meal, can be used instead of
synthetic MET, but high-protein diets are not healthy for poultry or the environment. Diets containing
fishmeal, milk products, and nonconventional sources of protein, such as earthworms or insects, can
help provide MET, but the ingredients are expensive and, in most cases, not available in organic form.
It is difficult to design diets with sufficient MET and balance of other essential amino acids.
Contents
Introduction ......................1
Synthetic
Methionine ........................2
Natural
Methionine
Supplement.......................3
Methionine
Requirements
of Poultry ............................3
Methionine
Deficiency
Problems ............................5
Methionine
in Feedstuffs......................6
Formulating
Diets and
Feeding
Strategies ...........................9
Conclusions .................... 10
References .......................11
Appendices .............. 13-17
ATTRA (www.attra.ncat.org)
is a program of the National
Center for Appropriate Technology
(NCAT). The program is funded
through a cooperative agreement
with the United States Department
of Agricultures Rural BusinessCooperative Service. Visit the
NCAT website (www.ncat.org)
for more information on
our other sustainable
agriculture and
energy projects.
www.attra.ncat.org
Introduction
maximizing production (weight gain, feed efficiency, etc.). However, economic returns are a
concern for any commercial operation. For more
information on organic poultry requirements, see
ATTRAsOrganic Poultry Production.
In the United States, synthetic methionine
(MET) has been the only synthetic amino acid
allowed in organic livestock production, and only
in poultry. When the NOP organic rule was first
published in 2002, the MET allowance was to
end in 2005. An extension was granted until 2008
and then again until October 2010. Finally, in
2012, a final rule was published to allow synthetic
methionine to be added into feed at a level not to
exceed two pounds per ton for chickens (laying
hens and broilers) and three pounds for turkeys
and all other poultry.
Related ATTRA
Publications
www.attra.ncat.org
Arsenic in
Poultry Litter:
Organic Regulations
Organic Poultry
Production
Tipsheet: Organic
Poultry Production
for Meat and Eggs
Pastured Poultry
Nutrition and Forages
Synthetic Methionine
Synthetic MET is manufactured as a pure
amino acid. Common forms are DL-MET and
2-hydroxy-4 (methylthio) butanoic acid (a.k.a.
methionine hydroxyl analogue free acid). Raw
materials for DL-MET include oil, natural gas,
air, and water which are used to make propene,
sulphuric acid (H2S), methanol (CH3OH), and
ammonia (NH3) for manufacture of DL-MET
(Binder, 2003). DL-Methionine (the DL refers
to the racemic mixture), comes in a crystalline
form and is 99% available MET. It is available
from the companies Degussa and Adisseo. Methionine hydroxyl analogue comes in a liquid form
and is 88% available MET. Synthetic MET is so
pure that even in non-organic rations where no
restrictions apply to its use, only five pounds of
DL-MET are needed per U.S. ton of feed. However, in organic rations it is limited to two pounds
per ton in chicken feed, and three pounds per ton
in turkey and other poultry rations.
Products with zinc methionine available from
Zinpro are listed by the Organic Material Review
Institute (OMRI) as being permitted in organic livestock production. 4-Plex-E has 20% MET (directions for feeding poultry are 1.6 pounds per U.S.
ton). Zinpro-E has 40% MET (directions for feeding poultry are 0.4 pounds per U.S. ton). Because
feeding directions are intended to satisfy zinc levels,
only a small amount of MET is provided.
Page 2
Natural Methionine
Supplement
Possible methods for developing a natural MET
supplement include fermentation, extraction, or
protein hydrolysis.
Many amino acids are produced commercially by
bacterial fermentation. Because genetically modified organisms are not permitted in organic production, any MET-producing bacteria would need
to be naturally selected. However, high levels of
MET are toxic to bacteria, so the yields from
fermentation are very low and not cost-effective.
MET can be extracted from intact proteins or isolated from partially hydrolyzed proteins, but there
are no such products on the market for livestock.
Methionine Requirements
of Poultry
Poultry do not have specific requirements for
crude protein levels; only for amino acid levels.
Amino acid requirements are usually presented
as percentages of the diet. They may also be presented as a percentage of the protein requirement.
The National Research Councils (NRC) Nutrient Requirements of Poultryis commonly used in
the United States. The requirements of broilers
are given for starter, grower, and finisher phases
because the requirements change as the bird grows
(less amino acids and more energy are required
with age). Overall crude protein levels of 23, 20,
and 18% are used for starter, grower, and finisher
phases, respectively. The MET and CYS requirements are listed in Table 1.
The NRC lists the total amino acid requirement
rather than digestible amino acid. Baker (1997)
specifies digestible amino acid requirements for
broilers. In the starter phase, they are 0.41% for
MET and 0.41% for CYS.
Protein and amino acid requirements vary considerably according to the productive state of the
bird, that is, the rate of growth or egg production. For example, a mature rooster is bigger than
a hen; however, the laying hen has higher amino
acid requirements due to egg production.
Factors affecting responses of poultry to amino
acids include environmental temperature, dietary
factors, immunological stress, age, species, genetics, and gender. These factors either influence feed
intake or reduce the efficiency of use of an amino
acid (DMello, 1994). For example, male meat
birds need more essential amino acids and feed
than females.
Grower
Finisher
Methionine
0.50
0.38
0.32
Methionine + cystine
0.90
0.72
0.60
www.attra.ncat.org
Page 3
Methionine Requirements of
Slow-Growing Meat Chickens
Dietary energy exerts its effect through variations in feed intake (Emmert, no date). As dietary
energy levels increase, feed intake decreases. A
high-energy diet effectively limits feed intake,
which also limits protein and amino acid intake.
Therefore, a high dietary concentration of amino
acids is needed for high-energy diets. The NRC
nutrient requirements are based on high-energy,
high-protein (high amino acid level) diets.
In contrast, as energy decreases, feed intake
increases, requiring a lower concentration of
nutrients in the diet. If low concentrations of
amino acids are used, diets should be low-energy
so that feed intake will be increased.
Birds eat less when it is hot and more when it is
cold (NRC, 1994). Therefore, diets usually need
to be higher in amino acids during hot weather
in order to make sure birds get enough.
Feeding diets deficient in essential amino acids
can also increase feed intake. Cherry & Siegel
(1981) fed pullets diets that were equal in energy
and contained 15% crude protein and only differed in levels of MET and SAA. They found that
the pullets compensated for a marginal deficiency
of SAA by increasing their feed consumption,
and that the SAA requirement for maximum feed
conversion efficiency was greater than the requirement for egg production.
According to Larbier and Leclercq (1994), small
birds such as leghorns are able to keep energy
Page 4
Laying Hens
The requirements for layers are given on the basis
of feed intake. For example, 0.30% MET and
0.58% SAA are required for lightweight leghorn-type layers that consume 100 grams (0.22
pounds) of feed per day. Laying hens have lower
MET requirements than meat birds. During
weeks 16 to 22, layers are still growing and, at
the same time, laying eggs and will require elevated levels of methionine.
Turkeys
Turkey poults have very high amino acid requirements to meet the demands of their rapid growth.
It can be hard to get sufficient amino acids into
poults in the starter phase because feed intake is
low, and the poults need to accrete a lot of protein.
This is especially difficult to do without synthetic
MET. From zero to four weeks, 0.55% MET and
1.05% SAA are required. Amino acid needs of
turkeys differ substantially by gender.
ow-nutrient
diets or feed
restrictions
are often used in the
starter phase to
slow the growth of
fast-growing birds
in order to reduce
metabolic disorders
and lameness.
Methionine, (g/kg)
Page 5
Methionine in Feedstuffs
Methionine and cysteine are present as intact
proteins in various feedstuffs. (Methionine is
CYS
0.48
0.56
0.54
0.59
Fishmeal, menhaden
1.68
0.5
0.64
0.43
Casein
2.56
0.4
0.79
0.33
Rice
0.22
0.19
0.81
0.58
0.72
0.85
Potato protein
1.64
1.06
0.9
Algae
1.33
0.55
Rapeseed, full-fat
0.38
0.46
0.64
0.7
0.67
0.49
Sunflower, full-fat
0.38
0.3
Sesame meal
1.06
0.6
Safflower meal
0.38
0.41
Flax meal
0.35
0.42
0.51
0.48
Alfalfa meal
0.21
0.16
Grass
0.27
0.16
1.46
1.06
Corn
0.17
0.18
Wheat
0.19
0.27
Field peas
0.19
0.31
Whey powder
0.17
0.24
Notes
Sources: AminoDat Degussa Amino Acid database; Kratzer and Vorha, 1996
Page 6
Plant Protein
If only plant protein is used in feed rations, more
protein is required than when animal proteins
are also used. However, high-protein diets are
not good for the birds or the environment. Highprotein diets usually lead to excess nitrogen in
manure and can be hard on the birds digestive
system. Various types of plant protein are discussed below.
Oilseed meals, such as soybean meal, are
common poultry feeds after the oil has
been extracted for the vegetable oil market,
leaving a high-protein meal. However, the
extraction process uses chemical solvents,
and the remaining meal is not permitted in
organic production. Organic soybeans are
produced in full-fat (roasted or extruded)
or meal (expelled) form.
Many legumes and oil seeds such as field
beans, field peas, lentils, etc. have antinutritional factors (ANF), including tannins
and lectins. Some ANF can be removed by
processing or heat-treatment. For example,
soybeans have a trypsin inhibitor and must
be heat-treated to destroy it.
Sesame meal has a high MET content
(1.06%); however, the MET is not welldigested and is also low in lysine.
Sunflower meal has a MET content similar
to soybean meal. Chickens cannot remove
the hull of whole sunflower seeds. A SARE
www.attra.ncat.org
ighprotein
diets
usually lead to
excess nitrogen in
manure and can be
hard on the birds
digestive system.
Animal Protein
Animal protein is high-quality and a good source
of MET. In a pasture setting, poultry consume
many sources of animal protein, including insects
and worms. In the past, animal slaughter by-products (i.e., meat and bone meal) were important
ingredients in conventional poultry diets, but they
are banned in organic livestock feed production.
However, other animal products can be used,
such as fishmeal and milk products, and these are
discussed below. Insects and worms can provide
Page 7
arthworms
and insects
are highquality proteins,
similar to fishmeal,
and high in MET.
Additional Proteins
Outdoor access is required in organic poultry
production but pasture is not. Many operations
provide small areas (organic regulations do not
specify stocking density or area size for outdoor
areas); therefore, birds may not have significant
access to grassy areas. Pastured poultry producers, however, usually provide extensive outdoor
access by way of small portable houses moved
regularly to fresh pasture. Forage is a source of
MET. Although the MET level of forage is generally low to moderate, foraging should be encouraged. Birds can also obtain high-quality protein
from insects and worms on pasture.
Moritz et al. (2005) found that in the summer,
forage (tall fescue, orchardgrass, red clover, and
white clover) had higher MET levels than in the
fall (0.31% vs. 0.17%, respectively). They compared broiler performance in diets with/without synthetic methionine and with/without feed
restriction. They concluded that the ability of forage to meet the MET requirement depends on
Page 8
Diet Formulations
Sample diet formulations for broilers, layers, and
turkeys are provided in Possibilities and Limitations of Protein Supply in Organic Poultry and Pig
Production (Sundrum, 2005). However, many of
the ingredients are nonorganic, such as potato
protein and corn gluten meal, and these cannot
be used in the United States.
See Table 3 for fishmeal-based organic broiler
diets designed by West Virginia University for
research using both slow- and fast-growing broilers. No synthetic MET is used, and all the ingredients are available in the United States.
upplying
sufficient MET
to birds with
plant proteins,
such as soybeans or
sunflower meal, may
result in diets with
excessive protein
levels, which can be
harmful to both
the birds and the
environment.
See Table 4 for starter and grower organic meatchicken diets from Europe using organic sunflower, sesame, and rapeseed, which can be difficult to obtain in the United States. Note that
the MET and MET+CYS levels are lower than
those recommended by the NRC.
Trials
The Methionine Task Force and its members
have conducted feeding trials to test various
diets. For example, Organic Valley did trials with
high-MET corn and presented a poster at the 1st
IFOAM International Conference on Animals in
Organic Production (seeAppendix). Some trials
included additional betaine in an attempt to spare
or reduce the need for some MET.
The Methionine Task Force has also sponsored
research to develop a natural methionine product. In addition, the Task Force commissioned
Page 9
Conclusions
It is likely that organic poultry will not have
enough methionine in the diet without synthetic
Item
Starter formulation1
Grower formulation
Finisher formulation
Slow-growing
broiler (028 d)
Slow-growing
Fast-growing
broiler (2856 d) broiler (2138 d)
Slow-growing
Fast-growing
broiler (5683 d) broiler (3854 d)
Fast-growing
broiler (021 d)
Ingredient (%)
Corn
Soybean meal (44%)
2
Sand
38.33
38.33
54.90
54.90
65.55
65.55
43.91
52.08
36.19
34.45
26.24
24.95
6.22
Soybean oil
4.53
1.86
5.00
3.02
3.14
5.00
4.00
5.00
0.25
5.00
2.00
2.08
Limestone
1.18
1.16
1.33
1.05
1.17
1.00
Dicalcium phosphate
1.10
0.86
1.53
0.82
1.11
0.66
Salt
0.42
0.41
0.51
0.46
0.49
0.46
Vitamin-mineral premix3
0.20
0.20
0.20
0.20
0.20
0.20
Choline
0.10
0.10
0.10
0.10
0.10
0.10
2,918
2,918
3,215
3,131
3,209
3,167
25
29
20
22
18
19
Methionine
0.44
0.52
0.35
0.43
0.34
0.38
TSAA
0.82
0.95
0.68
0.77
0.63
0.68
24
27
22
24
21
23
0.41
0.45
0.33
0.38
0.32
0.37
Calculated nutrient
ME (kcal/kg)
CP
Analyzed nutrient
CP
MET
1
Washed builders sand was used to dilute nutrients in the Slow-growing diets so that corn percentages could remain constant among Slow-growing and Fastgrowing diets.
Supplied per kilograms of diet: vitamin D3, 1,361 ICU; vitamin A, 3,629 IU; vitamin E, 9.07 IU; vitamin K, 0.679 mg; thiamine, 0.453 mg; riboflavin, 2.27 mg; niacin, 18.1
mg; pantothenic acid, 4.54 mg; pyridoxine, 0.907 mg; folacin, 0.227 mg; biotin, 0.011 mg; vitamin B12, 0.005 g; calcium, 0.093 g; sodium, 0.025 mg; potassium, 1.4
mg; magnesium, 26.5 mg; sulfur, 61.7 mg; manganese, 9.82 mg; zinc, 20.58 mg; iron, 8.23 mg; copper, 2.06 mg; iodine, 0.411 mg; selenium, 0.039 mg.
Page 10
Corn
20
15
Wheat
37
43.5
Sunflower
Pea
Rapeseed
Soybean meal
24
22
Sesame
Soy oil
2.5
2.5
0.5
Crude protein
20.5
19.7
MET
0.34
0.32
MET+CYS
0.71
0.675
Minerals/vitamins
Organic acid
Calculated feed composition (%)
References
Ambrosen, T., and V.E. Petersen. 1997. The influence of
protein level in the diets on cannibalism and quality of
plumage of layers. Poultry Science. Vol. 76, No. 4. p.559-563.
Cole, D.J.A., and T.A. van Lunen. 1994. Ideal Amino Acid
Patterns. p. 99-112. In: Amino Acids in Farm Animal Nutrition. CAB International, Wallingford, Oxfordshire, U.K.
Baker, D.H. 1997. Ideal amino acid profiles for swine and
poultry and their application in feed formulation. BioKyowa
Technical Review. No. 9. p. 1-24.
Page 12
Appendix A
Evaluation of Organic Broiler
and Layer Ration Formulations
Introduction
ingredient under the organic standards. The primary ingredients in current poultry rations are
corn and soybean meal. Corn (0.16% available
methionine) is extremely low in available methionine and soybean meal (0.61% available methionine) can be considered as moderate in its available methionine content. For these reasons, it is
the standard in poultry rations that supplemental
synthetic methionine is added. These synthetic
sources typically are DLmethionine (99% available methionine) and HMB (Alimet, 88% available methionine). Due to the available methionine
potencies of the supplements, the typical inclusion
rate is three to six pounds per ton of the ration
(0.15 to 0.3% of the ration). For the rations in
this example, organic soybean oil was also used
when necessary to allow more formulation freedom, even though many organic feed producers
do not use soybean oil (the oil is usually supplied
to the ration in the form of full-fat soybean meal).
he basis
for feed
formulation
is developing the
nutritional matrix
that is the backbone
for each ingredient.
t appears
that the use of
fishmeal alone
as an alternative
ingredient is not
sufficient to replace
the synthetic
methionine.
Conclusions
This series of formulations demonstrates that
synthetic methionine supplements cannot simply
be removed from the rations and the remaining
ingredients reformulated. The resulting imbalances would be detrimental to the birds health.
It appears that the use of fishmeal alone as an alternative ingredient is not sufficient to replace the synthetic methionine. It did appear that potentially
feasible rations could be developed with the use
of alternative ingredients. The primary constraint
of this last conclusion is that these ingredients are
either non-existent or of extremely limited availability when they are forced to fully comply with
the organic ingredients standards. And again it
must be noted that these conclusion were reached
using only two average rations. Other nutritionists will likely experience additional deficiencies/
excesses when formulating specific rations.
Source: Methionine Alternative Task Force, 2003
Appendix B
Available Methionine Content of Ingredients for Poultry (%)
Page 14
Appendix C
Broiler Ration Formulation Evaluation
Ration (lbs. per ton)
A
Corn
1065
170
160
874
950
Soybean Meal
855
1720
1690
800
582
Soybean Oil
21
66
60
DL Methionine
Other
55
44
40
56
58
Fishmeal
50
50
220
245
Potato Protein
Earthworm Meal
65
Sesame Meal
100
1350
1250
1250
1350
1350
Nutritional Comparison
Metabolizable Energy (kcal/lb)
Protein, %
Available Methionine, %
21
38
39
29
28
0.49
0.45
0.49
0.49
0.49
102
108
25
11
Appendix D
Layer Ration Formulation Evaluation
Ration (lbs. per ton)
A
Corn
1090
245
455
1075
1310
Soybean Meal
645
1498
1245
465
40
Soybean Oil
58
56
57
DL Methionine
Other
204
201
193
215
210
50
50
50
Fishmeal
Corn Gluten Meal
195
125
Potato Protein
65
Earthworm Meal
100
Sesame Meal
100
1325
1165
1215
1325
1325
Nutritional Comparison
Metabolizable Energy (kcal/lb)
Protein, %
Available Methionine, %
18
34
31
22
20
0.40
0.40
0.40
0.40
0.40
107
88
www.attra.ncat.org
Page 15
Appendix E
Alternatives to Synthetic Methionine Feed Trial
Ingredient
239
Ingredient
lbs
Ingredient
275
organic hi methionine
240
organic hi methionine
organic hi methionine
128
corn
140
corn
corn
13
organic soymeal
organic soymeal
organic soymeal
13
organic flaxmeal
12
organic flaxmeal
12
organic flaxmeal
50
50
50
50
organic oats
50
organic oats
50
organic oats
organic barley
organic barley
organic barley
calcium
calcium
dical phos
dical phos
105
dical phos
potato starch
potato starch
105
potato starch
calcium
Purpose
Control Group
Background 1
Three groups of cornish cross broiler cockerels are being given
different rations. Each group is receiving the same amount of
feed each day. Growing conditions are similar, all three groups
are being raised on the same organic farm, in separate adjoining pens. Each batch is being weighed twice/week to measure
growth patterns. Chicks arrived on May 10 as day-olds from
Sunny Hatchery in Beaver Dam, WI. Changed/refilled waterers twice daily. Feed was given twice a day (splitting the total
grams/day) except for the week of June 9 through 17, 1 X/day.
1
Page 16
Hi-Meth Corn
Highest energy level.( Initial feeding frenzies lessened when
feed was increased above recommended levels.) Initially,
noticed a few birds with fecal matter covering vent as chicks.
(After we physically removed the matter it did not reappear.) Gained weight fastest, but Control Group caught up
Organic Poultry Production: Providing Adequate Methionine
Potato Protein
Grew the slowest and never caught up with the Control or
Hi Meth Corn groups, leading one to believe that it would
take longer to grow out a broiler, or raise a layer to begin
www.attra.ncat.org
Conclusion
High-Methionine Corn is a potential alternative to synthetic
methionine in organic poultry rations.
Page 17
Notes
Page 18
Notes
www.attra.ncat.org
Page 19
Page 20