GC Operational Qual L19
GC Operational Qual L19
GC Operational Qual L19
GC HARDWARE
OPERATIONAL QUALIFICATION
Headspace tests apply only if a headspace sampler is an integral part of the system; Injection Carry Over is included
in the standard OQ for GCs with headspace configurations but not for liquid sampler configurations (it can be ordered
as EXTRA COST TEST).
Key:
Fixed setpoints/limits
Variances allowed
Test
Limits
N/A
GC Oven Temperature
Accuracy and Stability
Temperature 1 = 230.0 C
Accuracy 1.0% of setpoint (in K)
Temperature 2 = 100.0 C
Stability 0.5 C
Stability measured at temperature 2
N/A
Zone 1: 100.0 C
Zone 2: 110.0 C
Zone 3: 115.0 C*
Temperature 1: 60.0 C
Setpoints for temperature 2 and 3
are variable
Accuracy 10.0%
Precision 5.0%
Detector signal
Noise 0.10 pA
Drift 2.50 pA/hour
Detector signal
Noise 0.15 DU
Drift 2.20 DU/hour
Noise 0.15 DU
Drift 3.50 DU/hour
Initial signal
(N/A for 5890)
Noise 0.15 DU
Drift 1.00 DU/hour
Noise 3.00 DU
Drift 15.00 DU/hour
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Test
Limits
Noise 5.00 DU
Drift 5.00 DU/hour
Noise 4.00 DU
Drift 3.00 DU
Initial signal, phosphorous
(N/A for 5890)
Noise 5.00 DU
Drift 5.00 DU/hour
Noise 2.00 DU
Drift 1.50 DU
Signal to Noise
(FID/non-SS/using 1871060170)
Signal to Noise
(FID/non-SS/using 5188-5372)
S/N 300
Signal to Noise
(FID/SS/ALS/MMI)
S/N 5,000
S/N 100
S/N 1,500
Injection Precision
(Split/Splitless)
Injection Precision
(Purged/Packed)
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The OQ test design incorporates modular and holistic testing which is a proven and regulatory acceptable approach. Direct
metrology is used to test the inlet integrity (pressure decay and pressure accuracy), detector flow accuracy and temperature
accuracy of the GC (oven, oven ramp, inlet/detector) and headspace heated zones. Holistic chemical testing is used for the
evaluation of the following critical instrument characteristics: precision, signal to noise, and carry over. Certified reference
standards and calibrated traceable thermometers and manometers are used. Considering the number of set points, parameters,
and conditions of each recommended OQ test, the proven concepts of worst case, range, and representative have been
applied. If a property or characteristic is known to have its worst performance at one end of a range of use, that end is the
setpoint that should be tested and other setpoints are not required. If a property or characteristic has no known worst case,
testing at the high and low points of the range of use is required. If there are too many possible use cases and conditions to
realistically test and none is a worst case, a representative sample for test is the best approach.
The following OQ tests for GC systems (with FID, ECD, uECD, TCD, NPD, FPD, but NOT MS) will be performed as appropriate
for the configuration of the individual GC system.
Headspace Leak
Description: Proper operation of the valves is critical for repeatable peak areas and carry over.
Procedure: This test verifies that the valves operate properly with no excessive leaks or restricted internal flow paths.
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Column flow is achieved by maintaining a constant pressure against a known restriction. Because the restriction is a function
of the column geometry, measuring pressure in the inlet is the most accurate way to determine flow.
Procedure: The inlet is capped, a pressure is applied, and the inlet pressure is recorded using an external calibrated manometer
connected to the inlet.
Signal to Noise
Description: Sensitivity of GC detection is a critical performance feature in quantitative and qualitative analysis. A signal-tonoise value of a representative compound at known concentration provides sensitivity statistics.
Procedure: A traceable standard is injected and signal to noise is calculated.
Injection Precision
Description: System precision is critical for quantitative analysis.
Procedure: An initial stabilizing injection is made, followed by six repeat injections of a traceable standard followed by a final
blank injection. The %RSD of the six injections is calculated to provide precision statistics. There are separate dedicated
instrument parameters and reference standards applicable to each inlet/detector combination. This test is performed with
liquid and headspace sampler configurations.
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www.agilent.com/chem/enterprise
Information, descriptions and specifications in this
publication are subject to change without notice.
Agilent Technologies, Inc. 2015
Published in USA
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