BD Stem Cell Resource

Markers of Self-Renewal and Differentiation

23-9953-02
Self Renewing Pathway
TGF-/Activin Sperm Liver Thymus Pancreas Thyroid Lung Intestine
Skin and Hair JAK-STAT
Wnt
Wnt
BMP-SMad
BMP4
Erk-MAPK
bFGF
TGF-/Activin

LIF

Egg
LIFR gp130

p
p p
Jak Smads 175 MEK/ERK
-catenin

Primordial Germ Cell

Stat3
C-myc Sox2 Oct4 Nanog Smads 2/3

Neural Crest
Neural Stem Cell
Self Renewal
Stem Cell
Ectoderm Embryonic Stem Cell
or Induced Pluripotent Endoderm
Neurons, Glia
Smooth Muscle
Stem Cell
Cardiac Tissue
Chondrocytes
Glial Restricted Progenitor
Osteocytes

Neuronal
Type 1 Astrocyte Oligodendrocyte Restricted
Progenitor
Progenitor
Mesoderm Mesenchymal
Stem Cell

Type 2 Astrocyte
Endothelium
Oligodendrocyte Neuron
Heart Skeletal Muscle Kidney Smooth Muscle
Myoblast Adipocyte (Fat)
Chondrocyte Fibroblast
Osteoblast (Bone) (Cartilage)
Hemangioblast

Myotube
(Muscle) Plasmacytoid
Hematopoietic Stem Cell Committed Lymphoid Progenitor
Dendritic Cell

Monoblast Megakaryoblast
Proerythroblast Pre-NK Cell Thymocyte Pre B Cell
Myeloblast

Erythroblast Progranulocyte
Monocyte Myeloid Dendrtitic Cell Megakaryocyte
NK Lymphoblast T-Lymphoblast B-Lymphoblast

Normoblast Neutrophilic Eosinophilic Basophilic

Myelocyte Myelocyte Myelocyte
NK Cell T Cell B Cell
Macrophage
Thrombocytes Reticulocyte Neutrophilic Eosinophilic Basophilic
(Platelets) Band Cell Band Cell Band Cell

Plasma Cell

Erythrocyte Neutrophil Eosinophil Basophil

(Red Blood Cell)

White colored markers are available from BD Biosciences

Ectoderm Markers Embryonic Stem Cells Mesoderm Markers Endoderm Markers Stem Cell Relevant Signaling
Astrocytes Neuronal Markers Embryonic Stem Cell Markers Early Mesoderm Markers Heart - Cardiogenesis Markers Hematopoietic Stem Cell Markers Mesenchymal Stem Cell Differentiation Markers Mesenchymal Stem Cell Markers (Fat) Definitive Endoderm Markers Erk-MAPK Signaling TGF Beta Signaling
A2B5 GFAP -Synuclein MAP2B Alkaline Phosphatase Nanog (Human) BMP2 Cryptic -Actinin CD309 (VEGFR2, KDR) Negative Markers Chondrocyte (Cartilage) Markers Osteoblast (Bone) Markers Negative Markers B-Catenin HNF-1 (TCF-2) basic FGF Mek1 BMPR-II Id3
CD44 Ran-2 -Synuclein (pY125) MASH1 CD9 Nanog (Mouse) BMP4 MIXL1 Annexin VI CD325 (N-Cadherin) CD184 (CXCR4) HNF-3 (FoxA2) C-Raf Mek1 (pS298) CD119 (IFN- receptor -chain) p16 INK4
FGFR3 S100B b-Tubulin (Tuj1) mGluR1 CD15 (SSEA-1, Lewis X) Nucleostemin Brachyury NF-YA Cardiac specific myosin Connexin-43 CD2 CD24 Aggrecan Matrillin-4 Alkaline Phosphatase IGFBP-3 CD11b (Integrin M chain) CD79a CD325 (N-Cadherin) Sox17 ERK Mek1/2 (pS222) c-Myc p107
CD24 mGluR1 CD24 Oct3/4 CD31 (PECAM1) Nodal Cardiac Troponin I Desmin CD3 CD33 CD44 Osteonectin Collagen 1 Osteocalcin CD14 CD117 (SCF R, c-kit) GATA4 ERK1 Mek2 DP-1 p300
Early Ectoderm Markers CD56 (NCAM) Nestin CD29 (Integrin 1) Oct3/4A CD34 (Mucosialin, gp 105-120) Sca-1 (Ly6A/E) Cardiac Troponin T Flk-1 (KDR, VEGF-R2, Ly-73) CD4 CD36 CD151 Sox9 Fibronectin RUNX2 CD19 HLA-DR ERK1/2 (pT202/pY204) p38 MAPK (PT180/pY182) E2F-1 PP2A Catalytic
Nestin TP63 CD81 (TAPA-1) NeuroD1 CD30 Podocalyxin CD325 (N-Cadherin) Snail Caveolin-2 GATA4 CD7 CD38 Collagen II CD45 (Leukocyte Common Hepatic Endoderm Markers ERK2 Ras E2F-2 ROCK-I
Notch1 CD90 (Thy-1) Neurofilament NF-M CD49f (Integrin 6) Podocalyxin Associated Caveolin-3 GATA6 CD8 CD45 (Leukocyte Common Antigen, Ly-5) ERK3 E2F-3 ROCK-II
Myoblast (Muscle) Markers CD90 (Thy-1)
CD90.1 (Thy-1.1) Neuron-restricted progenitors CD133 (AC133, Prominin-1) Keratin Sulfate Endothelial Cell Markers CD13 HAND1 CD10 Antigen, Ly-5)
Positive Markers
-Fetoprotein
Id1 Smad2/3
Neural Crest Cell Markers CD90.2 (Thy-1.2) Neuropilin-2 CD194 (CCR4) PRC2 (Suz12) CD14 CD151 CD31 (PECAM1) HAND2 CD11b CD45R (B220) Acetylcholinesterase Lactate Dehydrogenase (LDH) Albumin CD326 (EpCAM) Jak-STAT Signaling Id2 SP1
CD13 CD48 Actin Myf-5 CD9 CD73 (Ecto-5-nucleotidase) ASGPR 1 GATA4
CD49d (Integrin 4) MASH1 CD112 Nicastrin CD324 (E-Cadherin) Rex-1 CD15 (SSEA-1, Lewis X) CD152 (CTLA-4) CD34 (Mucosialin, gp 105-120) Islet 1 CD130 (gp130) STAT4
CD57 (HNK-1) Neurogenin 3 CD271 (p75, NGFR/NTR) p150 Glued CD326 (EpCAM) Ronin CD18 (Integrin 2 chain, CR3/CR4) CD157 CD66 M-Cadherin CD14 CD56 (NCAM) B-Enolase (ENO-3) MyoD CD10 CD90 (Thy-1) CD26 HNF-1
STAT1 (C-TERMINUS) STAT4 (pY693)
Wnt Signaling
CD15 (SSEA-1, Lewis X) CD97 CD146 (MCAM, MUC18) Pax3 CD13 CD105 (Endoglin) CD29 (Integrin 1) HNF-1 (TCF-2)
CD271 (p75, NGFR/NTR) Notch1 CD325 (N-Cadherin) Pax-5 CD338 (ABCG2) SFRP2 CD29 (Integrin 1) CD166 (ALCAM) CD66c MLC2a STAT1 (pY701) STAT5 (MGF) 14-3-3 GSK-3
CD15s (Sialyl Lewis x) CD114 (G-CSF Receptor) CD325 (N-Cadherin) Pax7 CD29 (Integrin 1) CD106 (VCAM-1) CD44H (Pgp-1, H-CAM) LDLR
ChAT P-glycoprotein c-Myc Smad2/3 CD31 (PECAM1) CD184 (CXCR4) CD106 (VCAM-1) MLC2v STAT3 STAT5 (MGF) (PY694) -Catenin GSK-3 (pY216)
CD49f (Integrin 6) Tat-SF1
Neural Stem Cell Markers Contactin PSD-95 Cripto Sox2 CD34 (Mucosialin, gp 105-120) CD192 (CCR2) CD117 (SCF R, c-kit) Myogenin CD16 CD138 (Syndecan-1) Desmin PK-M CD44 CD146 (MCAM, MUC18) STAT3 (pS727) STAT6 -Catenin HDAC3
Doublecortin Serotonin Receptor 5-HT 2AR DPPA4 SSEA-3 CD140a (PDGFRa) Myosin Heavy Chain CD19 CD235a CD49d (Integrin 4) CD166 (ALCAM)
A2B5 CD349 (Frizzled-9)
GABA B Receptor Serotonin Receptor 5-HT 2BR DPPA5 (ESG1) SSEA-4
CD36 CD201 (EPCR)
CD144 (VE-cadherin) NF-YA CD20 GATA3 Mesenchymal Stem Cell Markers (Bone Marrow) CD49e (Integrin 5) Fibronectin Intestinal Stem Cell Markers STAT3 (pY705) STAT6 (pY641) -Catenin Jagged1
BMI-1 Ki-67 CD45 (Leukocyte Common CD202b (TIE2) -Catenin Lgr5
CD15 (SSEA-1, Lewis X) MSI1 (Musashi-1)
Gad65 SMN FGF-4 SSEA-5 Antigen, Ly-5) CD202b (TIE2) (pY992) CD166 (ALCAM) Nkx2.5
Positive Markers Negative Markers Positive Markers
CD54 (ICAM-1) HLA I BMI-1 Lgr5 Notch Signaling Akt Neurogenin 3
GAP-43 (Neuromodulin) Synapsin I FoxD3 STAT3 CD172a (SIRPA) P-glycoprotein (MDR) CD55 Vimentin EphB2 Sox9 CaM Kinase II Nicastrin
CD15s (Sialyl Lewis x) MSl2 (Musashi-2) CD45R (B220) CD202b (TIE2) (pY1102) CBP (CREB-Binding Protein) Notch1
GluR 2 Synaptophysin Frizzled-5 STAT3 (pS727) CD202b (TIE2) SSEA-1 BMI-1 CDw93 (C1qRp) CD11a (Integrin L chain) CD34 (Mucosialin, gp 105-120) CD10 CD117 (SCF R, c-kit) CD59 Casein Kinase I Notch1
CD49d (Integrin 4) CD209
CD24 Nanog (Mouse)
GluR2 Synaptotagmin GCNF (NR6A1) STAT3 (pY705) CD202b (TIE2) (pY992) Vimentin CaM Kinase IV CDw338 (ABCG2) CD11b (Integrin M chain) CD45 (Leukocyte Common CD13 CD120a (TNF Receptor Type I) Pancreatic Endoderm Markers Jagged1 Notch2
Casein Kinase II PP2A Catalytic
CD29 (Integrin 1) Nestin
GluR5/6/7 Syntaxin GCTM-2 STAT3-interacting protein 1
CD49e (Integrin 5)
CD49f (Integrin 6)
CD209a (CIRE, DC-SIGN)
CD213a1 CD202b (TIE2) (pY1102) CD31 (PECAM1) DAZL CD14 Antigen, Ly-5) CD14 CD120b (TNF Receptor Type II) Skeletal Muscle Markers CD49f (Integrin 6) Neurogenin 3
Jagged2 Notch3
Casein Kinase II SNAI2 (Slug)
CD49f (Integrin 6) Noggin Neurogenin 3 Notch4
Glutamine Synthetase Tau GDF-3 TRA-1-60 Antigen CD54 (ICAM-1) CD213a2 CD34 (Mucosialin, gp 105-120) Flk-1 (KDR, VEGF-R2, Ly-73) CD19 CD79a CD15 (SSEA-1, Lewis X) CD146 -Actinin Myf5 CD142 Neuropilin-2 CBP (CREB-Binding Protein) Wnt16
CD54 (ICAM-1) Notch1
CD81 (TAPA-1) Pax-6
HNF-3b (FoxA2) TrkB Hsp27 TRA-1-81 Antigen CD56 (NCAM) CD249
Hemangioblast Markers CD40f (Integrin a6 chain) FOXO1 CD31 (PECAM1) HLA-DR CD29 (Integrin 1) CD166 Actin MyoD Chromogranin A Nkx2.2
Nicastrin
Islet-1 Tubby Lefty-A UTF-1 CD62E (E-Selectin) CD252 (OX-40 Ligand) ACE CD324 (E-Cadherin) CD44 G-CSF CD44 (Pgp-1, H-CAM) CD166 (ALCAM) B-Enolase (ENO-3) Myogenin Gad65 Nkx6.1
CD95 (Fas/APO-1) Prominin-2
Jagged1 Tyrosine Hydroxylase Lin-28 Zic3 CD62L (L-Selectin) CD253 (TRAIL) Brachury EphB4 CD59 Gfi-1 CD49d (Integrin 4) CD271 (p75, NGFR/NTR) CD146 (MCAM, MUC18) Myosin heavy chain Glucagon Pancreatic polypeptide
CD133 (AC133, Prominin-1) PSA-NCAM
MAP2 (a+b) Vimentin CD62P (P-Selectin) CD262 (TRAIL-R2, DR5) CD31 (PECAM1) Flk-1 (KDR, VEGF-R2, Ly-73) CD90 (Thy-1) HOXB4 CD49e (Integrin 5) Flk-1 (KDR, VEGF-R2, Ly-73) CD325 (N-Cadherin) Myosin light chain Glut2 Pax-6
CD146 (MCAM, MUC18) Sox1 Induced Pluripotency Markers CD102 CD322 CD34 (Mucosialin, gp 105-120) GATA1 CD90.1 (Thy-1.1) Jak2 CD51 (Integrin V) GD2 Desmin Titin Insulin PDX-1
CD184 (CXCR4)
CD271 (p75, NGFR/NTR)
Sox2
Vimentin
Oligodendrocyte Markers c-Myc Nanog CD105 (Endoglin) CD325 (N-Cadherin) CD133 (AC133, Prominin-1) LMO-2 CD105 (Endoglin) Lmo2 CD54 (ICAM-1) Ly-6A/E (Sca-1) M-Cadherin Islet-1 PTF1A
CD338 (ABCG2) CD44 O1 KLF4 Oct3/4 CD106 (VCAM-1) CDw93 (C1qRp) CD144 (VE-cadherin) Podocalyxin CD106 (VCAM-1) Ly-6A/E (Sca-1) CD56 (NCAM) Nucleostemin Islet-2 Somatostatin
CD44H (Pgp-1, H-CAM) O4 Lin-28 Sox2 CD109 CDw145 CD202b (TIE2) Runx1 (CBFA2) CD117 (SCF R, c-kit) MRP1 CD71 (Transferrin Receptor) SSEA-4 Smooth Muscle Markers NeuroD1 Synaptophysin
CD140a (PDGFR ) Olig1 CD112 Flk-1 (KDR, VEGF-R2, Ly-73) CD202b (TIE2) (pY992) Tal (SCL) CD133 (AC133, Prominin-1) NF-YA CD73 (Ecto-5-nucleotidase) STRO-1
Galactocerebroside C Olig2 Primordial Germ Cell Markers CD116 (GM-CSF Receptor) HIF-1 CD202b (TIE2) (pY1102) CD164 Notch1 CD90 (Thy-1) TAZ
Calmodulin Smooth muscle myosin Primitive Gut Tube Markers
Desmin Vimentin
MBP RIP CD117 (SCF R, c-kit) IP-10 CD184 (CXCR4) P-glycoprotein CD105 (Endoglin) Vimentin CDX2 HNF-1 (TCF-2)
Blimp-1 Nanos CD201 (EPCR) Runx1 (CBFA2) CD106 (VCAM-1)
CD120a (TNF Receptor Type I) Ly-6A/E (Sca-1)
CD15 (SSEA-1, Lewis X) Oct3/4
Skin Precursor Cell Markers CD117 (SCF R, c-kit) Piwi/Hiwi
CD120b (TNF Receptor Type II) Nestin CD202b (TIE2) Sox7
CD121a (IL-1 Receptor, Type I/p80) STAT3 CD202b (TIE2) (pY992) Tal (SCL)
CRABP2 Sca-1 (Ly6A/E) DAZL Steel
CD124 (IL-4 Receptor ) STAT3 (pS727) CD202b (TIE2) (pY1102) WASP (Wiskott-Aldrich
Fibronectin Vimentin FABP9 Stella (Dppa3)
CD141 (Thrombomodulin) STAT3 (pY705) CD325 (N-Cadherin) Syndrome Protein)
Nestin Fragilis VASA (DDX4)
CD144 (VE-cadherin) STAT3-interacting protein 1
Visual System Trophoblast Markers CD146 (MCAM, MUC18) Vimentin
CD147 (Neurothelin)
CD68 Pax-6 CDX2 Sox7
CD325 (N-Cadherin) Recoverin Cytokeratin 7 Trop-2

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