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    MBS-FTA Card Sample Collection Instruction

    Uploaded by

    Gandi Sogandi
    This document provides instructions for collecting bacterial, blood, and overnight culture samples on FTA cards for PCR analysis. For bacterial colonies, three single colonies are resuspended and applied to each card, while overnight cultures use 125 ul applied per card. Whole blood is directly applied to cards before allowing all samples to dry completely before sealing and shipping at room temperature.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    MBS-FTA Card Sample Collection Instruction

    Uploaded by

    Gandi Sogandi
    60 views2 pages
    This document provides instructions for collecting bacterial, blood, and overnight culture samples on FTA cards for PCR analysis. For bacterial colonies, three single colonies are resuspended and applied to each card, while overnight cultures use 125 ul applied per card. Whole blood is directly applied to cards before allowing all samples to dry completely before sealing and shipping at room temperature.

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    FTA card collection instruction

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    This document provides instructions for collecting bacterial, blood, and overnight culture samples on FTA cards for PCR analysis. For bacterial colonies, three single colonies are resuspended and applied to each card, while overnight cultures use 125 ul applied per card. Whole blood is directly applied to cards before allowing all samples to dry completely before sealing and shipping at room temperature.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    60 views2 pages

    MBS-FTA Card Sample Collection Instruction

    Uploaded by

    Gandi Sogandi
    This document provides instructions for collecting bacterial, blood, and overnight culture samples on FTA cards for PCR analysis. For bacterial colonies, three single colonies are resuspended and applied to each card, while overnight cultures use 125 ul applied per card. Whole blood is directly applied to cards before allowing all samples to dry completely before sealing and shipping at room temperature.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    1st BASE Pte Ltd First BASE Laboratories Sdn Bhd (604944-X)

    41 Science Park Road, #01-22/23 The Gemini Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
    Molecular Biology Services Singapore Science Park II, Singapore 117610 Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
    T: +65 6775 7318 T: +603 8943 3252
    Sample Collection Instruction F: +65 6775 7211 F: +603 8943 3243
    E: mbs-sg@base-asia.com E: mbs-my@base-asia.com

    INSTRUCTION FOR SAMPLE COLLECTION OF DNA FOR PCR

    (A) Sample Collection of Bacterial Colonies


    1. Pick a single colony from Agar & re-suspend in 10 l of bacterial culture medium, PBS or 1x TE buffer
    2. Label the FTA card provided with Sample Name and Preparation Date
    3. Apply 10 l of bacterial suspension to FTA card
    4. Repeat steps 1 & 3 for another two times, making it a total of 3 colonies of one sample on the FTA card. Use pencil to circle the spot(s).
    Please use one card per sample. Do not apply different samples on the same card.
    5. Allow sample to dry for at least 1 hour at room temperature
    6. Seal card in a ziplock bag and ship at room temperature

    Page 1 of 2
    1st BASE Pte Ltd First BASE Laboratories Sdn Bhd (604944-X)
    41 Science Park Road, #01-22/23 The Gemini Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
    Molecular Biology Services Singapore Science Park II, Singapore 117610 Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
    T: +65 6775 7318 T: +603 8943 3252
    Sample Collection Instruction F: +65 6775 7211 F: +603 8943 3243
    E: mbs-sg@base-asia.com E: mbs-my@base-asia.com

    INSTRUCTION FOR SAMPLE COLLECTION OF DNA FOR PCR

    (B) Sample Collection of Overnight Bacterial Culture

    1. Grow bacteria overnight in medium


    2. Label the FTA card with Sample Name and Preparation Date
    3. Apply approximately 125 l of culture to FTA card
    4. Allow sample to dry for at least 1 hour at room temperature
    5. Seal the card in a ziplock bag and ship at room temperature

    (C) Sample Collection of Blood


    1. Apply whole blood to the FTA card
    2. Label the FTA card with Sample Name and Preparation Date
    3. Allow sample to dry completely at room temperature
    4. Seal the card in a ziplock bag and ship at room temperature

    Page 2 of 2

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