Ascp Boc Questions

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1.

Cat scratch disease – bartonella henslea


2. Microscopic agglutination caused by CAIHA: answers: presence of cold antibodies & infection w/
Mycoplasma pneumoniae

3. Mycoplasma pneumo causes walking pneumonia


4. Latex agglutination staph aureus – clumping & protein A
5. False DECREASE ESR – delay 8 hrs in set up
6. Prolonged apnea – pseudocholinesterase
7. Specimen rotavirus – stool
8. Specimen legionella – urine antigen
9. Cushing’s syndrome – hyperglycemia
10. Increased Ca and normal PTH – metastatic carcinoma
11. Primedone – Phenobarbital
12. Low sodium – hyperglycemia
13. Low sodium – repeat ion selective electrode
14. Low erythropoietin – polycythemia vera
15. PT normal (patient for gall bladder surgery), PTT prolonged, TT normal – factor XII assay
16. They give Ab panel and you identify (lewis Ab) – adsorbed from plasma
17. Cbc result: about method 1, method 2 – lyse resistant in Hgb C
18. quantitative fecal fat test – weight & extraction
19. absent trophozoite / merozoite – PLASMODIUM FALCIPARUM
20. lupus anticoagulant – thrombosis
21. UA results: 25 – 30 renal tubular epi cells  acute tubular necrosis
22. Bacteria LAP (-), bile esculin (+), NaCl (growth), PYR (-) Resistant to vancomycin – leuconostoc
23. Carbon dioxide ion selective electrode measure – pCO2
24. ANA patterned picture – (speckled or nuclear anti-SSA)
25. Monocytosis seen in tuberculosis
26. FBS:120, OGTT: 140 – impaired glucose
27. Patient with fasting blood glucose 155mg/dL & random 225 mg/dL - OGTT
28. Hair perforation test: trichophyton mentagropytes & T. rubrum
29. 18% retics – Heinz body stain
30. 0.1% retics – pure red cell aplasia
31. streptokinase therapy does not work in myocardial infarction – D-dimer positive
32. lesion of arm, cigar bodies – sporothrix schoenkii
33. hct 33%, hct 33.5% in manual – lipemic
34. rbc in reagent strip, none seen in microscope: diluted ALKALINE urine
35. blastoconidia – mother & daughter cells
36. CSF storage in subsequent culture – incubate at 35C temp
37. TSI, A/A, oxidase positive – aeromonas
38. Pink colony on MAC agar, LOA -++: enterobacter cloacae
39. CA 19-9: pancreatic marker
40. Increased hemolytic anemia – increased UNCONJUGATED bili, increased urobilinogen
41. EIA HTLA ½ reactive, what to do next? – western blot
42. False NEGATIVE ABO – red cells positive DAT
43. Anti-IgG NEGATIVE, anti C3D POSITIVE – prewarm saline solution
44. Echinocytes picture – faulty to dry the slide
45. No growth @ 6.5% NaCl – streptococcus bovis (group D), endocarditis & colorectal cancer
46. Specific gravity 1.010 at 4C result glucose 1000mg/dL – correct the temperature due to high
glucose
47. What is the saliva Le(A+ B-) person? – Lea
48. Ph 4.5 in urine – high protein diet
49. Tap water: M. gordonae
50. Mutation of polycythemia vera: JAK
51. Pharyngitis, seen in renal biopsy: s. pyogenes
52. Differentiate p. aeruginosa from p. putida: 42C
53. Valinomycin: potassium
54. Image of crithidia lucilae: double stranded, SLE
55. pH measurement needs: known buffer @ constant temp
56. butchers cut – e. rhusiopathiae
57. many tear drops(PBS), what deficiency? – DNA
58. favors growth of anaerobic GNR – vitamin K & hemin
59. rapid testing for CMV? - PCR DNA urine
60. rouleaux not seen in what phase? AHG
61. Burr cells – uremia
62. Newborn w/o thymus gland, normal B cells but no production of t cells – DiGeorge syndrome
63. PT control out but APTT within normal range – change CaCl2 reagent
64. Organism isolated in Hektoen: TSI K/A, H2S (+), PAD (-), lysine decarboxylase (-), urea(+), citrate
(+)  tech report as NORMAL FLORA
65. Instrument linearity something about comparing means – paired T-test
66. Postprandial lipemia? Triglycerides!!!
67. Whole blood donation stops at 390 mL: pRBC (low volume unit)
68. Le(a) Le(b) IS 37 AHG
0+1+00
0+1+00
+ 0 0 + / - 2W + / - 2W
+ 0 0 +/ -2W +/-2W
glycolipid absorbed from plasma
69. Ab that deteriorates in storage: P1
70. Choose positive controls to test for anti-c and negative control to test anti-Fy(a): C+c+ for
positive control, Fy(a) for negative control
71. Detection of Ab where 11 tubes resulted NEGATIVE in AHG, but when added CC 4 of them didn’t
agglutinate – machine didn’t dispense correctly the saline in the wash
72.
IS 37 AHG CC
SC1 0 0 0 2+
SC2 +/- +/- 0 2+
Answer: add 4 drops of serum
73. patient DAT (4+), IAT (+), did eluate and the results are DAT (2+) they auto absorb serum and
keeps reacting to SCI1 & SC2 in AHG, what should you do? Panel cells (there was also enzyme
panel cells, report DAT or make another autoadsorption)
74.
Anti-A Anti-B Rh Du Control D
0 0 3+ + -
IS 37 AHG CC
SC1 0 0 0 2+
SC2 0 0 0 2+
Patient cells 0, 0, 2+ not tested  presents auto allo Ab
75. calculate % of saturation – UIBC 185, Fe 125, TIBC = 185 + 125 = 310 %sat (125/310) * 100 = 40%
76. PT normal, PTT (56), mix 1: plasma (47)  factor VIII deficiency
77. Sample taken from indwelling catheter, patient isn’t on anticoagulants yet PTT & TT are way
elevated – HEPARIN CONTAMINATION (from catheter)
78. In the second phase of platelet aggregation what is irreversible? Fibrin formation
79. Control and patient’s PTT elevated, control & patient PT elevated: thromboplastin was added by
error
80.
Anti-A Anti-B A B
4+ 4+ 2+ 2+
What should tech do? First, perform Ab screen w/ autocontrol. If screen & autocontrol = negative THEN
Prewarm b/c cold agglutinins
180.
Anti-A Anti-B A B
0 2+mf 4+ 0
Discrepancy due to Bx-subgroup
81. HgbA1C – what can be the trouble with the test???  decreased life span on RBCs (in the case
of sickle cell)
82. Mycoplasma can’t be treated w/ penicillin = no cell wall
83. Effect of dextran as anticoagulant: destroy D antigen
84. Potassium permanganate: quenching agent
85. Common error in PCR: nucleic acid contamination
86. Low incidence Ag present in blood panel: Wra
87. Surfactant fetal lung maturity – phosphatidyl glycerol
88. Anti-microsomal – hashimoto’s thyroiditis
89. In multichannel analyzer, controls of enzymatic assays are lower than expected values while
non-enzymatic assay controls are within normal limits. What is the probable cause? 
instrument temperature may be low
90. Speckled pattern – anti SBB, anti RNP, anti Sm
91. Patient has the results after collecting blood in an indwelling catheter. Patient is not in heparin
/ anticoagulant therapy. APTT: abnormal, PT: normal, fibrinogen: 150 mg/dL, what test should
be ordered?  Factor XII assay
92. PREDOMINANTLY seen in acute phase of infection but rarely seen in chronic infection? anti-
HBc, IgM
93. Adrenal cushing syndrome – TSH decrease, cortisol increase
94. Deferred donor: Hepatitis Ig six months ago

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