RECALLS

1. Cat scratch disease – bartonella henslea

2. Microscopic agglutination caused by CAIHA: answers: presence of cold
antibodies & infection w/ Mycoplasma pneumoniae

3. Mycoplasma pneumo causes walking pneumonia

4. Latex agglutination staph aureus – clumping & protein A
5. False DECREASE ESR – delay 8 hrs in set up
6. Prolonged apnea – pseudocholinesterase
7. Specimen rotavirus – stool
8. Specimen legionella – urine antigen
9. Cushing’s syndrome – hyperglycemia
10. Increased Ca and normal PTH – metastatic carcinoma
11. Primedone – Phenobarbital
12. Low sodium – hyperglycemia
13. Low sodium – repeat ion selective electrode
14. Low erythropoietin – polycythemia vera
15. PT normal (patient for gall bladder surgery), PTT prolonged, TT normal –
factor XII assay
16. They give Ab panel and you identify (lewis Ab) – adsorbed from plasma
17. Cbc result: about method 1, method 2 – lyse resistant in Hgb C
18. quantitative fecal fat test – weight & extraction
19. absent trophozoite / merozoite – PLASMODIUM FALCIPARUM
20. lupus anticoagulant – thrombosis
21. UA results: 25 – 30 renal tubular epi cells  acute tubular necrosis
22. Bacteria LAP (-), bile esculin (+), NaCl (growth), PYR (-) Resistant to
vancomycin – leuconostoc
23. Carbon dioxide ion selective electrode measure – pCO2
24. ANA patterned picture – (speckled or nuclear anti-SSA)
25. Monocytosis seen in tuberculosis
 RECALLS
26. FBS:120, OGTT: 140 – impaired glucose
27. Patient with fasting blood glucose 155mg/dL & random 225 mg/dL - OGTT
28. Hair perforation test: trichophyton mentagropytes & T. rubrum
29. 18% retics – Heinz body stain
30. 0.1% retics – pure red cell aplasia
31. streptokinase therapy does not work in myocardial infarction – D-dimer
positive
32. lesion of arm, cigar bodies – sporothrix schoenkii
33. hct 33%, hct 33.5% in manual – lipemic
34. rbc in reagent strip, none seen in microscope: diluted ALKALINE urine
35. blastoconidia – mother & daughter cells
36. CSF storage in subsequent culture – incubate at 35C temp
37. TSI, A/A, oxidase positive – aeromonas
38. Pink colony on MAC agar, LOA -++: enterobacter cloacae
39. CA 19-9: pancreatic marker
40. Increased hemolytic anemia – increased UNCONJUGATED bili, increased
urobilinogen
41. EIA HTLA ½ reactive, what to do next? – western blot
42. False NEGATIVE ABO – red cells positive DAT
43. Anti-IgG NEGATIVE, anti C3D POSITIVE – prewarm saline solution
44. Echinocytes picture – faulty to dry the slide
45. No growth @ 6.5% NaCl – streptococcus bovis (group D), endocarditis &
colorectal cancer
46. Specific gravity 1.010 at 4C result glucose 1000mg/dL – correct the
temperature due to high glucose
47. What is the saliva Le(A+ B-) person? – Lea
48. Ph 4.5 in urine – high protein diet
49. Tap water: M. gordonae
50. Mutation of polycythemia vera: JAK
51. Pharyngitis, seen in renal biopsy: s. pyogenes
52. Differentiate p. aeruginosa from p. putida: 42C
53. Valinomycin: potassium
54. Image of crithidia lucilae: double stranded, SLE
55. pH measurement needs: known buffer @ constant temp
56. butchers cut – e. rhusiopathiae
57. many tear drops(PBS), what deficiency? – DNA
58. favors growth of anaerobic GNR – vitamin K & hemin
 RECALLS
59. rapid testing for CMV? - PCR DNA urine
60. rouleaux not seen in what phase? AHG
61. Burr cells – uremia
62. Newborn w/o thymus gland, normal B cells but no production of t cells –
DiGeorge syndrome
63. PT control out but APTT within normal range – change CaCl2 reagent
64. Organism isolated in Hektoen: TSI K/A, H2S (+), PAD (-), lysine
decarboxylase (-), urea(+), citrate (+)  tech report as NORMAL FLORA
65. Instrument linearity something about comparing means – paired T-test
66. Postprandial lipemia? Triglycerides!!!
67. Whole blood donation stops at 390 mL: pRBC (low volume unit)
68. Le(a) Le(b) IS 37 AHG
0+1+00
0+1+00
+ 0 0 + / - 2W + / - 2W
+ 0 0 +/ -2W +/-2W
glycolipid absorbed from plasma
69.Ab that deteriorates in storage: P1

70.Choose positive controls to test for anti-c and negative control to test anti-
Fy(a): C+c+ for positive control, Fy(a) for negative control

71.Detection of Ab where 11 tubes resulted NEGATIVE in AHG, but when

added CC 4 of them didn’t agglutinate – machine didn’t dispense correctly
the saline in the wash

72.
IS 37 AHG CC
SC1 0 0 0 2+
SC2 +/- +/- 0 2+
Answer: add 4 drops of serum
73.patient DAT (4+), IAT (+), did eluate and the results are DAT (2+) they auto
absorb serum and keeps reacting to SCI1 & SC2 in AHG, what should you
do? Panel cells (there was also enzyme panel cells, report DAT or make
another autoadsorption)
 RECALLS

Anti-A Anti-B Rh Du Control D

0 0 3+ + -
IS 37 AHG CC
SC1 0 0 0 2+
SC2 0 0 0 2+
Patient cells 0, 0, 2+ not tested  presents auto allo Ab
74.calculate % of saturation – UIBC 185, Fe 125, TIBC = 185 + 125 = 310 %sat
(125/310) * 100 = 40%
75.PT normal, PTT (56), mix 1: plasma (47)  factor VIII deficiency
76.Sample taken from indwelling catheter, patient isn’t on anticoagulants yet
PTT & TT are way elevated – HEPARIN CONTAMINATION (from catheter)
77.In the second phase of platelet aggregation what is irreversible? Fibrin
formation
78.Control and patient’s PTT elevated, control & patient PT elevated:
thromboplastin was added by error
79.
Anti-A Anti-B A B
4+ 4+ 2+ 2+
What should tech do? First, perform Ab screen w/ autocontrol. If screen &
autocontrol = negative THEN Prewarm b/c cold agglutinins
180.
Anti-A Anti-B A B
0 2+mf 4+ 0
Discrepancy due to Bx-subgroup
80.HgbA1C – what can be the trouble with the test???  decreased life span
on RBCs (in the case of sickle cell)
81.Mycoplasma can’t be treated w/ penicillin = no cell wall
82.Effect of dextran as anticoagulant: destroy D antigen
83.Potassium permanganate: quenching agent
84.Common error in PCR: nucleic acid contamination
85.Low incidence Ag present in blood panel: Wra
86.Surfactant fetal lung maturity – phosphatidyl glycerol
87.Anti-microsomal – hashimoto’s thyroiditis
 RECALLS
88.In multichannel analyzer, controls of enzymatic assays are lower than
expected values while non-enzymatic assay controls are within normal
limits. What is the probable cause?  instrument temperature may be low
89.Speckled pattern – anti SBB, anti RNP, anti Sm
90.Patient has the results after collecting blood in an indwelling catheter.
Patient is not in heparin / anticoagulant therapy. APTT: abnormal, PT:
normal, fibrinogen: 150 mg/dL, what test should be ordered?  Factor XII
assay
91.PREDOMINANTLY seen in acute phase of infection but rarely seen in
chronic infection? anti-HBc, IgM
92.Adrenal cushing syndrome – TSH decrease, cortisol increase
93.Deferred donor: Hepatitis Ig six months ago