JABATAN PELAJARAN NEGERI SELANGOR

PERSIDANGAN KEBANGSAAN PENGETUA SEKOLAH MENENGAH

PROGRAM PENINGKATAN PRESTASI AKADEMIK

SIJIL PELAJARAN MALAYSIA 2010

MARK SCHEME

BIOLOGI PAPER 3
Question 1
1 (a) [KB0603 - Measuring Using Number]
Score Criteria
3 Able to record all the time taken for the starch to be hydrolysed completely in
the boxes provided accurately.
Sample answers:
Test tube P Q R
Time taken (minutes) 18 12 10
2 Able to record any two readings accurately.

1 Able to record any one reading accurately.

0 Not able to response or wrong response.

1 (b) (i) [KB0601 - Observation]

Score Criteria
3 Able to state any two observations correctly according to the criteria:

 Test tube P / Q / R // Concentration of enzyme amylase

 Time taken for the starch to be hydrolysed completely// no change of iodine
colour

Sample answers:
Horizontal;
1. In test tube P/ 0.5% amylase enzyme, the time taken for completely
hydrolysed / no change of iodine colour is 18 minutes.
2. In test tube R/ 1.5% amylase enzyme, the time taken for completely
hydrolysed/ no change of iodine colour is 10 minutes.

Vertical

3. The time taken for the starch to hydrolysed completely/ no change of iodine
colour in R /1.5% amylase enzyme is the shorter than in P / 0.5% amylase
enzyme

4. The time taken for the starch to hydrolyse completely/ no change of iodine
colour using P enzyme amylase is the longer than in R

2 Able to state any one observation correctly.

or
Able to state any two incomplete observations (Any 2 criteria).

Sample answers:

Horizontal;
1. In test tube P, the time taken is long for the starch to hydrolyse completely/
no change of iodine colour
In test tube R, time taken is short for the starch to hydrolyse completely/ no
change of iodine colour

Vertical;
2. The time taken for the starch to hydrolyse completely using 0.5% enzyme

PERATURAN PEMARKAHAN 2
4551
 amylase is shorter.
3. The time taken for the starch to hydrolyse completely using 0.5% enzyme
amylase is shorter
1 Able to state any one idea of observation (Any 1 criterion)

Sample answers:
1. Different time is taken for the starch to hydrolyse completely.
2. The time taken varied in this experiment.

0 Not able to response or wrong response.

1 (b) (ii) [KB0604 - Making inferences]

Score Criteria
3 Able to make one logical inference for each observation based on the
criteria:
 Concentration is high/low
 Rate of enzyme reaction

Sample answers:
Horizontal;
1. (In test tube P), the concentration of enzyme is low so rate of enzyme
reaction is slow
2. (In test tube R), the concentration of enzyme is high so rate of enzyme
reaction is fast

Vertical;
3. When the concentration of enzyme is higher the rate of enzyme reaction is
faster than in lower concentration of enzyme.
4. When the concentration of enzyme is lower the rate of enzyme reaction is
slower than in higher concentration of enzyme

2 Able to make one logical inference for any one observation.

or
Able to make one logical and incomplete inference base on 2 criteria for
each observation.

Sample answers:

1. In test tube R , rate of enzyme reaction is fast/ high

2. In test tube P, rate of enzyme reaction is slow/low
3. Different concentration of enzyme, different rate of enzyme reaction
4. Rate of enzyme reaction depends on the concentration of enzyme

1 Able to make an idea of inference with one criterion.

Sample answers:
1. The rate of enzyme reaction varied.
2. The concentration amylase affects the reaction.
3. Different rate of enzyme amylase reaction in different test tube.
4. The longer the time the less the activity of enzyme amylase.

0 Not able to response or wrong response

PERATURAN PEMARKAHAN 3
4551
1 (c) [KB061001 - Controlling Variables]
Score Criteria
3 Able to state all the variables and the method to handle the variables
correctly.
Sample answers:

Variables Method to handle the variables

Manipulated variable: Three enzyme concentrations are used that
Enzyme concentration are 0.5%, 1.0% and 1.5% enzyme amylase
solutions.// use different concentration of
enzyme

Responding variable: The time taken for the starch to hydrolyse is

The time taken for the starch recorded by using a stopwatch.
to hydrolyse completely / // Calculating the rate of enzyme amylase
disappear activity as;
// The rate of enzyme 1
amylase activity.
time taken for starch hydrolyses completely

Controlled variable: 2 ml of enzyme amylase solution is used in

Volume of enzyme// each test tube // 1% of starch suspension is
concentration/ volume of used for each test tube / all test tubes are
starch / temperature of water immersed in 37C of water bath.
bath

2 Able to state 4 - 5 answers

1 Able to state 2 - 3 answers

0 Able to state 1 answer , not able to response or wrong response

1 (d) [KB0611 - Making Hypothesis]

Score Criteria
3 Able to state a hypothesis to show a relationship between the manipulated
variable and responding variable, based on 3 criteria:
 Manipulated variable (MV)
 Responding variable (RV)
 Relationship (R)

Sample answers:
1. In 0.5% enzyme amylase solutions the time taken for the starch to
hydrolyse completely is the longest / the rate of enzyme amylase activity is
the lowest / minimum.
2. As the concentration of enzyme amylase increases, the time taken for the
starch to hydrolyse completely decreases / the rate of enzyme amylase
activity increases.

2 Able to state less accurate hypothesis to show a relationship between

manipulated variable and responding variable base on 2 criteria.

PERATURAN PEMARKAHAN 4
4551
 Sample answers:
1. In 0.5% enzyme amylase solutions the time taken for the starch to
hydrolyse completely is the long / the rate of enzyme amylase activity is the
low. (1 point only, no comparison made)
2. Test tube Q has the highest rate of enzyme amylase activity. (No MV)
3. Different concentrations of enzyme solutions give different rate of enzyme
amylase activity. (No R)
4. The concentration of enzyme affects the rate of enzyme amylase activity.
(No R)
5. The time taken for the starch to hydrolyse completely depends on different
concentration of enzyme. (No R)

1 Able to state idea of hypothesis to show a relationship between manipulated

variable and responding variable base on 1 criterion.

Sample answers:
1. The time taken depends on the concentration of enzyme.
2. The activity depends on the concentration of enzyme.

0 Not able to response or wrong response

1 (e) [KB0606 - Communicating]

Score Criteria
3 Able to tabulate a table and fill in data accurately base on three criteria:
 Enzyme concentration (%)
 The time taken for starch hydrolysed completely (minute)
 Rate of enzyme amylase activity (minute-1)

Sample answers:
Enzyme The time taken Rate of enzyme
concentratio for starch to hydrolyse amylase activity
n (%) completely (minute) (minute-1)
0.5 18 0.055 / 0.056 / 0.06
1.0 12 0.083 / 0.0833 / 0.08
1.5 10 0.10 / 0.1

2 Able to tabulate a table base on two criteria.

1 Able to Able to tabulate a table base on one criterion.

0 Not able to response or wrong response

1 (f) [KB0608 - Space and Time Relationship]

Score Criteria
3 Able to plot the graph of the rate of enzyme amylase activity against enzyme
concentration based on three criteria:
 Both axes with correct units and labels
 All points are plotted correctly
 Smooth curve / straight line touching all points.

2 Able to plot the graph of the rate enzyme amylase activity against enzyme
concentration based on any two criteria.

PERATURAN PEMARKAHAN 5
4551
 1 Able to plot the graph of the rate enzyme amylase activity against enzyme
concentration based on any one criterion.

0 Not able to response or wrong response

PERATURAN PEMARKAHAN 6
4551
he rate of enzyme amylase activity
( minute-1 )

0.11

0.10

0.09

0.08

0.07

0.06

0.05

0.04

0.03

0.02

0.01

0
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6
Enzyme concentration ( % )
Kepekatan enzim ( % )

PERATURAN PEMARKAHAN 7
4551
1 (g) [KB0607 - Interpreting Data]
Score Criteria
3 Able to state clearly and accurately the relationship between:
 The concentration of enzyme and the rate of enzyme amylase activity
 More enzyme molecules in the test tube
 More chances of enzyme-substrate collision/ complex form.

Sample answer:
1. The higher the concentration of enzyme solution, the higher the rate of
reaction, cause by more enzyme molecules in the test tube. More chances
of collision occur between enzyme and starch / enzyme substrate-
complexes are more formed.

2 Able to state clearly but less accurate the relationship base on 2 criterion:

Sample answers:
1. The higher the concentration of enzyme solution, the higher the rate of
reaction, cause by more enzyme molecules in the test tube.

1 Able to state the idea of the relationship base on 1 criterion

Sample answer:
1. The higher the concentration of enzyme solution, the higher the rate of
reaction.

0 Not able to response or wrong response

1 (h) [KB0609 - Define Operationally]

Score Criteria
3 Able to define operationally what an enzyme is base on experiment
according to the criteria:
 the hydrolysis of starch (in 37C)
 product that make iodine solution remains unchanged / yellow
 The rate of reaction is affected by enzyme concentration

Sample answer:
1. A substance/amylase that hydrolyse starch in 37 C into a product that make
the iodine solution remains unchanged / yellow, and the rate of reaction is
affected by the enzyme concentration.

2 Able to state what an enzyme is base on experiment less accurately

according to one of the criteria.

Sample answers:
2. An enzyme is use to hydrolyse starch.
3. The rate of reaction is affected by enzyme concentration.

1 Able to state the idea of an enzyme or the theoretical definition of enzyme.

Sample answers:

PERATURAN PEMARKAHAN 8
4551
 1. Enzyme affects the hydrolysis of starch.
2. The activity of enzyme is affected by concentration.
3. An enzyme is a biological catalyst which speeds up the rate of reaction (in a
cell).
0 Not able to response or wrong response

1 (i) [KB0605 - Predicting]

Score Criteria
3 Able to predict the result accurately.

 Long time/ unable to record the time for changes

 High temperature
 Enzyme denatured/ active site destroyed.

Sample answers:
1. 30-40 minutes / longer than 30 minutes / longer than the time taken when
kept at 37oC, in high temperature, enzymes are denatured / the active sites
for the reaction are destroyed (to act on the substrate / starch).

2 Able to predict the result with 2 criteria:

Sample answers:
1. 30-40 minutes / the iodine solution drops always turn blue / longer than 30
minutes / longer than the time taken when kept at 37oC.
2. The enzymes are denatured / the active sites for the reaction are destroyed
(to act on the substrate / starch).

1 Able to give only predicted result.

Sample answers:
1. The time taken is longer/ any value longer than 30 minutes.

0 Not able to response or wrong response

1 (j) [KB0602 - Classifying]

Score Criteria
3 Able to categorise all the materials and type of medium correctly.
Sample answer:

Materials Type of medium

Bahan Jenis medium

 Sodium hydroxide solution alkaline

Larutan natrium hidroksida

 Hydrochloric acid acidic

Asid hidroklorik

 Distilled water Neutral

Air suling

PERATURAN PEMARKAHAN 9
4551
 2 Able to categorise two materials and type of medium correctly.

1 Able to categorise one material and type of medium correctly.

0 Not able to response or wrong response

PERATURAN PEMARKAHAN 10
4551
Question 2

Problem Statement
Score Criteria
3 Able to state the problem statement of the experiment correctly that include
criteria:
 Manipulate variables
 Responding variables
 Relation in question form and question symbol [?]

Sample answers:
1. Does the concentration of carbon dioxide affect the rate of photosynthesis
in aquatic plants?
2. What is the relationship between the concentration of carbon dioxide and
the rate of photosynthesis in aquatic plants?

2 Able to state the problem statement of the experiment with two criteria.

Sample answers:
1. Does carbon dioxide concentration affect the photosynthesis in aquatic
plants?
2. Does carbon dioxide affect the rate of photosynthesis in aquatic plants?
3. What is the relationship between carbon dioxide concentration and the
photosynthesis of aquatic plants?

1 Able to state the of problem statement with one criteria.

Sample answers:
1. Does carbon dioxide affect the photosynthesis in plants?

Hypothesis
Score Criteria
3 Able to state the hypothesis correctly according to the criteria:
 Manipulate variables
 Responding variables
 Relationship of the variables

Sample answers:
1. The higher the concentration of carbon dioxide the higher the rate
photosynthesis in aquatic plants.
2. At (very) high concentration of carbon dioxide, the rate of photosynthesis in
aquatic plants remains constant.

2 Able to state the hypothesis with two criteria

Sample answers:
1. The higher the carbon dioxide concentration, the higher the photosynthesis
in plants.
2. The carbon dioxide concentration affects the rate of photosynthesis in

PERATURAN PEMARKAHAN 11
4551
 aquatic plants.
3. The higher the carbon dioxide the higher the rate of photosynthesis in
aquatic plants.

1 Able to state the idea of the hypothesis.

Sample answers:
1. The concentration of carbon dioxide affects the photosynthesis in plants.

Variables
Score Criteria
3 Able to state the three variables correctly

Sample answers:
Manipulated variable: Concentration of carbon dioxide // Percentage of
sodium hydrogen carbonate solution
Responding variable: Number of bubbles released (in five minutes) // The
rate of photosynthesis
Controlled variable: Type of (aquatic) plant / Hydrilla sp. // Volume of
(hydrogen carbonate) solutions

Materials and Apparatus

Score Criteria
3 Able to state all functional materials / 2*materials + 1 other material and
2*apparatus + 2 other apparatus for the experiment.

Materials: *Aquatic plants / Hydrilla sp., * sodium hydrogen carbonate

solution, distilled water, and plasticine.
Apparatus: *Stopwatch, measuring cylinder, ruler, boiling tube / test tube,
beaker, filter funnel, capillary tube, rubber tubing, light source /
box, and paper clip.

2 Able to state all functional materials / 2*materials and 2*apparatus + 1 other

apparatus for the experiment.

1 Able to state all functional materials / 2*materials and 2*apparatus for the
experiment.

Procedure
Score Criteria

PERATURAN PEMARKAHAN 12
4551
 3 Able to state five procedures P1, P2, P3, P4 and P5 correctly.
P1 : How to Set Up The Apparatus (3P1)
P2 : How to Make Constant The Control Variable (1P2)
P3 : How to Manipulate The Manipulated Variable (1P3)
P4: How to Record The Responding Variable (2P4)
P5 : Precaution (1P5)

2 Able to state three of any procedures: 3P1 / 1P2 / 1P3 / 2P4 / 1P5 correctly

1 Able to state two of any procedures: 3P1 / 1P2 / 1P3 / 2P4 / 1P5 correctly

0 Able to state only one of any procedures, not able to response or wrong
response

Example of Procedure:
1. (Diagram of experimental setup with at least 5 functional labels). P1
2. Fill a boiling tube with 150 ml P2
of 1% sodium hydrogen carbonate solution.
3. Fix a paper clip / plasticine to a sprig of aquatic / Hydrilla sp. plant. P1
4. Submerge / put the aquatic plant into the boiling tube / 1% sodium hydrogen P1
carbonate solution.
5. Put the boiling tube into a water bath at room temperature (20-30oC) P5
6. Place the boiling tube P1
at distance of 50 cm from a lighted bulb. P2
7. Put aside the boiling tube for 5 minute (for the aquatic plant to adjust) P5
8. Start the stopwatch. P1
9. Count the number of bubbles release in 20 minutes. P4
10. Record the result is a table. P4
11. Repeat steps 2 until 9 using 2%, 3% and 4% of sodium hydrogen carbonate P3
solutions.
12. Calculate the rate of photosynthesis. P4

Data

PERATURAN PEMARKAHAN 13
4551
Score Criteria
Able to tabulate the correct table with observations.
Bonu Sample answers:
s Concentration of sodium Number of bubbles Rate of
hydrogen carbonates released photosynthesis
2m solution (%) in 20 minutes (number / minute)
1
2
3
4

Or

Beaker Number of bubbles released Rate of photosynthesis

in 20 minutes (number / minute)
A
B
C
D

END OF MARKING SCHEME

PERATURAN PEMARKAHAN 14
4551