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Biochemistry 2 s16

This document contains questions for a Biochemistry exam on Molecular Biology and rDNA Technology. It includes questions asking students to discuss in detail: 1) experiments that deciphered the genetic code and the role of EF-Tu, EF-Ts, and EF-G in protein biosynthesis, 2) the structure and working of plasmid pBR322, 3) genomic and cDNA libraries, and 4) restriction enzymes. It also includes short answer and multiple choice questions testing knowledge of topics related to the genetic code, protein synthesis, recombinant DNA technology, PCR, and applications like Bt-cotton.

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Shrut Somkuwar
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34 views2 pages

Biochemistry 2 s16

This document contains questions for a Biochemistry exam on Molecular Biology and rDNA Technology. It includes questions asking students to discuss in detail: 1) experiments that deciphered the genetic code and the role of EF-Tu, EF-Ts, and EF-G in protein biosynthesis, 2) the structure and working of plasmid pBR322, 3) genomic and cDNA libraries, and 4) restriction enzymes. It also includes short answer and multiple choice questions testing knowledge of topics related to the genetic code, protein synthesis, recombinant DNA technology, PCR, and applications like Bt-cotton.

Uploaded by

Shrut Somkuwar
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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TKN/KS/16 – 5928 OR

Sixth Semester B. Sc. Examination Discuss in detail the structure and working of pBR 322.
10
BIOCHEMISTRY
Paper - II 4. Describe in detail the genomic and cDNA libraries.
10
(Molecular Biology and rDNA Technology)
OR
Time : Three Hours ] [ Max. Marks : 50
Write notes on :—
N. B. : All questions are compulsory and carry equal (a) Calcium–phosphate precipitation method of
marks. transformation. 5
1. Discuss in detail the experiments conducted for (b) Blue–White screening. 5
deciphering the genetic code. 10
OR 5. Sovle any 10 of the following :—
Write brief notes on :— (i) State the first codon that was deciphered and the
(a) Error correction in amino acylation. 5 amino acid that it codes for. 1
(b) Shine and Dalgarno sequence. 5 (ii) State any two characteristics of the genetic code.
1

2. With the help of suitable diagrams discuss in detail the (iii) Amino acid is attached to which end of tRNA?
role of : EF–Tu, EF–Ts, and EF–G in protein 1
biosynthesis. 10 (iv) Which subunit of the ribosome has the enzyme to
synthesize the peptide bond ? 1
OR
(v) Which three codons indicate stop signal for protein
Write brief notes on :— biosynthesis ? 1
(a) The roles of release factors RF1 and RF2. 5
(vi) In which of the two sites in the ribosome is the
(b) Formation of 70s initiation complex. 5 initiating codon lodged ? 1
(vii)What is the meaning of the term 'Shuttle vector"?
3. Write an essay on restriction enzymes. 1

TKN/KS/16 – 5928 Contd. TKN/KS/16 – 5928 2 Contd.

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(viii) Which of the two DNA ligases, E.coli DNA
ligase or T4 DNA ligase, is normally used in
recombinant DNA technology ? 1
(ix) Give one difference between linkers and adaptors.
1
(x) Which enzyme is generally used for DNA
polymerization in PCR process ? 1
(xi) Give one major application of southern blotting.
1
(xii) What is meant by 'Bt' in Bt–cotton ? 1

TKN/KS/16 – 5928 3 750

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