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    Unsaturated Iron Binding Capacity (UIBC) Test Kit: (Ferene)

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    Guneyden Guneyden
    This document summarizes an Unsaturated Iron Binding Capacity (UIBC) test kit that uses a chromogenic agent and two reagents to determine the amount of unsaturated iron binding capacity in human serum. The test involves adding reagents to a serum sample and calibrator, measuring the absorbance, and calculating the difference in absorbance to determine the UIBC concentration compared to the calibrator. The normal reference range for UIBC is 31-51 μmol/L. Precautions and performance characteristics are provided.

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    Unsaturated Iron Binding Capacity (UIBC) Test Kit: (Ferene)

    Uploaded by

    Guneyden Guneyden
    226 views1 page
    This document summarizes an Unsaturated Iron Binding Capacity (UIBC) test kit that uses a chromogenic agent and two reagents to determine the amount of unsaturated iron binding capacity in human serum. The test involves adding reagents to a serum sample and calibrator, measuring the absorbance, and calculating the difference in absorbance to determine the UIBC concentration compared to the calibrator. The normal reference range for UIBC is 31-51 μmol/L. Precautions and performance characteristics are provided.

    Original Description:

    Salah

    Original Title

    UIBC

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    This document summarizes an Unsaturated Iron Binding Capacity (UIBC) test kit that uses a chromogenic agent and two reagents to determine the amount of unsaturated iron binding capacity in human serum. The test involves adding reagents to a serum sample and calibrator, measuring the absorbance, and calculating the difference in absorbance to determine the UIBC concentration compared to the calibrator. The normal reference range for UIBC is 31-51 μmol/L. Precautions and performance characteristics are provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    226 views1 page

    Unsaturated Iron Binding Capacity (UIBC) Test Kit: (Ferene)

    Uploaded by

    Guneyden Guneyden
    This document summarizes an Unsaturated Iron Binding Capacity (UIBC) test kit that uses a chromogenic agent and two reagents to determine the amount of unsaturated iron binding capacity in human serum. The test involves adding reagents to a serum sample and calibrator, measuring the absorbance, and calculating the difference in absorbance to determine the UIBC concentration compared to the calibrator. The normal reference range for UIBC is 31-51 μmol/L. Precautions and performance characteristics are provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Unsaturated Iron Binding Capacity (UIBC) Test Kit

    (Ferene)

    【NAME】 【OPERATION STEPS】


    Unsaturated Iron Binding Capacity (UIBC) Test Kit (Ferene) R1: Reagent 1 R2: Reagent 2 S: Calibrator U: Sample
    【INTEND USE】 R2: 60µl
    This reagent is intended for the in vitro quantitative determination of
    Unsaturated Iron Binding Capacity (UIBC) in human serum.
    【METHODOLOGY】
    The excess iron ions are present in the alkaline buffer and serum NO and
    iron binding of transferrin, combined with iron all, remaining ferric ion and
    reducing agent, chromogenic agent role after the generation of the blue
    calculate ∆A=A2-A1
    complex, at the wavelength of 600 nm detection. By calculating the
    Incubation time 5min Reaction time 5min
    amount of iron ions in the buffer solution, we can calculate the amount of
    0.9% sodium chloride solution, Recording absorbanceA2
    the unsaturated iron binding capacity. Recording absorbance A1
    U or S:24μl; R1:240μl value
    value
    【STABILITY AND STORAGE】 【CALCULATION】
    Unopened, avoid light preservation in 2 ~ 8 ℃, valid for 12 months; Use The Calibrator
    Opened, avoid contamination preservation in 2 ~ 8 ℃, valid for 1 month. Sample △A
    Reagent is not allowed frozen. Sample UIBC concentration = —————— × Calibrator concentration
    【SPECIMEN COLLECTION AND HANDLING】 Calibrator △A
    It is best to fresh non-hemolytic serum, and avoid mixed with erythrocyte. 【REFERENCE RANGE】
    Sample stability: 2～8℃ preservation stability in 1 week; 31～51μmol/L By clinical trials, choose no less than 100 newborn or
    -20℃preservation stability in 1 month. adults blood specimens, tested by automatic biochemical analyzer, and
    When the lipid emulsion concentration of sample≤300mg/L, bilirubin then processing the testing value with statistical method, calculating out
    concentrations ≤ 600mg/L, hemoglobin hemoglobin ≤ 150mg/L, VC ≤ the reference range.
    500mg/L,Cu2+≤50μmol/L,Zn2+≤80μmol/L, was not observed clearly Recommendation: The laboratory set up its own reference range!
    disturbance. 【THE LIMITATION OF TEST RESULTS】
    【APPLICABLE INSTRUMENT】 Unsaturated Iron Binding Capacity (UIBC) testing is just one of the
    Fully automatic biochemical analyzer standard that clinicist diagnose the patient. Clinical physicians should
    【SYSTEM PARAMETERS】 according to patients' bodies, history and other diagnostic program, to get
    The following system parameters are recommended. Individual instrument comprehensive judgment.
    applications are available upon request from the Technical Support Group 【THE INTERPRETATION OF TEST RESULTS】
    Temperature 37°C Human error, the processing of specimen, analysis instrument deviation,
    Cuvette light path 1.0cm etc. all can affect the measurement result；When one sample deviates from
    Primary Wavelength 600nm the expected value too far, need to be tested again.
    Secondary Wavelength 700nm 【PERFORMANCE INDEX】
    Assay Type Two Point End 1.Reagent blank absorbance ≤0.3,(600nm,lcm optical path).
    Direction Increase 2.Precision: repeatability CV≤10％;batch variations R≤10％.
    Sample: Reagent 1: Reagent 2 Ratio 2:20: 5 3.Accuracy: relative deviation ≤10％.
    eg : Sample Vol. 24 µL 4.Linearity range: 0～80μmol/L,r≥0.990.
    Reagent1 Vol. 240 µL 5. Stability: All package reagent, non corrosive gas and avoid light,
    Reagent2 Vol. 60 µL preservation in 2~8 ℃, stable 12 months.
    Linearity 0～80μmol/L 【ATTENTION】
    Testing Deducting the reagent blank 1. Reagent contains sodium azide (toxic) preservatives, avoid contact with
    skin and mucous membrane. If necessary preventive measures should be
    taken use of reagents, reagent contact with skin and mucous membrane,
    please rinse with water, please go to a doctor if necessary.
    2. The maximum linearity is 80μmol/L. If testing results is upper limit,
    dilute with 0.9% sodium chloride solution before test, results multiplied by
    the dilution ratio.
    3. Liquid waste disposal: Suggest follow local regulations.
    4. This specification is applied to the double reagent.
    5. Different batches reagents cannot mix, when replacing reagents batch
    number, please calibration again.

    Biobase Biodustry(Shandong)Co.,Ltd
    Add: Biobase Biological Industrial Park, Mingshui Economic Development Zone, Jinan, China, 250200
    Tel: 0086-531-81219801/03 Fax: 0086-531-81219804

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