Gujarat Technological University: Chemical Engineering (05) SUBJECT CODE: 2160509

GUJARAT TECHNOLOGICAL UNIVERSITY
CHEMICAL ENGINEERING (05)

BIOTECHNOLOGY
SUBJECT CODE: 2160509
B.E. 6th SEMESTER
Type of course: Department Elective – I
Prerequisite: Basics of Biology and Chemistry
Rationale: This subject is an integration of chemistry with biological systems. It deals with basic
aspects of biological systems, their operations and their applications.
Teaching and Examination Scheme:

Teaching Scheme Credits Examination Marks Total
L T P C Theory Marks Practical Marks Marks
ESE PA (M) ESE (V) PA
(E) PA ALA ESE OEP (I)
4 0 2 6 70 20 10 20 10 20 150
Sr. Content Total %

No. Hrs. Weightage
Introduction to Biotechnology and rDNA technology.
Introduction, Old and New Biotechnology, an Interdisciplinary Activity,
Scope and Importance, Commercial Potential, Biotechnology in India.
1 5 7
Introduction to rDNA technology and genetic engineering, bastic
techniques and tools. Applications of rDNA technology.
Animal Biotechnology
Introduction, The Immune System, Monoclonal Antibodies and Cell
2 Culture Products, In Vitro Fertilization and Embryo Transfer, Babies of 6 8
a Specified Sex, Animal Cloning, Genome Maps, DNA Fingerprinting
in Forensic Medicine
Plant Biotechnology
Introduction, History of Tissue Culture Techniques, General Techniques
3 and terminologies. Different culturing techniques, micropropogation, 5 7
Somaclonal variation, Germplasm conservation.
Biotechnology and Heath care

Introduction, Disease Prevention (Vaccines), Disease Diagnosis,
4 Disease Treatment, Gene Therapy, Immunodeficiency’s, Cancer 417, 6 8
Forensic Medicine.
5 General and Industrial Microbiology 12 17

Introduction, Historical Landmarks, Isolation and Screening of
Microorganisms, Maintenance of Isolates/Strains, Inoculum
Development, Sterilization, Strain Improvement, Process Development,
Downstream Processing, In Situ Recovery of Products, Applications,
Metabolite Production, Biotransformation, Recovery of Metals,
Biocontrol Agents, Biofertilizers, Genetically Engineered Microbes
(GEMS)
Enzyme Technology
Introduction, Historical, Coenzymes and Cofactors, Enzymes Vs.
Catalysts, Enzymes Vs. Whole Cells, Production of Enzymes,
Classification and Nomenclature, Chemical Energetics, Mechanism of
6 Enzyme Action, Enzyme Kinetics, Nontraditional Enzymes, 8 11%
Immobilization of Enzymes, Uses of Enzymes in Solution, Uses of
Immobilized Enzymes, Enzyme Reactors, Biosensors, Enzyme
Engineering, Bi- and Poly –Functional Enzymes, Safety and Regulatory
Aspect.
Foods and Beverages

Introduction, Fermented Foods, Cheese Production, Use of enzymes in
7 Food Industry, Use of Lactase in Dairy Industry, Enzymes in fruit Juice 7 10
and Brewing Industries, Microbial Biomass, Mushrooms, Single Cell
Protein
Fuel Biotechnology
Introduction, Useful Features of Biofuels, Undesirable Features of
8 Biofuels, Areas for Future Research Focus, Energy Crops, Modes of 5 7
Utilization of Biomass, Biogas, Bioethanol, Biobutanol, Biodiesel,
Biohydrogen
Environmental Biotechnology
Introduction, Wastes and Pollutants, Hazards from Wastes and
9 Pollutants, Waste Treatment, Landfill, Aerobic Waste Water Treatment, 8 11
Anaerobic Treatment of Waste Water, Biodegradation of Xanobiotic
Compounds, Bioremediation, Water Quality
Biosafety
Introduction, Historical 615, Definitions, Objectives of Safety
10 Guidelines, Risk Assessment, Containment, Planned Introduction of 5 7
Genetically Modified Organisms (GMOs) Biosafety during Industrial
Production, Biosafety Guidelines in India.
Intellectual Property Rights

Introduction, Intellectual Property, Protection of Intellectual Property
11 Rights, Choice of IPR Protection, International Harmonization of Patent 5 7
Laws, Protection of Biotechnological Inventions, Plant Breeder’s Rights
(PBR), Management of IPR, Benefits from IPR, Problems From IPR.
Suggested Specification table with marks (Theory):
Distribution of Theory Marks

R Level U Level A Level N Level E Level C Level
10 20 15 10 10 05
Legends: R= Remembrance; U= Understanding; A= Application; N = Analyze; E = Evaluate, C

= Create and above Levels (Revised Bloom’s Taxonomy)
Note: This specification table shall be treated as a general guideline for students and teachers. The
actual distribution of marks in the question paper may vary slightly from above table.
List of Experiments:
1. To perform monochrome staining.
2. To perform callus culture
3. Screeing of potential micro organism
4. Sterilization techniques
5. Development of Inoculum
6. Production of bioethanol through fermentative method.
7. Production of biomass in laboratory
8. Basic techniques for waste water analysis.
Reference Books:
1. Basic Biotechnology by Colin Ratledge and Bjorn Kristiansen, Cambridge University Press,
3rd Edition.
2. Biotechnology by B. D. Singh, Kalyani Publisher.
3. Principles of Fermentation Technology, by Whitaker, Peter F Stanbury, S. Hall and A.
Whitaker, Publisher: Butterworth-Heinemann; 2nd edition.
4. Plant Biotechnology by Dr. P. K. Gupta, Rastogi Publication.
Course Outcome:
After learning the course, the students should be able to:
1. Develop a fundamental understanding interdisciplinary approach of Biotechnology and
Chemical Engineering.
2. Understand different branches of biotechnology and its applications in real field.
Open Ended Problems:

Students are free to select any project related to Biochemical engineering based on its application in the
field of Biotechnology. Some of the suggested projects are:
1. To design downstreaming process using fundamentals of industrial biotechnology and
chemical engineering.
2. To perform biodegredation of chemical compouns using combination of chemical and
biological methods.
List of Open Source Software/learning website:

Students can refer to video lectures available on the websites including NPTEL. Students can refer
to the CDs which are available with some reference books. Students can develop their own
flowsheets for demonstration of various fermentation processes and the downstreaming process.
ACTIVE LEARNING ASSIGNMENTS: Preparation of power-point slides, which include videos,
animations, pictures, graphics for better understanding theory and practical work – The faculty will
allocate chapters/ parts of chapters to groups of students so that the entire syllabus to be covered. The
power-point slides should be put up on the web-site of the College/ Institute, along with the names of
the students of the group, the name of the faculty, Department and College on the first slide. The best
three works should submit to GTU.

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GUJARAT TECHNOLOGICAL UNIVERSITY

CHEMICAL ENGINEERING (05)

Type of course: Department Elective – I

Prerequisite: Basics of Biology and Chemistry

Teaching and Examination Scheme:

Sr. Content Total %

Biotechnology and Heath care

5 General and Industrial Microbiology 12 17

Foods and Beverages

Intellectual Property Rights

Distribution of Theory Marks

Legends: R= Remembrance; U= Understanding; A= Application; N = Analyze; E = Evaluate, C

Open Ended Problems:

List of Open Source Software/learning website:

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