HW Ag

HW Ag HW Ag
E.canis Ab

E.canis Ab E.canis Ab
Lyme Ab
Lyme Ab Lyme Ab
2 drops Anapl. Ab

3) Using a disposable capillary tube, add 20㎕ of whole blood into each sample
Anapl. Ab Anapl. Ab

One Step Dirofilaria immitis Antigen and Ehrlichia canis, Borrelia burgdorferi, HW Ag
hole. Or, add 10㎕ of serum/plasma into each sample hole using a micropipette.
Anaplasma phagocytophilum/platys Antibody Test E.canis Ab

HW Ag HW Ag
Lyme Ab

For veterinary diagnostic use only Anapl. Ab

E.canis Ab

Lyme Ab
E.canis Ab

Lyme Ab
Using a disposable dropper, Interpret at 10 min.
Add 2 drops of sample
Anapl. Ab Anapl. Ab

Anigen Rapid CaniV-4 Test Kit 20㎕ of

whole blood HW Ag
3 drops
10㎕ of
serum/plasma HW Ag
HW Ag

E.canis Ab E.canis Ab
HW Ag HW Ag
E.canis Ab
Lyme Ab Lyme Ab
E.canis Ab E.canis Ab
Anapl. Ab HW Ag
Anapl. Ab

■■ Principles
HW Ag HW Ag Lyme Ab
Lyme Ab Lyme Ab

Anapl. Ab
E.canis Ab

Anapl. Ab
or E.canis Ab E.canis Ab
Anapl. Ab

The Anigen Rapid CaniV-4 Test Kit is a chromatographic immunoassay for the
Lyme Ab Lyme Ab Lyme Ab
HW Ag

Anapl. Ab HW Ag Anapl. Ab
Anapl. AbHW Ag

qualitative detection of Dirofilaria immitis antigen, Ehrlichia canis antibody, Borrelia

E.canis Ab

E.canis Ab E.canis Ab

4) Dispense 3 drops of assay diluents into

2 drop of sample
each sample hole.
Add 3 drops of assay diluents
Lyme Ab
Interpret at 10 min.
burgdorferi antibody and Anaplasma phagocytophilum/Anaplasma platys antibody
Lyme Ab Lyme Ab
Anapl. Ab
Anapl. Ab Anapl. Ab

in canine serum, plasma or whole blood.

The Anigen Rapid CaniV-4 Test Kit has two letters which are test (“T”) line and control
(“C”) line on the surface of device. Test line and control line in the result window are 3 drops 3 drops
not visible before applying any samples. The control line is a reference line which
indicates the test is performing properly. The control line has to appear every time 2 drops
when the test has performed. If the target antigens and/or antibodies are present in
HW Ag HW Ag

HW Ag HW Ag

E.canis Ab E.canis Ab

HW Ag E.canis Ab

sample, a purple test line would appear in the result window.

E.canis Ab
Lyme Ab Lyme Ab

Lyme Ab E.canis Ab Lyme Ab

Anapl. Ab Anapl. Ab

The highly selective recombinant antigen or antibody is used as a capture or Anapl. Ab Lyme Ab Anapl. Ab

2 drop of sample Add 3 drops of assay diluents Interpret at 10 min.

detector in the assay. These are capable of detecting Dirofilaria immitis antigen(HW
Anapl. Ab

5) Start the timer. UsingThe

2 drop of sample
sample
a disposable will flow across
Add 3 drops of assay diluents
dropper, the
Interpret at 10 result
min. window.
Interpret at 10 min. If it does not
Ag), Ehrlichia canis antibody(E.canis Ab), Borrelia burgdorferi antibody(Lyme Ab) and appear after 1minute, add one more drop of assay diluents to the sample hole.
Add 2 drops of sample

Anaplasma phagocytophilum/Anaplasma platys antibody(Anaplasma Ab) in canine

sample with high accuracy. 6) Interpret test results at 15 minutes.
2 drops
HW Ag HW Ag
Do not read after 15 minutes. HW Ag

E.canis Ab E.canis Ab
■■ Materials provided Lyme Ab
2 drops
Lyme Ab
E.canis Ab

Lyme Ab

Reagent 5 Tests/Kit 10 Tests/Kit 20 Tests/Kit ■■ Interpretation of the Result

Anapl. Ab

Anapl. Ab Using a disposable dropper,

HW Ag Anapl. Ab Interpret at 10 min.
Add 2 drops of sample
Anigen Rapid CaniV-4 Test Devices 5 10 20 1) Negative result
E.canis Ab

Lyme Ab

Assay diluents bottles 1 1 1 Only control (“C”) line appears Anapl. Ab

HW Ag

Anticoagulant tubes 5 10 20 HW Ag in the result window.

Using a disposable
HW dropper,
Ag Interpret at 10 min.
Add 2 drops of sample E.canis Ab HW Ag

E.canis Ab E.canis Ab
Disposable capillary tubes (20㎕) 5 10 20
E.canis Ab

Lyme Ab Lyme Ab

Lyme Ab Lyme Ab
Instructions for use 1 1 1
Anapl. Ab

Anapl. Ab
Anapl. Ab Anapl. Ab

♣♣ A black line on the capillary tube is the indicator line for 20㎕. 2) Positive result
Test (“T”) line and control (“C”) line within the result window indicate the
HW Ag HW Ag HW Ag
presence of target antigens and/or antibodies.
HW Ag

E.canis Ab E.canis Ab E.canis Ab

*NOTE: Test stripE.canis
ofAbAnaplasma Ab can't differentiate between A.phagocytophilum
■■ Materials required, but not provided Lyme Ab Lyme Ab Lyme Ab and A.platys:
Lyme Ab a positive result indicates presence of antibodies to A.
phagocytophilum and/or A. platys
1) Timer, Micropipette Anapl. Ab Anapl. Ab Anapl. Ab Anapl. Ab

HW Ag Positive E.canis Ab Positive

■■ Precautions 3 drops HW Ag HW Ag

1) The test kit is for canine use only. Do not useHW Ag

HW Agfor other animals. HW Ag
HW Ag
HW Ag
HW Ag
HW Ag
HW Ag E.canis Ab E.canis Ab

2) The test device is sensitive to humidity asAb well as heat. Perform the
E.canis Ab
E.canis testE.canis Ab
E.canis Ab
E.canis Ab
E.canis Ab E.canis Ab
E.canis Ab
Lyme Ab Lyme Ab

immediately after removing the test deviceLyme from

Lyme
Ab
Ab the foil pouch. Lyme Ab
Lyme Ab
Lyme Ab
Lyme Ab
Lyme Ab
Lyme Ab
HW Ag HW Ag

3) Do not reuse the test components.

Anapl. Ab Anapl. Ab
Anapl. Ab Anapl. Ab Anapl. Ab Anapl. Ab
Anapl. Ab Anapl. Ab Anapl. Ab Anapl. Ab
E.canis Ab E.canis Ab

4) Apply the sample and assay diluents vertically. Lyme Ab Positive

Lyme Ab Anaplasma Ab Positive Lyme Ab

5) Do not touch the membrane in the result window of test device. Anapl. Ab Anapl. Ab

6) Do not use the test kit beyond the stated expiration date marked on the package
HW Ag HW Ag

HW Ag HW Ag HW Ag HW Ag
label. E.canis Ab E.canis Ab
2 drop of sample
E.canis Ab
Add 3 drops of assay diluents
E.canis Ab
Interpret at 10 min. E.canis Ab E.canis Ab

7) Do not use the test kit if the pouch is damaged or the seal is broken. Lyme Ab Lyme Ab
HW Ag
Lyme Ab HW Ag
Lyme Ab HW Ag
Lyme Ab HW Ag
Lyme Ab
8) Do not mix components from different lot numbers because the components in Anapl. Ab Anapl. Ab
E.canisAb
Ab E.canis Ab E.canis Ab E.canis Ab
this kit have been quality control tested asAnapl.
standard batch unit. Anapl. Ab Anapl. Ab Anapl. Ab

9) All samples should be handled as being potentially infectious. Wear protective

Lyme Ab Lyme Ab
3 drops
Lyme Ab
3) Invalid Result Lyme Ab
3 drops
gloves while handling samples. Wash hands thoroughly afterwards.
Anapl. Ab Anapl. Ab Anapl. Ab
If the control (“C”) line does not appear, the result might be considered invalid.
Anapl. Ab

10) Decontaminate and dispose of all samples, reaction kits and potentially The sample should be retested.
contaminated materials safely in accordance with national and local regulations.
HW Ag 2 drops HW Ag HW Ag HW Ag HW Ag HW Ag

HW Ag E.canis Ab HW AgE.canis Ab HW Ag E.canis Ab HW AgE.canis Ab

■■ Storage and Stability

E.canis Ab E.canis Ab

E.canis AbLyme Ab E.canis Ab

Lyme Ab E.canis Ab
Lyme Ab E.canis Ab
Lyme Ab
HW Ag
Lyme Ab Lyme Ab

1) Store the test kit at 2~30°C. DO NOT FREEZE. Lyme Ab Anapl. Ab Lyme Ab Lyme Ab
3 drops
Anapl. Ab Anapl. Ab
E.canis Ab Lyme AbAnapl. Ab
3 drops
2) Do not store the test kit in the direct sunlight.
Anapl. Ab Anapl. Ab Anapl. Ab Lyme Ab
Anapl. Ab
Anapl. Ab
Anapl. Ab

2 drop of sample Add 3 drops of assay

2 drop diluents
of sample Addat310
Interpret drops
min.of assay diluents Interpret at 10 min.
3) The test kit is stable within the expiration date that marked on the package label. Anapl. Ab

■■ Limitations
Using a disposable dropper, of the Test Interpret at 10 min.
■■ Collection and Preparation of Sample Add 2 drops of sample
HW Ag HW Ag HW Ag HW Ag

E.canis Ab 1) Although the Anigen Rapid CaniV-4 Test kit is very accurate for detecting
E.canis Ab E.canis Ab E.canis Ab

1) Whole blood, serum, or plasma should be for this test. Lyme Ab Dirofilaria immitis antigen, Ehrlichia canis antibody, Borrelia burgdorferi antibody
Lyme Ab Lyme Ab Lyme Ab

[Whole blood] Collect the whole blood into the anticoagulant tube (Max. vol.
Anapl. Ab and Anaplasma phagocytophilum/Anaplasma platys antibody a low incidence
Anapl. Ab Anapl. Ab Anapl. Ab

1.5ml) provided. If anticoagulated whole blood is not immediately tested, they of false results can be occurred. Other clinical and/or laboratory tests might
should be refrigerated at 2~8°C and used within 24ofhours.
2 drop sample Add 3 drops of assay
2 drop be required ifInterpret
diluents
of sample questionable
Addat310 min.ofresults
drops are obtained. Interpret
assay diluents As other diagnostic
at 10 min. tests, a
2 drops 3 drops 2 drops 3 dropsshould not be based on the result of a single test, but
[Serum] Collect the whole blood into the collection tube (NOT containing definitive clinical diagnosis
anticoagulants such as heparin, EDTA and sodium citrate), leave to settle for 30 should be diagnosed by the veterinarian after all clinical and laboratory findings
minutes for blood coagulation and then centrifuge blood to get supernatant.
HW Ag
have been evaluated. HW Ag

[Plasma] Collect the whole blood into the collection tube (containing
HW Ag
E.canis Ab
2) The reading window may show a light pink background coloration; this will not
HW Ag HW Ag
E.canis Ab

HW Ag

anticoagulants such as heparin, EDTA and sodium citrate) and then centrifuge
E.canis Ab
Lyme Ab
affect the accuracy of the results.
E.canis Ab E.canis Ab
Lyme Ab

E.canis Ab

blood to get plasma. Lyme Ab

Anapl. Ab
3) BioNote and its distributors cannot be held responsible for the consequences of
Lyme Ab Lyme Ab
Anapl. Ab

Lyme Ab

2) Serum samples should be stored at 2~8°C. For Using a disposable

longer storage,dropper, misuse
Using a disposable
freeze the samples Interpret at 10 min. or misinterpretationInterpret
dropper, of theatresults
10 min. given by the test.
Add 22drops
drops Add 22drops
drops
Anapl. Ab Anapl. Ab Anapl. Ab Anapl. Ab

of sample of sample
at -20°C or below. Avoid repeated freezing and thawing.
2 drop of sample Add 3 drops of assay diluents Addat310
drops
min.of assay diluents
3) Samples containing precipitate may yield inconsistent test results. They must
HW Ag
be 2 drop of sample Interpret
HW Ag
Interpret at 10 min.

clarified prior to assaying. E.canis Ab E.canis Ab

Doc. No.: I2120-4E
4) Hemolyzed or contaminated samples may give erroneous results. Lyme Ab Lyme Ab
Revised date: Jul. 13, 2016
Anapl. Ab Anapl. Ab

■■ Procedure of the Test Using a disposable dropper, Using a disposable

Interpret at 10 min. dropper, Interpret at 10 min.
1) All reagents and samples must be at room temperature
Add 2 drops(15~30°C)
of sample before running Add 2 drops of sample
assay.
2 drops
2) Remove the test device from the foil pouch, and place it on a flat and dry 2 drops
surface.
HW Ag HW Ag

E.canis Ab E.canis Ab

Lyme Ab Lyme Ab