Immunohematology MCQs
Immunohematology MCQs
Immunohematology MCQs
A multiple antibody problem was resolved using enzyme. One of the antibody reactions was
eliminated after treatment. Which of the following antibodies was probably present?
a) Anti-c
b) Anti-I
c) Anti-Jka
d) Anti-Fya
a) R1r
b) R2R2
c) R1R1
d) r’r’
Antigen typing on red cells should not be performed if the patient has been transfused within
the following:
a) 30 days
b) 2 months
c) 3 months
d) 6 months
What reagent would be selected to detect the presence of unexpected red cell antibodies in a
patients serum sample?
a) A1 and B cells
b) Panel cells
c) IgG-sensitized cells
d) Screening cells
Antibodies to the Rh blood group system antigens are usually characterized as:
The term used when two of the same alleles of a gene are inherited from each parent is:
a) Homozygous
b) Allele
c) Heterozygous
d) Systemic
To determine the specificity of a red cell antigen in patient sample, what source of antibody is
selected?
The removal of an antibody from serum or plasma using the individuals own red cells is:
a) Autoadsorption
b) Differential adsorption
c) Neutralization
d) Elution
A patient who has a phenotype group AB, D-negative requires 1 unit of plasma. Which of the
following units of plasma would be best for transfusion?
a) Group A, D-negative
b) Group B, D-positive
c) Group AB, D-positive
d) Group O, D-negative
a) Panel cells
b) Commercial anti-B
c) Lectins
d) Antiglobulin reagents
Part of the daily quality control in the blood bank laboratory is the testing of reagent antisera
with corresponding antigen-positive and antigen-negative red cells. What dose this procedure
ensures?
a) Antibody class
b) Antibody titer
c) Antibody specificity
d) Antibody sensitivity
To determine the presence of red cell antibody in a patient sample, what source of antigen is
selected?
An antibody was detected at the screen at 37 Celsius and did not react at the AHG phase. Which
of the following should be suspected?
a) Anti-s
b) Anti-e
c) Anti-N
d) Anti-Jka
a) Anti-IgG
b) Anti-IgM and IgG
c) Anti-IgG anti anti-C3d
d) Anti-C3d
Which of the following situations may produce ABO discrepancies in the serum testing?
a) Newborn
b) Patient with hypogammaglobulinemia
c) Cold alloantibody
d) All of the above
With which of the following red cell phenotypes would anti-Jka react most strongly?
a) Jk(a-b+)
b) Jk(a+b-)
c) Jk(a+b+)
d) Jk(a-b-)
a) Dolichos biflorus
b) Ulex europaeus
c) Dolichos europaeus
d) Ulex biflorus
A mothers titer has risen above the critical level and the father is unavailable for testing. The
MOST useful procedure to determine the antigen type of fetus is:
Which of the following tests is not necessary when testing a cord blood sample?
a) ABO
b) Rh
c) DAT
d) Antibody screen
a) Anti-Lea
b) Anti-A,B
c) Anti-Fya
d) Anti-kell
A mother in group A, D-negative with anti-D in her serum. Which of the following units should
be selected for an intrauterine transfusion?
a) Group O, D-negative
b) Group O, D-positive
c) Group A, D-negative
d) Gro+up A, D-positive
Which of the following would be the LEAST effective in the control of variables affecting the
results of titration procedures?
The appearance of spherocytes in a baby’s blood smear after delivery is usually associated with:
a) ABO HDFN
b) HDFN caused by anti-D
c) HDFN caused by other IgG antibodies
d) Normal physiologic anemia detected in newborns
If bio data regarding critical titer are available. Which of the following titer values is frequently
used as a cutoff?
a) 8
b) 16
c) 32
d) 64
Select the most common antibody specificity associated with DAT positive cord sample:
a) Anti-A,B
b) Anti-C
c) Anti-D
d) Anti-K
The pathophysiology of HDN is associated with both anemia and bilirubinemia. From a
treatment standpoint, which of the following statements is true regarding the clinical concerns
in these two periods?
In HDN bilirubin levels are generally normal at birth but but begin to elevate during the first few
hours after delivery. Which of the following statements BEST explains the reason for this clinical
feature?
An A-positive mother with anti-Kell (K1) gives birth to a B-negative, Kell(K1) positive infant with
HDN. The physician requests blood for exchange transfusion. Which of the following is MOST
suitable?
Objectives for performing an exchange transfusion include all of the following expect to:
a) Anti-Lea
b) Anti-C
c) Anti-K
d) Anti-S
Which of the following drug is associated with the drug adsorption (drug-dependent)
mechanism in drug-induced immune hemolysis?
a) Penicillin
b) Methyldopa
c) Quinine
d) Acetaminophen
Which of the following are the purposes of using IgG coated ‘’check cells’’ in indirect antiglobulin
testing?
a) IgM
b) IgG
c) IgA
d) IgD
a) Aspirin tablets
b) Corticosteroids
c) Iron supplements
d) Blood transfusion
Warm autoimmune hemolytic anemia is characterized most frequently by which of the following
serologic pattern?
The component best suited for a patient deficient in IgA who has had a previous anaphylactic
reaction is:
a) Whole blood
b) Red blood cells
c) Red blood cells leukocyte reduced
d) Red blood cells washed
If a patients RBC phenotype is D+C+E-c-e+S-s+K-Fy(a-b+)Jk(a-b+), which alloantibodies is the
patient capable of forming?
a) Alloanti-E,s,K,Fya,Jka
b) Alloanti-E,c,K,Fya,Jkb
c) Alloanti-E,c,S,K,Fya,Jka
d) Alloanti-E,c,S,Fyb,Jka
Donath-landsteiner antibodies:
a) Temporally deferred
b) Permanently deferred
c) Deferred if ALT is elevated
d) Deferred for 3 months
Which of the following is true regarding the infusion of fresh frozen plasma?
a) Autoantibody alone
b) Alloantibody alone
c) Both autoantibody and alloantibody
d) All of the above
Which of the following plasma exchange techniques is the MOST specific?
a) Simple exchange
b) Exchange with filtration
c) Exchange with cytofiltiration
d) Exchange with immunoadsorption
What is the most important step in assuring that transfused blood is safe?
In plasma exchange procedures, which of the following statements is true regard to the relative
effectiveness of Ig removal?
Identification of alloantibodies in the serum of a patient with autoantibody is essential for the
selection of blood for transfusion. Which of the following specimens is MOST appropriate to
identify an alloantibody in the serum of an untransfused patient who has a strong autoantibody
that reacts with all panel cells at the anti-human globulin phase of testing?
The process by which bound antibody is removed from red cells and captured in a fluid medium
is called:
a) Elution
b) Auto agglutination
c) Adsorption
d) Allo immunization
If autologous cells are pretreated with ZZAP before adsorption, which of the autoantibodies is
not adsorbed out?
a) Anti-e
b) Anti-Jka
c) Anti-K
d) Anti-C
Even with the absence of prior transfusion or pregnancy, individuals with the Bombay
phenotype will always have naturally occurring:
a) Anti-Rh
b) Anti-Lea
c) Anti-H
d) Anti-o
Autoimmune antibodies directed against the I blood group antigen are produced in:
a) Digitonin acid
b) Glycin-HCL
c) Organic solvents
d) Freeze thaw
A patient has blueing of the hands and feet after cold exposure. The patients hematologic and
serologic findings are as follows: hemoglobin of 10g/dL (100g/L), polychromasia,
hemoglobinemia, hemoglobinuria, an elevated reticulocyte count and a positive DAT C3 only.
The MOST probable explanation for these findings is:
a) Once a week
b) On each new admittance
c) Every 3 days
d) Every time a unit is transfused
Which of the following steps should be included in the preliminary laboratory investigation of a
suspected transfusion reaction?
Of the transfusion reaction types listed, which results in the thrombocytopenia owing to
platelets alloantibody?
a) IHTR
b) TA-GVHD
c) FNHTR
d) PTP
a) Anti-A,B
b) Anti-D
c) Anti-Jka
d) Anti-Lub
Which of the following components is MOST frequently associated with transfusion reactions
due to bacterial contamination?
Hemoglobinuria, hypotension and generalized bleeding are symptoms of which of the following
transfusion reactions?
a) Allergic
b) Circulatory overload
c) Intravascular hemolysis
d) Anaphylactic
e) Extravascular hemolysis
Which of the following patient histories might suggest future transfusions with saline-washed
RBCs?
a) ABO
b) Rh
c) Kell
d) Kidd
All of the following are important actions/tests used in the immediate assessment of possible
intravascular hemolytic transfusion reaction EXPECT:
When a patient receiving platelets transfusion experience purpura, the most likely cause is:
a) RBC alloantibodies
b) Platelet antigens
c) Contaminating leukocytes in the platelet component
d) Platelet antibodies
Acute intravascular hemolysis is MOST commonly associated with antibodies of which blood
group system?
a) ABO
b) Rh
c) Kidd
d) Duffy
Post transfusin purpura following transfusion of platelet or RBC unit is usually caused by:
a) Heparin
b) AS-3
c) Lactated ringers
d) Citrate
a) R2R2
b) R1R0
c) R1R2
d) r’r
a) rr
b) R1R1
c) R2R2
d) R1R2
A donor is tested with Rh antisera; given the following results, what is the most probable Rh
genotype?
Anti-D +
Anti-C +
Anti-E 0
Anti-c +
Anti-e 0
a) R1R1
b) R1r
c) R0r
d) R2r
An antibody to the E antigen was identified in a patient who received multiple transfusions.
What is the most likely phenotype to the patient red cells?
a) R1R1
b) R2R2
c) R1r
d) r’r’
The regulator gene RHAG:
a) is inherited on chromosome 1
b) is responsible for Rhmod phenotype
c) must be inherited to express LW antigens
d) is responsible for the D-deletion phenotype
which blood group system possess the Jsb and Kpa antigens?
a) Duffy
b) Lutheran
c) Kell
d) Kidd
a) Anti-Lua
b) Anti-S
c) Anti-Jka
d) Anti-M
a) Fy(a-b-)
b) Jk(a-b-)
c) Le(a-b-)
d) Lu(a-b-)
Enzyme treated reagent red cells used in antibody identification enhance all of the following
antibodies expect:
a) Anti-K
b) Anti-S
c) Anti-U
d) Anti-N
a) Ficin
b) Albumin
c) PEG
d) DTT
Glycophorin A and glycophorin B posses antigen sites for which blood group system?
a) Duffy
b) Kidd
c) Lewis
d) MNS
Select the antibody that is characteristically clinically insignificant:
a) Anti-Kpb
b) Anti-S
c) Anti-Leb
d) Anti-Fya
a) Rhnull phenotype
b) K0 phenotype
c) U-negative phenotype
d) Absence of the Kx antigen
a) I
b) M
c) PI
d) S
Which of the following antibodies requires the antiglobulin test for the in-vitro detection?
a) Anti-M
b) Anti-P1
c) Anti-U
d) Anti-I
What procedure would help to distinguish between anti-Fya and anti-Jka in an antibody
mixture?
Anti-K:
a) K
b) M
c) Leb
d) D
Reagent antibody screening cells may not detect antibodies directed against low-incidence
antigens. Which antibody is most likely to go undetected?
a) Vel
b) S
c) Kpa
d) K
a) Infectious mononucleosis
b) Chronic granulomatous disease
c) Hodgkin’s disease
d) PCH
Which set of antibodies could you possibly find in a patient with no history of transfusion or
pregnancy?
The red cells of a donor have a phenotype of U-negative. What red cell antibody would not react
with these red cells?
a) Anti-M
b) Anti-S
c) Anti-P1
d) Anti-K
a) Duffy
b) Kidd
c) P
d) Kell
HTLA antibodies:
A multiple antibodiy problem was resolved by using enzymes. One of the antibody reactions was
eliminated after treatment. Which of the following antibodies was probably present?
a) Anti-c
b) Anti-I
c) Anti-Jka
d) Anti-Fya
a) Anti-Jsb
b) Anti-Kpb
c) Anti-k
d) All of the above
What is the purpose of including a reagent control when interpreting group AB, D-positive red
cells after testing with a low protein anti-D reagent?
a) Glycolipids
b) Glycoproteins
c) Oligosaccharides
d) Transferase enzymes
Given the ABO typing results, what conclusion can be drawn from these results?
Anti-A Anti-B A1 B
4+ 4+ 1+ 0
Using known source of reagent antisera to detect ABO antigens on patients red cells is known
as:
a) Rh typing
b) Reverse grouping
c) Direct antiglobulin test
d) Forward grouping
a) D-galactose
b) L-fucose
c) N-acetylgalactosamine
d) L-glucose
a) H
b) A
c) Se
d) B