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Galss fermenter ( Off-site sterilization autoclaves) BLBIO-5GJG

Glass fermenter （ Off-site sterilization autoclaves ， (Magnetic Drive  glass  tank)

BLBIO-5GC

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Glass fermenter (Off-site sterilization autoclaves) BLBIO-5GJ

Galss fermenter (sterilizing in situ.、Magnetic Drive  glass  tank) BLBIO-5GCA

5 liters  Glass fermentor( Automatic sterilization in situ)   BLBIO-5GJAV

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5 liters  Glass fermentor( Automatic sterilization in situ)   BLBIO-5GJA

Thank you for your trust in our company and giving us the opportunity to provide programs and
offer。We guarantee to provide you with high quality and satisfactory service。

As a leading fermentation technology, equipment supply and technical value-added services

supplier，our company has a wealth of experience in project and outstanding professionals.

Advanced technology, Services responsive and high cost-effective is our operation principle.

In this program document, We will strive to achieve the following key points：

 according to customer requirements, we carry on the preliminary design.

 clearing objectives of the project，submit the quotation；

The fermenter plant offered is comprising fermenter designed and built for a broad range of
modern fermentation applications.

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The system will be designed to meet all requirements of modern fermentation technology,
utilizes only high quality industrial grade components ensuring optimum performance and a high
level of safety in operation.

If this program can be accepted, We will do our best for your company to provide such as
Stabledand reliable production device and Systematic and Comprehensive Technical Training
Services

We await a good news with patience.

Sincerely yours,
Company Name: Shanghai BaiLun  Biotechnology Co., Ltd. 
Address: Jiu Ting Town, Songjiang District, Shanghai city, China, kangMing
Road, No. 8
Contact: 15821268711 ,   lian ming  Zhu        Email:zlm@163.com
Website:  http://www.blbio.com/en
 
Company Name: lianyungang bailun Biotechnology Co., Ltd. 
Address: XinPu Development Zone,Lianyungang City, Jiangsu Province, China ,
Zhujiang Road  head, East State Road 204
Contact:  +86 15821268711    http://www.fermentors.cn/en    email：blbio369@163.com

Project price list

Products and name of goods, quantity and price ＄（＄）

Serial name specification numb quotation Offer

er Price

5 liters glass
fermenter(Off-site
1 BLBIO-5GJG/5GC/5GJ 1 19000 12000
sterilization
autoclaves)
5 liters 
Glass fermentor(  BLBIO-
2 Automatic 5GCA/5GJA/5GJAV/5GCA 1 23000 15000
sterilization in V
situ) 

3 Air compressor 1 500 500

Dfermenerelivery time: after signing a contract in35 days.

The above price includes domestic transportation expenses already installation and
commissioning, personnel training costs.

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5
A All
rights of the scheme and its whole content reserved by BaiLun Anyone who reads or
knows the scheme is regarded as to abide by the secret provision and will not
disclose it to the third party directly or not. The light system is not
showed in the chart. It can be seen on the homepage of our company.

Delivery date: 35th working day after signing the contract.

The quotation above includes the domestic transportation fare, the expenses of installation &
debugging and stuff training.

Without mechanical sealing, magnetic force stirrer can be used during the
sterilization. It is suitable for generic microorganism culture specially for the
medicament microbe culture with long ferment cycle.
Mechanical stirrer with mechanical sealing is suitable for microbe culture in the ropy
medium (i.e. xanthan gum)
BLBIO-5GJG/5GC/5GJ auto fermentation tank
一、 auto fermentation tank
二、 System constitute:
No Item Description
1 Vessel system 5 liters total volume
1--4 liters working volume
Glass vessel with rounded bottom and double jacket for
temperature control
Lid
Stainless steel 316L
With sterile and pressure proof ports
14 x diameter 12mm and 2 x diameter 19mm
1 port for top drive
5 x 1-way connection piece, height adjustable harvest tube.
Drive
Direct drive from top, DC motor, 90 W, 30 – 1500 rpm
Agitation
Mechanical seal, shaft, 2 height adjustable six – blade stirrer,
removable baffle cage
Aeration
Auto cleavable sterile filter, check valve, connection piece,
aeration tube with ring sparger
Required air pressure approx, 1.5 bar
Ventilation
Reflux cooler, connection piece, auto cleavable sterile filter

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Temperature Circuit
Connected to glass double jackets, all four temperature circuits
connected by central supply and drain lines.
Probes
Autoclavable temperature probe Pt100 incl. connecting cable
Autoclavable pH-gel-electrode incl. connecting cable

Autoclavable pO2-probe incl. connecting cable

Autoclavable height adjustable antifoam-probe incl. connecting
cable
Sampling System
Autoclavable sampling system
Inoculum
Cup for silicone diaphragm
Measurement of Controls
Integrated in stainless steel trunking
Power supply
Main power supply for all electrical circuits, 1 x 230V, 50 Hz
Temperature Control
Including heating finger and cooling water valve in temperature
circuit. Temperature controller for heating and cooling,
temperature range 4-60C (depending on the available cooling
media)
Speed Setting
DC motor with frequency converter, rpm-control unit
pH Control
pH-control to activate the dosage of acid and base (pumps)
pO2 Control
DDC pO2-control to activate the final elements, Cascade
control vial stirrer speed and aeration valve
Antifoam Control
DDC antifoam - Control to activate the dosage to antifoam
chemicals
Dosage
Aeration Unit
Consists of pressure valve, a rotameter (50-500L/h) a
continuous solenoid valve (proportional valve), activated by
pO2 controller, a check valve.
Peristaltic Pump with variable speed

1 for media feed, reversible, speed adjustable peristaltic pump

including 2 meters silicone hose, hose clamp and GL45 screw
cap with sterile filter. Suitable for pO2 cascade control

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Peristaltic Pump with fixed speed
3 for acid, base and antifoam / harvesting fixed speed
peristaltic pumps including 2 meters silicone hose and hose
clamp. 3 x 250 ml auto cleavable glass bottle with sterile filter
and 1 x GL 45 screw cap with sterile filter.
2 Aeration system Include rotameter, aeration filter
3 Agitation system 5GJ/5GJG：
machine: infinite speed variation; rpm in the 7L germ
fermenter: 70~1200rpm±1%,The height of the stirring oar can
be adjusted,
5GC：
Magnetic Drive from bottom, without mechanical seal
The agitation system hanged up is driven by magnetic force.
There is no thimble at the bottom. The rotate speed is steady.
High-powered stirrer, antifoam stirrer, AC buncher, variable
speed control. All parts in the cylinder are set on the cover, so it is
convenient to maintain and clean. Standard six- bladed paddle used
in fermentation process can be adjusted in the height. The stirrer
which has many other styles can be used to meet the special
requirement of fermentation process. There is no possibility to leak
out the bacteria without mechanical sealing. AC buncher, actiyator.
The design which separates the motor from the cylinder makes the
weight of cylinder cut down, so it is convenient to operate and save a
large amount of manpower. The magnetic force system uses special
permanent magnetic material. The special process makes the
magnetism permanent. The highest rev will never be less than 980
rpm during the service life of the cylinder. It can meet the highest rev
even in the full bodied medium.
4 Temperature Interlayer electric calefaction, auto-control
control
5 Airflow control Manual control, Flow meter display
6 Antifoam control Automatically check foam, automatically add antifoam by peristaltic
pump.
7 Feeding control Control by peristaltic pump switch, automatically feeding and
computation.
8 pH control Automatically add acid, alkali by peristaltic pump and control pH
accurately.
9 DO control Control with rotating speed relatively.
10 BLBIO-B Biology Control parameter: temp, pH, DO, antifoam, stirrer rev, feeding;
process controller (Other parameters like glucose, amino acid, ethanol, oxygen, carbon
dioxide, electric balance system can be chosen to use.)
Control system BLBIO B Control System: on-site controller, the next crew for the
Siemens S7-200 series PLC control system, it is a mature, stable,

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universal type of PLC, to suit a variety of automation applications,
especially in manufacturing process control Application. Its modular,
easy to implement distributed configuration and easy to master and
so on, Siemens PLC control center has become a biological reaction
process control implementation is economical and advanced control
system, the system uses the touch screen display, on-site direct
operations (menu type), all English menu and interface; also with the
host computer for data transmission, data sampling and display tank,
the system view of data analysis, reading settings.
10-inch LCD touch-sensitive screen as the display screen to display
complete content-rich, user-friendly screen is simple, easy to operate.
And disturbing each other. The control system can adapt to different
range of sensors and actuators the output of the input signal with the
signal anti-jamming system.
Software: Siemens S7+ FORCE CONTORL
Data collection and control module: Siemens S200 PLC controller
and data collection and control module
1. Control
① Manual control: You can set the percentage of open valve or a
valve opening ② automatic control mode: You can select continuous
or PID PID control the switch ③ sequence control: the control of all
parameters can be pre-set at least 10 control section, ④ for automatic
segmentation remotely control: PC control ⑤ acceptable correlation
control: the dissolved oxygen can select speed, air flow rate, tank
pressure, control of feeding, etc.; pH can choose to control with acid
and alkali

2. Measurement function: can feeding, bubble enemy, such as

measurement of pH
3. XY-axis to chart real progress can be any expansion and
contraction of graphics display, to help the operator determine the
parameters of the interaction between the effects of the fermentation
process to quickly modify and adjust
4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) data storage format is compatible with EXCEL, EXCEL platform

can be to deal with
5. Controller self-protection function: to set the password, others can
not modify the fermentation parameters; and the controller is not lost

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due to power settings for each parameter
6. Can the process control parameters, each parameter can have 10
block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and
calibration
9. show the controller is really running against, the panel instructed
the state process system, process hardware, and motion status
indicator for understanding the various devices (such as peristaltic
pumps, circulating pumps, heating, mixing, etc.) work status, Fault
identification can be used as display
10. Each parameter of the transmission are independent modules,
without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls
can be adjusted
11 PC software BLBIO-Ver8.0 is a data recording and processing as one of the
fermentation of special packages, with the english operation
interface, data storage and backup, curve analysis, report generation
analysis, anomaly analysis, for the fermentation and production
process control and data processing bring a lot of convenience.
Main features:
1. Can record the fermentation time, temperature, pH, dissolved
oxygen, rotational speed, air flow, pressure, fill quantity, the amount
of foam enemy, acid dosage
2. Can be sent under the controlled variable settings and control
parameters, remote operation
3. Can be set sequence control
4. Can type off-line parameters, and records show
5. Trend curve, historical curve shows
can display 1-10 (any setting) curve;
- You can select a different start time and the time period
(8,24,48,72,96 and 144 hours);
- You can set the upper and lower curves show, in order to improve
display resolution;
- You can choose any of several different batches (1-10) curve also
shows that for comparative analysis;
- and Y-axis parallel to the secant by the mouse to drag the reading,
followed by curve data is activated, the fermentation time (X axis)
and the same time the data curve with the secant movement changes;
6. Report Printing
- can show significant 1-12 (any set) data, and print output;
- You can select a different start time and the time and can choose
different time intervals (1,5,15,30,60 minutes);

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- You can choose any of several different batches (any set) data, and
print output,
- To comparative analysis;
7. curve printing
- You can show the curve was printed by color printer;
EXCEL platform can also be carried out in data processing and
printing, is very convenient.

三、 Technical parameter:
Parameter
No Guideline Controller data processing
function
1 PH Displaying range: 0.00~14.00±0.01 pH value historical and trend
Auto-control range:2.00~12.00±0.05 curve analysis
Fermentation process control Acid, alkali dosage curve
(According to the fermentation time, auto- analysis
control pH fluent, at least10 parts) Batch report form analysis
Acid, alkali total dosage record
Keep and deliver data
2 DO 0-150±3％，Displaying precision: 0.1％ DO value historical and trend
It can be controlled by rev and feeding. curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control DO fluent, at least10 parts)

3 Antifoam value historical and

Antifoam Automatic PID control and alarm trend curve analysis
Fermentation process control Foam error state record
(According to the fermentation time, auto- antifoam total dosage record
control antifoam fluent, at least10 parts) Batch report form analysis
Keep and deliver data

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4 Automatic enactment and Rev value historical and trend
Rev control（50~1200rpm±5‰） curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control rev fluent, at least10 parts)

5 Temperature of cooling water: +5℃- Temperature value historical

Temperature 80℃±0.2℃ and trend curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control temperature fluent, at least10
parts)

6 PID automatic enactment and control Feeding value historical and

Feeding Fermentation process control trend curve analysis
(According to the fermentation time, auto- Feeding total dosage record
control feeding fluent, at least10 parts) Batch report form analysis
Keep and deliver data
7
Pressure Manual control, instrument display
8
Air flux Manual control, instrument display

四、 Material and brand of key components

No. Key component Material and brand Quantity

1 Vessel system 2
The cylinder designed by our company uses stainless
steel combined with glass partly. Stainless steel:Glass:
borosilicate glass. The framework is advanced.
2 Buncher High grade aluminum alloy 1

3 Speed regulator Panasonic, Japan

4 Solenoid valve Taiwan 2

5 Aeration filter 0.2μm, SARTORIUS, Germany 6

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6 Breathing filter 0.2μm, SARTORIUS, Germany 12

7 pH electrode Mettler-Toledo, Switzerland 3

8 DO electrode Mettler-Toledo, Switzerland 3

9 Peristaltic lange 6
pump

5 liters glass     fermentor/fermenter （ sterilizing in situ)  BLBIO-5GCA/5GJA/5GJAV

System constitute:
No Item Description
1 Vessel system Total volume: 5 litres, Working volume: 1-4 liters
Strainless steel cylinder combines with glass partly. It uses a
large area of highly refractory glass to observe material easily.
Magnetic force stirrer without axes sealing. There is no
possibility to leak out the bacteria. The view is spacious. The
agitation system is hanged up. There is no thimble at the bottom.
Ratio of diameter to high: 1:2
Sterilization method: AUTO sterilizing in situ. It can stir at same
time of sterilization. So it can sterilize the culture medium
thoroughly.
2 Aeration system Include rotameter, aeration filter
3 Agitation system 5GCA：
Magnetic Drive from bottom, without mechanical seal
The agitation system hanged up is driven by magnetic force.
There is no thimble at the bottom. The rotate speed is steady.
High-powered stirrer, antifoam stirrer, AC buncher, variable
speed control. All parts in the cylinder are set on the cover, so it is
convenient to maintain and clean. Standard six- bladed paddle used
in fermentation process can be adjusted in the height. The stirrer
which has many other styles can be used to meet the special
requirement of fermentation process. There is no possibility to leak
out the bacteria without mechanical sealing. AC buncher, actiyator.
The design which separates the motor from the cylinder makes the
weight of cylinder cut down, so it is convenient to operate and save a
large amount of manpower. The magnetic force system uses special
permanent magnetic material. The special process makes the
magnetism permanent. The highest rev will never be less than 980
rpm during the service life of the cylinder. It can meet the highest rev
even in the full bodied medium.

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5GJAV：

Mixing method: Direct Drive  Mechanical agitation  John Crane

mechanical seals servo motor
4 Temperature Interlayer electric calefaction, auto-control
control
5 Airflow control Manual control, Flow meter display
6 Antifoam control Automatically check foam, automatically add antifoam by peristaltic
pump.
7 Feeding control Control by peristaltic pump switch, automatically feeding and
computation.
8 pH control Automatically add acid, alkali by peristaltic pump and control pH
accurately.
9 DO control Control with rotating speed relatively.
10 BLBIO –B Control parameter: temp, pH, DO, antifoam, stirrer rev, feeding;
Biology process (Other parameters like glucose, amino acid, ethanol, oxygen, carbon
controller dioxide, electric balance system can be chosen to use.)
Control system BLBIO B Control System: on-site controller, the next crew for the
Siemens S7-200 series PLC control system, it is a mature, stable,
universal type of PLC, to suit a variety of automation applications,
especially in manufacturing process control Application. Its modular,
easy to implement distributed configuration and easy to master and
so on, Siemens PLC control center has become a biological reaction
process control implementation is economical and advanced control
system, the system uses the touch screen display, on-site direct
operations (menu type), all English menu and interface; also with the
host computer for data transmission, data sampling and display tank,
the system view of data analysis, reading settings.
10-inch LCD touch-sensitive screen as the display screen to display
complete content-rich, user-friendly screen is simple, easy to operate.
And disturbing each other. The control system can adapt to different
range of sensors and actuators the output of the input signal with the
signal anti-jamming system.
Software: Siemens S7+ FORCE CONTORL
Data collection and control module: Siemens S200 PLC controller
and data collection and control module
1. Control
① Manual control: You can set the percentage of open valve or a
valve opening ② automatic control mode: You can select continuous
or PID PID control the switch ③ sequence control: the control of all
parameters can be pre-set at least 10 control section, ④ for automatic
segmentation remotely control: PC control ⑤ acceptable correlation

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control: the dissolved oxygen can select speed, air flow rate, tank
pressure, control of feeding, etc.; pH can choose to control with acid
and alkali

2. Measurement function: can feeding, bubble enemy, such as

measurement of pH
3. XY-axis to chart real progress can be any expansion and
contraction of graphics display, to help the operator determine the
parameters of the interaction between the effects of the fermentation
process to quickly modify and adjust
4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) data storage format is compatible with EXCEL, EXCEL platform

can be to deal with
5. Controller self-protection function: to set the password, others can
not modify the fermentation parameters; and the controller is not lost
due to power settings for each parameter
6. Can the process control parameters, each parameter can have 10
block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and
calibration
9. show the controller is really running against, the panel instructed
the state process system, process hardware, and motion status
indicator for understanding the various devices (such as peristaltic
pumps, circulating pumps, heating, mixing, etc.) work status, Fault
identification can be used as display
10. Each parameter of the transmission are independent modules,
without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls
can be adjusted
11 PC software BLBIO-Ver8.0 is a data recording and processing as one of the
fermentation of special packages, with the Chinese operation
interface, data storage and backup, curve analysis, report generation
analysis, anomaly analysis, for the fermentation and production
process control and data processing bring a lot of convenience.
Main features:
1. Can record the fermentation time, temperature, pH, dissolved
oxygen, rotational speed, air flow, pressure, fill quantity, the amount

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 Convenient practical simple professional
of foam enemy, acid dosage
2. Can be sent under the controlled variable settings and control
parameters, remote operation
3. Can be set sequence control
4. Can type off-line parameters, and records show
5. Trend curve, historical curve shows
can display 1-10 (any setting) curve;
- You can select a different start time and the time period
(8,24,48,72,96 and 144 hours);
- You can set the upper and lower curves show, in order to improve
display resolution;
- You can choose any of several different batches (1-10) curve also
shows that for comparative analysis;
- and Y-axis parallel to the secant by the mouse to drag the reading,
followed by curve data is activated, the fermentation time (X axis)
and the same time the data curve with the secant movement changes;
6. Report Printing
- can show significant 1-12 (any set) data, and print output;
- You can select a different start time and the time and can choose
different time intervals (1,5,15,30,60 minutes);
- You can choose any of several different batches (any set) data, and
print output,
- To comparative analysis;
7. curve printing
- You can show the curve was printed by color printer;
EXCEL platform can also be carried out in data processing and
printing, is very convenient.

Technical parameter:
No Parameter Guideline Controller data processing
function
1 pH Displaying range: 0.00~14.00±0.01 pH value historical and trend
Auto-control range:2.00~12.00±0.05 curve analysis
Fermentation process control Acid, alkali dosage curve
(According to the fermentation time, auto- analysis
control pH fluent, at least10 parts) Batch report form analysis
Acid, alkali total dosage record
Keep and deliver data
2 DO 0-150±3％，Displaying precision: 0.1％ DO value historical and trend
It can be controlled by rev and feeding. curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control DO fluent, at least10 parts)

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3 Antifoam Automatic PID control and alarm Antifoam value historical and
Fermentation process control trend curve analysis
(According to the fermentation time, auto- Foam error state record
control antifoam fluent, at least10 parts) antifoam total dosage record
Batch report form analysis
Keep and deliver data
4 Rev Automatic enactment and Rev value historical and trend
control（50~1000rpm±5‰） curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control rev fluent, at least10 parts)
5 Temperature Temperature of cooling water: +5℃- Temperature value historical
65℃±0.2℃ and trend curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control temperature fluent, at least10
parts)
6 Feeding PID automatic enactment and control Feeding value historical and
Fermentation process control trend curve analysis
(According to the fermentation time, auto- Feeding total dosage record
control feeding fluent, at least10 parts) Batch report form analysis
Keep and deliver data
7 Pressure Manual control, instrument display
8 Air flux Manual control, instrument display

Material and brand of key components

No. Key component Material and brand
1 Vessel system The cylinder designed by our company uses stainless steel combined
with glass partly. Stainless steel:SUS316L. Glass: borosilicate
glass. The framework is advanced.
2 Buncher High grade aluminum alloy
3 Speed regulator Panasonic, Japan
4 Solenoid valve TAIWAN
5 Aeration filter 0.2μm, import filterable membrane
6 Breathing filter 0.2μm, SARTORIUS, Germany
7 pH electrode Mettler-Toledo, Switzerland
8 DO electrode Mettler-Toledo, Switzerland
9 Peristaltic pump Britain

 Working environment:
 Up to the internal &external environment required by fermentation

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 Up to the technic-required water supply. The temperature of cooling
water is at least 5℃ below the temperature of fermentation
 Power requirement: AC220/2KW
 Compressed air: Primarily-processed compressed air, relative
humidity:≤60%,ventilatory capacity is accorded to the technics.

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