JAST - Volume 18 - Issue 3 - Pages 657-668
JAST - Volume 18 - Issue 3 - Pages 657-668
JAST - Volume 18 - Issue 3 - Pages 657-668
18: 657-668
ABSTRACT
The date fruit is of high nutritional value and its chemical composition is unique in
providing energy, minerals, and vitamins for human. Date syrup, date concentrate and
liquid date sugar are among semi-finished liquid products produced from low quality
dates at industrial scale. In this research, some physicochemical properties of date syrup,
concentrate, and liquid sugar in comparison with sucrose solutions were studied and the
possibility of replacing sucrose with date liquid products was investigated. The results
showed that the main chemical component of all three date products was simple sugars of
fructose and glucose. Ash and protein contents of date liquid sugar were much lower than
date syrup. pH values of date liquid products were also significantly different.
Concentration of total phenolic and flavonoid compounds in date syrup was much greater
than date liquid sugar. Overall, processing of date syrup to date liquid sugar reduced its
nutritional quality while improving technological properties. It can be concluded that in
choosing date liquid products as raw materials in food formulation, for the products that
brown color of date liquid products does not affect the appearance of the final products,
usage of date syrup or date concentrate is proposed. However, in other foods where
brownish color of date syrup or date concentrate deteriorates sensory attributes of the
final products, date liquid sugar is suggested. Date liquid products are also good
candidates for high sugar products with sugar crystallization problem.
_____________________________________________________________________________
1
Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Islamic
Republic of Iran.
2
School of Biomedical Sciences, Graham Center for Agricultural Innovation and Functional Grains Center,
Charles Sturt University, Wagga Wagga, New South Wales, Australia.
∗
Corresponding author; e-mail: farahnak@shirazu.ac.ir
657
____________________________________________________________________ Farahnaky et al.
658
Study of Date Syrup, Concentrate, and Liquid Sugar ______________________________
Figure 1. Large scale process chart of production of date syrup, date concentrate, and date liquid
sugar from date.
6.25. Soxhlet method with hexane was used with a glass-combined electrode (AOAC,
to determine the fat content and total 1997). Density was determined using 50 mL
carbohydrate was calculated by subtracting Pycnometers. To verify possible changes in
the total percent values of other their volume due to thermal expansion, each
measurements from 100. Total soluble solids Pycnometer was calibrated with distilled
(ºBrix) was determined (AOAC, 1997) with water at 25°C. Pycnometers were filled with
a refractometer (Carl Zeiss, Germany). samples and placed in a thermostatic bath
(±0.1°C) set at 25°C for equilibrium. The
Pycnometers were then quickly weighed
Determination of pH and Density
using a four digits Sartorius-GE412 balance
(Germany) according to Cepeda and
The pH value was measured with a pH- Villaran (1999).
meter (pH-meter Basic 20, Iran) supplied
659
____________________________________________________________________ Farahnaky et al.
660
Study of Date Syrup, Concentrate, and Liquid Sugar ______________________________
661
____________________________________________________________________ Farahnaky et al.
first product obtained in the processing line), minimum pH values recorded for all date
date concentrate (the second product), and products were 4.62 and 3.33, respectively,
date liquid sugar (the third product) were while these values were 7.88 and 7.38 for
4.24, 3.77, and 3.31, respectively. This sucrose solutions. This can have practical
finding was similar to the report of Brasil et implications in using date products instead
al. (1995) on pH variation of guava juice of sucrose, i.e. high percentages of date
during processing. They reported that with syrup, date concentrate or date liquid sugar
increasing enzyme treatment time and can reduce the final pH of food formulations
reducing viscosity, and increasing Brix, the in comparison with sucrose solutions. A
pH of guava juice decreased significantly. general trend of increasing pH with
Decrease of pH can be attributed to either decreasing Brix in all three date products
removal of dispersed or insoluble materials and sucrose solution was also observed. For
of amphoteric nature or structural changes of example, the pH of date liquid sugars with
some acidic compounds of date juice during Brix values of 12, 35, 65 and 72 were 4.06.
enzymatic and physical treatments. 3.53, 3.39 and 3.33, respectively. Higher
concentrations of date processing products
caused greater pH reduction, which is due to
pH and Density at Different
concentrations the fact date syrup, concentrate, and liquid
sugar all add compounds to water that are
able to reduce pH. These could come from
The pH values of solutions of date syrup, organic acids present in date fruit or
concentrate, liquid sugar, and sucrose produced during processing. This trend was
solutions with a wide range of Brix values also seen for sucrose solutions.
are presented in Table 2. Overall, the pH The density of date syrup, concentrate,
values of all date liquid products were liquid sugar, and sucrose solutions at a wide
significantly lower than their corresponding range of Brix values are compared in Table
sucrose solutions. The maximum and 3. Overall, little but significant differences
Table 2. pH of date syrup, concentrate and liquid sugar compared with sucrose solution of 12, 35, 65,
and 72° Brix (total soluble solids).a
12 35 65 72
Date syrup 4.62 ± 0.01 A,b 4.46 ± 0.01 B,b 4.28 ± 0.02 C,b 4.24 ± 0.01 C,b
Date concentrate 4.26 ± 0.02 A,c 4.08 ± 0.01 B,c 3.90 ± 0.02 C,c 3.80 ± 0.01 D,c
Date liquid sugar 4.06 ± 0.02 A,d 3.53 ± 0.01 B,d 3.39 ± 0.04 C,d 3.33 ± 0.04 C,d
Sucrose solution 7.88 ± 0.04 A,a 7.84 ± 0.02 A,a 7.46 ± 0.03 B,a 7.38 ± 0.03 C,a
a
Values are mean±SD of three replicates. Different capital letters show significant difference in
each row and different small letters show significant difference in each column between the
treatments. All statistical analysis was performed at P< 0.05.
Table 3. Density (g cm-3) of date syrup, date concentrate and date liquid sugar compared with
sucrose solution of 12, 35, 65 and 72° Brix at 25°C.a
12 35 65 72
Date syrup 1.045 ± 0.011D,a 1.150 ± 0.074C,a 1.317 ± 0.024B,a 1.351 ± 0.009A,a
Date concentrate 1.044 ± 0.021 D,b 1.150 ± 0.025 C,ab 1.315 ± 0.105B,b 1.342 ± 0.067A,ab
Date liquid sugar 1.043 ± 0.014C,c 1.149 ± 0.003B,b 1.309 ± 0.011A,d 1.309 ± 0.020A,c
Sucrose solution 1.042 ± 0.023C,d 1.147 ± 0.017B,c 1.311 ± 0.033A,c 1.324 ± 1.850A,bc
a
Values are mean±SD of three replicates. Different capital letters show significant difference in
each row and different small letters show significant difference in each column between the
treatments. All statistical analysis was performed at P< 0.05.
662
Study of Date Syrup, Concentrate, and Liquid Sugar ______________________________
were found between densities of date syrup, Total Phenolic and Flavonoid Contents
date concentrate, date liquid sugar and
sucrose solutions with same Brix values. For Total phenolic compounds level of date
example, density of syrup, concentrate, and syrup was almost 10 times greater than that
liquid sugar at Brix 45 were 1.150, 1.150, of date liquid sugar and values of 453.04,
1.149 and 1.147 g cm-3, respectively. The 173.78 and 48.36 (mg GAE/100g sample)
densities of sucrose solutions were closer to were recorded for date syrup, date
date liquid sugar among the three date concentrate, and date liquid sugar,
products. By decreasing soluble solids respectively (Table 4). The highest and the
(Brix) the density of all four samples lowest amounts of total flavonoid were
decreased significantly, for example the found for date syrup and liquid sugar,
density of date concentrate at Brix values of respectively. Total flavonoid compounds
12, 35, 65 and 72 were 1.044, 1.150, 1.315 were 11.93, 5.02 and 3.31 mg (QE/100g
and 1.342 g cm-3, respectively. This is in line sample) for date syrup, concentrate, and
with the report of Zuritz et al. (2005) on liquid sugar, respectively. In a previous
clear grape juice at different soluble solid study, date syrup was found to be a good
concentrations and temperatures. source of phenolic compounds ranging from
39.56 to 88.27 (mg catechin 100 g-1 of
Antioxidant Activity: DPPH Radical syrup) (Abbès et al., 2013). The difference
Scavenging Activity in the results could be duo to date variety
and possibe variations in determination
A lower IC50 indicates better radical method (Al-Farsi and Lee, 2008). These
scavenging. According to Table 4, findings show that processing unit
antioxidant activity (IC50 of BHT/IC50 of operations used from date syrup to date
sample) of date syrup is greater than date liquid sugar reduce or remove the natural
concentrate and date liquid sugar. This functional compounds of date syrup.
indicates the possible removal or destruction
of antioxidant compounds during Sugar Content
processing, enzyme treatment, and
clarification or filtration. This finding is in Chromatograms of sugar analysis of the
agreement with those reported by Abbès et samples are presented in Figure 2 and sugar
al. (2013). contents are given in Table 4. Glucose
Table 4. Antioxidant activity, total phenolic and flavonoid content, glucose and fructose and
hydroxymethyl furfural of date syrup, date concentrate and date liquid sugar.a
HMF Antioxidant Phenol Flavonoid Glucose Fructose
(µg kg-1) (IC50 (mg of GAE 100 (mg QE 100 (g 100 (g 100
TBHQ/IC50 g-1sample) g-1 sample) g-1 sample) g-1 sample)
Sample)
Date 456.11 0.18 ± 0.03A 453.04 ± 0.50A 11.93 ± 0.52A 27.75 25.16
Syrup
Date 639.24 0.05 ± 0.01B 173.78 ± 0.18B 5.02 ± 0.65B 26.43 23.19
concentrate
Date liquid 787.99 0.02 ± 0.00B 48.36 ± 0.20 C 3.31 ± 0.17C 33.55 27.09
sugar
Sucrose 0.16 - - - - -
solution
a
Values are mean±SD of three replicates. Different capital letters show significant difference in each
column. All statistical analysis was performed at P< 0.05.
663
____________________________________________________________________ Farahnaky et al.
nRIU(x104)
nRIU(x104) 7.393
7.415 10 B
A 7.794
10 7.823
8
8
6
6
4
4
2
2
5 6 7 8 9 10 min
5 6 7 8 9 10 min
nRIU(x104)
7.411 C
10 7.816
5 6 7 8 9 10 min
Figure 2. HPLC chromatograms of sugars: (A) Date syrup; (B) Date concentrate, and (C) Date
liquid sugar. The peaks at about 7.4 and 7.8 min are for glucose and fructose, respectively.
664
Study of Date Syrup, Concentrate, and Liquid Sugar ______________________________
mAU
2.592 A 2.593 B
70
100
60
50 80
40 60
30
40
20
20
10
0 0
0 1 2 3 4 min 0 1 2 3 4 min
mAU mAU
2.592 C 10 D
120
8
100
6
80
4
60 2
40 0
20 -2 2.585
0 -4
0 1 2 3 4 min 0 1 2 3 4 min
Figure 3. A chromatogram of hydroxymethyl furfural by HPLC: (A) Date syrup, (B) Date
concentrate; (C) Date liquid sugar, and (D) Sucrose solution.
665
____________________________________________________________________ Farahnaky et al.
sugar could be positive in formulation of Shiraz Grand Industrial Estate Zone, Shiraz,
healthy foods. Iran. Cooperation of the productions staff of
Minoo Date Factory (Fars, Iran) is gratefully
acknowledged.
CONCLUSIONS
666
Study of Date Syrup, Concentrate, and Liquid Sugar ______________________________
667
____________________________________________________________________ Farahnaky et al.
ﺑﺮﺧﻲ وﻳﮋﮔﻲ ﻫﺎي ﻓﻴﺰﻳﻜﻮﺷﻴﻤﻴﺎﻳﻲ ﺷﻴﺮه ،ﻛﻨﺴﺘﺎﻧﺘﺮه و ﻗﻨﺪ ﻣﺎﻳﻊ ﺧﺮﻣﺎ در ﻣﻘﺎﻳﺴﻪ ﺑﺎ
ﻣﺤﻠﻮل ﻫﺎي ﺷﻜﺮ
ﭼﻜﻴﺪه
ﻣﻴﻮه ﺧﺮﻣﺎ داراي ارزش ﺗﻐﺬﻳﻪ اي ﺑﺎﻻﻳﻲ اﺳﺖ و ﺗﺮﻛﻴﺒﺎت ﺷﻴﻤﻴﺎﻳﻲ ﻣﻨﺤﺼﺮ ﺑﻔﺮدي را ﺑﺮاي ﺗﺎﻣﻴﻦ
اﻧﺮژي ،ﻣﻮاد ﻣﻌﺪﻧﻲ و وﻳﺘﺎﻣﻴﻦ ﻫﺎي ﻣﻮرد ﻧﻴﺎز اﻧﺴﺎن دارد .ﺷﻴﺮه ،ﻛﻨﺴﺘﺎﻧﺘﺮه و ﻗﻨﺪ ﻣﺎﻳﻊ ﺧﺮﻣﺎ ﻓﺮاورده ﻫﺎي
ﻣﺎﻳﻊ ﻧﻴﻤﻪ آﻣﺎده ﻳﻲ ﻫﺴﺘﻨﺪ ﻛﻪ از ﻓﺮاوري ﺧﺮﻣﺎ ﻫﺎي داراي ﻛﻴﻔﻴﺖ ﭘﺎﻳﻴﻦ در ﻣﻘﻴﺎس ﺻﻨﻌﺘﻲ ﺗﻮﻟﻴﺪ ﻣﻲ
ﺷﻮﻧﺪ .در اﻳﻦ ﺗﺤﻘﻴﻖ ﺑﺮﺧﻲ وﻳﮋﮔﻲ ﻫﺎي ﻓﻴﺰﻳﻜﻮﺷﻴﻤﺎﻳﻲ ﺷﻴﺮه ،ﻛﻨﺴﺘﺎﻧﺘﺮه و ﻗﻨﺪ ﻣﺎﻳﻊ ﺧﺮﻣﺎ در ﻣﻘﺎﻳﺴﻪ ﺑﺎ
ﻣﺤﻠﻮل ﻫﺎي ﺷﻜﺮ ﻣﻄﺎﻟﻌﻪ و اﻣﻜﺎن ﺟﺎﻳﮕﺰﻳﻨﻲ ﺷﻜﺮ ﺑﺎ ﻓﺮاورده ﻫﺎي ﻣﺎﻳﻊ ﺧﺮﻣﺎ ﺑﺮرﺳﻲ ﮔﺮدﻳﺪ .ﻧﺘﺎﻳﺞ ﻧﺸﺎن
داد ﻛﻪ ﺗﺮﻛﻴﺐ اﺻﻠﻲ ﻫﺮ ﺳﻪ ﻣﺤﺼﻮل ﺧﺮﻣﺎ را ﻗﻨﺪ ﻫﺎي ﺳﺎده ﻓﺮوﻛﺘﻮز و ﮔﻠﻮﻛﺰ ﺗﺸﻜﻴﻞ ﻣﻲ دﻫﻨﺪ.
ﺧﺎﻛﺴﺘﺮ و ﭘﺮوﺗﺌﻴﻦ ﻗﻨﺪ ﺷﻴﺮه ﺧﺮﻣﺎ ﺑﺴﻴﺎر ﭘﺎﻳﻴﻦ ﺗﺮ از ﻗﻨﺪ ﺷﻴﺮه ﺧﺮﻣﺎ ﺑﻮد .ﻣﻘﺎدﻳﺮ pHﻓﺮاورده ﻫﺎي ﻣﺎﻳﻊ
ﺧﺮﻣﺎ اﺧﺘﻼف ﻣﻌﻨﻲ دار ﻧﺸﺎن دادﻧﺪ .ﻏﻠﻈﺖ ﺗﺮﻛﻴﺒﺎت ﻓﻨﻠﻲ ﻛﻞ و ﻓﻼووﻧﻮﺋﻴﺪ ﺷﻴﺮه ﺧﺮﻣﺎ ﺑﻴﺸﺘﺮ از ﻗﻨﺪ ﺷﻴﺮه
ﺧﺮﻣﺎ ﺑﻮد .ﺑﺎ ﻓﺮاوري ﺷﻴﺮه ﺧﺮﻣﺎ ﺑﻪ ﻗﻨﺪ ﺷﻴﺮه ﺧﺮﻣﺎ ،ﻛﻴﻔﻴﺖ ﺗﻐﺬﻳﻪ اي ﻛﺎﻫﺶ و ﻛﻴﻔﻴﺖ ﺗﻜﻨﻮﻟﻮژﻳﻜﻲ ﺑﻬﺒﻮد
ﻳﺎﻓﺖ .در اﻧﺘﺨﺎب ﻓﺮاورده ﻫﺎي ﻣﺎﻳﻊ ﺧﺮﻣﺎ ﺑﺮاي ﻓﺮﻣﻮﻻﺳﻴﻮن ﻣﻮاد ﻏﺬاﻳﻲ ،ﺑﺮاي ﻣﺤﺼﻮﻻﺗﻲ ﻛﻪ رﻧﮓ ﻗﻬﻮه
اي ﻓﺮاورده ﻫﺎي ﻣﺎﻳﻊ ﺧﺮﻣﺎ ﺑﺮ ﻛﻴﻔﻴﺖ ﻇﺎﻫﺮي ﻏﺬاﻫﺎ اﺛﺮ ﻧﺎﻣﻄﻠﻮب ﻧﻤﻲ ﮔﺬارد ،ﺷﻴﺮه ﺧﺮﻣﺎ و ﻛﻨﺴﺘﺎﻧﺘﺮه
ﺧﺮﻣﺎ ﭘﻴﺸﻨﻬﺎد ﻣﻲ ﮔﺮدد .در ﺣﺎﻟﻲ ﻛﻪ در ﺳﺎﻳﺮ ﻣﻮاد ﻏﺬاﻳﻲ ﻛﻪ رﻧﮓ ﻗﻬﻮه اي ﺷﻴﺮه ﺧﺮﻣﺎ و ﻛﻨﺴﺘﺎﻧﺘﺮه ﺧﺮﻣﺎ
اﺛﺮات ﻧﺎﻣﻄﻠﻮب دارد ،اﺳﺘﻔﺎده از ﻗﻨﺪ ﺷﻴﺮه ﺧﺮﻣﺎ ﻣﻨﺎﺳﺐ ﺗﺮ اﺳﺖ .ﻫﻤﭽﻨﻴﻦ ﻓﺮاورده ﻫﺎي ﻣﺎﻳﻊ ﺧﺮﻣﺎ ،ﺷﻴﺮﻳﻦ
ﻛﻨﻨﺪه ﻣﻨﺎﺳﺒﻲ ﺑﺮاي ﻣﻮاد ﻏﺬاﻳﻲ داراي ﻣﻴﺰان ﺑﺎﻻي ﻗﻨﺪ ﻛﻪ ﺑﺎ ﻣﺸﻜﻞ ﻛﺮﻳﺴﺘﺎﻟﻴﺰه ﺷﺪن ﻗﻨﺪ ﻣﻮاﺟﻪ ﻫﺴﺘﻨﺪ
ﻣﻲ ﺑﺎﺷﻨﺪ.
668