Scribd Logo
Upload

Welcome to Scribd!

  • 10 views

    HPLC L 1

    Uploaded by

    PRAVINBHAI CHAUDHARY

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    HPLC L 1

    Uploaded by

    PRAVINBHAI CHAUDHARY
    10 views2 pages

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    10 views2 pages

    HPLC L 1

    Uploaded by

    PRAVINBHAI CHAUDHARY

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    High Performance/Pressure Liquid Chromatography (HPLC)

    Introduction

    Mobile phase: Liquid

    Stationary Phase: Liquid/Solid

    So if mobile phase is liquid and stationary phase is also liquid than it is known as
    Liquid-Liquid Chromatography (LLC).

    LLC is also known partition chromatography.

    So if mobile phase is liquid and stationary phase is also solid than it is known as
    Liquid-Solid Chromatography (LSC).

    LSC is also known adsorption chromatography.

    In HPLC, Both the phenomenon will use such as HPLC can work under partition as
    well adsorption phenomenon.

    In 1969, Kirkland and Huber developed chromatography which uses high


    pressure. They separated nucleic acids components.

    Important Characteristics of HPLC

    1. High resolving power and speedy separation


    2. Accurate quantitative measurement
    3. Continuous monitoring of the column effluent
    4. Repetitive and reproducible analysis using the same column.
    5. HPLC can able to separate macromolecules and ionic species, labile natural
    compounds, in a very little amount high molecular weight compounds
    6. It can determine multiple components in a single analysis.
    7. Separated components can be easily collected from the mobile phase for
    further characterization.
    8. A variety of solvents and column packings are available in HPLC, Sample
    recovery is easy.
    9. Analysis is completed in a few minutes with excellent precision and
    accuracy. >99%
    10. It provides automatic analytical procedure and data handling.

    Comparison of HPLC and Gas Liquid Chromatography (GLC).

    1. It is not limited to volatile and thermally stable substances. It


    accommodates thermally unstable, non volatile compounds.
    2. The choice of mobile phase and stationary phase are wider compare to the
    GLC.
    3. GLC is better from the point of view of speed and simplicity of equipment.

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy