Scribd
Upload
30 views

GC-MS Tecnique: Samples That Can Be Identified Using GC-MS Technique

GC-MS is a technique that combines gas chromatography and mass spectrometry. It is well-suited for separating and identifying small volatile molecules like fatty acids, amino acids, and organic acids in biofluids. GC separates components which are then analyzed by MS, allowing for more accurate identification than either technique alone. Common applications include drug testing and identifying metabolites related to inherited diseases.

Uploaded by

Hajra Aamir
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
30 views

GC-MS Tecnique: Samples That Can Be Identified Using GC-MS Technique

GC-MS is a technique that combines gas chromatography and mass spectrometry. It is well-suited for separating and identifying small volatile molecules like fatty acids, amino acids, and organic acids in biofluids. GC separates components which are then analyzed by MS, allowing for more accurate identification than either technique alone. Common applications include drug testing and identifying metabolites related to inherited diseases.

Uploaded by

Hajra Aamir
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 2

GC-MS Tecnique

Samples that can be identified using GC-MS Technique


GC-MS is a mature and established analytic technique with many applications in the
detection and measurement of endogenous metabolites and environmental exposure-related
metabolites in human biofluids and tissue extracts. The technique is ultimately well suited for the
separation of volatile chemical species, and the use of chemical derivatization procedures to
improve chemical volatility has been successful at extending the potential molecular coverage of
GC-MS to include a greater extent of human metabolites including small polar species that are
otherwise challenging to retain and separate by other hyphenated methods. GC-MS is therefore
commonly used for the identification of key small molecules such as fatty acids, amino acids,
and organic acids in biofluids, particularly in urine and serum or plasma. Thus, GC-MS has held
a central place in providing diagnostic information for many inherited diseases, including
numerous metabolic disorders.
One of the most common applications of GC-MS is drug testing for clinical or forensic purposes.
Many drugs have relatively low molecular weight and nonpolar and/or volatile properties,
making these compounds particularly suitable for analysis by GC.

Working
GC-MS is a hyphenated technique that combines the two methods of gas chromatography (GC)
and mass spectrometry (MS). As discussed above, GC uses a capillary column to separate
constituents in the sample by forcing a mobile phase containing the sample mixture through the
length of the column containing the stationary phase.

Each constituent in the sample will have a different affinity for the stationary phase and will,
therefore, elute from the column at different times known as the retention time. Each component
of the molecule that gets eluted at different times from the column is captured by the mass
spectrometer downstream, which is then ionized, subjected to an electromagnetic field and gets
deflected to a detector.

The intensity of each charged fragment and non-fragmented component is calculated as a


function of the mass to charge ratio. By coupling these two instruments together, it allows for
more accurate identification than either instrument used independently.

The disadvantage of using mass spectrometry on its own is that it relies on the purity of the
sample and that there is the possibility of two different molecular fragments sharing a similar
ionization pattern.
In contrast, the disadvantage of using GC on its own is that typically it cannot differentiate
between multiple molecules that have the same retention time and therefore elute at the same
time.

Hence, combining the two instruments reduces the possibility of error and increases the accuracy
of identifying the molecule of interest in the sample. Therefore, if a mass spectrum identifies a
molecule that has a characteristic retention time in the GC, it increases confidence that the two
techniques combined are identifying the constituent of interest in the sample.

Specifications

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy