Histologie Evaluation of New Attachment Apparatus

Formation in Humans
Part I
Gerald M. Bowers,* Brian Chadroff,f Robert Carnevale,t
James Mellonig,§ Rüssel Corio, | Jane Emerson,* Mark Stevens,H
and Elaine Romberg#

Part i of this three-part human study evaluated the formation of a new attachment
apparatus (bone, cementum, and periodontal ligament) on pathologically exposed root
surfaces in an open and closed environment. The most apical level of calculus on the root
served as a histologie reference point to measure regeneration on root surfaces exposed to
the oral environment. Attempts were made to initiate the formation of a new attachment
apparatus by flap curettage, root planing, coronectomy, and submersion of vital roots
beneath the mucosa. Nonsubmerged defects were treated by the same surgical technique
and served as controls. Biopsies were obtained at 6 months and regeneration was evaluated
histometrically by two investigators who were unaware of the treatment performed. Data
from 9 patients with 25 submerged and 22 nonsubmerged defects were submitted for
statistical analysis. Results indicate that a new attachment apparatus did not form in any of
the 22 nonsubmerged teeth; a new attachment apparatus did form in a submerged environ-
ment (0.75mm); significantly more new attachment apparatus (P < 0.05), new cementum
( < 0.01 ), new connective tissue ( < 0.05), and new bone ( < 0.02) formed in submerged
defects; new cementum was cellular in nature and formed equally well on old cementum
and dentin. Greater percent positive regeneration of the attachment apparatus and all
component tissues occurred in submerged defects and no extensive root résorption, ankylosis,
or pulp death was observed on submerged or nonsubmerged roots. (Journal of Periodontol-
ogy, 1989;60:664-674)

The regeneration of a new attachment apparatus flora than humans may produce misleading results.
(bone, cementum, and a functional periodontal liga- Even when the optimal primate model system reported
ment) after the natural attachment has been destroyed by Caton and Kowalski1 was used, completely divergent
by disease or traumatic injury is one of the most chal- findings were reported by Caton and coworkers2"4 and
lenging problems in dentistry. New attachment studies Mellonig.5 Caton was unable to promote new attach-
have been conducted in both animals and humans. ment in primates with or without grafting materials,
Unfortunately, it is not always possible to extrapolate whereas Mellonig demonstrated new attachment in
the results of new attachment studies in animals to grafted sites and in a significantly fewer number of
humans. Evaluation of new attachment in artificially- nongrafted defects. Animal studies will continue to
created defects in animals with a different bacterial provide useful information, but proof of formation of
a new attachment apparatus must come from histologie
evidence in humans. While evidence of the formation
*
Department of Periodontics, Baltimore College of Dental Sur- of a new attachment apparatus has been reported in
gery, University of Maryland, Baltimore, MD.
t Private practice, Rego Park, NY. humans,6 some question existing information on the
% Private practice, New London, CT. basis of inadequate documentation of denuded root
§ Department of Periodontics, School of Dentistry, University of surfaces,7"9 poor histologie technique,8 or inaccurate
Texas, San Antonio, TX; previously, Naval Dental Center, Periodon- reference points for measurement,7"9 and contend that
tics Department, Bethesda, MD. such regeneration is not feasible in areas previously
I Department of Oral Pathology, Georgetown University, School
of Dentistry, Washington, DC. exposed by Periodontitis. Others have also questioned
H Department of Removable Prosthetics, Baltimore College of the value of graft materials and whether or not a graft
Dental Surgery, University of Maryland, Baltimore, MD. contributes to new attachment.10 It is apparent that
#Department of Educational and Instructional Resources. several important questions need to be answered re-
664
Volume 60
Number 12 Histological Evaluation ofNew Periodontal Attachment, I 665

garding the formation of a new attachment apparatus. consent. This project was approved by the Human
In this three-part human study, regeneration of intra- Volunteers Committee, University of Maryland.
bony defects was compared in submerged and nonsub-
merged environments, with and without grafting ma- Presurgical Therapy
terials. In each of the three parts, the most apical level Treatment plans were developed and patients "re-
of calculus on the root served as the histologie reference ceived periodontal and restorative care as necessary to
point to measure regeneration.7 In Part I, attempts were restore their mouth to a state of health. Calculus was
made to regenerate intrabony defects by: 1 ) flap curet- not removed from the teeth included in the study.
tage, crown removal and submersion of the vital root
beneath the mucosa and 2) flap curettage alone. In Part Surgical Therapy
II, regeneration was compared in submerged intrabony All surgical procedures and clinical measurements
defects treated with and without decalcified freeze- were performed by the same investigator (GMB). On
dried bone allograft (DFDBA). Part HI evaluated the teeth randomly selected for vital root submersion (co-
potential for regeneration of intrabony defects on non- ronectomy), a mucoperiosteal flap was reflected on the
submerged teeth with and without the use of DFDBA. facial surface and a gingivoplasty was performed on the
In a preliminary report of this project, it was sug- lingual or palatal surface. Vertical incisions were made
gested that a new attachment apparatus may form after on the facial surface to permit coronal positioning of
flap curettage and root planing of intrabony defects if the flap. A small hole was placed in the crown of each
the vital roots are submerged beneath the mucosa.11 In tooth and suture material was passed through the hole
nonsubmerged teeth, using the same surgical technique, as a safeguard against aspiration of the crown once it
a long junctional epithelium formed over the patholog- was severed from the root. Crowns were amputated at
ically exposed root surfaces. This paper will present the the highest level of the alveolar crest with a #701 cross-
final results of Part I and attempt to answer the follow- cut fissure bur in a high speed handpiece with a sterile
ing questions: following flap curettage in intrabony water spray to prevent soft and hard tissue damage.
defects and root planing of pathologically exposed root Sharp edges of the roots were rounded with a finishing
surfaces bur. Granulation tissue was removed from all defects
1. Is it possible to regenerate a new attachment ap- without instrumenting the root surface. Once the gran-
paratus on nonsubmerged roots? ulation tissue was removed, a 1/4 round bur was used
2. Will a new attachment apparatus regenerate on to notch the root at the base of the calculus in order to
submerged vital roots? delineate exposed from nonexposed root surfaces.7 A
3. Are there significant differences in the amount of fiber optic light source was used to illuminate the defect
attachment apparatus and component tissues that will and the adjacent root surface and magnifying loops
form on submerged and nonsubmerged roots? were used to aid in locating the base of calculus. Root
4. Will the attachment apparatus and component planing was accomplished from the calculus reference
tissues form more frequently on submerged or nonsub- notch coronally by hand instrumentation and finishing
merged roots? burs. Special care was taken to preserve the position of
5. Will extensive root résorption, ankylosis, or pulp the notch during root planing. An ultrasonic instru-
death occur on submerged or nonsubmerged roots fol- ment* was used for final debridement of the defect but
lowing flap curettage and root planing? no attempt was made to contact the root surface apical
to the calculus reference notch. The bony defects were
classified as to the number of walls, and the depth of
MATERIALS AND METHODS each defect was measured with a calibrated periodontal
probe from the alveolar crest to the calculus reference
Patient Selection notch (groove) and from the notch to the depth of the
Thirteen volunteers were solicited from patients who defect (Fig. 1). In nonsubmerged teeth, the distance
had two or more maxillary or mandibular premolars, from the cemento-enamel junction to the alveolar crest
was also measured. The bony walls of the defects were
cuspids, or incisors recommended for extraction by the
Oral Diagnosis Department. The basis for extraction penetrated in numerous places with a 1/4 round bur
was advanced periodontal disease, excessive mobility, (intramarrow penetration) to enhance rapid prolifera-
malpositioned teeth, prosthetic considerations, esthet- tion of blood vessels into the defect. In order to obtain
ics, and patient request." Teeth chosen for the study soft tissue closure over the submerged roots, the facial
demonstrated advanced bone loss, deep pockets, and flap was coronally positioned and sutured over the
associated interproximal intrabony defects with radio- gingivoplasty wound. Vertical incisions were also su-
graphically visible calculus on the root surface. All teeth tured. The wound edges around the nonsubmerged
were vital and asymptomatic. Volunteers were system- teeth were tightly adapted with vertical mattress sutures.
ically healthy and instructed both orally and in writing
as to the nature of the project to obtain an informed *
Cavitron, Dentsply Corporation, York, PA.
 J. Periodontol.
666 Bowers, Chadroff, Carnevale, Mellonig, Corlo, Emerson, Stevens, Romberg December 1989

that cementogenesis has peaked at 3 months;12"14 how-

ever, 6 months was selected in order to compensate for
individual patient differences in wound healing. The
coronal portions of the submerged roots were exposed
to establish orientation, but interproximal tissue was
left undisturbed. Mucoperiosteal flaps were reflected
and the roots were sectioned with a #700 L cross-cut
fissure bur in a high speed handpiece with sterile water
spray. A small interproximal wedge of tissue and a
section of root approximately 5 mm wide, 7 mm long
and 5 mm thick was removed.11 Biopsies were notched
on the crown or root stump parallel to the desired plane
of sectioning to aid in proper alignment at the time of
embedding. Biopsies were then placed in buffered 10%
formalin. Remaining root fragments were removed and
a ceramic material* was placed into the biopsy and
extraction sites to maintain the original shape and in
AC- Notch at alveolar crest
some instances to augment the existing ridge. The facial
Groove- Notch at base of calculus
and palatal tissues were then repositioned and sutured
BD- Base of intrabony defect
over the sockets. Patients were seen in 7 days to remove
CEJ- Cementoenamel junction
the sutures. After the ridges had healed, the patients
Figure 1. Clinical Measurements. were referred to the Prosthetic Department for the
fabrication of permanent prosthetic appliances.
An adhesive material was placed over the surgical sites
of nonsubmerged teeth and the area was covered with
a periodontal dressing. If a temporary denture was Histologie Technique and Evaluation
placed at the time of surgery, it was relieved over the Specimens were processed at the Armed Forces In-
submerged roots. A resilient denture material was used stitute of Pathology and the University of Maryland
to reline the denture in 3 to 4 weeks. All patients were School of Dentistry. Biopsies were decalcified in EDTA,
given Tetracycline, 250mg, 4 times a day for 14 days embedded in paraffin, and serially sectioned at 7 µ .
while plaque control was compromised. A mild anal- Five submerged and 5 nonsubmerged specimens were
gesic such as Ibuprofen, 400mg, was also prescribed. embedded in plastic. Every ninth section was stained
All defects and root surfaces were treated identically with Masson's trichrome or Goldner's stain and every
except the nonsubmerged roots were notched at the tenth section with hematoxylin and eosin. A blind
highest level of the alveolar crest as well as at the base histologie evaluation of all biopsies was conducted by
of calculus. an oral pathologist (RC) and a periodontist (JM), to
determine the measurements shown in Figures 2 and
Postoperative Maintenance 3. It should be noted that the most coronal aspect of
Sutures were removed in 7 days and the sites were submerged roots served as reference point A, since the
debrided with saline. Nonsubmerged teeth were lightly roots were amputated at the highest level of the alveolar
debrided and polished. Patients were seen on a weekly crest at the time of surgery. Measurements were made
basis for the first month, every 2 weeks for the next 2 simultaneously using a dual attachment binocular mi-
months, and monthly for the 3 succeeding months for croscope, with a micrometer reticle.§ All serial sections
light debridement, 0.4% stannous fluoride application, with readily identifiable reference points and suitable
and reinforcement of plaque control when indicated. hard and soft tissue relationships were measured by the
Temporary appliances were evaluated at maintenance examiners. Magnification was altered to verify specific
visits and relined when indicated. Color transparencies tissue types and relationships but all measurements
were taken throughout the course of treatment. Radio- were made at 35 X. Examiners agreed on each meas-
graphs were retaken at the end of the 6-month period. urement before it was recorded. If there was disagree-
ment between examiners, both measurements were in-
Obtaining the Biopsy Specimens cluded and averaged. A mean score was determined for
At the end of 6
months, a second surgical procedure each measurement shown in Figures 2 and 3. In sub-
was performed to remove the submerged roots and
nonsubmerged teeth en bloc." Most studies indicate *
Synthograft, Johnson & Johnson Corporation, New Brunswick,
NJ.
§
Stomahesive, E. R. Squibb & Sons, Princeton, NJ. Micrometer Reticle, American Optical Corporation, Buffalo, NY.
Volume 60
Number 12 Histológica! Evaluation ofNew Periodontal A ttachment, I 667
merged sites, if there was evidence of epithelial "break-
through" (presence of a junctional epithelium), data
were collected but not included in this study. This was
necessary to insure that regeneration was evaluated in
a closed environment free of influence of junctional
epithelium and oral microflora. The examiners also
noted evidence of extensive root résorption, ankylosis,
pulp death, type and location of cementum formation
(over old cementum, dentin, or both) and orientation
of the periodontal ligament fibers (parallel, perpendic-
ular or both).
Number of Patients and Defects
During the 4 years of the project, two patients with-
drew from the study because of work requirements.
One submerged defect was not evaluated due to the
lack of a definitive reference notch. All submerged and
nonsubmerged defects were lost in one patient due to
Figure 2. Histological measurements on nonsubmerged defects technician error in sectioning. Three submerged defects
NAA New attachment apparatus formation demonstrated epithelial breakthrough and were ex-
NC New cementum -

cluded from the study. A total of 25 submerged and 22

New bone
-

AC Alveolar crest
-
nonsubmerged defects in 9 patients were submitted for
CT Width of connective tissue attachment
-
statistical analysis (Table 1).
JE Junctional epithelium
-

A Notch at clinical location of alveolar crest Statistical

Analysis
-

Notch at base of calculus

-

D Dentin
-

Repeated Measures General Linear Model Analyses

OC Old cementum
-

of Variance was used to test for significant differences

PL Periodontal ligament in amount of new attachment apparatus and compo-
-

BP Bone particle nent tissues that formed in experimental and control

-

sites. Serial sections of each defect were evaluated for

positive regeneration of a new attachment apparatus
8evered Root
A Table 1
Histological Measurements in mm: Comparison of Flap Curettage
in 25 Submerged and 22 Nonsubmerged Teeth, 9 Patients

Measurement
Submerged Nonsubmerged F
„

NAA 0.75±0.86f 0.00 ± 0.00 7.87 .05

NC 1.14 ±0.89 0.01 ±0.04 13.97 .01
CT 1.49 ±1.09 0.01 ±0.11 6.07 .05
NC and CT 2.51 ±0.80 0.02 ±0.14 78.57 .0001
NB 0.96 ± 0.90 0.02 ±0.12 10.00 .05
B- 2.58 ± 0.74 2.26 ± 0.64 1.16 .33
B-AC 0.99 ± 0.89 0.11 ±0.28 5.05 .07
B-JE 0.00 ±0.00
A-AC -1.46 ±0.93 -2.15 ± 1.08 3.21 .13
— —
—

AC-JE -0.30 ±1.39

— — —

Figure 3. Histological measurements on submerged defects NAA New attachment apparatus.

=

NAA New attachment apparatus formation NC New cementum.

=

NC New cementum -

CT Width of new connective tissue.

=

NB New bone
-

NC and CT New cementum plus new connective tissue attach-

=

AC Alveolar crest
-

ment.
CT Width of connective tissue attachment
-

NB =New bone.
A Root severed at highest level of alveolar crest
-
=
Reference notch at base of calculus.
Groove at base of calculus
-

A =
Reference notch at alveolar crest (nonsubmerged); severed
D Dentin
-

root surface at alveolar crest (submerged).

OC Old cementum
-

JE Most apical level of junctional epithelium.

=

PL Periodontal ligament
-

AC Microscopic location of alveolar crest,

=

BP Bone particle
-

t mean ± standard deviation (mm).

-
 J. Periodontol.
668 Bowers, Chadroff, Carnevale, Mellonig, Corio, Emerson, Stevens, Bömberg December 1989

and component tissue formation. The percentage of

positive regeneration for each technique was reported.
The most frequent location of new cementum forma-
tion, and the most common orientation of the peri-
odontal ligament fibers were also reported for each
technique.

RESULTS

Is It Possible to Regenerate a New Attachment Appa-

ratus on Nonsubmerged Roots?
The formation of a new attachment apparatus was
not observed in any of the 22 nonsubmerged teeth
treated by flap curettage and root planing (Table 1,
Figs. 4 and 5). On occasion, a small amount of new
cementum (xO.Ol mm), new connective tissue (xO.Ol
mm), or new bone formation (x0.02 mm) was observed
coronal to reference notch (Figs. 6 and 7). In no
instance, however, did all three tissues regenerate si-
multaneously to form a new attachment apparatus. In
nonsubmerged defects, the junctional epithelium was
usually located at or slightly below the alveolar crest
and the calculus reference notch (B-AC xO. 11 and =
Figure 5. Higher magnification of Figure 1 (region of arrow) dem-
AC-JE =
x-0.30). onstrating junctional epithelium/connective tissue/cementum inter-
face. (H&E, original magnification, 40x)

Figure 4. Representative section of nonsubmerged defect after flap

curettage and root planing. Note that location of the junctional epithe-
lium (JE) is apical to the calculus reference notch (B) and the alveolar Figure 6. Nonsubmerged defect demonstrating new cementum for-
crest. (H&E, original magnification, 4x). Arrow denotes junctional mation (arrow) at base of calculus notch (B). (H&E, original magni-
epithelium/cementum/connective tissue interface shown at higher fication, 4x). Higher magnification of region of arrow is shown in
magnification in Figure 2. Figure 4.
 Volume 60
Number 12 Histological Evaluation ofNew Periodontal Attachment, I 669

Will a New Attachment Apparatus Regenerate on Sub- Pathologically exposed root surfaces were most often
merged Vital Roots? covered by a combination of cementum and connective
A new attachment apparatus did form on pathologi- tissue (x2.51 mm) (Fig. 10).
New cementum was usually cellular in nature, varied
cally exposed root surfaces in a submerged environment in thickness from section to section, and was often
(x0.75mm) (Table 1, Figs. 8, 9, and 10). Complete
regeneration of the attachment apparatus, B- (x2.58 separated from the root surface. This separation was
considered artifact and related to acid décalcification
mm), was limited by the amount of bone and cemen-
tum formation (x0.96mm and 1.14mm respectively). and paraffin embedding, since the separation was not
observed in the 10 specimens which were decalcified in
EDTA and embedded in plastic. In submerged defects,
new cementum most frequently formed over both old
cementum and dentin within the same defect (Table 2,
Fig. 9). Periodontal ligament fibers were embedded in
cementum and bone and were most frequently oriented
parallel to the root (Table 3, Fig. 11).
Are There Significant Differences in the Amount of
Attachment Apparatus and Component Tissues That
Will Form on Submerged and Nonsubmerged Roots?
Data in Table 1 reveal that
significantly more attach-
ment .05), cementum ( < .01), con-
apparatus ( <
nective tissue (P .05), cementum plus connective
<
tissue (P < .0001), and bone (P < .05) formed in
Figure 7. Higher magnification ofcalculus notch (B) and new cemen- submerged defects than nonsubmerged defects. There
tum (NC) formation shown in Figure 3. New attachment apparatus was a difference in the mean location of the alveolar
formation was not evidenced coronal to calculus reference notch. crest from the calculus reference notch in submerged
(H&E, original magnification, 40x)

Figure 9. Higher magnification of calculus notch (B) in Figure 5

Figure 8. Submerged defect demonstrating the formation of new demonstrating the formation of new cellular cementum (NC), new
attachment apparatus from calculus reference notch (B) to arrow. New bone (NB) and new periodontal ligament (PL). Note parallel arrange-
cementum formation was observed at approximately the same level ment of periodontal ligament fibers and incorporation of Sharpey's
as new bone formation (Masson's trichrome, 4x). Higher magni- fibers in bone and cementum. Also note that new cementum formed
fication of calculus notch and region of arrow are shown in Figures over old cementum and dentin. Space between new cementum and
6 and 7. root is artifact. (H&E, original magnification, 40x)
 J. Periodontol.
670 Bowers, Chadroff, Carnevale, Mellonig, Corio, Emerson, Stevens, Bömberg December 1989

Will the Attachment Apparatus and Component Tissues

Form More Frequently on Submerged or Nonsub-
merged Roots?
Positive regeneration of the attachment apparatus,
cementum, connective tissue, cementum plus connec-
tive tissue, and bone occurred more frequently on
submerged roots than on nonsubmerged roots (Table
4).
Will Extensive Root Resorption, Ankylosis, or Pulp
Death Occur on Submerged or Nonsubmerged Root
Following Flap Curettage and Root Planing?
No extensive root résorption, ankylosis, or pulp death
was observed on submerged or nonsubmerged roots.
Surface résorption of old cementum and dentin was a
common finding on submerged roots and appeared to
precede cementogenesis (Fig. 12).
There significant difference between mean
was no
histological distances of submerged and nonsubmerged
reference notches B- (Table 1). Likewise, there was
no significant difference in probing measurements be-
tween alveolar crest and base of calculus ( -B), base of
calculus to the base of defect (B-BD), and alveolar crest
Figure 10. Higher magnification of Figure 5 at level of alveolar crest to the base of defect (A-BD) between submerged and
(AC) demonstrating new cementum (NC)/connective tissue (CT) in- nonsubmerged defects (Table 5).
terface (arrow). Space between cementum, connective tissue and root
surface is artifact. (H&E, original magnification, 40x)

Table 2
Percent of Sections Showing Regeneration of New Cementum Over
Three Types of Tissue
Percent Percent Percent
Treatment over old over
V//-'
over
cementum dentin both r -
-1 " ,'j-Nc
Submerged 19% 19% 62%
Nonsubmerged 50% 50%
:
No new cementum formed.

Table 3 M'
Percent of Sections Showing Regeneration of Periodontal Ligament
in Three Directions
Percent Percent Percent
Figure 11. New attachment apparatus formation coronal to calculus
Treatment parallel perpendicular parallel and reference notch in submerged defect with parallel and perpendicular
to tooth to tooth perpendicular arrangement ofperiodontal ligament fibers (PL). Note new cementum
Submerged 50% 25% 25% (NC), new bone formation (NB), and incorporation ofSharpey'sfibers.
Nonsubmerged* —
(H&E, original magnification, 80x)
*
No new attachment formed
Table 4
Percent of Sections Showing Regeneration for Each Procedure
versus nonsubmerged sites (B-AC 0.99 mm vs 0.11 =
Measurement Submerged Nonsubmerged
mm respectively) but the difference was not significant. 0%
New attachment apparatus 62%
There appeared to be greater loss in height of alveolar New cementum 77% 3%
crest between submerged and nonsubmerged defects New connective tissue 77% 2%
cementum and connective tissue 90% 3%
(A-AC -1.46 mm vs -2.15 mm respectively) but the
= New
New bone 71% 3%
difference was not significant.
Volume 60
Number 12 Histological Evaluation ofNew Periodontal Attachment, I 671

to the calculus reference notch. A combination of ce-

mentum and connective tissue will form more fre-
quently and in greater quantity than cementum or
connective tissue alone. Some new bone formation may
also occur. Complete regeneration of a new attachment
apparatus in a submerged environment, however, ap-
pears to be limited by the amount of new bone and
cementum formation. These findings concur with
Cooke et al.15 who noted that the defects associated
with vitally submerged roots did not completely fill
with bone. Likewise, cementum was observed lining
the root surface at the base of only 2 of the 6 successfully
submerged roots. It also supports the findings of Got-
tlow et al.16 who noted limited bone formation when
membranes were placed to exclude the epithelium.
Epithelial exclusion then is an important factor in the
formation of a new attachment apparatus, but does not
appear to be the only factor necessary to enhance
complete regeneration of periodontal tissues.
Bone Formation
Minimal bone formation will occur from the calculus
reference notch coronally in nonsubmerged defects.
Even if defects are submerged, bone formation will be
limited in amount (x0.96 mm) and frequency of for-
Figure 12. Submerged defect with surface résorption of root coronal mation (71%). Newly formed bone is distinguishable
to calculusreference notch. Note cementoid formation (NC) apical to from existing bony walls by the presence of reversal
résorption bays (R). Also note new bone formation (NB) adjacent to lines, staining characteristics, and close approximation
cementoid formation. (Í1&E, original magnification. 40x)
to pathologically exposed root surfaces (Figs. 8, 11, and
Table 5
12).
Mean Clinical Measurements (mm) at Time of Surgery Cementum Formation
Measurement
Submerged Nonsubmerged
mean mean New cementum may form over old cementum, den-
A-B 3.11 ±1.33+ 3.21 ±0.92 0.44 .53 tin, or both old cementum and dentin in a submerged
B-BD 2.84 ±0.91 2.65 ±1.28 1.06 .34 environment. In 1963, Morris17 suggested that exposed
A-BD 5.95 ± 2.03 5.86 ± 1.80 2.18 .18 dentin may interfere with new cementum formation.
A =
clinical height alveolar crest Wilderman and Wentz18 observed that new cementum
=
base of calculus; BD base of defect
= formation was greatest when dentin was exposed. The
t Mean ± standard deviation (mm) results of our study suggest that cementum may form
equally well over old cementum and dentin. It has also
DISCUSSION been suggested that cementum formation is a response
to notch placement.17 Others have demonstrated that
This study compared the regenerative potential of the presence of a notch per se did not result in increased
the attachment apparatus and component tissues in cementum formation in dogs.19 It appears from the
intrabony defects in submerged and nonsubmerged en- results of our findings that notch placement has no
vironments. The data obtained suggest several impor- direct effect on the location or amount of new cemen-
tant clinical applications. tum formation.
New cementum formation was usually cellular in
Attachment Apparatus Versus Junctional Epithelium nature with incorporation of Sharpey's fiber attachment
Epithelial exclusion is an important factor in the type (Figs. 9 and 11 ). Stahl suggested that acellular cemen-
of attachment that forms after flap curettage and root tum is more conducive to fiber attachment than cellular
planing procedures. In nonsubmerged defects, the for- cementum.20 The incorporation of Sharpey's fibers was
mation of a long junctional epithelium is to be expected a consistent finding of cellular cementum in our study.
after flap curettage and root planing. If the epithelium Hence, the type of cementum formation may not be a
is excluded, new cementum, new connective tissue, or limiting factor in fiber attachment as previously sug-
new cementum and connective tissue will form coronal gested.
 J. Periodontol.
672 Bowers, Chadroff, Carnevale, Mellonig, Corlo, Emerson, Stevens, Bömberg December 1989

Periodontal Ligament Formation gingival connective tissue was placed directly over cut
In a submerged environment, the periodontal liga- dentin surfaces and various combinations of bony walls
surrounded the submerged roots. It was encouraging
ment may be oriented parallel, perpendicular, or par-
that in no instance was extensive root résorption of
allel and perpendicular in the same defect. Sharpey's
fibers will be incorporated in bone and cementum in dentin or cementum noted in this study. Surface ré-
both parallel and perpendicular arrangements (Fig. 11). sorption of both dentin and cementum, however, was
Melcher stated that functional (perpendicular) orienta- a common finding. Resorption bays were frequently
tion is unlikely without occlusal forces acting on the lined with cementoid or newly formed cellular cemen-
tum. Surface résorption was such a consistent finding
teeth.21 Occlusion was not a factor in submerged vital
that it appeared to the examiners that it may be a
roots. It is possible, however, that the forces created by
dentures may have been sufficient to stimulate the prerequisite for new cementum formation on previ-
functional orientation in some submerged defects. ously exposed root surfaces.
It has been suggested from animal studies that the
Pulpal Reaction to Coronectomy and Root Submersion cells responsible for root résorption originate from sur-
Pulp death does not appear to be a common sequela gical flaps.23 In humans, the placement of gingival
of coronectomy. It also does not appear to be a sequela connective tissues in contact with dentin or cementum
of debridement and root planing of deep intrabony does not appear to result in extensive root résorption.
In fact, new cementum formation was occasionally
defects, which in some instances extended to the apex. observed over the severed dentin surface (Figs. 14, 15,
Pulp tissue was not always present for histological eval- and 16). Likewise, cells originating in bone do not
uation, but when it was observed, there was often
evidence of secondary dentin formation opposite the appear to produce extensive root résorption. It is pos-
sible then, that cells derived from other sources, such
calculus reference notch in both submerged and non-
as bone, can express the phenotype for new cementum
submerged roots (Fig. 13). It is not clear how pulpal formation. This hypothesis has been proposed by other
tissue survives and functions after coronectomy. All
sections with pulpal tissue are presently being evaluated
authors25-26 and warrants further evaluation in humans.
by a pulp pathologist who may be able to provide some
explanation.
Root Resorption and Ankylosis
Based on the results of this study, extensive root
résorption and ankylosis are not common sequelae of
vital root submersion in humans. Several authors have
demonstrated extensive root résorption of reimplanted
teeth in animals.22"24 It has been demonstrated in ani-
mals that, if cells from the gingival connective tissue22
or the alveolar bone23 contact the root surfaces, exten-
sive root résorption will occur. Based on these studies,
we anticipated that root résorption and ankylosis would
be a common occurrence on submerged roots since

Figure 14. New bone formation did not occur coronal to calculus
reference notch (B) in this submerged defect. New cementum has
formedfrom calculus notch to cut surface ofdentin (D) and over dentin
(arrows). (H&E, original magnification, 40x) Higher magnification
Figure 13. Dental pulp (P) of submerged defect demonstrating sec- of cementum forming over severed dentin surface is shown in Figures
ondary dentin formation (SD). (H&E, original magnification, 40x) 12 and 13.
Volume 60
Number 12 Histological Evaluation ofNew Periodontal Attachment, I 673
F. Extensive root résorption, ankylosis, and pulp
death are not common sequelae of this treatmejit.
2. In nonsubmerged intrabony defects treated by flap
curettage and root planing:
A. The formation of a new attachment apparatus
was not observed on previously exposed root sur-
faces.
B. A small amount of new cementum, connective
tissue, or bone formed on previously exposed root
surfaces.
C. Root résorption, ankylosis, and pulp death are
not common sequelae of this treatment.
ACKNOWLEDGMENTS
This study was supported by Grant DE 06250 from the National
Figure 15. Higher magnification of severed dentin surface in Figure Institute of Dental Research; Research Grant M0095.001.003, US
11. Note cellular cementum (NC) overlying dentin (D). (H&E, original
magnification, 40x) Navy Tissue Bank, NMRI, NNMC; and research funds from the
Armed Forces Institute of Pathology.

REFERENCES
.V '·; .
JG, Kowalski CJ. Primate model for testing periodontal
1. Catón
treatment features: II. Production of contralaterally similar lesions. /
Periodontal 1976;47:506.
2. Caton JG, Zander HA. Osseous repair of an inffabony pocket
without new attachment of connective tissue. J Clin Periodontol
1976;3:54, 1976.
3. Caton J, Nyman S. Histometric evaluation of periodontal sur-
gery. I. The modified Widman flap procedure. J Clin Periodontol
1980;7:212.
4. Caton J, Nyman S, Zander H. Histometric evaluation of peri-
D odontal surgery. II. Connective tissue attachment levels after four
regenerative procedures. J Clin Periodontol 1980;7:224.
5. Mellonig JT. Histologie evaluation of freeze-dried bone allo-
grafts in periodontal osseous defects. J Dent Res 1981. 60 (Spec Issue):
(Abstr 388).
6. Bowers GM, Schallhorn RH, Mellonig JT. Histologie evalua-
Figure 16. Higher magnification of severed dentin surface in Figure tion of new attachment in human intrabony defects, a literature
11 demonstrating cellular cementum formation (NC) and connective review. J Periodontol 1982;53:509.
tissue attachment (CT) with no indication of extensive root résorption. 7. Cole RT, Crigger M, Bogle G, et al. Connective tissue regener-
(H&E, original magnification, 40x) ation to periodontally diseased teeth. / Periodont Res 1980; 15:1.
8. Moskow BS, Karsh F, Stein SD. Histological assessment of
autogenous bone graft—a case report and critical evaluation. J Per-
CONCLUSIONS iodontol 1979;50:291.
9. Gara GG, Adams DF. Implant therapy in human intrabony
1. In submerged intrabony defects treated by flap pockets: A review of the literature. J West Soc Periodont Periodont
curettage and root planing: Abstr 1981;29:2.
A. It is possible to form a new attachment appa- 10. Egelberg J. Regeneration and repair of periodontal tissues. /
ratus on previously exposed root surfaces. Periodont Res 1987;22:233.
11. Bowers GM, Granet M, Stevens M, et al. Histologie evaluation
B. Significantly more new attachment apparatus
of new attachment in humans. A preliminary report. J Periodontol
and component tissues will form in submerged
1985;56:381.
defects than in nonsubmerged sites. The amount 12. FroumSJ, Thaler R, Scopp IW, Stahl SS: Osseous autografts.
of new attachment apparatus formed is limited by II. Histologie response to osseous coagulum-bone blend grafts. J
the amount of new bone and cementum formation. Periodontol 1975;46:656.
C. A new attachment apparatus and component 13. Dragoo MR, Sullivan HC. A clinical and histological evalua-
tion of autogenous iliac bone grafts in humans. Part II. External root
tissues formed more frequently, in submerged de-
résorption. J Periodontol 1973;44:614.
fects than in nonsubmerged sites. 14. Dragoo MR. Regeneration of the Periodontal Attachment in
D. New cementum formed over old cementum Humans. Philadelphia: Lea & Febiger, 1981.
and dentin, but more often formed over old ce- 15. Cooke RT, Hutchins LH Jr., Burkes EJ Jr. Periodontal osseous
mentum and dentin in the same defect. defects associated with vitally submerged roots. J Periodontol
E. The periodontal ligament was oriented parallel, 1977;48:249.
16. Gottlow J, Nyman S, Karring T, Lindhe J. New attachment
perpendicular, or parallel and perpendicular in the formation as the result of controlled tissue regeneration. J Clin
same defect. Periodontol 1984;! 1:494-503.
 J. Periodontol.
674 Bowers, Chadroff, Carnevale, Mellonig, Corlo, Emerson, Stevens, Bömberg December 1989

17. Morris ML. Healing of human periodontal tissues following 1980;7:96.

surgical detachment: The effect of two horizontal nicks in the depo- 24. Karring T, Nyman S, Lindhe J, Surial M. Potentials for root
sition of new cementum. Periodontics 1963; 1:147. résorption during periodontal wound healing. J Clin Periodontol
18. Wilderman MM, Wentz FM. Repair of a dentogingival defect 1984;11:41.
with a pedicle flap. J Periodontol 1965;36:218. 25. Melcher AH, Cheong T, Cox J, Nemeth E, Shiga A. Synthesis
19. Garrett S, Bogle G, Adams D, Egelberg J. The effect of of cementum-like tissue in vitro by cells cultured from bone: A light
notching into dentin on new cementum formation during periodontal and electron microscope study. J Periodont Res 1986;21:592.
wound healing. J Periodimi Res 1981:16:358. 26. Aukhil I, Simpson DM, Johnson MC, Koch G. Progenitor
20. Stahl SS. Repair potential of the soft tissue-root interface. J cell kinetics during guided tissue regeneration in experimental peri-
Periodontol 1977;48:545. odontal wounds. J Periodont Res 1988;23:107-117.
21. Melcher AH. Repair of wounds in the periodontium of the
rat. Influence of periodontal ligament in Osteogenesis. Arch Oral Bio!
1970;15:1191.
22. Nyman S, Karring T, Lindhe J, Planteu S. Healing following
implantation of Periodontitis affected roots into gingival connective Send reprint requests to: Dr. Gerald M. Bowers, Department of
tissue. / Clin Periodontol 1980;7:394-401. Periodontics, University of Maryland, Baltimore College of Dental
23. Karring T, Nyman S, Lindhe J. Healing following implanta- Surgery, 666 West Baltimore Street, Baltimore, Maryland 21201.
tion of Periodontitis affected roots into bone tissue. J Clin Periodontol Accepted for publication June 14, 1989.