UV Visible Spectroscopy

Q 1
Question Qentify the CORRECT statements of the following----
A UV Spectroscopy involves vibrational transitions
B IR Spectroscopy involves electronic transitions
C NMR spectroscopy involves change in spin state of atomic nuclei
D Mass spectroscopy involves rotational transition
Q 2
Question Which of the following electronic transition requires least energy?
A σ → σ*
B n → π*
C n→ σ *
D π →π*
Q 3
Question Shift of UV absorption bands towards lower wavelength is called as-----
A Hyperchromic shift
B Bathochromic shift
C Hypochromic shift
D Hypsochromic shift
Q 4
Question Identify the CORRECT statement of followings?
A Radiation with shorter wavelengths have high energies
B Energy of EMR is directly proportional to its frequency
C Radiation with longer wavelengths have higher energies
D Energy of EMR is directly proportional to its wavenumber
Q 5
Question Spectroscopy deals with interaction of electromagnetic radiation with matter. What is the
speed of this radiation in vacuum in m/s?
A 3x 1010
B 3x 10-10
C 3x 108
D 3x 10-8
Q 6
Question Lambert’s law states that the intensity of light decreases with respect to ----
A Concentration
B Thickness of medium
C Composition
D Volume
Q 7
Question Which of the following detectors does not require a battery and is also known as barrier
layer cell?
A Photomultiplier tube
B Photovoltaic cell
C Photo emissive tubes
D Photo reflector
Q 8
Question What is the unit of molar absorptivity (€) in formula A = € b c?
A L mol-1 cm-1
B L gm-1 cm-1
C cm
D No unit
Q 9
Question π→π* electronic transitions is observed in which of the following compounds?
A Alkane
B Saturated compounds
C Alkyl halQes
D Conjugated polyenes.
Q 10
Question Which of the following statements is false about double beam spectrophotometer?
A Deuterium lamp is used as light source
B Beam splitter is used to get single beam of light
C Glass tube is used as sample holder
D Photovoltaic cell is used as detector
Q 11
Question Absorption of radiation in the UV range attributable to n→π* electronic transitions is
characteristic of which of the following types of compounds?
A Aromatic hydrocarbons.
B Unsaturated carbonyl compounds.
C Non-conjugated polyenes.
D Conjugated polyenes.
Q 12
Question The Excitation filter is placed in between
A Source and cell
B Cell and detector
C Source and detector
D Anywhere
Q 13
Question Which of the following is the purpose of the beam splitter in double beam colorimeter?
A Splits beam into two equal intensity beams
B Splits beam in such a way that sample beam has higher intensity
C Splits beam in such a way that reference beam has higher intensity
D Merge two equal intensity beams into single beam
Q 14
Question In which of the following ways, absorption is related to transmittance?
A Absorption is the logarithm of transmittance
B Absorption is the reciprocal of transmittance
C Absorption is the negative logarithm of transmittance
D Absorption is a multiple of transmittance
Q 15
Question What is the wavelength range of FAR UV region of light?
A 400 nm – 700 nm
B 10 nm – 200 nm
C 200 nm to 400 nm
D 10 nm to 400 nm
Q 16
Question In which compounds "π" electrons are present
A Saturated compounds
B Unsaturated compounds
C non bonded
D not present in any compound
Q 17
Question Which of the following electronic transition requires highest energy?
A σ → σ*
B n → π*
C n→ σ *
D π →π*
Q 18
Question Shift of UV absorption bands towards longer wavelength is called as-----
A Hyperchromic shift
B Bathochromic shift
C Hypochromic shift
D Hypsochromic shift
Q 19
Question n→π* electronic transitions is observed in which of the following compounds?
A Aromatic hydrocarbons.
B Unsaturated carbonyl compounds.
C Non-conjugated polyenes.
D Conjugated polyenes.
Q 20
Question One of the following detector of UV spectroscopy has more sensitivity and amplification
power
A Photocell
B Barrier layer cell
C Photo Multiplier Tube
D Photo tube
Q 21
Question Which of the following wavelength ranges is associated with UV spectroscopy?
A 0.8 – 500 µm
B 400-1000nm
C 380 – 750 nm
D 10 nm – 400 nm
Q 22
Question In UV spectroscopy occurring of absorbance n – π * transition due to
A Bonded electrons
B Non bonded electrons apart from C and H
C Non bonded electrons of C and H
D Bonded electrons of C and H
Q 23
Question UV Spectroscopy is working on which principal.
A partition
B absorption
C adsorption
D emission
Q 24
Question An auxochromes is one which is
A Colour enhancing
B A group or atom with lone pair of electrons
C extending conjugation
D All of these
Q 25
Question Ultra violet spectroscopy is useful for the detection of-------
A Functional group
B Extent of conjugation
C Geometrical isomer
D All of these
Q 26
Question Which filter are absorbed UV radiation and transmit visible radiation ?
A Primary filter
B Secondary filter
C A and B
D None of this
Q 27
Question Beer Lambert’s law gives the relation between which of the following?
A Reflected radiation and concentration
B Scattered radiation and concentration
C Energy absorption and concentration
D Energy absorption and reflected radiation
Q 28
Question What is the unit of molar absorptivity or absorptivity which is used to determine
absorbance A in Beer Lambert’s formula?
A L mol-1 cm-1
B L gm-1 cm-1
C Cm
D No unit
Q 29
Question Collimator is used for?
A To reform the image of the entrance slit.
B To get narrow source
C To fall on sample cell
D To disperse radiation
Q 30
Question Which device is used to isolate the radiation of the desired wavelength from wavelength
of the continuous spectra?
A Monochromator
B Radiation source
C Recorder
D None of this
 Fluorimetry

Q 1
Question Which of the following substitution increase the fluorescence of aromatic compounds?
A Ortho substitution
B Meta substitution
C Para substitution
D All of the above
Q 2
Question Phosphorescence is the phenomenon of
A Emission of electrons by interacting with radiation
B Emission of radiation by interacting with electrons
C Reemission of previously absorbed radiation
D Absorption of emitted radiation
Q 3
Question Electron spin is retained in which of the following phenomenon?
A Fluorometry
B Phosphorimetry
C Mass spectroscopy
D IR spectroscopy
Q 4
Question The fluorescence intensity increases with increase in
A Viscosity
B Temperature
C Photodecomposition
D Concentration of oxygen
Q 5
Question Which of the following factor decreases fluorescence intensity?
A Conjugation
B Electron donating groups
C Presence of oxygen
D RigQity
Q 6
Question Inner filter effect is the fluorescence quenching due to
A Absorption of excitation radiation
B Absorption of luminescent radiation
C Both a and b
D Collisional deactivation
Q 7
Question Luminescence is the phenomenon of
A Emission of electrons by interacting with radiation
B Emission of radiation by interacting with electrons
C Reemission of previously absorbed radiation
D Absorption of emitted radiation
Q 8
Question How many mm path length sample cell in Fluorimeter?
A 10
B 20
C 30
D 40

IR Spectroscopy

Q 1
Question Which of the following molecules will show infrared spectrum?
A CO2
B F2
C I2
D CL2
Q 2
Question Which of group the followings shows absorption band in wave number range 1710 cm -1
to 1750 cm-1?
A OH
B C=O
C CH
D NH
Q 3
Question Which of the following is NOT the function of drive mechanism in Fourier Transform
Infrared Spectrophotometer?
A Movement of mirror to obtain a satisfactory interferogram
B Acquire a good interferogram pattern
C Allow 50% of the beam to pass
D Keep the speed of the moving mirror constant
Q 4
Question Which of the following option is CORRECT in terms of wavelength for the different
types of IR spectrometer?
A Near IR: 0.8 – 2.5 µm
B MQ IR: 0.8 – 2.5 µm
C Far IR: 2.5 – 50 µm
D MQ IR: 50 – 100 µm
Q 5
Question Which of the following is not a composition of Nernst glower or Nernst filament?
A OxQes of Zirconium
B Oxides of Sodium
C Oxides of Yttrium
D Oxides of Thorium
Q 6
Question Which of the following is the principle of Golay cell which is used as a detector in IR
spectroscopy?
A Expansion of gas upon heating
B Increase in resistance due to increase in temperature and vice versa
C Temperature difference gives rise to a potential difference in the material
D Decrease in resistance due to increase in temperature
Q 7
Question Which of the following technique is used for preparing solid samples in IR spectroscopy?
A Spraying
B Pellet techniques
C Spraying
D Ionization
Q 8
Question In a solid sample treatment technique, the finely ground solid sample is mixed with mineral oil to
make a thick paste which is then spread between IR transmitting windows. What is the
name of this solid sample treatment technique?
A Pressed pellet
B Solids run in solution
C Solid films
D Mull technique
Q 9
Question What is the relation between frequency of IR absorption and the reduced mass?
A Frequency is directly proportional to reduced mass
B Frequency is inversely proportional to square root of reduced mass
C Frequency is independent of the reduced mass
D Frequency is directly proportional to square of reduced mass
Q 10
Question Bolometer, a type of detector, is also known as:
A Resistance temperature detector (RTD)
B Thermistor
C Thermocouple
D Golay cell
Q 11
Question Which of the following is used as a source in IR Spectroscopy?
A Tungsten halogen lamp
B Hollow cathode lamp
C Xenon arc
D Incandescence Lamp
Q 12
Question Which of the following can be introduced into the ionization chamber directly?
A SolQ samples with low vapor pressure
B SolQ samples with high vapor pressure
C LiquQ samples with low density
D LiquQ samples with high density
Q 13
Question Which of the following probes are used for the introduction of sample in the ionization
chamber?
A Silica
B Quartz
C Graphite
D Silver
Q 14
Question Which of the following is the principle of Golay cell which is used as a detector
in IR spectroscopy?
A Expansion of gas upon heating
B Increase in resistance due to increase in temperature and vice versa
C Temperature difference gives rise to a potential difference in the material
D Decrease in resistance due to increase in temperature
Q 15
Question In a solQ sample treatment technique, the finely ground solQ sample is mixed with min-
eral oil to make a thick paste which is then spread between IR transmitting win-
dows. What is the name of this solQ sample treatment technique?
A Pressed pellet
B Mull technique
C SolQ films
D SolQs run in solution
Q 16
Question The most commonly used mulling reagent in IR spectroscopy is which of the following
A CHCI3
B Nujol
C Hexachlorobutadiene
D Chlorofluoro carbon oil
Q 17
Question Select the number of vibrational degrees of freedom of benzene from the following
A 15
B 25
C 30
D 35
Q 18
Question What kind of vibrational changes occur at lower frequency in IR spectroscopy?
A Stretching vibration
B Bending or stretching depending on the media
C Bending Vibrations
D None of above
Q 19
Question IR radiations can be expressed in which of the following forms
A Transmittance
B Nanometer
C Wavelength
D Absorbance
Q 20
Question Which of the followings functional group shows absorbance 1700 cm-1?
A Ketone
B Amines
C Halogens
D SuphQes

Flame Photometry
Q 1
Question In Flame emission photometers, the measurement of _____________ is used for
qualitative analysis.
A Color
B Intensity
C Velocity
D Frequency
Q 2
Question Atomic emission spectroscopy is
A The measurement of intensity of emitted light at a particular wave length from the atoms
that are exited thermally
B The measurement of absorbance of emitted light at a particular wave length from the
atoms that are exited thermally.
C The measurement of intensity of emitted light at a particular wave length from the atoms
that are excited by monochromatic light.
D The measurement of intensity of absorbed light at a particular wave length from the
atoms that are exited thermally.
Q 3
Question Flame ionization detector used in-----
A TLC
B HPLC
C Ion exchange chromatography
D Gas chromatography
Q 4
Question Laminar flow burner used in Flame photometers is also known as ____________
A Turbulent burner
B Premix burner
C Total consumption burner
D Nozzle mix burner
Q 5
Question The range of temperatures attainable by acetylene-air mixture is---OC
A 2550-2700
B 2000-2100
C 3050-3150
D 2100-2400
Q 6
Question Choose correct sequence of flame photometry?
A Sample residue → excited state atoms → Return in ground state → Emission of
radiation
B Sample residue → ground state → excited state → Emission of radiation
C Emission of radiation → excited state →ground state → Sample residue
D Sample residue → ground state → excited state → Emission of radiation
Q 7
Question Which of the following is application of flame photometry?
A To determine functional group
B To study of chemical structure
C To assay of drug
D To estimate metallic ions like sodium, potassium, etc
Q 8
Question Laminar flow burner used in Flame photometers is also known as ______.
A Turbulent burner
B Premix burner
C Total consumption burner
D None of the above
Q 9
Question In flame photometry, the wavelength of _____ is characteristic.
A Absorbed light
B Transmitted light
C Scattered light
D Emitted light
Q 10
Question Following element produces yellow coloured flame in flame photometry?
A Copper
B Sodium
C Lithium
D Barium
Q 11
Question The various processes in the flame photometry are as ------------------.
A Desolvation
B Vapourisation
C Atomization
D All of the above
Q 12
Question A mixture of the following gases can be used in flame photometry to get a temperature of
20450C
A Hydrogen & nitrous oxide
B Acetylene & oxygen
C Hydrogen & air
D Hydrogen & oxygen
Q 13
Question In Atomic Emission Spectroscopy, _____________is widely used for the electrodes.
A Tungsten
B Graphite
C Sodium
D Nickel
Q 14
Question The emission spectrum produced by hollow cathode lamp is a sharp line spectrum
consisting of----------.
A Cathode Material
B Filled gas
C Both A&B
D Anode material
Q 15
Question Which of the following is not a detector used in Flame emission photometers?
A Photo tube
B Photovoltaic cell
C Chromatogram
D Photoemisive Tube

Atomic Absorption Spectroscopy

Q 1
Question Which of the following could be used as dielectric spacer in interference filters used in
Absorption Spectroscopy?
A Graphite
B MgF2
C Fe
D AgNO3
Q 2
Question Which of the following is NOT TRUE about Absorption spectroscopy?
A It involves transmission
B Scattering is kept minimum
C Reflection is kept maximum
D Intensity of radiation leaving the substance is an indication of concentration
Q 3
Question Which of the following is the function of the Flame or Emission system in Atomic Ab-
sorption Spectroscopy?
A To filter unwanted components
B To polychromatic light into monochromatic light
C To reduce the sample into atomic state
D To break the steady light into a pulsating light
Q 4
Question Which of the following is not a step in atomic absorption spectroscopy (AAS)?
A Particles are adsorbed onto a stationary phase.
B Atoms absorb light.
C A solution is vaporised.
D A calibration curve is constructed.
Q 5
Question Which of the following is used as a source in Atomic Absorption Spectroscopy?
A Tungsten halogen lamp
B Hollow cathode lamp
C Xenon arc
D Globar

Nepheloturbidimetry
Q 1
Question In Turbidimetry, The intensity of the transmitted light is usually measure at angle--------
A 90°
B 45°
C 135°
D 180°
Q 2
Question The principle involved in turbidimetry is the measurement of
A Absorbed light
B Scattered light
C Emitted light
D Transmitted light
Q 3
Question The intensity of the transmitted light is usually measure at which angle?
A 90°
B 0°
C 180°
D 270°
Q 4
Question Nephelometry is much similar to following analytical technique.
A Flame photometry
B Colorimetry
C Fluorimetry
D Mass spectroscopy
Q 5
Question Which of the following is the light source in Nepheloturbidometer?
A Tungsten filament lamp
B Mercury lamps
C Both A & B
D None of above

Introduction To Chromatography
Q 1
Question In Liquid chromatography, the stationary phase is made of _________ and the mobile
phase is made of _________
A Solid, liquid
B Liquid, Solid
C Liquid, gas
D Solid, gas
Q 2
Question In chromatogram, area of peaks represents ---
A Components of the sample
B Amount of component in the sample
C Number of components in mixture
D None of the above
Q 3
Question How many percentages of the Beta cellulose in stationary phase?
A 2-4%
B 0.3-1%
C 0.2 – 3 %
D 0.03-0.1 %
Q 4
Question The components with less affinity towards the mobile phases --
A Travels slow
B Travels fast
C Can’t predicted
D None of the above
Q 5
Question Which type of packing technique of column not commonly used
A Wet packing
B Dry packing
C Semiwet Packing
D Semidry packing
Q 6
Question Which is not requirement of mobile phase?
A It should be not toxic.
B It should be easily available.
C It should be chemically inert.
D It should be chemically active.
Q 7
Question Which of the following is NOT a type of Partition chromatography?
A Paper Chromatography
B TLC
C HPLC
D GLC

Adsorption & Column Chromatography

Q 1
Question Which of the following is an example of partition chromatography
A Adsorption column chromatography
B High Performance Thin Layer Chromatography
C Paper chromatography
D Gas solid chromatography
Q 2
Question In column chromatography, the length of column depends upon
A Number of compounds to be separated
B Mobile phase
C Stationary phase
D Development technique
Q 3
Question Which of the commonly used adsorbent in Column Chromatography
A Sucrose
B Starch
C Talc
D Silica Gel
Q 4
Question Which of the following will improve the efficiency of the separation process in liquQ
chromatography?
A Increase in sample size, increase in column diameter
B Reduction in sample size, increase in column diameter
C Increase in sample size, reduction in column diameter
D Reduction in sample size, reduction in column diameter
Q 5
Question In adsorption chromatography-------
A Stationary phase is solid, while mobile phase is liquid
B Both the phases are liquid
C Both the phases are solid
D Stationary phase is liquid, while mobile phase is solid
Q 6
Question Which of the following adsorbents used for column adsorption chromatography has
maximum adsorptive power?
A Silica gel
B Magnesium oxide
C Aluminum oxide
D Calcium carbonate
 Thin Layer Chromatography

Q 1
Question Which is the following best technique for preparation of TLC plates
A Pouring
B Dipping
C Spraying
D Spreading
Q 2
Question In TLC, Rx value is
A The ratio of distance travelled by the standard and distance traveled by the sample.
B The ratio of distance travelled by the sample and distance traveled by the standard.
C The ratio of distance travelled by the solute to the distance travelled by the solvent.
D The ratio of distance travelled by the solvent to the distance travelled by the solute.
Q 3
Question Which ONE of the following is used as adsorbents for TLC
A Silica gel
B Alumina
C Kiesleguhr
D All of the above
Q 4
Question The Quantification of spot on TLC plate done by all of following except
A Spraying with reagent
B Under Microscope
C Fluorescence
D Fluorescence adsorbent
Q 5
Question Glass plates in thin layer chromatography can be coated by which of the following
techniques?
A Spreading
B Pouring
C Spraying
D All of the above
Q 6
Question The ideal thickness of silica gel G layer on in TLC should be-
A 0.15 micrometer
B 0.25 micrometer
C 0.5 micrometer
D 1 micrometer

Paper Chromatography
Q 1
Question In which of the following type of paper chromatography the mobile phase move vertically
in upward direction over sheet of paper?
A Ascending paper chromatography
B Descending paper chromatography
C Radial paper chromatography
D Ascending – descending chromatography
Q 2
Question Which type of filter paper is mostly used in paper chromatography?
A Butter paper
B Sample paper
C Whatmann filter paper
D Filter paper
Q 3
Question In which of the following type of paper chromatography the mobile phase move horizon-
tally over a circular sheet of paper?
A Ascending paper chromatography
B Descending paper chromatography
C Radial paper chromatography
D Ascending – descending chromatography
Q 4
Question In paper Chromatography, circular development technique is also called as
A Ascending development
B Decending development
C Radial development
D Two Dimensional development
Q 5
Question Paper chromatography is more suited to which of the following principles?
A Electrical mobility of ionic species
B Partition chromatography
C Both A and B
D Adsorption chromatography
Q 6
Question Following which is not types of paper chromatography---------.
A Radial chromatography
B Ascending chromatography
C Thin layer chromatography
D Two dimensional chromatography

Electrophoresis
Q 1
Question In paper electrophoresis, which type of paper is used?
A Filter paper
B Executive bond paper
C Whatman filer paper
D Hydrophilic paper
Q 2
Question An improved version of gel electrophoresis is ---------
A Capillary gel electrophoresis
B Paper electrophoresis
C Cellulose acetate electrophoresis
D SDS electrophoresis
Q 3
Question In electrophoresis, separation depends on….
A Affinity
B Partition
C Movement of ions under influence of electric fields
D All of the above
Q 4
Question The technique electrophoresis, for the separation of charged molecules was developed
 by
A Tswett
B Tiselius
C Tsvedberg
D Sanger

Gas Chromatography
Q 1
Question Which of the following conditions will cause the efficiency of the column to increase?
A Plate number becomes greater, plate height becomes smaller
B Plate number becomes smaller, plate height becomes smaller
C Plate number becomes greater, plate height becomes larger
D Plate number becomes smaller, plate height becomes larger
Q 2
Question Which of the following is not a feature of carrier gas used in gas chromatography?
A It must be chemically inert
B It should be suitable for the detector employed
C It should not be completely pure
D It should be cheap
Q 3
Question Which two important criteria for compounds two be analysed by Gas Chromatography
A Volatility & Thermoliability
B Volatility & Thermostability
C Non-Volatility & Thermoliability
D Non-Volatility & Thermostability
Q 4
Question In Gas Chromatography, SCOT column means
A Secondary Coated Open Tubular Column
B Support Coated Open Table Column
C Support Coated Open Tubular Column
D Support Coated On Table Column
Q 5
Question Which of the following is not a feature of carrier gas used in gas chromatography?
A It must be chemically inert
B It should be suitable for the detector employed
C It should not be completely pure
D It should be cheap
Q 6
Question In which of the following methods liquid samples are injected into the column in gas
chromatography?
A Gas tight syringe
B Micro-syringe
C Rotary sample valve
D Solid injection syringes
Q 7
Question If the mobile phase is gas in partition chromatography, it is called as----
A Solid Chromatography
B Gas liquid chromatography
C Liquid gas chromatography
D Liquid liquid chromatography
Q 8
Question Which of the following is not a feature of carrier gas used in gas chromatography?
A It must be chemically inert
B It should be suitable for the detector employed
C It should not be completely pure
D It should be cheap
Q 9
Question Which of the following is the commonly used support material for the packed column in
gas chromatography?
A Glass
B Metal
C Diatomaceous earth
D Stainless steel
Q 10
Question Which of the following detector consisting of radioactive isotopes in Gas
Chromatography?
A Argon ionization detector
B Thermal conductivity detector
C Electron capture detector
D Flame ionization detector
Q 11
Question Which of the following detectors used in gas chromatography is also called as
Katharometer?
A Thermionic Detectors
B Thermal conductivity Detectors
C Both A and B
D Electron capture Detectors
Q 12
Question The parameter in the elution curve that is proportional to the concentration of a compound
in gas chromatography effluent is the
A Number of peaks
B Width of the peaks
C Area under the peaks
D Shape of the peaks

High Performance Liquid Chromatography (HPLC)

Q 1
Question Which of the following HPLC detector works according to emission of light?
A Electrochemical detectors
B Fluorescence detectors
C Refractive index detectors
D Thermal conductivity detectors
Q 2
Question In HPLC, P stands for -----
A Performance
B Pressure
C Both A & B
D None
Q 3
Question Which of the following phase mode HPLC used for analysis of drugs & pharmaceuticals?
A Partition Phase
B Adsorption Phase
C Normal Phase
D Reverse Phase
Q 4
Question In HPLC, a detector that measures property possessed by both mobile phase & solute is
called ---------------------
A Bulk property detector
B Solute property detector
C Minute property detector
D Major property detector
Q 5
Question Which of the following HPLC detector works according to Snell’s law?
A Electrochemical detectors
B Fluorescence detectors
C Refractive index detectors
D Thermal conductivity detectors
Q 6
Question The pressure used in HPLC is
A 1000-3000 psi
B 1000-5000 psi
C 1000-6000 psi
D 2000-6000 psi
Q 7
Question A gradient elution in HPLC is one in which the composition of the solvent.
A remains constant
B is changed continously or in series of steps
C Both A & B
D None of both
Q 8
Question Identify the solute property detector used in HPLC
A FTIR Detector
B Refractive Index Detector
C Fluorescence Detectors
D None of the above
Q 9
Question What is the full form HPLC?
A High performance liquQ chromatography
B High pressure liquid chromatography
C High polarity liquid chromatography
D All of the above

Ion Exchange Chromatography

Q 1
Question Which material is used as supporting system in ion exchange chromatography?
A Phenol
B Metal
C Resin
D Carbohydrates
Q 2
Question Which of the following example of commercially available cation exchange resin used in
ion exchange chromatography?
A Shellac
B Rosin
C Zeolite
D Copal
Q 3
Question Which method is used for preparing of demineralized water?
A Gas Chromatography
B Ion exchange Chromatography
C Paper chromatography
D HPTLC
Q 4
Question In Ion Exchange Chromatography, Which of the following factor affect ion exchange
separation?
A Nature of ion exchange resins
B Nature and properties of ion exchange resins
C Properties of ion exchange resin
D Cation exchange resin
Q 5
Question Ion exchange chromatography is used for separation of
A Amino acids
B Aldehydes
C Fatty acids
D Hydrocarbons
Q 6
Question Anion exchange resin is regenerated by the
A Charging column with strong acid
B Charging column with weak acid
C Charging column with strong base
D Charging column with weak base
Q 7
Question In ion exchange capacity of a resin is dependent on
A The total molecular weight of the resin
B The total number of ion active groups
C Length of the ion exchange resin
D Solubility of the ion exchange resin

Gel Chromatography
Q 1
Question Which of the following chromatography is also called as Filtration chromatography?
A Ion exchange chromatography
B TLC
C HPLC
D Gel chromatography
Q 2
Question In which of the chromatography, the components of mixture are separated as per their
molecular size?
A Paper chromatography
B TLC
C HPTLC
D Gel chromatography
Q 3
Question As per Gel Chromatography, in analytical applications, sample of --------- percent of total
bed volume is used
A 01-Mar
B 02-May
C 03-Jul
D 04-Sep
Q 4
Question Which of the following chromatography is also called as Size exclusion chromatography?
A Ion exchange chromatography
B TLC
C HPLC
D Gel chromatography
Q 5
Question In gel permeation chromatography, which type of particles are eluted first
A Larger molecules
B Moderate size molecules
C Smaller molecules
D None of the above

Affinity Chromatography
Q 1
Question In affinity chromatography, the matrix is
A an inert support to which a ligand can be directly or indirectly coupled.
B an chemically active support to which a ligand can be directly or indirectly coupled.
C an mechanical support to which a ligand can be directly or indirectly coupled.
D an rigid gel support to which a ligand can be directly or indirectly coupled.
Q 2
Question In affinity chromatography, the most useful matrix materials are ---------------------------
A Agarose & Polyacrylamide
B Dextrans & Sephadex
C Starch & Rubber
D Rubber & Polystyrenes
Q 3
Question In affinity chromatography, spacer arm is used to --------------------
A Improve binding between ligand and target molecule by overcoming any effects of
steric hindrance.
B Improve binding between ligand and matrix by overcoming any effects of steric hin-
drance.
C Improve binding between ligand and antigen by overcoming any effects of steric hin-
drance.
D Improve binding between ligand and antigen-antibody by overcoming any effects of steric
hindrance.
Q 4
Question Affinity chromatography is based on
A Adsorption phenomenon
B Partition phenomenon
C Both a and b
D None of the above