Soil Fertility AGR521: Available Micronutrients

SOIL FERTILITY
AGR521
LABORATORY 5
TITLE: AVAILABLE MICRONUTRIENTS
STUDENTS NAME & 1. MOHD HAFIZI BIN IBRAHIM
MATRIX NO 2021878412
2. MUHAMAD SYAMSUNIZAM BIN MOHD ZAINI
2021231328
GROUP AT2203A
PART PART 2
LECTURER NAME ANISAH BINTI MOHAMMED
ACADEMIC SESSION MARCH – AUGUST 2022

LABORATORY REPORT 5
A. TITLE
AVAILABLE MICRONUTRIENTS
B. INTRODUCTION
The micronutrients B, Cl, Cu, Fe, Mn, Mo and Zn are the seven elements essential for plants at
requirement levels of less than 0.10% in the plant’s dry matter. Micronutrients in soil are:
1. Elements in primary and secondary minerals
2. Adsorbed to mineral and organic matter surfaces
3. Incorporated in organic matter and microorganisms
4. Present in solution
Extraction reagents, their interacting factors and the range in critical level values have been
summarized by Sims and Johnson (1991) as shown in the following table.
C. OBJECTIVE
1. To determine available micronutrients
D. APPARATUS MATERIAL AND PROCEDURE
1. Falcon tube
2. Filter paper
3. 50 mL plastic vial
4. Mehlich No. I reagent (0.05N HCl in 0.025N H2SO4)
E. REAGENT PREPARATION
1. Mehlich No. 1 extracting reagent (0.05N HCl in 0.025N H 2SO4) – Dilute 4 mL of concentrated
hydrochloric acid (HCl) and 0.7 mL concentrated sulphuric acid (H2SO4) into a 1000 mL volumetric
flask and bring to volume with deionized water.
F. EXPERIMENTAL PROCEDURES
1. Weigh 5.0 g air-dried 2 mm-sieved (2-mm) soil into a falcon tube.
2. Add 25 mL Mechlich No. 1 extraction reagent and shake for 5 min on a reciprocating shaker.
3. Immediately filter through Whatman No. 2 filter paper and save the extract for Fe, Mn, Cu and Zn
concentration determination.
G. RESULTS
Analysis of Micronutrients (Fe, Mn, Cu and Zn) required 5.0 g air-dried soil and 25 mL Mechlich No.
Result from ICP
Micronutrients mg/L
Fe 2.68
Mn 1.75
Cu 0.874
Zn 0.524
Calculation:
Convert value of micronutrients in mg/L to mg/kg (show calculation).
mg 2.68 mg 25 ml L 1000 g
i) Fe = = X X X
kg L 5.0 g 1000 mg kg
= 13.4mg Fe / kg
mg 1.75 mg 25 ml L 1000 g
ii) Mn = = X X X
kg L 5.0 g 1000 mg kg
= 8.75 mg Mn / kg
mg 0.874 mg 25 ml L 1000 g
iii) Cu = = X X X
kg L 5.0 g 1000 mg kg
= 4.37 mg Cu/ kg
mg 0.524 mg 25 ml L 1000 g
iv) Zn = = X X X
kg L 5.0 g 1000 mg kg
= 2.62 mg Zn / kg
H. DISCUSSION
1. State the status of micronutrients content in the soil sample (Refer Table: Range in Critical
Level for Micronutrients).
Micronutrients Range Result Rating

Fe 4.5 - 10.0 13.4 High
Mn 5 - 10 8.75 Medium
Cu 0.2 - 1.0 4.37 High
Zn 0.8 - 2.0 2.62 High
2. Briefly explain the reasons or factors influencing micronutrients content in the soil
sample.
1. soil properties : colour, texture, structure, porosity, density, consistency, aggregate

stability, and temperature are all physical qualities of soil and.
2. Parent material: This is where most soil growth begins. Mineral rock and/or organic
materials could be the parent material. Soil formation begins when parent rock
material is exposed to the atmosphere or organic matter and/or minerals are
deposited on the earth's surface.
3. pH: The acidity or alkalinity of the soil is measured by its pH. As a result, the hydrogen
ion concentration is low at high (alkaline) pH levels. The pH of most soils is between
3.5 and 10.
4. CEC: The entire capacity of a soil to hold exchangeable cations is known as cation
exchange capacity (CEC).
5. Carbon content of organic matter: - It is a component of soil organic matter that may
be measured. Organic matter takes up only 2–10% of most soils' mass, yet it plays a
critical role in agricultural soils' physical, chemical, and biological functions
3. Discuss the importance of micronutrients to the plants.
1. Osmotic and cation neutralisation are the main functions of Cl.
2. Plants use Cu, Fe, and Mo in oxidation-reduction reactions, which are necessary for
photosynthesis and other metabolic functions.
3. Fe is required for chlorophyll production and can be low in turf grass, particularly in alkaline
soils. Compaction, for example, can induce iron deficiency because it inhibits root growth.
4. Plants require Zn and Mn for specific N transformations.
5. Mo is required for symbiotic nitrogen fixation.
6. B is involved in the division of cells and the production of seeds.
I. CONCLUSION.
A proper crop nutrition management plan is essential for a healthy and productive crop.
Micronutrients are essential elements needed for crop growth that are required in small quantities.
Even though micronutrient requirements are low, these nutrients directly affect the growth and
development of crops. The importance of the role of trace elements for plants is as follows:
1. Plants' enzyme systems contain the majority of micronutrients.
2. Redox reactions rely heavily on micronutrients.
3. Micronutrients are essential for photosynthesis.
4. Micronutrients have a role in events including nitrogen fixation and protein
synthesis.
REFERENCES
1. https://blog.agrivi.com/post/the-extremely-important-role-of-micronutrients
2. https://www.researchgate.net/publication/344308663_Extractable_micronutrients_status_in_
relation_to_other_soil_properties_in_Billiri_Local_Government_Area
3. Lecture note.
4. Lecture Video.
- LABORATORY REPORT END –

Soil Fertility AGR521: Available Micronutrients

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SOIL FERTILITY

STUDENTS NAME & 1. MOHD HAFIZI BIN IBRAHIM

2. MUHAMAD SYAMSUNIZAM BIN MOHD ZAINI

LECTURER NAME ANISAH BINTI MOHAMMED

ACADEMIC SESSION MARCH – AUGUST 2022

1. Elements in primary and secondary minerals

2. Adsorbed to mineral and organic matter surfaces

3. Incorporated in organic matter and microorganisms

D. APPARATUS MATERIAL AND PROCEDURE

4. Mehlich No. I reagent (0.05N HCl in 0.025N H2SO4)

1. Weigh 5.0 g air-dried 2 mm-sieved (2-mm) soil into a falcon tube.

Result from ICP

Micronutrients Range Result Rating

1. soil properties : colour, texture, structure, porosity, density, consistency, aggregate

1. Osmotic and cation neutralisation are the main functions of Cl.

photosynthesis and other metabolic functions.

4. Plants require Zn and Mn for specific N transformations.

5. Mo is required for symbiotic nitrogen fixation.

6. B is involved in the division of cells and the production of seeds.

- LABORATORY REPORT END –

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