Scribd Logo
Upload

Welcome to Scribd!

  • 34 views

    Biology Term 2 Practicals - ATPRO

    Uploaded by

    Pratibha Gupta
    This document outlines procedures to test for the presence of sugar in urine samples using Benedict's solution and Fehling's solution. The procedures involve adding the urine sample to test tubes and then adding the reagent solutions, heating if using Benedict's solution, and observing for the formation of precipitates which would indicate the presence of sugar. Precautions are mentioned to carefully follow the procedures and use proper measurement tools.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Biology Term 2 Practicals - ATPRO

    Uploaded by

    Pratibha Gupta
    34 views12 pages
    This document outlines procedures to test for the presence of sugar in urine samples using Benedict's solution and Fehling's solution. The procedures involve adding the urine sample to test tubes and then adding the reagent solutions, heating if using Benedict's solution, and observing for the formation of precipitates which would indicate the presence of sugar. Precautions are mentioned to carefully follow the procedures and use proper measurement tools.

    Original Description:

    Praticle class 12

    Original Title

    Biology term 2 practicals.ATPRO

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document outlines procedures to test for the presence of sugar in urine samples using Benedict's solution and Fehling's solution. The procedures involve adding the urine sample to test tubes and then adding the reagent solutions, heating if using Benedict's solution, and observing for the formation of precipitates which would indicate the presence of sugar. Precautions are mentioned to carefully follow the procedures and use proper measurement tools.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views12 pages

    Biology Term 2 Practicals - ATPRO

    Uploaded by

    Pratibha Gupta
    This document outlines procedures to test for the presence of sugar in urine samples using Benedict's solution and Fehling's solution. The procedures involve adding the urine sample to test tubes and then adding the reagent solutions, heating if using Benedict's solution, and observing for the formation of precipitates which would indicate the presence of sugar. Precautions are mentioned to carefully follow the procedures and use proper measurement tools.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 12

    Expt. N o .

    3
    age No.

    OMechve To stucdy the distxibution cStomaa ona luaf.

    Reqincment: Lia ebuebhyllum o petuia (kecauk thuy a hickk


    and ik is Lakyto pl olayers ptom tham), Jescpb, atth qlass,
    hotriclishes biush, necdlek, kaplanin, aycini, lidles scalhsls,
    Combound micHoscope.

    PHoCeduL
    Sulace Mth 0ato
    Taka hualthy las and clian ut
    2Tako peelk pom ubhes and lsueu suiaces of Leop
    into duso dilhenert atch qlasaes uxth puepex
    3THansfë ihem
    Jabel Add sofxonin in tatel qlake8 lides,
    sepaxate maxk thm
    4Nouadxansehise oeels on too

    bMaperly stain unth


    hape. Remose
    5 Cut the pul in uctangulas and tanejuly
    ut a diusp of qlywine
    ihe hulb o i l t papen
    plate a coie ip.
    bQbsexve iheke Mides undex a micioscope clihexentiated. As the
    aselasily
    Epidenmal and tematal culls and malle in ize a s
    stornatal cells a kidnuy bean
    cals.
    Cembated t o spidumal bolh
    belh
    Momataand hideumalcells
    on

    Caunt_the numbex o
    widles. Calculate stomXial induoc.
    Numbex of Stomata x 10
    Stomatal Tadso Somalat Numhu of opiclmal
    cells
    Numbro

    Teacher's Signature
    EpideMmal
    Cal
    -Subsicliay ul
    Stom atal Gtuard cells
    Pete

    1licroscopic viw c Epicomis


    Date

    . No. Page No.

    O.bSeMvaten Numbex of Mlomata aue moteon louseX ulac; hente


    the Momaia indo the Jou MUlace ix highar bs companed
    Jo that othe po Muface..

    Rusults
    kea ace Numbeto Sfomata Numberof EpicloAmalSfomatal Lnde
    CotlS

    Uppex
    heuA

    PLcauion&
    Avaid the cunling o th peel.
    hal Hanin
    2Use a blotling pahe to omoue xcoss amaunt

    and9lycemine.
    3 Avei tho oue Maininq Leal and dont let il be o cly
    4 Sliclesheuld be cloand
    5 Count alls caxeJully
    t
    6 Do nstJet the fcel k

    Teacher's Signature
    pt. No

    Page No.

    O.bjeckiueSebatationoblant pigmants thxough pahex Chomafoguaphp


    Reauinements. Sbinach Jeaves, chxomatoqnaphy chamber motlox
    Ond pIstle, thex acatone boluent, scissotb,aceone, penil,
    captllarytube, patuula, scoale, iltn pabex Mübs, Ldatth
    glases thxead s.xaplan. a
    Prece.dusL
    1Takt 5q eshly gteen shi.nach Leaves tuut into malluors uing
    scissos ànd qind tham in a mota bestle ao aclding 5mL
    a.cctone in it AU the pigmants will com in the acetone
    Selution.
    2 filtethe slusy and discand_the
    theMabiclues
    3Take a stuip o liox pabr houing a nane notch at one end
    t h e Mrip
    4Take a bencil and a cal and clrad a hoizontal line uwth
    a benil akout 23cm alulay tom the tih o th netch.
    5 Put adep o the pigmont octxact in the micdle othe
    line usdh the help o a Capillaup ube
    6Alled_the cduop_to edy and xapent till fsu e ive deops axe
    placed on the bapox.
    Takethe chKomatoqaphic chambtl ondpout Jthet occtone
    holuent_in it
    8 Feld one end cf the iltr papetMrip ond stable it.
    9Uing a thxead, Aang the ltx babex uib in the chxamataqtap
    i c chambex

    Teacher's Signature
    Chtomalogtabhy fabe
    (Stahondry phave)

    Piamants Coneena
    Sebaealud
    onthoph
    Chla
    Selijent
    (molbile pha) Chlb

    Spof of piqmants o
    to besebototud un

    topex chvomatogtaphy ok plant (Lunes) Pigments.

    undo
    Expt. No.
    Page No.

    JoThleading shet should umain_abeut Lcm aboue th soluent louel


    i Leaue the chxomatogn.aphic chom.bex
    12 Le can cbSexe as th seluent maues unclistumhed to ome ime
    thxough the pabex, il
    pHLadsiho cliloxont þigmonts o the mixtu io Vaticus dlistancus.
    18 han_ tho oluent iS above 34
    thu the Sbup, emoue tha
    sbup caakully ond lat it_cu
    Obsaxuafion.Oileont pigmants cbaxate at clihexont Jauels on
    liex stiu (U
    Result:

    S. Na Pigmant istonce o the spet ftom Oistance o the solutR ab


    0iginal une a) in cm nom oigindl lind 6) in cm_

    :Chletophyll b
    2 chloxophula
    3 Xanlhophyll
    4 Caxotune

    Precauhons:
    Spinach Jeaues Should ke sh oncd qxaur
    The londing spet Ahold ke 2-3cmaday em theip o th netch.
    3 hile hanginq the Mupsin the chxomalociabhuy chambex, ihi
    loacling Mao hould sxamaio about Lem abeve`he oluentJunel

    Teacher's Signature
    pt. No. 5 Page No.

    Objechive: Ta study and Comment o n thi ecpeximntal set-up fe


    acHGlic H&bixation.

    Reiements SoaReel beccls pn o qptom, conical lask,a tst


    tube beakex, KOH andstancl

    PMoCocluL

    TaRz abouf 30 qHompua sendsin a ptxi clish and allout them in


    Quminat by umbibing iham
    Ohce he gninahion sthuts, tuonsJex them in o conical lask.
    Tak 4ml o ptaium huydseride (KOH) seluhion into a tule and hang
    into ths cönical lask usith the hetb of a thxead abeue the
    quuminaing seads
    Susbend _test tulbe in the conical lak akee the quuminahing
    esds

    5 Close the mouth o the cenical lask toith a cerk


    ene ndof a dalivexy tulè into he conical ask thneuqh
    | Lnert lime twatou.
    the terk and clib the sther in a kakx containing
    en thi cetk_to make Bhe aphaxatus
    A
    iApltha pibuelun jelly
    aiNtighf
    down the þesition initial wate luel in bent qlak kl
    8- Note
    Ato u hous, theleuel of uwatex uises in the glass
    Obkeruahon
    th Co2 weluecd cluxing aerehic
    tube Tt i because,
    will be absorbedby KoH
    spinotion Jy qpxminahing eecs
    usill uise in thu glas tube..
    soluhonand tke auel of_Satax
    KaCO3 t H,0
    Cot 2koH
    Teacher's Signature -
    Potasium fajclrozide-

    Conical lask- Watex

    Respiing Seeds-
    Resulth The uie in auel of uWatox
    Mhgws aBic aspixahon un
    QLxminahng Aends
    S.Na Inihal Jouelaf watux (o) final lawel o watox (b) Rise in the level o
    wat (o-b)
    2

    PHLcauhons:
    1 the cotk pueperly do make Conical lask ait tight.
    2 Kasp quxminaing seods melst
    3KOHaeluhoniikupt to uplenish OxuQeninkide conical lask
    Expt. No. 6 Page No.

    Objeche: To Mudu the Dxa&ence oc Sugax in UuineBlooc


    Sample VN

    Reauutemont's Test tube tost tulee heldoH,UineAamble mLasutin


    Culinclos, Bonedticts holuhion fehlinqu Seluhion buxnu

    Peteczdute(Benaclirt's Methocd
    TaR 2mL wine sam.bl in a mLa&uting ulindextom the uwnin
    bombl bottle
    2 TaRe a tost Tulbe and pout Utihe komplin it
    3Tak 5m Beneclicts_HLagent un a measwing Cylincdot.
    4 Acld Beneclict Jeagehtto the ust tube hat contains
    wHine omble.
    5 Uhing a test tuhe holder, hold tho tst tube itmly and huat
    it Yo2 minutts enthe bwno
    6Keup shaRing the bst hube _while hoating
    Aellou pcipitate aphe.au hich indicatus the busence of
    ugounutin.
    8Dapending upon the COncenDHahon of ugaH in tht uun eithu
    gLn yellono, o oucR ed puacipitatsatefotmed
    PatecedueFEHL NG's TEST)

    1TaRe 2ml wine ample dn a


    mLakuungCylindeM omthe
    Jstine ampl Jbottl.
    2 1ake a iust tube and bou the wine homple. in il.

    Teacher's Signature
    Date
    Expt. No.
    Page No.

    3 Tak 2ml Fehling'k Seluhon A in a masuinq


    Clinclex
    4
    4| Adld Fehling's Seluion do the tet tuke tht tontaint unin sambl
    5 Take 2mL fehling
    seluhon 8jn a meakutingube
    6Add2mL Fehlinoluhon do the tust tube that coentains
    utina omple
    Usinq a iest tube helclo, held the fust tube tomly oncl haat
    it qendlyJe 2 minuds on dhe burnen
    88 Kaup
    Krp shaking the test tule Shile huating
    hoafing
    9A teen -pcibiatr_ abboons ushichindicates the pusence of
    Hacso Suqayinuint.
    10Debthdling uupon thetonceniation of uga inthe umine, eithu
    een alleu o buick_Aid pucipitates ak teimd.
    O.b8MVations: Deþending upon dhe toncuntration o qlucose, q n
    yellors and Jsick d pucipilates ofcubHous 0xide aL oumad.
    Buler is the talbl sheueing the coloux cquonce depending
    upon ih conconirahon o qlucoselauel.

    Celau ol ftocipiiat Pencentage oSugapsátnt


    Blue Suga absent
    Gueen 0.5 to L
    Yellaud 1to 2e Sugax
    Brick ud 2k 01 me SuqaH

    Teacher's Signature
    Expt. No.
    Page No.

    Ptucouion

    1 Wash a0lthe test tubes kelene and alto use


    2 Reagonts shauld he Lwsh fon his tost to
    qet cect obsexua.ions
    3 0se tst ube heldons to heat he tuc onbwine,
    4 Ue sepaxatr duobbets to each sluhion
    5 Weau qtonR hile LoeRing with xLagents and bampl

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy