Note

Iodometric Determination of Hydrogen Peroxide

Titration Application

Introduction Sample Preparation and Procedures

Titration Application

Hydrogen peroxide (H2O2) is a strong oxidant.  Add 40 mL deionized water into the
It has the benefit of being able to oxidize titration beaker.
organic substances while decomposing into  Weigh in approx. 0.4 g of aq. hydrogen
harmless water (H2O). peroxide solution.
 Add 2 drops of molybdate catalyst using
Low concentrated aqueous solutions a Pasteur pipette and place the beakers
(e.g. 3%) are widely used as disinfectant e.g. on the InMotion rack
in dentistry, in hand sanitizers and for contact  Start method M860
lenses. Higher concentrated solutions are Note: Potassium iodide and sulfuric acid are
Note

used in the food and beverage industry to automatically added via pumps.
clean production lines (e.g. PET bottles) or for
water treatment. Chemistry
Preparation
Herein we describe a titration method to H2O2 + 2 KI + H2SO4 → I2 + K2SO4 + 2 H2O
determine the hydrogen peroxide content in Titration
aqueous solutions. With the help of a I2 + 2 Na2S2O3 → Na2S4O6 + 2 NaI
molybdate catalyst, hydrogen peroxide
oxidizes iodide to iodine that is subsequently Solutions and Chemicals
titrated with a 0.1 mol/L aq. sodium thiosulfate Titrant: aq. Na2S2O3, c = 0.1 mol/L.
solution. Sample: Hydrogen peroxide in water
(approx. 3% w/w).
This method serves as an alternative to the Analyte: Hydrogen peroxide, H2O2,
direct titration of hydrogen peroxide with M = 34.01 g/mol, z = 2.
potassium permanganate (M410). The Standard: Potassium iodate, KIO3,
iodometric method is more time consuming M = 213.99 g/mol.
and requires more additional equipment and Additional reagents: Aq. potassium iodide
chemicals. However, as the molybdate- solution (KI, 1 M); aq. sulfuric acid solution
catalyzed generation of iodine is very specific, (H2SO4, 25% v/v); aq. ammonium molybdate
it is less susceptible to interferences giving solution ((NH4)6Mo7O24, dissolve 9 g in 25 mL
more accurate results in some cases. dest. Water); distilled water.

Instruments and Accessories

 Excellence Titrator T5 (30252672), T7
(30252675) or T9 (30252676)
 Burette 10 mL DV1010 (51107501)
 2 SP280 pumps (30094237)
 DMi140-SC platinum ring electrode
(51109520)
 XPR205DR Analytical balance
(30355415)
 InMotion Flex 100 mL autosampler

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M860 v1.0
 Results Hydrogen peroxide is quite reactive. In order
The hydrogen peroxide content was to prevent it from reacting with dirt and
Titration Application

determined as 2.67(6)% with a precision of organic residues in the empty titration beaker,
srel = 2.0%. deionized water is added prior to weighing in
the sample.
H2O2 content [% w/w]
1 2.765
Waste Disposal and Safety Measures
After the titration, discard the content of the
2 2.688
beakers into the aq. acidic waste.
3 2.620
4 2.649
Wear safety goggles, a lab coat and gloves
5 2.617 during the sample preparation, titration and
6 2.688 waste disposal.
Mean 2.67
s Further Information
Note

0.06
srel 2.0% https://www.mt.com/us/en/home/products/La
boratory_Analytics_Browse/Product_Family_
Remarks Browse_titrators_main/Titration_Excellence.
The titer of 0.1 mol/L aq. sodium thiosulfate html
was determined using potassium iodate as
the primary standard according to method
M009.

With minor modifications of the method and

manual addition of reagents, the titration can
be performed using a G20S Compact titrator
(30252669).

As the iodide solution formed is not very

stable, the addition of potassium iodide and
sulfuric acid needs to be done sample by
sample. Within this method, KI and H2SO4
are added automatically via pumps just
before the titration starts.

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M860 v1.0
 Measured Data
Titration Application
Note

Titration curve and first derivative of the titration curve of sample 1/6.

Volume dV/dt Potential dE/dV Time

[mL] [mL/s] [mV] [mV/mL] [s]
0 NaN 389.2 NaN 0
0.01 0.0035 389.4 NaN 3
0.02 0.003 389.3 NaN 6
0.045 0.008 389 NaN 9
0.1075 0.0195 389.1 NaN 12
… … … …
6.766 0.0255 322.4 -324.65 117
6.797 0.01 315.8 -638.71 120
6.813 0.005 310.8 -1013.57 123
6.8285 0.005 302.7 -1170.28 126
6.8385 0.003 291.8 -1334.94 129
6.841069 EQP 276.6 EQP EQP
6.8485 0.001 232.5 -1009.49 140
6.8585 0.001 211 -1172.85 149
6.8685 0.001 203.4 -936.34 158
6.8935 0.004 196.2 NaN 164
6.956 0.01 190.1 NaN 170
7.112 0.0255 184.2 NaN 176
7.312 0.041 180.2 NaN 181
7.512 0.0525 177.4 NaN 185

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M860 v1.0
 Method M860 At slope No
001 Title After number of EQPs 1
Titration Application

Type General Titration Combined termination criteria No

Compatible with T9, T90, T7, T70, T5,
T50 008 Calculation R1
ID M860 Result Content
Title Iodometric Result unit %
Determination of Formula R1= Q*C/m
Hydrogen Peroxide Constant C=M/(10*z)
M M[Hydrogen peroxide]
002 Sample Decimal places 3
Sample Type Sample Result limits No
Number of IDs 1 Extra statistical func. No
ID1 Hydrogen peroxide Send to buffer No
solution Condition No
Entry type Weight
Lower limit 0g
Higher limit 5g 009 Rinse
Density 1 g/mL Aux. reagent Water
Note

Correction factor 1.0 Rinse cycles 1

Temperature 25.0°C Vol. per cycle 10 mL
Entry Arbitrary Position Current position
InMotion reader None Drain No
Number of sample factors 0
010 End of Sample
003 Titration stand
Type InMotion T/Tower A
Titration stand InMotion T/1A 011 Record
Head position Sample Report template Titration report
Print no
004 Pump
Aux. reagent KI
Volume 5 mL
Pump property 1-way

005 Pump
Aux. reagent H2SO2
Volume 5 mL
Pump property 1-way

006 Stir
Speed 30%
Duration 60 s

007 Titration (EQP) [1]

Titrant
Titrant Na2S2O3
Concentration 0.1 mol/L
Sensor
Type mV
Sensor DM140-SC
Unit mV
Temperature Acquisition No
Stir
Speed 30%
Predispense
Mode None
Wait time 0s
Control
Control User
Titrant addition Dynamic
dE (set value) 7 mV
dV(min) 0.01 mL
dV (max) 0.2 mL
Mode Equilibrium controlled
dE 1 mV
dt 1s
t(min) 3s
t(max) 10 s
Termination
At Vmax 10 mL
At potential No

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M860 v1.0