Laboratory Tools
Laboratory Tools
REAGENTS
A reagent is an integral part of any chemical reaction. A reagent is a
substance or compound that can facilitate a reaction, and they are used in
most widely used tests. This includes, for example, pregnancy tests, blood
glucose tests, and most COVID-19 test kits.
LABORATORY RULES
A standard list of basic laboratory safety rules are given below, and must be followed in
every laboratory that uses hazardous materials or processes. These basic rules provide
behavior, hygiene, and safety information to avoid accidents in the laboratory.
Laboratory specific safety rules may be required for specific processes, equipment, and
materials, which should be addressed by laboratory specific SOPs.
Basic Safety Rules
Basic safety rules for laboratory conduct should be observed whenever working in a
laboratory. Many of the most common safety rules are listed below.
Depending upon the scientific research being conducted, a lab can be filled with
dangerous chemicals, radioactive substances, biological specimens, sharp
instruments, breakable glassware, and flammable objects. Thus, those working in
labs need to be keenly aware of the many dangers associated with these items. In
order to maintain a safe workplace and avoid accidents, lab safety symbols and signs
need to be posted throughout the workplace. Researchers, staff, and visitors should
note and understand the hazard communication information on the laboratory
safety signs, including specific hazardous agents (biological, chemical, radiological),
physical hazards (lasers, magnetic fields) present in the space, stated precautions (no
food or drink allowed), and required personal protective equipment (lab coats, eye
protection, gloves, etc.).
The following laboratory safety symbols warn of possible dangers in the laboratory
to help lab professionals keep safe and informed.
Look for the gloves safety symbol to identify when hand protection should be worn
for handling hazardous materials, even in small quantities. It is important to choose
the appropriate type of glove for the hazard present, such as chemical resistant
gloves, heat resistant gloves, etc. Be aware that no chemical resistant glove protects
against all chemical hazards. Read the Material Safety Data Sheet for guidance on
the appropriate type of glove to wear, or consult with lab supply distributors for
glove vs. chemical comparison charts when choosing chemically resistant gloves. Be
aware that some materials may cause reactions in some workers such as allergies to
latex. Make sure the gloves fit properly.
2. Boots Required
The laboratory boots required safety symbol indicates when street shoes are not
adequate for certain lab-related tasks. Chemical resistant overshoes or boots should
be used to avoid possible exposure to corrosive chemicals or large quantities of
solvents or water that might penetrate normal footwear. Leather shoes tend to
absorb chemicals and may have to be discarded if contaminated with a hazardous
material. In a lab, dropping a beaker of acid will soon destroy an ordinary pair of
shoes. Specialized laboratory footwear is designed for specific applications and
settings.
3. Protective Clothing
The protective clothing safety symbol indicates that a lab coat or other protective
clothing needs to be worn. There are several types of lab coats for different types of
protection. Cotton protects against flying objects, sharp or rough edges, and is
usually treated with a fire retardant. Since many synthetic fabrics can adhere to skin
when burning, cotton is the most preferred laboratory clothing fabric. Wool protects
against splashes of molten materials, small quantities of acid, and small flames.
Synthetic fibers protect against sparks and infrared or ultraviolet radiation.
Aluminized and reflective clothing protect against radiant heat.
4. Safety Glasses
The eye protection safety sign indicates there is the possibility of chemical,
environmental, radiological, or mechanical irritants and hazards in the laboratory.
Eyeshields, also called safety glasses, goggles, or spectacles, not only provide
protection against flying debris and chemical splashes in the lab, but may also
protect against visible and near visible light or radiation from UV rays, depending on
the lens material. The most popular lens material for lab safety eyewear is
polycarbonate. This material has less than half the weight of glass, making the
eyewear more comfortable to wear. Modern eyeshield designs offer anti-slip nose
bridges, anti-fog lenses, and coatings, which make them resistant to acids, caustics,
and hydrocarbons.
5. Breathing Masks
Respirators are designed to prevent contaminated air from entering the body. “Half
mask” respirators cover just the nose and mouth; “full face” respirators cover the
entire face; and “hood” or “helmet” style respirators cover the entire head.
Respirators can protect the user in two ways: By cleaning the “dirty” outside air that
passes through a filter or adsorption bed or both when one inhales; or by supplying
clean breathing air from a remote source. The clean air can either be delivered via a
supply line, or the clean air is packaged and carried with you in a tank. The breathing
mask safety sign lets you know that you’re working in an area with potentially
contaminated air.
6. Face Shields
The face protection safety symbol lets lab personnel know that a large face shield,
similar to the glass shield on a motorcycle helmet, must be worn when executing
experiments that carry the potential of causing an explosion inside of the hood. Full
face (and possibly throat) protection from splash and/or impact is commonly
required for work on or in the presence of human pathogens, some laboratory
chemicals, explosion hazards, heavy grinding and heavy spraying or splashing,
and with large (2 L and larger) quantities of dangerous liquids such as acids, bases,
and many organic liquids. A face shield can also afford extra protection against
extreme temperatures.
7. Hearing Protection
Noise in laboratories has become a growing concern. While the noise levels in most
laboratories are below the threshold level that damages hearing, laboratory noise
can be fairly loud. The operation of large analyzers (e.g., chemistry analyzer), fume
hoods, biosafety cabinets, incubators, cell washers, tissue homogenizers, and stirrer
motors, all contribute to the noise level. There is a wide variety of hearing protection
devices available. Different devices are designed to protect against different
severities of noise. The ear protection safety symbol indicates that lab workers are in
a dangerously high decibel noise range.
9. Safety Shower
Safety showers need to be installed in all areas where laboratory employees may be
exposed to splashes or spills of materials that may be injurious to the eyes and body.
As a general rule, new shower installations should adhere to the recommendations
for shower location and minimum performance requirements established in
American National Standard Z-358.1 (1998). Showers should be placed as close to
the hazard as possible, but in no case more than 10 seconds' travel time from the
hazard. Safety shower signage should be prominently displayed close to the shower.
A no food and drink safety sign lets lab personnel know that eating and/or drinking
where hazardous materials are used, handled, or stored is not permitted, as such
activity can result in the accidental ingestion of hazardous materials (chemical,
biological, and/or radiological). Food or beverage containers may not be stored in
the laboratory and washed drinking cups, food containers, or eating utensils may not
be dried on laboratory drying racks. Refrigerators used for storage of research
materials must not be used for storage of food or beverages.
The no pacemakers or metallic implants lab safety sign is used to warn lab staff of a
strong magnetic field hazard. This is caused by lab instruments that use
superconducting magnets such as nuclear magnetic resonance spectrometers
(NMR). Staff with pacemakers or metallic implants cannot work in such areas of the
lab and other staff should take care to remove metal objects such as jewellery,
watches, loose change, etc. before entering these areas, in order to prevent injuries.
Fires can happen anywhere, but lab fires can be even more dangerous due to Bunsen
burners, flammable liquids, research documents, laptops, and lab equipment that
might be present at any given time. Due to these unique circumstances, it’s
important that every lab be prepared with the correct fire extinguisher, inspection
requirements, and training. It is essential that the occupants of a laboratory are fully
aware of the risks and the appropriate extinguishing media. A fire extinguisher safety
sign indicates the exact location of a lab’s fire extinguisher.
The fire blanket safety sign indicates where a fire blanket is located in the lab.
Housed in a case or not, the woolen blanket is used for smothering fires and for
containing and controlling chemical spills.
If someone in the lab should catch fire, he or she should get on the ground and start
rolling to extinguish the flames. Lab personnel can assist by using the blanket to
speed extinguishing the fire, preventing further injury. Fire blankets are large enough
to entirely cover most people, depriving the fire of the oxygen that it needs to
continue burning.
Bunsen burners, lighters, matches, and any other flame-producing devices are
considered “open flame devices.” Open flame devices carry with them the risk of
unintentional fire and serious consequences when not used appropriately. Most
organic chemicals are flammable. The chance of a fire is substantially increased
when open flames are present. No open flames safety signage indicates to lab
personnel the risk and prohibition of open flame devices.
The first aid station safety symbol indicates the availability of a first aid kit. The kit
should be easily accessible to other lab locations that belong to a particular group.
The first aid kit should contain the items recommended in the First Aid Kit Policy and
Guidelines for Laboratories. It should be inspected monthly to ensure that no items
are missing and that none of the remedies (e.g., saline solution, ointment) in the kit
have expired.
The emergency meeting point sign marks a safe place, either inside or outside the
building, where laboratory employees should meet in the event of an actual
emergency (chemical spill, fire, etc.) or emergency drill. In their safety training,
employees should be made aware of the location of the emergency point, or the
meeting point closest to their location if there are multiple meeting points. Such
locations should be large enough to accommodate all employees in the event of an
evacuation. It’s always a good idea to have a backup meeting point in case the
primary one has been destroyed or is inaccessible due to the emergency taking
place.
Hazard Symbols
20. General Warning
The general warning lab safety symbol consists of a black exclamation point in a
yellow triangle. As you’d expect, it is a general warning to laboratory staff that a
hazard exists. This symbol can be found on equipment, doorways, cupboards or
other areas of the lab. It provides a good reminder to work safely and check if you
are not sure of the safety procedures for certain equipment or areas in the lab.
The health hazard sign denotes chemicals in the lab that can cause serious, often
long-term health problems. Hazards include carcinogens, respiratory sensitizers,
reproductive toxins, aspiration toxins, target organ toxins, and mutagens. An
important step in protecting worker health is recognizing the various health hazards
in the lab, as ignorance of the harmful effects of laboratory materials can have
serious and even fatal consequences.
22. Biohazard
The biohazard lab safety sign warns of lab equipment such as fridges or freezers that
either contains biohazardous materials or could be contaminated with biohazardous
material such as blood samples. This sign also marks entire areas of the lab that
either contain or are exposed to biohazards, for example, a lab working with
infectious agents. Workers should always wear the proper PPE and follow proper
procedures when working with such agents. Managers should also have an effective
exposure control plan in place in case of an emergency. Regular cleaning and
decontamination of areas and equipment that are exposed to biohazards is also a
must.
Substances labelled with the irritant symbol are not corrosive, but they can cause
discomfort and reddening, irritation, or blistering of the skin. Laboratory workers
should handle irritants carefully. Working with irritants in the fume hood can help
individuals avoid inhalation. Examples of irritant substances include ammonia,
chloroform, and chlorine.
The toxic material symbol indicates the presence of substances that may harm an
individual if they enter the body. Possible routes of exposure to toxic materials are
through inhalation, skin contact, and ingestion. The hazards and health effects
associated with toxic materials depend on the specific material in question, the route
of exposure, and the concentration of the material.
The high voltage warning symbol, which includes a lightning bolt arrow in a yellow
triangle, warns of voltage high enough to cause serious injury or death. In general,
you should stay away from equipment or areas of the lab marked with this symbol,
though if you do need to work close to such hazards, protective clothing and rubber
gloves should be worn.
The electrical hazard safety symbol, which typically includes a frayed wire and a hand
with a lightning bolt across it, indicates any electrical hazards in the lab. Such hazards
can cause anything from a mild tingling to death. With direct current, a person can
detect a "tingling" feeling at 1 mA and the median "let-go" threshold (the current at
which one cannot release the conductor) is 76 mA. For 60 Hertz alternating current,
the values are 0.4 mA and 16 mA, respectively. If an electrical hazard is suspected,
the device in question should be disconnected immediately and the cause
determined by a qualified technician. Equipment should always be turned off and
unplugged when any work is being done on it.
30. Laser Beam Hazard
The laser beam hazard sign in the laboratory lets staff know that hazards from laser
beams are present. Both the eyes and skin can be damaged from direct exposure to
laser beams so proper eye protection and non-flammable clothing should always be
worn at all times in these areas of the lab, whether the laser is being operated or
not.
The UV light hazard symbol will appear near ultraviolet (UV) light areas to warn
personnel of the potential dangers. UV light is a type of electromagnetic radiation
with a wavelength between 180 and 400 nm, which is shorter than those of visible
light, but longer than those of X-rays. Health effects due to acute exposure to UV
light include redness or ulcerations on the skin, while chronic exposure can lead to
skin cancer.
As you’d expect, the hot surface safety sign warns laboratory employees of burn
hazards from hot surfaces. Such hazards can come from lab equipment such as lab
ovens and autoclaves or from the building itself—if there are steam pipes, etc. Lab
workers should avoid touching such surfaces, but if they could come into contact
with hot surfaces during their daily work or must handle hot equipment, they should
first be trained on how to properly use such equipment and always wear the correct
PPE (ex. heat-resistant gloves, lab coats, eye protection, and closed-toed shoes).
The low temperature warning symbol warns staff of low temperature or cryogenic
hazards in the lab. Such temperatures are usually much lower than freezing point
and can be found in cold storage areas of the lab or where chemicals such as liquid
nitrogen are stored. PPE for working with cryogenic or low temperature hazards may
include: gloves designed for this purpose (thick and made from rubber and cloth,
covering the skin up to the elbow), long pants, closed-toe shoes or boots, a rubber
apron, and face shield.
The ionizing radiation hazard safety sign indicates the presence of ionizing radiation.
Ionizing radiation is radiation that carries enough energy to liberate electrons from
atoms or molecules, thereby ionizing them. Sources of ionizing radiation in the lab
include X-ray apparatus, medical beam cannons, and particle accelerators. Any
laboratory possessing or using radioactive isotopes must be licensed by the Nuclear
Regulatory Commission (NRC) and/or by a state agency that has been approved by
the NRC, 10 CFR 31.11 and 10 CFR 35.12. Labs must limit entry of radionuclides into
the human body to quantities as low as reasonably achievable and always within the
established limits, and limit exposure to external radiation to levels that are within
established dose limits and as far below these limits as is reasonably achievable.
Optical radiation is another term for light, covering ultraviolet (UV) radiation, visible
light, and infrared radiation. The optical radiation hazard safety signs warns of
equipment that produces this type of radiation in the lab, such as high-powered
lasers. When working with such hazards, proper eyewear should always be worn. For
lasers in particular, proper SOPs should be in place, the beam path for the laser
system should be enclosed, and users should perform a physical survey for any
unwanted reflections before using the laser.
Other Symbols
39. Dangerous to the Environment
This symbol indicates that the substance in question can cause damage to the
environment, and is most often used to label chemicals that are toxic to aquatic
wildlife. Environmental hazards are classified as either acute or chronic. Lab workers
should dispose of environmentally hazardous materials properly, and ensure that
they do not get washed down the drain.
The symbol for oxidizing materials indicates the presence of chemicals that readily
give off oxygen or other oxidizing substances. Oxidizing materials may intensify fires
and cause explosions, and also may be toxic or corrosive. Although air is the usual
source of oxygen for burning, oxidizing materials can support a fire even in the
absence of air. Some common oxidizing liquids and solids found in laboratories are
bromine, chlorates, nitrates, perchloric acid, and peroxides.
The flammable gas symbol represents gases that will burn or explode if mixed with
air, oxygen, or other , in the presence of a source of ignition. Flammable gases used
in laboratories include ethyl chloride, aerosols, and liquefied petroleum gas.
Compressed flammable gases are common in many labs, and they are extremely
dangerous to work with and around.
Certain pieces of laboratory equipment generate strong magnetic fields. The strong
magnetic field sign alerts lab members to the dangers that this type of equipment
can pose. The risks are especially imminent for people wearing pacemakers and
implants, which will tend to align themselves with the magnetic field lines, as will
watches, clipboards, and certain tools.
The internationally recognized recycling symbol is formed of three arrows that point
in a never-ending loop. The arrows form a triangle shape. The recycling sign is used
in labs to indicate where recyclable items are gathered and sorted. Such items can
include any plastic that is not characterized as a biohazard or radioactive hazard,
Types 1 and 2 laboratory plastics, gloves, pipette tip boxes, Styrofoam containers,
cardboard—all of these are readily recyclable in most lab facilities. Ask your EH&S or
Facility Management representative about recycling in your facility.