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Effect of Temperature in Enzyme Activity

The document summarizes an experiment that investigated the effect of temperature on enzyme activity. Gelatin-stained rags were placed in detergent solutions at room temperature (24C), warm temperature (40C), and hot temperature (70C). The enzyme activity was measured by the amount of gelatin removed from the rags. The results showed that the warm temperature of 40C had the highest enzyme activity, while room temperature had the lowest. This indicates that enzymes have an optimal temperature at which they work best. Too low or too high of temperatures decrease enzyme activity, as temperatures can cause enzymes to denature. In conclusion, the hypothesis that higher temperatures would increase enzyme activity was not supported, as 40C proved to be the

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Juan Martín Villazón
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56 views

Effect of Temperature in Enzyme Activity

The document summarizes an experiment that investigated the effect of temperature on enzyme activity. Gelatin-stained rags were placed in detergent solutions at room temperature (24C), warm temperature (40C), and hot temperature (70C). The enzyme activity was measured by the amount of gelatin removed from the rags. The results showed that the warm temperature of 40C had the highest enzyme activity, while room temperature had the lowest. This indicates that enzymes have an optimal temperature at which they work best. Too low or too high of temperatures decrease enzyme activity, as temperatures can cause enzymes to denature. In conclusion, the hypothesis that higher temperatures would increase enzyme activity was not supported, as 40C proved to be the

Uploaded by

Juan Martín Villazón
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Juan Martin Villazón 11A

Effect of temperature in Enzyme Activity

Introduction

Enzymes are organic catalysts substances that speed up chemical reactions


without itself undergoing a permanent chemical change in the process. Enzymes
are specific for one particular reaction or group of related reaction. The enzyme
activity is affected by temperature and pH; sometimes these factors may cause
denaturation. Detergent, textiles, food and beverages, among other daily life
products contain enzymes. This lab about the effect of temperature in enzyme
activity. The primary goal of this experiment is to analyze the effect of temperature
in enzyme activity, by staining some rags with gelatin, the putting them into
detergent solutions at different temperatures and see which one got more gelatin
out. Based on this the hypothesis would be: If the temperature is related to the
enzyme activity then at higher temperatures the enzyme activity will be higher. The
reasoning that leads to this hypothesis is that high temperatures means there is
more energy and with more energy maybe enzyme work faster.

Materials and Method

Independent Variables Temperature: Measured


with thermometer.
Dependent Variables Enzyme activity: Amount
of gelatin removed from
the rags.
Constants Type and amount of
water and detergent,
container, type of rags
used, time that the rags
were left in the solution,
type and amount of
gelatin used in the rags

Materials

 Thermometer
 Rags (15 strips of 10cm divided in 5 spaces of 2cm)
 Beakers (3 minimum, can be used for making the gelatin solution and then
the detergent solution)
 Water (300 ml per beaker)
 Gelatin (22 g in each solution)
 Detergent (20 g in each solution)
 Chronometer
Juan Martin Villazón 11A

Method

1. Prepare the gelatin solution.


2. Introduce the rags in the solution and let them absorb the solution until they
are fully stained.
3. Take the rags out and let them dry.
4. Prepare the detergent solution in three different beakers.
5. Heat two of the beakers until they reach temperatures of 40 C and 70 C.
6. Introduce 5 rags in each of the three beakers with the detergent solution
and let them rest form 5 minutes.
7. Analyze how many of the 2 cm spaces have faded in each strip of rag to
calculate the percentage of the enzyme activity regarding the amount of
gelatin the removed from the rags.

Results

Table 1. Temperature Vs Enzyme activity.

Temperature ( Degrees Celsius C )


Trials 24 (room) 40 (warm) 70 (hot)
Enzyme 1 25 95 68
Activity (%) 2 50 90 54
3 22 82 62
4 35 78 51
5 40 95 63

The table shows that warm temperature had a better enzyme activity.

Graph 1. Effect of temperature in enzyme activity

Effect of temperature in en-


100
zyme activity
Enzymee Activity (%)

80
24 C
60 40 C
40 70 C
20
0
1 2 3 4 5
Trials per Temperature (Degrees celcius C)

The graph show that the highest enzyme activity was at 40 C and the lowest at 24
C.
Juan Martin Villazón 11A

Table 2. Average

Temperature ( Degrees Celsius C )


24 40 (warm) 70 (hot)
(room)
Enzyme 34,4 88 59,6
Activity
(%)

Graph 2.

Effect of temperature in enzyme ac-


tivity
100
90
Enzymee Activity (%)

80
70
60 Averages
50
40
30
20
10
0
24 40 70
Temperature (Degrees celcius C)

The graph show that in average the best temperature is 40 C and the least
effective is 24 C.

Discussion

Based on the table and the graph it is evident that the findings in the results do not
support the hypothesis. This could be due to the fact that enzymes have an optimal
temperature in which they work better; based on the results of the experiment we
can say that the optimal temperature is 40 C. The fact that at lower temperatures
the enzyme activity I the lowest can be because they do not have enough energy
to get activated, and at high temperatures they start decreasing their activity
because the star to denaturate.
Juan Martin Villazón 11A

Conclusion

The purpose of the experiment was to investigate the effect of temperature on the
enzyme activity by comparing how much the enzymes faded 15 gelatin stained
strips of rags at different temperatures with a control (room temperature). There
was a significant difference between the three temperatures, as the graph shows
above, being 40 C the optimal temperature for enzyme activity and 24 C the least
effective. The hypothesis that high temperatures would have a better enzyme
activity was not supported by the data. The experiment relied on a very inaccurate
way measuring the enzyme activity, which was comparing how much the gelatin
faded from the rags. The experiment could improve using another way of
measuring the enzyme activity or improving the one used so it is more accurate.
Additional investigation using different detergents could be done or with a different
source of staining.

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