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    Microbiotricks - Mme

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    siddhanta
    The document lists various types of media used for culturing and identifying different bacteria. It includes basal media like peptone water and nutrient broth as well as enriched, selective, differential and indicator media like blood agar, MacConkey agar, and chocolate agar. It also provides information on the optimal pH and preparation methods for each medium, including autoclaving. Finally, it summarizes the characteristics of several common bacterial species and the appropriate media for isolating and identifying each.

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    Microbiotricks - Mme

    Uploaded by

    siddhanta
    69 views21 pages
    The document lists various types of media used for culturing and identifying different bacteria. It includes basal media like peptone water and nutrient broth as well as enriched, selective, differential and indicator media like blood agar, MacConkey agar, and chocolate agar. It also provides information on the optimal pH and preparation methods for each medium, including autoclaving. Finally, it summarizes the characteristics of several common bacterial species and the appropriate media for isolating and identifying each.

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    MICROBIOTRICKS - MME

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    The document lists various types of media used for culturing and identifying different bacteria. It includes basal media like peptone water and nutrient broth as well as enriched, selective, differential and indicator media like blood agar, MacConkey agar, and chocolate agar. It also provides information on the optimal pH and preparation methods for each medium, including autoclaving. Finally, it summarizes the characteristics of several common bacterial species and the appropriate media for isolating and identifying each.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    69 views21 pages

    Microbiotricks - Mme

    Uploaded by

    siddhanta
    The document lists various types of media used for culturing and identifying different bacteria. It includes basal media like peptone water and nutrient broth as well as enriched, selective, differential and indicator media like blood agar, MacConkey agar, and chocolate agar. It also provides information on the optimal pH and preparation methods for each medium, including autoclaving. Finally, it summarizes the characteristics of several common bacterial species and the appropriate media for isolating and identifying each.

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    Peptone water Nutrient Broth Nutrient agar Blood agar MacConkey agar Chocolate agar

    BASAL LIQUID BASAL BASAL ENRICHED+ ENRICHED+ ENRICHED


    DIFFERENTIAL+ DIFFERENTIAL+
    INDICATOR INDICATOR
    AUTOCLAE AUTOCLAVE AUTOCLAVE AUTOCLAVE AUTOCLAVE , AUTOCLAVE
    TYNDALLIZATION

    PEPTONE+WATER PEPTONE+WATER+ Nutrient Nutrient Na- Nutrient agar+


    +NACL NACL+ LEAN MEAT Broth+ AGAR agar+10% TAUROCHOLATE(SELEC DEFIBRINATED
    AGAR DEFIBRINATED TIVE, SUPPRESS THE AND
    AND GROWTH OF GRAM + ANTICOAGULATE
    ANTICOAGULATED BACT.)+ D BLOOD
    BLOOD LACTOSE+NEUTRAL
    RED (
    DIFFERENTIATION
    BETWEEN LACTOSE
    FERMANTING OR NOT)
    BACTERIAL BACTERIAL OTHER MEDIA TO TEST GROWTH OF GRAM - Haemophilus,
    MOTILITY,INDOLE MOTILITY, OTHER PREPARATION HEMOLYTIC BACTERIA , Neisseria
    TEST, OTHER MEDIA + TO TEST PORPERTIES AND DIFFERENTIATION
    MEDIA PREPARATION PIGMENT GROWTH OF BETWEEN LF AND NLF
    PREPARATION PRODUCTION SOME BACTERIA
    7.4-7.5 7.4-7.5 7.5 7.5
    NUTRIENT AGAR NUTRIENT AGAR
    STERILIZED BY STERILIZED BY
    AUTOCLAVING AUTOCLAVING
    AND THEN COOL AND THEN COOL
    DOWN TO 50 DOWN TO 75-80
    DEGREE C DEGREE C
    THEN ADD THEN ADD
    ASEPTICALLY ASEPTICALLY
    HUMAN / ANIMAL HUMAN /
    STARILE BLOOD ANIMAL STARILE
    BLOOD
    Loeffler's Serum Dorsett's egg Robertson's cooked meat
    Slope medium Lowenstein - Jensen's media media

    ENRICHED ENRICHED ENRICHED+ SELECTIVE+DIFFERENTIAL ANAEROBIC+DIFFERENTIAL

    INSPISSATION INSPISSATION INSPISSATION AUTOCLAVE

    NUTRIENT BROTH NUTRIENT EGG( MAKES THE MEDIA ENRICHED)+ NUTRIENT BROTH +
    + BROTH + MALACHITE GREEN( SELECTIVE BY OX/SHEEP/HEART MUSCLE
    OX/SHEEP/HORSE EGGS(HEN'S) SUPPRESS THE GROWTH OF ALL BACT. + 0.5% GLUCOSE
    SERUM + EXCEPT MICOBACTERIA) + GLYCEROL(
    GLUCOSE GROWTH, DIFFERENTIATION
    BETWEEN HUMAN AND BOVINE TYPE
    OF MICROBACTERIUM) + MINERAL
    SALT SOLUTION

    GROWTH OF MAINTAINS GROWTH AND DRUG SENSITIVITY OF GROWTH OF ANAEROBIC


    DIPTHERIA AND LABORETORY M. TB BACTERIA
    PROTEOLYTIC STAINS OF M.
    ACTIVITY OF TB
    CLOSTRIDIUM
    PARASITOLOGY DRAWING
    BACTERIA GRAM MOTILITY COAGULASE CONFIRMATORY INDOLE SUGAR UREASE MEDIA NATURE
    TESTS
    Staphylococcus +ve coccus Non motile Coagulase +ve Tube coagulase +ve Catalase Oxidase -ve Gelatinase,Phosphatase, Nutrient Colony are circular,
    aureus are arranged +ve Dnase +ve agar convex,opaque,golden yellow
    in cluster pigment
    Bacillus subtilis +ve spore Motile Coagulase not Capsular staining reaction Nutrient Large circular,rough
    bearing done ,no medusa hair agar pigmented margin irregular
    bacilli, short appearance on nutrient greyish colour
    chain , agar and no inverted far
    spores are tree appearance on gelatin
    central or media
    subterminal
    Pseudomonus -ve non motile Catalase,oxidase,nitrate Indole Glucose +(acid) Urease -ve Nutrient Smooth shiney translucent
    aeruginosa sporing test , citrate test H2S +ve ring -ve Lactose and agar Moist
    bacilli mannitol -ve Sweet grape like
    haphazardly V.P test, MR test -ve

    Escherichia coli Gram -ve motile Catalase ,nitrate test , V.P Indole Glucose and urease -ve MacConkey Consflucent and discrete
    Straight rods test, MR test +ve +ve lactose acid + gas agar Smooth shiny non mucoid
    haphazardly convex pink lactose
    fermenting
    Oxidase, citrate test H2S -
    ve
    Klebsiella Gram -ve Non motile Catalase ,nitrate test , V.P Indole - Glucose and Urease +ve MacConkey Consflucent and discrete
    pneumoniae Straight rods test, citrate test +ve ve mannitol ,lactose agar circular
    haphazardly acid + gas mucoid dome shaped pink
    Oxidase MR test H2S -ve lactose fermenting
    Salmonella typhi Gram -ve Non motile Catalase ,nitrate test MR Indole - Glucose and Urease -ve MacConkey Colour less and non lactose
    Straight rods test H2S +ve ve mannitol acid agar fermenting
    haphazardly
    Oxidase, citrate test, V.P Lactose -ve
    test-ve
    Proteus vulgaris Gram -ve motile Catalase, nitrate, H2S Indole Glucose acid + Urease +ve MacConkey Consflucent and few discrete
    Straight rods KCN sensitivity +ve +ve gas agar Circular small NLF
    haphazardly low convex
    Oxidase, PPA, ODC -ve mannitol , Smooth moist pale
    Lactose -ve
    Proteus mirabilis Gram -ve motile Catalase, nitrate, H2S INDOLE Glucose acid + Urease +ve MacConkey Consflucent and few discrete
    Straight rods KCN sensitivityODC +ve -ve gas agar Circular small NLF
    haphazardly low convex
    Oxidase, PPA -ve mannitol , Smooth moist pale
    Lactose -ve

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