Anxious Attachment Style Predicts2015
Anxious Attachment Style Predicts2015
Nina Smyth, Lisa Thorn, Andrea Oskis, Frank Hucklebridge, Phil Evans &
Angela Clow
To cite this article: Nina Smyth, Lisa Thorn, Andrea Oskis, Frank Hucklebridge, Phil Evans &
Angela Clow (2015) Anxious attachment style predicts an enhanced cortisol response to group
psychosocial stress, Stress, 18:2, 143-148, DOI: 10.3109/10253890.2015.1021676
Article views: 76
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ISSN: 1025-3890 (print), 1607-8888 (electronic)
Abstract Keywords
Insecure attachment style is associated with poor health outcomes. A proposed pathway Group stressor, HPA axis, healthy females,
implicates the hypothalamic-pituitary-adrenal axis (HPA-axis), dysregulation of which is stress reactivity, saliva, trier social stress
associated with a wide range of mental and physical ill-health. However, data on stress test
reactivity in relation to attachment style is contradictory. This relationship was examined using
the novel Trier Social Stress Test for groups (TSST-G): a group-based acute psychosocial stressor. History
Each participant, in the presence of other group members, individually performed public
speaking and mental arithmetic tasks. Seventy-eight healthy young females (20.2 ± 3.2 years), Received 14 November 2014
in groups of up to six participants completed demographic information and the Vulnerable Revised 26 January 2015
Attachment Style Questionnaire (VASQ), and were then exposed to the TSST-G. Physiological Accepted 16 February 2015
stress reactivity was assessed using salivary cortisol concentrations, measured on seven Published online 11 March 2015
occasions at 10-min intervals. Vulnerable attachment predicted greater cortisol reactivity
independent of age, smoking status, menstrual phase and body mass index. Supplementary
analysis indicated that insecure anxious attachment style (high scores on the insecurity and
proximity-seeking sub-scales of the VASQ) showed greater cortisol reactivity than participants
with secure attachment style. Avoidant attachment style (high scores for insecurity and low
scores for proximity seeking) was not significantly different from the secure attachment style.
Attachment style was not associated with the timing of the cortisol peak or post-stress recovery
in cortisol concentrations. These findings in healthy young females indicate subtle underlying
changes in HPA axis function in relation to attachment style and may be important for future
mental health and well-being.
Introduction et al., 2013; Jinyao et al., 2012; Puig et al., 2013). The
biologic underpinnings of these links however are not clear.
Attachment style is suggested to be important for regulating
One of the proposed pathways implicates the hypothal-
threat appraisal, stress response and recovery from stress,
amic-pituitary-adrenal (HPA) axis (Repetti et al., 2002),
although the mechanisms underlying this complex interplay
dysregulation of which is associated with a wide range of
are not well-understood (Diamond, 2001). Within the adult
mental and physical ill-health (McEwen, 2000). A flattened
attachment literature, insecure attachment style is generally
diurnal rhythm of cortisol secretion has been reported in
conceptualized along two dimensions, namely attachment
anxious attachment style (Oskis et al., 2011; Quirin et al.,
anxiety and avoidance (Brennan et al., 1998). A securely
2008); however, there are mixed findings from studies
attached individual is considered to be an individual with low
examining reactivity of the HPA axis in relation to attachment
levels of both (Brennan et al., 1998). High attachment
style. For example, in one study, avoidant (but not anxious)
anxiety is associated with preoccupation with the availability
attachment predicted enhanced stress-induced cortisol
and responsiveness of the other, maximization of negative
responding in females (Powers et al., 2006) while the opposite
experiences and hyper-vigilance to potential threat.
was found in another study (Quirin et al., 2008). Other studies
Attachment avoidance is associated with a tendency to
have reported insecure dismissing attachment style to predict
devalue intimacy and dependency and maximize autonomous
enhanced cortisol reactivity (Pierrehumbert et al., 2012;
behavior strategies when faced with potential threat. Insecure
Rifkin-Graboi, 2008) whereas, secure and dismissive attach-
attachment style is known to predict a range of poor physical
ment styles have been reported as similar elsewhere
and mental health outcomes (Bifulco et al., 2002a,b; Carr
(Kidd et al., 2011). Further studies show no relationship
between cortisol responding to a stressor and attachment style
Correspondence: Dr Angela Clow, Psychophysiology and Stress
(Ditzen et al., 2008; Smeets, 2010).
Research Group, Department of Psychology, University of
Westminster, 115 New Cavendish Street, London W1W 6UW, UK. Whether attachment style predicts acute stress responding
Tel: +44-0207-911-5000. E-mail: clowa@wmin.ac.uk remains unclear. The disparity in the literature may in part be
144 N. Smyth et al. Stress, 2015; 18(2): 143–148
related to the wide array of methodologies that have been used purposes, and another participant did not complete the
to investigate this issue. Stressors have ranged from the Trier attachment questionnaire. A single participant was removed
Social Stress test (TSST) for individuals (Ditzen et al., 2008; from the data set on the basis that their cortisol data were >5
Smeets, 2010) to experimental conflict negotiation (Powers standard deviations (SD) above the mean for each sample, and
et al., 2006), visualization of hypothetical distressing situ- their data remained as outliers following square root trans-
ations (Rifkin-Graboi, 2008) and behavioral interference tasks formation. Analyses were performed on 78 participants, age
(Kidd et al., 2011). The most commonly used tool to assess ranging from 18 to 33 (mean ± SD: 20.1 ± 3.1) years.
attachment style in adult stress reactivity studies is the Participants were ethnically diverse; of those who disclosed
Experiences in Close Relationships Scale (Brennan & Shaver, their ethnicity, 26 were Asian (Indian, Chinese, Pakistani,
1995), which assesses attachment in romantic relationships. Bangladeshi and Arabic), 31 were white European, 13 were
The Vulnerable Attachment Style Questionnaire (VASQ; African Caribbean and 4 were mixed race.
Bifulco et al., 2003) is arguably a more appropriate measure To control for gender differences in cortisol reactivity, only
for use in research investigating attachment and HPA axis females were recruited. To reduce the impact of variables
activity as, rather than romantic attachment, it focuses on how known to influence cortisol reactivity, exclusion criteria
individuals generally relate to others. Furthermore, it per- included medication, illness and history of psychiatric illness.
forms somewhat better in predicting depression than other As cortisol reactivity is influenced by the menstrual cycle and
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self-report measures of attachment (Bifulco et al., 2003), as body mass index (BMI) the number of days since last period
well as predicting negative psychosocial well-being and was recorded, as was height and weight (Dockray et al., 2009;
mental health in university students (Carr et al., 2013). The Smyth et al., 2013). Two participants used oral contracep-
VASQ was developed and validated in relation to an in-depth tives. The majority of participants (86%) were non-smokers.
interview procedure (Attachment Style Interview; Bifulco
et al., 2002a,b), which has been used in previous research Vulnerable Attachment Style Questionnaire
examining HPA axis activity and attachment style (Oskis The VASQ (Bifulco et al., 2003) is a brief self-report tool,
et al., 2011, 2014). which assesses general adult attachment. It is designed to
The TSST (Kirschbaum et al., 1993) comprises uncon- assess overall attachment vulnerability as well as two
trollability and socio-evaluative threat known to reliably dimensions of attachment: a global dimension of attachment
activate HPA axis function (Dickerson & Kemeny, 2004) and insecurity common to all insecure subtypes (representing a
has recently been adapted for use in group settings: the TSST- deep-rooted mistrust of others and their motives) and a
G (von Dawans et al., 2011). A primary motivation was to proximity-seeking dimension reflecting the strategy individ-
increase the rate of participant exposure to the TSST but it uals use to manage their insecurity (i.e. some individuals with
provides the opportunity to examine the impact of social high insecurity develop excessive neediness and vigilance of
dynamics on stress reactivity (Häusser et al., 2012). In the others, while other individuals develop an aversion to
present, study we adapted the TSST-G to maximize closeness with others). The scale comprises 22 items
opportunities for group interaction which may attenuate or measured on a 5-point scale, ranging from strongly disagree
increase stress reactivity depending on the characteristics of to strongly agree. Low scores on the 12-item insecurity
the individual within the group. Given that, those with high subscale (e.g. ‘‘I find it hard to trust others’’ and ‘‘People let
attachment insecurity easily perceive threats in their environ- me down a lot’’) represent secure attachment and high scores
ment, frequently experience social interactions as stressful reflect insecure attachment. The proximity-seeking subscale
and excessively ruminate about psychologically distressing consists of 10 items (e.g. ‘‘I get anxious when people close to
experiences (Burnette et al., 2009; Shaver & Mikulincer, me are away’’ and ‘‘I look forward to spending time on my
2002) they might find the group version of the TSST own’’). Low scores represent propensity for avoidant behavior
particularly stressful. We chose to investigate an all-female and high scores reflect a need for closeness with others.
sample since gender is known to moderate the link between Cronbach’s a was 0.81 for the insecurity scale and 0.74 for the
attachment style and HPA reactivity (Kiecolt-Glaser et al., proximity scale. A total attachment vulnerability measure can
1996; Kirschbaum et al., 1995; Stroud et al., 2002). Young, be derived by summing items on both scales. The VASQ can
healthy participants were recruited to explore whether also be used to categorize participants according to secure,
attachment style might be a pre-clinical indicator of vulner- insecure anxious or insecure avoidant attachment styles. The
ability rather than a consequence of concurrent poor health. insecure anxious attachment style category is derived
Due to discrepancies in the stress reactivity and attachment from high scores on both insecure and proximity-seeking
literature, the aim of this study was to examine self-reported measures. The insecure avoidant type category is derived
attachment and physiological stress responding to a group from high scores on the insecurity scale and low scores on the
psychosocial stressor. proximity scale.
Methods
Procedure
Participants
The study was approved by the University of Westminster
Eighty-one female undergraduate student participants were Ethics Committee. Following recruitment, groups of partici-
recruited. They did not receive financial incentives but did pants were invited to attend a test session at a set time and
receive course credits. Cortisol data was missing for one place. In line with best practice, guidelines (Kirschbaum
participant as the salivary volume was insufficient for assay et al., 1993; Smyth et al., 2013) testing commenced in the
DOI: 10.3109/10253890.2015.1021676 Stress reactivity and attachment style 145
afternoon between 13:00 and 15:00 h, to control for changes and S7, at 62 min). This cortisol profile allowed us to capture
in basal cortisol secretion in the morning and following the the rise in cortisol, the cortisol peak and the decline of cortisol
post-prandial period. Participants were asked to refrain from (i.e. the recovery period) (Dickerson & Kemeny, 2004; Smyth
food, caffeine, alcohol, exercise and smoking 30 min prior to et al., 2013). Saliva samples were frozen at 20 C until
the research session. The TSST-G (von Dawans et al., 2011) assayed at the University of Westminster. Samples were
included three main phases: the group preparatory period thawed and centrifuged for 10 min at 3500 rpm. Cortisol
(30 min); the group stress task period (22 min); and a group concentrations were determined by enzyme-linked immuno-
resting and debriefing period (40 min). During the preparatory sorbent assay developed by Salimetrics LLC (State College,
period, groups of up to six participants met in Room 1 where PA). The standard range in the assay was 0.33–82.77 nmol/l.
they were informally seated around a single table and Intra- and inter-assay variations were both <10%.
introduced to the experimenter, they were free to talk to
each other at this time. Statistical analysis
Following informed written consent, participants com- Cortisol data were moderately skewed and, therefore, a square
pleted in silence demographic questions, the date of their last root transformation was applied which normalized distribu-
menstruation and the VASQ, if they had not already tions, although cortisol concentrations shown in figures and
completed it on-line (it had been available since the beginning tables are representative of original units. Descriptive statis-
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of the recruitment period). Each participant then received a tics were explored for each cortisol sample measured
large sticker with a number between 1 and 6. They were throughout the TSST-G procedure, and a one-way within-
informed that they would be identified with this number subjects analysis of variance (ANOVA) was conducted to
during the task period and that the numbers would be called in examine differences in cortisol over time. Within-subjects
a random order. Participants were then introduced to the contrasts were used to assess the pattern of cortisol secretion.
saliva-sampling method. Following this, participants were As participants performed the TSST-G tasks at slightly
given 10-min quiet time to prepare notes for a mock job different time-points, cortisol reactivity was computed for
interview. They were asked to prepare a free 2-min speech as each individual as their peak sample minus baseline. Cortisol
if applying for a job of their choice and to introduce recovery was computed as individual peak sample minus
themselves to the committee. They were asked to convince the sample 7 (recovery). Pearson’s correlation coefficients were
committee that they were the most suitable candidates for the used to examine relationships between these cortisol indices,
position. After this preparatory period, the baseline saliva VASQ attachment measures and demographics variables.
sample was collected immediately prior to leaving Room 1. Significant relationships between cortisol and attachment
Participants were taken into Room 2 (a short distance away) measures were examined in a multiple regression analysis
and instructed to stand in a straight line in front of the already controlling for variables known to affect cortisol stress
seated committee, comprising one woman and one man. The reactivity. Participants were categorized according to the
committee were wearing white laboratory coats and there VASQ attachment style: secure (n ¼ 20), insecure anxious
were two conspicuous video cameras pointing at the partici- (n ¼ 37) and insecure avoidant (n ¼ 21). A one-way between-
pants. A committee member called the number of each subjects ANOVA explored group differences in cortisol stress
participant in turn in a random order to make a 2-min speech reactivity and Bonferroni post hoc tests were applied. 2
as if applying for a job. After all participants gave their speech square test was used to examine the association between
(a total of up to 12 min), the committee asked the participants, participants’ peak cortisol time and attachment style group.
in the same order, to serially subtract the number 17 from a
given number (e.g. 4878) as fast and accurately as possible for
Results
80 s. Each participant received an individual starting number
to avoid learning effects. Standard responses from the Results indicated that the TSST-G induced an overall cortisol
committee were followed where participants ended their response in this sample [F(6,462) ¼ 7.623, p < 0.001), illu-
speech before the 2-min duration (e.g. ‘‘you still have time, strated in Figure 1. Within-subjects contrasts revealed a
please continue’’) or failed in the subtraction task (e.g. ‘‘you significant quadratic effect [F(1,77) ¼ 23.807, p < 0.001], such
made a mistake please start again from the number. . .’’) (von that on average cortisol increased from baseline peaked at the
Dawans et al., 2011). Immediately after all participants had fourth sampling point (10 min after the completion of the
completed the TSST-G, participants were returned to Room 1, TSST-G) and subsequently declined.
where they collected saliva samples every 10 min up to 40 min Relationships between cortisol data and attachment vari-
following the TSST-G period. During this time they were ables measured by the VASQ were examined using focused
debriefed. composite cortisol indices: individual peak sample minus
baseline (cortisol reactivity) and individual peak sample
minus sample 7 (recovery). Descriptive statistic and intercor-
Saliva-sampling collection
relations for all variables are presented in Table 1.
Cortisol was measured in saliva samples collected using There was a significant positive relationship between
Salivettes (saliva-sampling devices; Sarstedt Ltd., Leicester, cortisol reactivity and VASQ vulnerability score (r ¼ 0.289,
UK) at baseline (immediately before the TSST-G: S1, at 0 min) p ¼ 0.010) in that participants with a higher level of
immediately after the public speaking task (S2, at 12 min), vulnerable attachment exhibited a greater increase in corti-
after the mental arithmetic task (S3, at 22 min), and every sol from baseline to peak value. In other terms, participants
10 min up to 62 min (S4, at 32 min S5, at 42 min, S6, at 52 min who demonstrated an insecure anxious attachment style
146 N. Smyth et al. Stress, 2015; 18(2): 143–148
(those scoring highly on both VASQ subscales) displayed known to affect cortisol reactivity were included in the model
greater cortisol reactivity. With regards to the dimensions of (Table 2). Vulnerable attachment and age remained signifi-
the VASQ, insecurity was significantly positively correlated cant independent predictors of cortisol reactivity.
with cortisol reactivity, whereas proximity was not. There The VASQ can be used to group participants according to
were no relationships between attachment measures and secure, anxious or avoidant attachment styles. The insecure
cortisol recovery. There were also no relationships between anxious attachment style category is derived from high scores
cortisol measures and pertinent demographic characteristics, on both insecure and proximity-seeking measures. In a
apart from age, which was positively related with both supplementary analysis, a one-way between subjects
cortisol reactivity and recovery. In terms of VASQ attachment ANOVA was performed to examine the difference in cortisol
measures, vulnerability was unrelated to demographic vari- reactivity between the three groups. There was a significant
ables; however, insecurity was positively related with age, and effect of attachment style group on cortisol reactivity
proximity was positively correlated with smoking status. [F(2,5) ¼ 5.300, p ¼ 0.007] (Figure 2). Bonferroni post hoc
A multiple regression analysis was conducted to examine tests indicated that the insecure anxious group was signifi-
whether the relationship between cortisol reactivity and cantly different from the secure group (p ¼ 0.011). There was
vulnerable attachment remained significant when variables no association between when participants peaked and their
attachment style group (2 ¼ 16.405, p ¼ 0.173).
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Discussion
Vulnerable attachment, determined by the VASQ, predicted
greater cortisol reactivity to a group psychosocial stressor
independent of age, smoking status, menstrual phase and
BMI. While there were no differences in the timing of the
cortisol peak, supplementary analysis revealed that partici-
pants with an insecure anxious attachment style (a combin-
ation of high scores on the insecurity and proximity-seeking
sub-scales of the VASQ) showed greater stress-induced
Predictors b t p Value
VASQ vulnerability 0.241 2.205 0.031
Age 0.322 2.902 0.005
Menstrual cycle phase 0.118 1.132 0.261
Figure 1. Mean (±SEM) salivary free cortisol concentrations (nano- Smoking status 0.182 1.706 0.092
moles per litre) for all participants (n ¼ 78). (A) Immediately before BMI 0.051 0.468 0.641
onset of the TSST-G; (B) mid-way through the TSST-G and (C) R2 0.162 (p ¼ 0.003)
immediately after the end of the TSST-G.
Table 1. Descriptive statistics and intercorrelations between cortisol, VASQ attachment measures and demographic data (n ¼ 78).
Correlations
Cortisol Menstrual Smoking
Variables Descriptives recovery Vulnerability Insecurity Proximity Age cycle phase status BMI
Cortisol reactivity 4.47 (6.50) 0.185 0.289* 0.269* 0.177 0.349** 0.141 0.103 0.011
nmol/l M (SD)
Cortisol recovery 4.20 (4.50) 0.020 0.047 0.016 0.276* 0.176 0.015 0.017
M (SD)
VASQ Vulnerability 60.98 (9.81) 0.775** 0.770** 0.209 0.037 0.163 0.119
nmol/l M (SD)
VASQ Insecurity 33.37 (6.39) 0.193 0.247* 0.051 0.001 0.035
M (SD)
VASQ Proximity 27.61 (6.32) 0.075 0.006 0.254* 0.150
M (SD)
Age (years) 20.22 (3.21) 0.054 0.099 0.218
M (SD)
Menstrual cycle phase % 38.5 0.017 0.007
(luteal)
Smoking status 85.9 0.111
% (non-smoker)
BMI 21.46 (3.76)
M (SD)
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Psychoneuroendocrinology 36(6):771–9.
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