Scribd Logo
Upload

Welcome to Scribd!

  • 10 views

    Industrial Microbiology Session 8

    Uploaded by

    Matthew Ghaffari
    This document discusses media formulation for growing microorganisms in industrial settings. It explains that microbes need specifically designed media with controlled conditions for growth. The medium must contain all required nutrients for the microbe, including sources of carbon, nitrogen, phosphorus and other minerals. The composition depends on the microbial species and its natural habitat. The document then outlines the key chemical requirements for microbial growth, including carbon, nitrogen, sulfur, phosphorus and other essential elements and trace elements.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Industrial Microbiology Session 8

    Uploaded by

    Matthew Ghaffari
    10 views9 pages
    This document discusses media formulation for growing microorganisms in industrial settings. It explains that microbes need specifically designed media with controlled conditions for growth. The medium must contain all required nutrients for the microbe, including sources of carbon, nitrogen, phosphorus and other minerals. The composition depends on the microbial species and its natural habitat. The document then outlines the key chemical requirements for microbial growth, including carbon, nitrogen, sulfur, phosphorus and other essential elements and trace elements.

    Original Description:

    Industrial Microbiology Session 8

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document discusses media formulation for growing microorganisms in industrial settings. It explains that microbes need specifically designed media with controlled conditions for growth. The medium must contain all required nutrients for the microbe, including sources of carbon, nitrogen, phosphorus and other minerals. The composition depends on the microbial species and its natural habitat. The document then outlines the key chemical requirements for microbial growth, including carbon, nitrogen, sulfur, phosphorus and other essential elements and trace elements.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    10 views9 pages

    Industrial Microbiology Session 8

    Uploaded by

    Matthew Ghaffari
    This document discusses media formulation for growing microorganisms in industrial settings. It explains that microbes need specifically designed media with controlled conditions for growth. The medium must contain all required nutrients for the microbe, including sources of carbon, nitrogen, phosphorus and other minerals. The composition depends on the microbial species and its natural habitat. The document then outlines the key chemical requirements for microbial growth, including carbon, nitrogen, sulfur, phosphorus and other essential elements and trace elements.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 9

    Industrial Microbiology

    Instructor: Bita Zamiri


    Quick Recap
    Microbial Growth and Nutrition
    The osmotic concentration of a habitat has such profound effect pH dramatically affects microbial growth. Each species has a definite
    on microorganisms pH growth range and pH growth optimum.

    Water activity is inversely related to osmotic pressure

    Most organisms spend their lives on land or on the surface of water


    always subjected to a pressure of 1 atm and are never affected
    significantly by pressure

    2
    Today we will study:

    • Chemical requirements for growth and media formulation


    Media Formulation
    • The first task for any industrial microbiologist is to find a suitable microorganisms for use in the desired process.

    • A wide variety of alternative approaches are available, ranging from isolating microorganisms from the environment to
    using sophisticated molecular techniques to modify an existing microorganism.

    • Only a minor portion of the microbial species in most environments have been isolated and cultured.

    • \.
    Media Formulation

    • \.
    Media Formulation

    • For many industrial processes, microorganisms must be grown using specifically designed media under carefully
    controlled conditions, including temperature, aeration and nutrient feeding during the course of fermentation.

    • The growth of microorganisms under such controlled environments is expensive and this approach is used only when
    the desired product can be sold for a profit.

    • The high costs arise from the expense of development of a particular microorganisms to be used in large-scale
    fermentation, the equipment, medium preparation, product purification and packaging along with marketing efforts.
    Media Formulation

    • The medium used to grow a microorganisms (medium formulation or development) is critical because it can
    influence the economic competitiveness of a particular process.

    • Frequently, lower cost cruse materials are used as sources of carbon, nitrogen and phosphorous.
    Media Formulation

    • The ability to grow and maintain microorganisms is only possible if suitable culture media is available.

    Culture Media: a solid or liquid preparation used to grow, transport and store microorganisms.

    • To be effective, the medium must contain all the nutrients the microorganisms requires for growth.

    • Although all microorganisms need sources of energy, carbon, nitrogen, phosphorus, sulfur and various minerals,
    the precise composition of a satisfactory medium will depend on the species one is trying to cultivate because
    nutritional requirements vary.

    • Knowledge of a microorganisms normal habitat often is useful is selecting an appropriate culture medium because its
    nutrient requirements reflect its natural surrounding.

    Elemental composition of bacteria


    (% dry weight)
    Media Formulation

    Chemical Requirements for Growth


    Carbon:
    • Makes up 50% of the dry weight of cells.
    • It is a key elements in the structural backbone of all organic compounds.
    • Chemoheterotrophs obtain carbon from organic molecules and autotrophs obtains carbon from CO2

    Nitrogen:
    • Makes up 14%of the dry weight of cells.
    • It is used to synthesize amino acids, DNA and RNA.

    Sulfur:
    • Used to form proteins and some vitamins (thiamin and biotin)

    Phosphorus:
    • Used to forms DNA, RNA, ATP and phospholipids

    Other elements:
    • Potassium, magnesium and calcium are often required as enzyme cofactors. Calcium is required for cell wall synthesis
    in Gram positive bacteria.

    Trace elements
    • Many are used as enzyme cofactors (Iron, Copper, Molybdenum, Zinc)

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy