Genetics and Molecular Biology Division Institute of Biological Sciences, College of Arts and Sciences University of The Philippines Los Baños

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GENETICS AND MOLECULAR BIOLOGY DIVISION

Institute of Biological Sciences, College of Arts and Sciences University of the Philippines Los Baños

Name: __________________________________ Date Submitted: __________________


Lab Section: Laboratory Instructor: _________________

Exercise 7
Quantitative Inheritance
ACTIVITY SHEET

DIRECTION: Show all computations for the following problems.

A.
The phenotypic data below are the shoot lengths of 25 F1 and 25 F2 rice plants at seedling

stage, produced from the cross of IR29 and Hasawi rice varieties. The mean lengths of the shoot
from IR29 and Hasawi are 23.1 cm and 46.7 cm, respectively. Tabulate and plot the frequency
distributions of the F1 and F2 generations. Use the formulas below in the computations. From
each distribution calculate the mean, the variance, and the standard error of the mean. Round

off answers to two decimal places. What is the main difference between F1 and F2 distributions?

Formulas needed:
Range (R) = maximum – minimum (Use the same no. of decimal places as original data.)

No. of phenotypic classes (K) = 1 + 3.3logn (Round up answer to an integer. Number may
still be increased or decreased as needed.)
n = total no. of values in sample data

Class interval (CI) = R/K


Use final answers for R and K in computation. For the final answer, use the same no. of
decimal places as original data. The CI represents the difference between the lower limits of
two consecutive phenotypic classes.

Shoot Length of F1 (cm) Shoot Length of F2 (cm)


37.9 34.3 35.4 37.4 34.9 29.7 48.2 39 26.7 38.1
35.6 33.7 34.8 34.9 36.7 36.1 51 35.6 33.4 34.8
33.7 32.4 36 33.8 40.5 43.8 34.8 42.2 30.7 41.2
34 31.8 35.7 28.4 35 49.8 44.8 42.8 48.7 40.7
35.2 35 35.6 34.3 34.1 50.1 40.6 38.2 26 34.8

*For Problems B and C, consider these reminders in the use of the following formulas:

VP=VG +VE +VGxE: For VGxE, do not multiply VG and VE, that is not how the value is calculated. In
this exercise, no values will be used for VGXE or is assumed to be zero (0).

VG = VA+VD+ VI: Variance due to epistatic effect is difficult to measure and usually is not
considered in the equation. If VI is not given in the problem, the assumption is it has a value

This course material is intended solely for the personal use of the student enrolled in BIO 30. No part of this course material can be reproduced, reposted, or replicated in any form without
permission from the Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UPLB
Exercise 7: Quantitative Inheritance BIO 30. Genetics
of zero (0).

This course material is intended solely for the personal use of the student enrolled in BIO 30. No part of this course material can be reproduced,
reposted, or replicated in any form without permission from the Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UPLB
Exercise 7: Quantitative Inheritance BIO 30. Genetics
B.
The mean and standard deviation of plant height from two rice plants (P 1 and P2) and their

progeny (F1 and F2) and a backcross generation (P1 x F1) are shown below. Complete the table by
calculating the variances and coefficient of variation (round off to two decimal places) for each
population and answer the questions which follow.

Population Mean (in) Standard Variance Coefficient of


Deviation Variation (CV)

P1 34.2 1.2
P2 65.3 0.8
F1 44.2 1.3
F2 46.3 1.9
BC1 39.7 1.1

1. Explain the possible reasons for the observed differences in the sample means. Account for
the differences in the sample means of P 1 and P2. Similarly, account for the differences in the
sample means of the F 1 and F2. Compare the difference in the parental generations with that
in the filial generations.
2. Interpret the CV values from each population.
3. Compare the sample variances of P1 and P2. Account for any differences. Similarly, compare
the sample variances of the F1 and F2 generations, and account for any differences. Give the
possible causes of variation in each generation.
4. Calculate the broad-sense heritability of plant height in this species. Show all solutions. Interpret your
results.

This course material is intended solely for the personal use of the student enrolled in BIO 30. No part of this course material can be reproduced,
reposted, or replicated in any form without permission from the Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UPLB
Exercise 7: Quantitative Inheritance BIO 30. Genetics
C.
In a horse population, three different traits showing continuous distribution were measured,
and their variances are shown in the table below:

Traits
Cutting Racing Jumping
Ability Speed Ability
Variance
Phenotypic 96.0 39.2 39
Environmental 42.0 27.8 16.8
Additive 39 12.4 12.0
Dominance 10 3.1 9

1. Calculate the broad sense and narrow sense heritabilities for each trait. Show solutions.
2. In the population of animals studied, which trait would respond best to selection for breeding
purposes? Explain your answer.

This course material is intended solely for the personal use of the student enrolled in BIO 30. No part of this course material can be reproduced,
reposted, or replicated in any form without permission from the Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UPLB

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