Application of Recombinant DNA

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Transgenic Organisms

• These are organisms altered by genetic engineering. The


genetic material can be changed by other than random
natural breeding or by gene transfer which is the moving of a
gene from one organism to another.

Genetically Modified Organisms


• Genetically modified organisms (GMOs) are living organisms
whose genetic material has been artificially manipulated in a
laboratory through genetic engineering. This creates
combinations of plant, animal, bacteria, and virus genes that
do not occur in nature or through traditional crossbreeding
methods.

Applications of Recombinant DNA

1. Medicine Application

• Genetic engineering has been used to mass-produce insulin,


human growth hormones, follistim (for treating infertility),
human albumin, monoclonal antibodies, antihemophilic
factors, vaccines, and many other drugs.

2. Industrial Application
• Genetically designed bacteria are put into use for generating
industrial chemicals. A variety of organic chemicals can be
synthesized at large scale with the help of genetically
engineered microorganisms. Glucose can be synthesized
from sucrose with the help of enzymes obtained from
genetically modified organisms.
• Bacteria that metabolize petroleum and other toxic materials
have been developed.
• Scorpion toxin gene has been inserted into autographa
California multicapsid nuclear polyhedrosis virus (AcMNPV)
which kills cabbage looper and reduce crop damages.
• Development of new strains for additional bioprocesses
3. Agricultural Application
• An important application of recombinant DNA technology is
to alter the genotype of crop plants to make them more
productive, nutritious, rich in proteins, disease resistant, and
less fertilizer consuming. Recombinant DNA technology and
tissue culture techniques can produce high yielding cereals,
pulses and vegetable crops.
Scientists have developed transgenic potato, tobacco, cotton,
corn, strawberry, grape seeds that are resistant to insect pests
and certain weedicides.
• Genes of interest is inserted into plant with Ti plasmid
obtained from Agrobacterium tumefaciens.
• Pseudomonas syringae that protects plants from frost
damage is used against plant frost damage because they
lack the protein that induce the formation of ice-crystals.
• Insertion of Bacillus thuringiensis that produces a protein
which is toxic to insects but not to humans.

4. Energy Production
• Recombinant DNA technology has tremendous scope in
energy production. Through this technology Li is now
possible to bioengineer energy crops or biofuels that grow
rapidly to yield huge biomass that used as fuel or can be
processed into oils, alcohols, diesels, or other energy
products.

• The waste from these can be converted into methane.


Genetic engineers are trying to transfer gene for cellulase to
proper organisms which can be used to convert wastes like
sawdust and cornstalks first to sugar and then to alcohol.

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