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Chromatography Lab Report

This document summarizes a thin layer chromatography (TLC) lab experiment. Various compounds including aspirin, acetaminophen, ibuprofen, caffeine, and an unknown sample were tested. The Rf values of the known compounds were calculated. The unknown sample was found to have components that matched acetaminophen, ibuprofen, and caffeine, indicating it contained those three compounds. A mobile phase of ethyl acetate and hexane provided better separation than ethyl acetate alone. Potential sources of error include contamination of the TLC paper.

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41 views

Chromatography Lab Report

This document summarizes a thin layer chromatography (TLC) lab experiment. Various compounds including aspirin, acetaminophen, ibuprofen, caffeine, and an unknown sample were tested. The Rf values of the known compounds were calculated. The unknown sample was found to have components that matched acetaminophen, ibuprofen, and caffeine, indicating it contained those three compounds. A mobile phase of ethyl acetate and hexane provided better separation than ethyl acetate alone. Potential sources of error include contamination of the TLC paper.

Uploaded by

Senia Bu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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CHEM 2400 ORGANIC CHEMISTRY 1 LABORATORY

Thin Layer Chromatography Lab Report

1. Table of Reagents and product(s):


Name Density MW(g/mol) BP MP(°C) Formula Structure
(g/ml) (°C)
Hexane 0.66 86.18 68.7ºC -95ºC C6H14

Water 1.0 18.02 100ºC 0ºC H2O

Acetone 0.79 58.08 56ºC -95ºC C3H6O

Ethylacetate 0.90 88.11 77.1ºC -84ºC C4H8O2

Aspirin 1.35 180.16 140ºC 135ºC C9H8O4

Acetaminophen 1.26 151.16 420ºC 169ºC C8H9NO2

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Ibuprofen 1.03 206.29 157ºC 76ºC C13H18O2

Caffeine 1.23 194.19 178ºC 236ºC C8H10N4O2

2. Data and calculations:


5𝑐𝑚
Rf of aspirin: 6𝑐𝑚 = 0.83

2.2𝑐𝑚
Rf of acetaminophen: = 0.37
6𝑐𝑚

5.5𝑐𝑚
Rf of ibuprofen: = 0.92
6𝑐𝑚

0.6𝑐𝑚
Rf of caffeine: = 0.1
6𝑐𝑚

Rf of unknown:
0.5𝑐𝑚
• Dot lined up with caffeine: = 0.083
6𝑐𝑚
2𝑐𝑚
• Dot lined up with acetaminophen: 6𝑐𝑚 = 0.33
5.5𝑐𝑚
• Dot lined up with ibuprofen: = 0.92
6𝑐𝑚

3. Conclusion:

In this experiment, two separate pieces of thin layer chromatography (TLC) paper were cut and singular

drops of 5 different substances were placed on each of them: aspirin, acetaminophen, ibuprofen, caffeine,

and an unknown substance. The purpose of the experiment was to identify what the unknown substance was
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comprised of using a UV radiation apparatus. The Rf values of aspirin, acetaminophen, ibuprofen, and

caffeine were found to be 0.83, 0.37, 0.92, and 0.1, respectively. The unknown substance showed to have

three dots: one that aligned with acetaminophen, one with ibuprofen, and one with caffeine. The Rf values

for each of these dots were 0.083 (acetaminophen), 0.33 (ibuprofen), and 0.92 (caffeine). This indicates that

the unknown was comprised of three ingredients: acetaminophen, ibuprofen, and caffeine. The mobile phase

with ethyl acetate and hexane was better than the phase with pure ethyl acetate because ethyl acetate is a

polar substance while hexane is nonpolar. By having 6 mL of ethyl acetate and 4 mL of hexane, the solvent

could have the possibility of interacting with solutions of varying polarities to give the different Rf values. A

potential source of error that may have occurred during the experiment was contamination of the TLC paper.

Because the UV radiation apparatus depicts organic compounds on the paper, saliva could also appear under

the UV light, and thus show more dots on the paper than there should be. This can lead to a misinterpretation

of the ingredients’ Rf values and ultimately provide inaccurate results.

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