For students

Max marks : 04 Time allotted : 04 minutes

Task

1. IDENTIFY THE GIVEN MEDIUM : 01

2. WHICH DYE IS ADDED TO THIs MEDIUM : 01
3. WHAT IS THE USE OF THIS MEDIUM : 01
4. GROWTH OF THE SPECIFC ORGANISMS WILL BE OBSERVED AFTER HOW
MUCH TIME ON THIS MEDIUM : 01

For examiner

S.No Key Marks

1 Lowenstein Jensen medium 01
2 Malachite Green 01
3 Medium is used for the growth of 01
mycobacteria
4 03 – 04 weeks 01

1
For students
Max marks : 04 Time allotted : 04 minutes

Carefully examine the device / photograph and answer the following questions

Task

1. Give the findings of the device

2. Name two serological markers which are positive in acute Hepatitis B virus infections
3. Name the type of detection Method
4. Give other clinical applications of the technique

For examiner

S.No Key Marks

1 Device shows positive result for HBsAg 01
2 HBeAg. Anti HBc IgM 01
3 Immunochromatographic test 01
4 Hepatitis C virus Antibody detection, 01
Pregnancy test , Dengue virus antibodies

2
For students
Max marks : 04 Time allotted : 04 minutes

A Female 54 yrs old living in a small house

with her large family was admitted in hospital with a 5 month history of progressive weakness
and weight loss of 13 kgs. She also had fever and productive cough of yellowish sputum
streaked with blood. On physical examination there are crackles in the apex of right lung. X ray
chest shows a cavity in that area. ZN-Stained slide of sputum is provided to you. Carefully
examine the given slide and answer the following questions

Task

1. Which medium is selective for growth of tubercle bacilli?

2. How much time is required for tubercle bacilli to grow in the selective medium
3. Name the dermal test for tubercle bacilli showing hypersensitivity reaction ‘
4. Name the reagent used in dermal test for tubercle bacilli ?

For examiner

S.No Key Marks

1 Lowenstein Jensen’s Medium 01
2 4 – 7 Weeks 01
3 Tuberculin test 01
4 Purified Protein Derivative , PPD 01

3
For students
Max marks : 04 Time allotted : 04 minutes

You are shown photograph of a fungus recovered from ear swab of patient with otitis externa.
This preparation was made in Lactophenol cotton blue Carefully examine the given photograph
and answer the following

Task

5. Give name of the fugus based on the Microscopic appearance

6. Name three common species of this Fungi
7. Name two other diseases caused by this organism

For examiner

S.No Key Marks

1 Aspergillus 01
2 Fumigatus, A. flavus, A. niger 01
3 Allergic aspergillosis, Broncho 01
pulmonary aspergillosis , aspergilloma

4
For candidate

Marks : 04 Time allotted : 04 minutes

Task

1. Report the reactions observed in the provided TSI Medium

2. What is the likely organism that would give these reactions
3. Is this organisms motile or non motile

For examiners

Sr. No Key Max Marks

1 Alkaline slant, Acidic Butt, H2S 02
positive , Gas negative
2 Salmonella Typhi 01
3 Motile 01

5
For Candidate

Marks : 04 Time allotted : 04

Task :

1. Identify the organism

2. Identify the Acute clinical condition caused by the organism as shown in the above
picture
3. Describe the organism
4. What is the mode of transmission

For examiners

Sr. No Key Max Marks

1 Adults Ascaris lumbricoides 01
2 Intestinal Obstruction 01
3 Round worm with anterior and 01
posterior end,
4 Feco oral route 01

6
For Candidate

Marks : 04 Time allotted : 04

Task

1. Give the microscope finding and name the organism

2. Write TWO characteristic feature of of the egg seen
3. Give ONE complication that can occur in persons infected with this organism

For examiners

Sr. No Key Max Marks

1 Eggs of Hook worm 01
2 Non bile stained egg with 02
segmented ovum
3 Iron deficiency anemia 01

7
For Candidate

Total Marks : 04 Time allotted : 04 Marks

A 12 year old girl was bitten

by a dog on the face. Dog was
unusually aggressive and
irritable. Dog was killed and
brain autopsy was done.
Histopathology picture is shown
above
‘Diseased Dog Dog’s Brain Autopsy picture

1. What is the diseases observed in the Dog

2. Classify the bite wound and write about Post exposure prophylaxis
3. Describe the microscopic picture

For examiners

Sr. No Key Max

Marks
1 Rabies 01
2 Category III and requires Vaccine and 02
Rabies immunoglobulin
3 Eosinophilic inclusion body , Negri 01
body

8
For Candidate

Total Marks : 04 Time allotted : 04 Marks

1. Identify the Helminthic egg

2. Describe the Egg
3. What is the Infective stage of this parasite

For Examiners

Sr. No Key Max Marks

1 Trichuris trichiuria Egg 01
2 Barrel shaped, thick shelled and 02
possess a pair of polar Plugs at each
end
3 Embryonated Egg 01

9
For Candidate

Total Marks : 04 Time allotted : 04 Marks

Task

1. Identify the Given devise used in a serological technique

2. Name the serological technique in which it is used
3. Give FOUR clinical applications of this technique

For Examiners

Sr. No Key Max marks

1 ELISA Microtitre plate 01

2 Enzyme linked Immunosorbent Assay 01

(ELISA)
3 To detect Antigen / antibodies of Hepatitis B 02
virus, To detect antibodies of HCV Virus
and Cytomegalo virus , Hepatitis A virus
(0.5 marks each)

10
For Candidate

Total Marks : 04 Time allotted : 04 Marks

1. What is the Name of the above selective Medium 01 marks

2. It is used for isolation of which organism 01 mark
3. What is the composition of the medium 01 mark
4. What is the rationale behind production of yellow colonies on the medium
01 Marks

For Examiners

Sr. No Key Max marks

1 Thiosulphate citrate Bile Salt Sucrose Agar 01

2 Selective medium for Vibrio cholera 01

3 TCBS medium contains Yeast extract, 01
peptone, Nacl, Sucrose etc
4 Vibrio by fermentation of sucrose forms 01
yellow colour colonies

11
For Candidate

Total Marks : 04 Time allotted : 04 Marks

1. What is the Name of the above Medium 01 marks

2. The medium is used for isolation of which type of organisms 01 mark
3. What are the special components added to the medium and why? 02 marks

For Examiners

Sr. No Key Max marks

1 Sabourauds Dextraose Agar 01

2 Used for isolation of fungal pathogens 01

3 Sometimes chloramphenicol is added to 02
suppress the growth of the contaminated
bacteria and cycloheximide is added to
suppress the contaminated fungi

12