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    Biology Practical Experiment 8, 9 & 10

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    Ruby Kumari
    The document describes three experiments that demonstrate the key requirements for photosynthesis: 1. Experiment 8 shows that light is necessary for photosynthesis by covering part of a leaf with black paper and observing that portion did not turn blue-black when dipped in iodine unlike the exposed part of the leaf. 2. Experiment 9 shows that carbon dioxide is necessary by placing a leaf inside a flask containing potassium hydroxide, which absorbed the CO2, and observing that portion did not show blue-black color change with iodine. 3. Experiment 10 shows that chlorophyll is necessary by using a variegated leaf and observing only the green parts turned blue-black with iodine.

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    Biology Practical Experiment 8, 9 & 10

    Uploaded by

    Ruby Kumari
    612 views6 pages
    The document describes three experiments that demonstrate the key requirements for photosynthesis: 1. Experiment 8 shows that light is necessary for photosynthesis by covering part of a leaf with black paper and observing that portion did not turn blue-black when dipped in iodine unlike the exposed part of the leaf. 2. Experiment 9 shows that carbon dioxide is necessary by placing a leaf inside a flask containing potassium hydroxide, which absorbed the CO2, and observing that portion did not show blue-black color change with iodine. 3. Experiment 10 shows that chlorophyll is necessary by using a variegated leaf and observing only the green parts turned blue-black with iodine.

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    The document describes three experiments that demonstrate the key requirements for photosynthesis: 1. Experiment 8 shows that light is necessary for photosynthesis by covering part of a leaf with black paper and observing that portion did not turn blue-black when dipped in iodine unlike the exposed part of the leaf. 2. Experiment 9 shows that carbon dioxide is necessary by placing a leaf inside a flask containing potassium hydroxide, which absorbed the CO2, and observing that portion did not show blue-black color change with iodine. 3. Experiment 10 shows that chlorophyll is necessary by using a variegated leaf and observing only the green parts turned blue-black with iodine.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    612 views6 pages

    Biology Practical Experiment 8, 9 & 10

    Uploaded by

    Ruby Kumari
    The document describes three experiments that demonstrate the key requirements for photosynthesis: 1. Experiment 8 shows that light is necessary for photosynthesis by covering part of a leaf with black paper and observing that portion did not turn blue-black when dipped in iodine unlike the exposed part of the leaf. 2. Experiment 9 shows that carbon dioxide is necessary by placing a leaf inside a flask containing potassium hydroxide, which absorbed the CO2, and observing that portion did not show blue-black color change with iodine. 3. Experiment 10 shows that chlorophyll is necessary by using a variegated leaf and observing only the green parts turned blue-black with iodine.

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    Experiment 8

    I. Aim : To show that light is necessary for photosynthesis.


    II. Materials Required:
    i. Black strip of paper
    ii. A healthy green plant
    iii. Alcohol
    iv. Water
    v. Beaker
    vi. Water bath
    vii. Tripod stand
    viii. Petri dish and
    ix. Iodine solution

    III. Procedure:
    i. A healthy green plant is taken.
    ii. It is destarched by keeping it in darkness for about 24 hours so that the food
    made earlier is used up by the plant.
    iii. The next day the middle of one of the leaf is covered with a black strip of paper.
    iv. The plant is now exposed to sunlight for few hours.
    v. After a few hours the experimental leaf is removed. It is boiled in alcohol in a
    beaker over a water bath.
    vi. When the leaf becomes colourless, it is removed, and washed with water.
    vii. Now the leaf is sipped in iodine solution.

    IV.Observation:
    The portion of the leaf which was covered with the black strip of paper did not
    turn blue black when dipped in iodine whereas the rest of the leaf did.
    V. Inference:
    Photosynthesis does not occur in the absence of sunlight.
    VI. Precautions:
    1. The plant should be destarched properly
    2. Water bath should be used for boiling because alcohol is flammable.
    Experiment 9
    I. Aim : To show that carbon dioxide is necessary for photosynthesis.
    II. Materials Required:
    i. Conical flask
    ii. Split cork
    iii. Potassium hydroxide
    iv. A healthy potted plant.
    v. Iodine solution.
    vi. Alcohol
    vii. Tripod stand
    viii. Water bath

    III. Procedure:
    i. A healthy green plant with long leaves is taken.
    ii. The plant is kept in darkness for 24 hours
    iii. The next day a bottle containing potassium hydroxide is taken and its mouth
    is closed with a split cork.
    iv. One of the leaves of the plant is introduced through the split cork into the
    bottle. Only half the leaf is introduced.
    v. The plant and the bottle is exposed to sunlight for a few hours.
    vi. ‘The leaf which was introduced in the bottle is removed.
    vii. It is boiled in alcohol in a beaker which is kept in a water bath.
    viii. The leaf becomes colourless.
    ix. The colourless leaf is washed in water and dipped in iodine.
    VII.Observation:
    The portion of the leaf which is inside the flask does not give a blue black colour
    with iodine but the portion of the leaf which was outside gives a blue black
    colour with iodine..
    VIII. Inference:
    The Potassium hydroxide solution absorbed all the carbon dioxide inside the
    flask, due to which photosynthesis could not occur.
    IX. Precautions:
    i. The plant should be properly destarched.
    ii. The split cork should fit tightly in the mouth of the flask so that the apparatus
    is airtight.
    Experiment 10
    I. Aim : To show that chlorophyll is necessary for photosynthesis.
    II. Materials Required:
    1. A potted plant with variegated leaf.
    2. Pencil
    3. Notepad
    4. Iodine Solution
    5. Alcohol
    6. Petri dish
    7. Beaker
    8. Tripod stand
    9. Burner and vessel Containing water.

    III. Procedure:
    i. A healthy plant with variegated leaves is taken and kept in darkness for 24
    hours to destarch it.
    ii. It is exposed to sunlight
    iii. After 4-5 hours a few leaves are plucked.
    iv. A drawing of the leaf is made in a notepad to mark the green and non green
    parts.
    v. The leaves are then boiled in alcohol over a bath tub.
    vi. The leaves become colour less after sometime.
    vii. They are washed with water and dipped in iodine solution.
    IV.Observation:
    The green parts of the leaves turn blue black in colour.
    V. Inference:
    Green parts of the leaves have chlorophyll and carry out photosynthesis.
    VI. Precautions:
    i. The drawing of the leaf should be done carefully
    ii. The plant should be well watered.
    iii. The leaf should be boiled carefully sine alcohol is flammable.

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