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    Bioprocess Engineering 2

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    asg rost
    The document describes an experiment where trypsin hydrolyzed a substrate at different inhibitor concentrations. It provides hydrolysis rates for various substrate concentrations and inhibitor concentrations. It then asks the reader to determine the inhibition mechanism, as well as the kinetic parameters Km, Vmax, and Ki based on the data. A second experiment studied yeast growth in a chemostat at different flow rates and substrate concentrations. It asks the reader to determine the maximum growth rate (μmax) and saturation constant (Ks) based on Monod kinetics. A third problem considers cultivating a microorganism in one or two continuous stirred-tank fermenters (CSTFs) in series based on given parameters and asks whether four statements

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    Bioprocess Engineering 2

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    asg rost
    32 views1 page
    The document describes an experiment where trypsin hydrolyzed a substrate at different inhibitor concentrations. It provides hydrolysis rates for various substrate concentrations and inhibitor concentrations. It then asks the reader to determine the inhibition mechanism, as well as the kinetic parameters Km, Vmax, and Ki based on the data. A second experiment studied yeast growth in a chemostat at different flow rates and substrate concentrations. It asks the reader to determine the maximum growth rate (μmax) and saturation constant (Ks) based on Monod kinetics. A third problem considers cultivating a microorganism in one or two continuous stirred-tank fermenters (CSTFs) in series based on given parameters and asks whether four statements

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    this document is about bioprocess engineering

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    Bioprocess engineering 2

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    The document describes an experiment where trypsin hydrolyzed a substrate at different inhibitor concentrations. It provides hydrolysis rates for various substrate concentrations and inhibitor concentrations. It then asks the reader to determine the inhibition mechanism, as well as the kinetic parameters Km, Vmax, and Ki based on the data. A second experiment studied yeast growth in a chemostat at different flow rates and substrate concentrations. It asks the reader to determine the maximum growth rate (μmax) and saturation constant (Ks) based on Monod kinetics. A third problem considers cultivating a microorganism in one or two continuous stirred-tank fermenters (CSTFs) in series based on given parameters and asks whether four statements

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    32 views1 page

    Bioprocess Engineering 2

    Uploaded by

    asg rost
    The document describes an experiment where trypsin hydrolyzed a substrate at different inhibitor concentrations. It provides hydrolysis rates for various substrate concentrations and inhibitor concentrations. It then asks the reader to determine the inhibition mechanism, as well as the kinetic parameters Km, Vmax, and Ki based on the data. A second experiment studied yeast growth in a chemostat at different flow rates and substrate concentrations. It asks the reader to determine the maximum growth rate (μmax) and saturation constant (Ks) based on Monod kinetics. A third problem considers cultivating a microorganism in one or two continuous stirred-tank fermenters (CSTFs) in series based on given parameters and asks whether four statements

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    [ Problem (Modified from Katoh et al.

    , 2015, w/ permission)
    A substrate L-benzoyl arginine p-nitroanilide hydrochloride was hydrolyzed
    by trypsin with inhibitor concentrations of 0, 0.25, and 0.5 mmol/L. The hydrolysis
    rates, given in μmol L-1 s-1, are listed in the following table. Determine the inhibition
    mechanism and the kinetic parameters ( , max , and ) of this enzyme reaction.
    True or false?
    Substrate Inhibitor concentration (mmol/L)
    concentration
    0 0.25 0.50
    (mmol/L)
    0.1 0.79 0.57 0.45
    0.15 1.11 0.84 0.66
    0.2 1.45 1.06 0.86
    0.3 2.00 1.52 1.22

    1.( ) The inhibition mechanism is competitive inhibition..


    2.( ) The maximal rate max is greater than 10 mmol/(m³∙s).
    3.( ) The Michaelis constant is greater than 0.75 g∙mol/m³.
    4.( ) The inhibition constant is greater than 0.85 g∙mol/m³.

    g Problem (Modified from Dutta, 2008, w/ permission)


    A chemostat study was performed with yeast. The medium flow rate was
    varied and the steady-state concentration of cells and glucose in the fermenter
    were measured and recorded. The volume of the fermenter contents was 500 mL.
    The inlet stream was sterile.

    Flow rate, (mL/hr) Cell conc. (g/L) Substrate conc. (g/L)


    31 5.97 0.5
    50 5.94 1.0
    71 5.88 2.0
    91 5.76 4.0
    200 0 100

    Assuming that Monod kinetics are valid, find the maximum growth rate
    max and the saturation constant for this situation.
    A) max = 0.251 h-1 and = 1.52 g/L
    B) max = 0.251 h-1 and = 3.04 g/L
    C) max = 0.502 h-1 and = 1.52 g/L
    D) max = 0.502 h-1 and = 3.04 g/L

    g Problem
    Reconsider the system introduced in the previous problem. What should be
    the minimum flow rate to prevent washout of the cells?
    A) = 0.0814 L/hr
    B) = 0.161 L/hr
    C) = 0.312 L/hr
    D) = 0.505 L/hr

    [ Problem (Modified from Dutta, 2008, w/ permission)


    Suppose you have a microorganism that obeys the Monod equation

    dC X µ max CS C X
    =
    dt K S + CS

    where max = 0.6 hr-1 and = 4.0 g/L. The cell yield ( / ) is 0.58. You want to
    cultivate this microorganism in either one fermenter or two in series. The flow rate
    and the substrate concentration of the inlet stream should be 650 L/hr and 90 g/L,
    respectively. The substrate concentration of the outlet stream must be 6 g/L. True
    or false?
    1.( ) If we use a single CSTF, the size of the fermenter should be greater than 2000 L.
    2.( ) In operation of a single CSTF, the cell concentration of the outlet stream is
    greater than 42 g/L.
    3.( ) If the operation is carried out by two CSTFs in series, the substrate
    concentration in the outlet stream of the first fermenter will be greater than 12 g/L.
    4.( ) If the operation is carried out by two CSTFs in series, the sum of the two reactor
    volumes will be greater than 1800 L.

    2
    © 2020 Montogue Quiz

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