Abbott BIOLINE HIV 1/2 3.

0 JOB AIDE
For detailed instructions, refer to the product manual.

c INTERPRETATION OF
REMINDERS BEFORE TESTING Add 10 µL of Serum

1 2
Add 120µl of
or Plasma reagent buffer. RESULTS
30°C All components (20 µL) of Whole
must be brought Blood into REACTIVE
15°C to room specimen well
temperature (15- marked “S”. C
30 °C). 2
1
Check expiry
date and lot
number of NONREACTIVE
test kit.
C
Write test date, 2
kit data, and 1

3 4
Incubate for Interpret results.
patients’
10-20 minutes.
name/code on
worksheet. INVALID
DO NOT read test C
Prepare
results after 20 2
container for
minutes, late readings 1
infectious
wastes. can yield false results.

Department of Health
National Reference Laboratory – San Lazaro Hospital
STD AIDS Cooperative Central Laboratory (NRL-SLH/SACCL) Source: SD Bioline HIV 1/2 3.0 Quick Guide manual
Abbott Determine HIV – 1/2 JOB AIDE
For detailed instructions, refer to the product manual.

REMINDERS BEFORE Tear one strip Add 50 uL of

TESTING
30°C All components
must be brought
1 from the
right and
remove
cover.
2 serum or
plasma to
the sample
pad.
INTERPRETATION OF
RESULTS

to room
15°C
temperature (15-
30 °C).
NONREACTIVE
Lot #: 19938K200A
Check expiry Exp. Date: Feb 2024
date and lot
number of
test kit. REACTIVE
Write test Incubate at Read results at

3 4
date, kit data, room 15 minutes (to
and patients’ temperature for 60 minutes
name/code on 15 minutes. maximum).
worksheet. INVALID
Prepare
container for
infectious
wastes. INVALID
Department of Health
National Reference Laboratory – San Lazaro Hospital
STD AIDS Cooperative Central Laboratory (NRL-SLH/SACCL)
Source: Alere Determine HIV 1/2 Brochure
CHEMBIO HIV 1/2 STAT-PAK JOB AIDE
For detailed instructions, refer to the product manual.

Collect 5 uL of serum,
c INTERPRETATION OF
REMINDERS BEFORE TESTING

30°C All components

must be brought
1 plasma, or whole blood
using the Sample Loop.
Holding the sample
2 Add 105µl of
buffer.
RESULTS
REACTIVE
loop vertically, touch it
15°C to room
to the center of the
temperature (15- Sample (S) well.
30 °C).

Check expiry
date and lot
number of NONREACTIVE
test kit.

Write test date,

kit data, and

3 4
Incubate for 15 Interpret results.
patients’
minutes.
name/code on
worksheet. INVALID
DO NOT read test
Prepare
results after 20
container for
infectious
minutes, late readings
wastes. can yield false results.

Department of Health
National Reference Laboratory – San Lazaro Hospital
STD AIDS Cooperative Central Laboratory (NRL-SLH/SACCL) Source: CHEMBIO HIV 1/2 STAT-PAK™ Assay Product Insert