Observing Plant and Animal Cells: Purpose

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2.

2 Perform an Activity

Observing Plant and Animal Cells Skills Menu


Questioning Performing
In this activity, you will observe and compare a typical plant cell and a Hypothesizing Observing
Predicting Analyzing
typical animal cell. You will look at the organelles of each and identify Planning Evaluating
the major differences. The plant cell will be taken from an onion; the Controlling Variables Communicating
animal cell will be prepared slides of human cheek cells. After observing
these cells using a microscope, you will draw formal lab diagrams to
record your observations.

Purpose 4. Carefully place this membrane on the drop


Part A: To prepare a wet mount of onion cells, to of water.
study one cell using a microscope, and to draw and 5. Carefully lower the cover slip from one side to
label a diagram of the visible features. cover the sample.
Part B: To examine a prepared slide of human cheek 6. Observe the sample using low power. Focus
cells, to study one cell using a microscope, and to using the coarse-adjustment knob.
draw and label a diagram of the visible features. 7. Draw and label a sketch to show how the
individual cells fit together.
Equipment and Materials 8. Stain the specimen by putting one drop of iodine
• lab apron stain at one edge of the cover slip. Hold a piece
• disposable gloves of paper towel at the opposite edge to draw the
• clean microscope slide and cover slip stain across, under the cover slip (Figure 1).
• forceps or tweezers
• microscope
• dropper bottles of
• distilled water
• iodine stain
• onion
• paper towel
• prepared slide of human cheek cells
Figure 1 Staining an onion skin wet mount with iodine
Iodine stain is toxic. It can also stain skin
and clothing. Wear a lab apron and waterproof gloves 9. Observe the sample using medium power. Focus
when you use iodine in the lab.
skills handbook using the fine-adjustment knob.
2.D., 3.B.6.
10. Observe one cell. Draw and label a diagram of
Procedure the visible structures.
Part A: Preparing a Wet Mount 11. Rinse the slide and clean it as instructed.
of Onion Cells 12. If your teacher provides prepared slides of
1. Put on your lab apron and the disposable gloves. other plant cells, look at these and record your
2. Put one drop of distilled water on a clean observations.
microscope slide.
3. Using the forceps, peel the inner translucent
membrane from a small piece of onion.

34   Chapter 2 • Cells, Cell Division, and Cell Specialization NEL


Part B: Examining a Prepared Slide Apply and Extend
of Cheek Cells (g) If you looked at other plant cells in this activity,
13. Observe the provided slide of human cheek cells describe how they differed from an onion cell.
under the microscope using low power. Try to Give reasons for these differences. T/I

find an area of the slide where the cells do not (h) Most books state that plant cells contain
overlap. Focus on one cell using the coarse- chloroplasts. Based on your observations, is this
adjustment knob. statement correct? Support and explain your
14. Observe the sample using medium power. Focus answer. T/I

on one cell using the fine-adjustment knob. (i) Would you expect all animal cells to look the
15. Observe one cell. If necessary, use the high-power same? Explain your answer. T/I

objective. Draw and label a diagram of the cell


and any visible structures.

Analyze and Evaluate


(a) What structures were you able to identify in the
onion cells? T/I

(b) What structures were you able to identify in the


cheek cells? T/I

(c) What was the most obvious difference between


the two types of cells? T/I

(d) Are onion cells typical plant cells? Explain. T/I

(e) Suggest why onions and humans were used as


the source of cells in this activity.A

(f) What difference, if any, did you notice in the cells


before and after they were stained with iodine?
Why were you advised to stain the cells? T/I

NEL 2.2 Perform an Activity   35

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