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Version1

CERTIFIED REFERENCE MATERIAL

n-Alkane Standard Solution 16 components C10-C40 (all even) (ISO 9377-2-Mod)
50mg/l each of n-Decane [CAS:124-18-5] ; n-Dodecane [CAS:112-40-3] ; n-Tetradecane [CAS:629-59-4] ; n-
Hexadecane [CAS:544-76-3] ; n-Octadecane [CAS:593-45-3] ; n-Eicosane [CAS:112-95-8] ; n-Docosane [CAS:629-
97-0] ; n-Tetracosane [CAS:646-31-1] ; n-Hexacosane [CAS:630-01-3] ; n-Octacosane [CAS:630-02-4] ; n-
Triacontane [CAS:638-68-6] ; n-Dotriacontane [CAS:544-85-4] ; n-Tetratriacontane [CAS:14167-59-0] ; n-
Hexatriacontane [CAS:630-06-8] ; n-Octatriacontane [CAS:7194-85-6] ; n-Tetracontane [CAS:4181-95-7] in n-Hexane

Lot N: XXXXXX Ref N: F109781 Certification Date:XXXXXX

Barcode: XXXXXXXX

Component Certified Value* CAS Chemical Formula

and uncertainty [µg/ml]
n-Decane 49.76 ± 0.66 124-18-5 C10H22
n-Dodecane 50.10 ± 0.64 112-40-3 C12H26
n-Tetradecane 50.31 ± 0.56 629-59-4 C14H30
n-Hexadecane 50.81 ± 0.60 544-76-3 C16H34
n-Octadecane 49.94 ± 0.54 593-45-3 C18H38
n-Eicosane 49.95 ± 0.73 112-95-8 C20H42
n-Docosane 50.09 ± 0.74 629-97-0 C22H46
n-Tetracosane 50.06 ± 0.69 646-31-1 C24H50
n-Hexacosane 50.17 ± 0.64 630-01-3 C26H54
n-Octacosane 50.03 ± 0.67 630-02-4 C28H58
n-Triacontane 50.01 ± 0.55 638-68-6 C30H62
n-Dotriacontane 50.28 ± 0.69 544-85-4 C32H66
n-Tetratriacontane 50.15 ± 0.66 14167-59-0 C34H70
n-Hexatriacontane 50.22 ± 0.85 630-06-8 C36H74
n-Octatriacontane 50.43 ± 0.71 7194-85-6 C38H78
n-Tetracontane 50.13 ± 0.89 4181-95-7 C40H82

* WQP 5.15.1/2 The certified value was obtained gravimetrically and confirmed experimentally by GC/MS or HPLC

Density 0.6535 g/cm3 at 20°C

Starting Material Purity, Batch

n-Decane 98.9% (41365632)
n-Dodecane 99.5% (41319307)
n-Tetradecane 99.6% (41346068)
n-Hexadecane 98.0% (41313664)
n-Octadecane 99.9% (41318454)
n-Eicosane 99.9% (41351079)
n-Docosane 99.9% (41377789)
n-Tetracosane 99.9% (41375761)
n-Hexacosane 99.7% (41335727)
n-Octacosane 98.3% (41368008)
n-Triacontane 99.3% (41375600)
n-Dotriacontane 99.9% (41309957)
n-Tetratriacontane 99.9% (41341308)
n-Hexatriacontane 99.9% (41371534)
n-Octatriacontane 99.6% (41297452)
n-Tetracontane 98.4% (41368510)
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Storage Conditions: Store in a refrigerator at temperatures between 2°C to 8°C

Expiry Date: XXXXXXXXXX

Concept of Certification and traceability statement:
This certified reference material is produced by gravimetric measurement and dissolving the individual substances in n-Hexane .
The reported expanded uncertainty of measurement is stated as the standard uncertainty of measurement multiplied by the coverage factor k = 2, which for a
normal distribution corresponds to a coverage probability of approximately 95%. The standard uncertainty of measurement has been determined in
accordance with EA 4/02 and incorporates the uncertainties of the raw-material purity, the mass and the volume.
The metrological traceability is defined as the “property of a measurement result whereby the result can be related to a reference through a documented
unbroken chain of calibrations, each contributing to the measurement uncertainty”.
The metrological traceability is ensured through gravimetric measurement and dissolving of certified reference material/s (traceable to SI) from
laboratories/producers, accredited according to ISO 17034.
The measurement results are traceable to SI. All analytical balances used for the preparation of the solution are calibrated yearly under an in-house
procedure with class E1 and class E2 analytical weights, traceable to SI (DKD), and are checked daily.
Class A laboratory glassware is used.
The results from temperature measurement are traceable to SI. The thermometers used for solution's calibration are calibrated from an ISO 17025 accredited
laboratory. The ambient conditions are controlled with a hygrometer calibrated from an ISO 17025 accredited laboratory.
Both, purity of the starting materials and solvent, were checked using appropriate analytical instrument.

Intended use: For Laboratory Use Only

This CRM is intended for:
Calibration of TLC, GC/FID, GC/TCD, GC/ECD, GC/MS, GC/MS/MS, LC/UV, LC/MS and LC/MS/MS
Validation of analytical methods
Preparation of “working reference samples”
Detection limit and linearity studies
This statement is not intended to restrict the use for other purposes.

Instructions for the correct use of this certified reference material:

This CRM can be used directly or can be diluted in an appropriate solvent. Only a clean class A glassware should be used. Do not pipet from container.
Obtained concentration (in mg/l) after dilution is a result from the multiplication of certified value of CRM concentration and the CRM's volume used for
dilution and divided into the flask's volume used for dilution. For quantitative analysis, we recommend analyzing this mixture separately, without mixing it with
other solutions, to ensure accurate results for every compound.

Stability and storage:

This CRM is with a guaranteed stability until ±5% of the certified concentration for a period of 24 months. Stability is guaranteed of an unopened original
packaging stored, as written in the section: Storage Conditions. Even if the product is stable at normal laboratory conditions, in order to increase its stability,
we highly recommend it to be stored in a refrigerator.
The product should be used shortly after opening to avoid concentration changes due to evaporation. Warranty does not apply to a product stored after
opening.

Hazardous situation:
The normal laboratory safety precautions should be observed when working with this RM. Further details for the handling of this RM are available in a safety
data sheet.

Level of homogeneity
This solution was mixed according to an in-house procedure (MQP 5.13.1) and is guaranteed to be homogeneous.
To ensure sufficient homogeneity of the sample prior to use thoroughly mix by inversion or sonicate.

Names of certifying officers:

Laboratory: Margarita Dimitrova

Manager: Krassimira Taralova

This document QF 5.17.1/1 version 1 is designed and the certified value(s) and uncertainty(ies) are determined in accordance with ISO Guide 31, ISO Guide
35,and Eurachem / CITAC Guides

This certificate relates solely to the lot number given above.

All processes (including generating of this certificate) are completely controlled by the specialized Computer-Aided-Manufacturing (CAM) software.

This Certified Reference Material was produced under a quality management system that is:
- Registered to ISO 9001 Quality Management System (Lloyd's Register Quality Assurance Ltd Cert No 0039638)
- Accredited according to ISO/IEC 17025 – Testing (ANAB Cert No AT-1836)
- Accredited according to ISO 17034 - Reference Material Producer (ANAB Cert No AR-1835)

Additional Information
Analytical Data:
GC Conditions:
Column Agilent HP- 5MS 30m, 0.25mm, 0.25µm Oven Temperature Hold
Flow rate 0.7 ml/min Initial 35°C 7
Injector 250 °C 8°C/min 300°C 30
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Injection volume 1µl split (split ration 10:1)

Carrier gas He, constant flow

MS Conditions:
Transfer line 300°C Ionization mode EI
MS Source 230°C Mode SIM: m/z 71.1
MS Quad 150°C

Gravimetric Data

Component Purity Source Weighed Final Bulk/ Concen- Chemist

% Lot No quantity, g quantity, Standard tration ID
kg.10-3 Solution mg/kg
lot No
n-Decane 98.9 41365632 0.01886 2.1885 91659057 8523.0 AS
91659057 0.2919 32.673 XXXXXXXX 76.145 ER
n-Dodecane 99.5 41319307 0.0204 2.3321 91659330 8703.7 AS
91659330 0.2878 32.673 XXXXXXXX 76.667 ER
n-Tetradecane 99.6 41346068 0.02984 2.4598 91666666 12082.5 AS
91666666 0.2082 32.673 XXXXXXXX 76.997 ER
n-Hexadecane 98.0 41313664 0.02458 2.4094 91659354 9997.8 AS
91659354 0.2541 32.673 XXXXXXXX 77.755 ER
n-Octadecane 99.9 41318454 0.03323 2.4159 91666659 13741.0 AS
91666659 0.1817 32.673 XXXXXXXX 76.417 ER
n-Eicosane 99.9 41351079 0.0161 2.7221 91651051 5908.5 AS
91651051 0.4227 32.673 XXXXXXXX 76.440 ER
n-Docosane 99.9 41377789 0.0157 2.7645 91615893 5673.5 AS
91615893 0.4414 32.673 XXXXXXXX 76.649 ER
n-Tetracosane 99.9 41375761 0.01769 2.2899 91659323 7717.5 AS
91659323 0.3243 32.673 XXXXXXXX 76.603 ER
n-Hexacosane 99.7 41335727 0.0208 2.7183 91651013 7629.0 AS
91651013 0.3288 32.673 XXXXXXXX 76.774 ER
n-Octacosane 98.3 41368008 0.019 1.9561 91666635 9548.1 AS
91666635 0.262 32.673 XXXXXXXX 76.566 ER
n-Triacontane 99.3 41375600 0.0309 2.7759 91648839 11053.5 AS
91648839 0.2262 32.673 XXXXXXXX 76.529 ER
n-Dotriacontane 99.9 41309957 0.01782 2.5756 91634658 6911.9 AS
91634658 0.3637 32.673 XXXXXXXX 76.941 ER
n-Tetratriacontane 99.9 41341308 0.01919 2.2623 91666642 8474.0 AS
91666642 0.2959 32.673 XXXXXXXX 76.745 ER
n-Hexatriacontane 99.9 41371534 0.01271 2.5263 91666611 5026.0 AS
91666611 0.4996 32.673 XXXXXXXX 76.854 ER
n-Octatriacontane 99.6 41297452 0.01723 2.4691 91634610 6950.3 AS
91634610 0.3628 32.673 XXXXXXXX 77.177 ER
n-Tetracontane 98.4 41368510 0.01204 2.5366 91659347 4670.6 AS
91659347 0.5366 32.673 XXXXXXXX 76.708 ER
Time-->
Abundance

20000
40000
60000
80000
100000
120000
140000
160000
180000
200000
220000
240000
260000
280000
300000
320000
340000

5.00
10.00
n-Decane, T

15.00
n-Dodecane, T

20.00
n-Tetradecane, T

n-Hexadecane, T

25.00
n-Octadecane, T

30.00
n-Eicosane, T

n-Docosane, T

n-Tetracosane, T

35.00
n-Hexacosane, T

n-Octacosane, T

n-Triacontane, T

40.00
n-Dotriacontane, T
TIC: D407.50.HX.D\data.ms

n-Tetratriacontane, T

45.00
n-Hexatriacontane, T

50.00
n-Octatriacontane, T

55.00
60.00
n-Tetracontane, T

65.00