Sample Submission Guidelines

Thank you for choosing CD Genomics for your project. Our portfolio of services covers genomics, transcriptomics, metagenomics,
epigenomics, single cell sequencing, genotyping, microarray, and more. When considering any of our NGS services, please take
note of the sample requirements and instructions for packaging, labeling, and shipping your samples. This will help to guarantee the
success and quality of your project. You will need to provide the completed Sample Submission Form, clearly listing all the
samples you are providing for sequencing. Make sure the sample names on the form match the labels on the sample tubes. We
also ask that you submit an electronic copy of the form and any required QC data via email. Please label the top of the lid of each
tube - with a maximum of 4 alpha-numeric characters (for example: 4B01).

 Shipping Guidelines
 DNA Sequencing
 RNA Sequencing
 Single Cell Sequencing/Spatiotemporal Genomics
 Pre-made Library Sequencing
 Suggestions of Sampling

Contact CD Genomics for more inspiration and service content.

 Shipping Guidelines
1. When sending samples, the standard sample submission form provided by our company should be included (electronic version sent
by email, or paper version sent with the sample). Please carefully check and ensure that the sample name and quantity filled in the
information sheet are completely consistent with the sample name identification and sample quantity actually sent.
2. We suggest loading samples in 1.5 mL centrifuge tubes when possible. Please seal the tube with parafilm for transportation. In
order to prevent the centrifuge tubes from being crushed and broken during transportation (leading to sample loss) it is better to insert
sample tubes into 50 mL centrifuge tubes (or other rigid supports), which can also be packed with cotton, foam, etc.
3. Samples for large scale projects can be submitted in well-sealed 96-well semi- or fully-skirted PCR plates, or in strip-tubes with
individually attached caps. To prevent sample loss and/or cross contamination, we recommend tightly sealing all wells of the plate
with an adhesive sheet/foil. You can protect the plates or strip tubes in a sturdy box with plenty of cushioning. Sample shipments of
plates should be carried out on frozen blue ice or on dry ice, to ensure that the samples remain frozen through the shipment.
5. Do not write the sample name and other information directly on the tube wall or tube cover with an oil pen. It is better to write
sample names at the top and the side of each tube with black permanent marker.
6. If a DNA sample is stored in 70% ethanol, it can be transported at room temperature. If a DNA sample is dissolved in H2O or TE
buffer, it needs to be transported with ice packs.
7. RNA, cells, bacteria and frozen tissue samples should be stored in liquid nitrogen for quick freezing and then transported with dry
ice.
Note: The amount of dry ice and ice bags to be added during transportation depends on the season, the length of transportation, and
the thickness of the foam box.
8. For blood samples, we recommend 5-10 mL plastic anticoagulant blood collection vessels for loading. In order to prevent the blood
collection vessels from being damaged due to extrusion during transportation, it is necessary to wrap the body of each blood collection
vessel with bubble wrap and then place it in a rigid box.
9. Before sending the samples, the sample sender shall send a timely notice by mail, which should include the Sample Submission
Form and the tracking number (for timely registration and processing after the arrival of the samples). Since we are not available to
receive packages on weekends, please make sure your samples arrive on weekdays.
10. CD Genomics recommends that you use FedEx, DHL or UPS for the shipment.
DNA Sequencing Service Sample Requirements

DNA can be submitted in DNase-free Water, Elution Buffer, or 10mM Tris pH 8.0. DNA samples require an OD260/280 as close to
1.8~2.0 as possible. All DNA should be RNase-treated and should show no degradation or contamination. Ship with ice packs.
The total amount of DNA required depends on the specific application. Please refer to the Sample Requirements table for general
guidance.
NOTE: The concentrations listed below should be determined by fluorometry (e.g. Picogreen/Qubit/RiboGreen). If you do not have
access to a fluorometry-based assay and are relying on spectrophotometry (e.g. Nanodrop), increase concentrations by ~2 fold.
Recommended Minimum Minimum
Illumina Service Sample Type
Quantity Quantity Concentration
Whole Genome Sequencing Genomic DNA ≥ 500 ng 200 ng 10 ng/µL
Whole Genome Sequencing (PCR-free) Genomic DNA ≥ 1 µg 500 ng 20 ng/µL
Whole Exome Sequencing Genomic DNA ≥ 500 ng 100 ng 10 ng/µL
Complete Plasmid DNA Sequencing Plasmid DNA ≥ 1 µg 500 ng 20 ng/µL
Human/Mouse Mitochondrial DNA
Genomic DNA ≥ 200 ng 50 ng 5 ng/µL
(mtDNA) Sequencing-multiplex PCR
Human/Mouse Mitochondrial DNA
Genomic DNA ≥ 500 ng 200 ng 10 ng/µL
(mtDNA) Sequencing-probes
Plants tissue
Chloroplast DNA (cpDNA) Sequencing ≥ 5g
(Green tissue)
Viral Genome Sequencing Genomic DNA ≥ 1 µg 500 ng 20 ng/µL
Amplicon Sequencing Purified Amplicon ≥ 1 µg 500 ng 20 ng/µL
GBS/ddRAD Genomic DNA ≥ 300 ng 100 ng 10 ng/µL
BSA (Bulk Segregant Analysis) Genomic DNA ≥ 2 µg 500 ng 20 ng/µL
2b-RAD Genomic DNA ≥ 200 ng 50 ng 5 ng/µL
Metagenome Sequencing Metagenome DNA ≥ 500 ng 20 ng 5 ng/µL
16S/18S/ITS Sequencing Genomic DNA ≥ 100 ng 10 ng 1 ng/µL
SNP Microarray Genomic DNA ≥ 300 ng 100 ng 8 ng/µL
DNA Methylation Microarray Genomic DNA ≥ 1 µg 500 ng 8 ng/µL
SSR Genotyping Genomic DNA ≥ 500 ng 200 ng 10 ng/µL
 Genomic DNA ≥ 200 ng
Cell, PMBC ≥ 1×106
HLA Typing Blood ≥ 1 mL
Buccal Swab 2
Dried blood spot (DBS) 2 completely filled spots, each 10 mm in diameter
Genomic DNA ≥ 2 µg 20 ng/µL
PMBC ≥ 2 x105
TCR-Seq
Animal tissue ≥ 500 mg
Blood ≥ 0.5 mL

Long Read Sequencing

Recommended Minimum Minimum
Service Sample Type
Quantity Quantity Concentration
Whole Genome Sequencing (PacBio) Genomic DNA ≥ 3 µg 80 ng/µL
Whole Genome Sequencing
Genomic DNA ≥ 5 µg 20 ng/µL
(Nanopore)
Plasmid Sequencing (Nanopore) Genomic DNA ≥ 1 µg 500 ng 10 ng/µL
Genomic DNA ≥ 500ng 10 ng/µL
Tissue 1-3g 1g
Full-Length 16S/18S/ITS Amplicon Thallus 5g 3g
Sequencing Interstitial Fluid 3-5 mL 1 mL
Environmental Samples 3-5g 1g
Water filter membrane 3 1
Genomic DNA ≥ 2 ug 30ng/µL
Tissue 2g 1g
Long-Read Metagenomic Sequencing Interstitial Fluid 6-10 mL, sediment 2g 2 mL, sediment
Environmental Samples 6g 2g
Water filter membrane 6 2
 Epigenomics sequencing
Recommended Minimum Minimum
Service Sample Type
Quantity Quantity Concentration
MeDIP-Seq/hMeDIP-seq Genomic DNA ≥ 2 µg 1 µg 20 ng/μL
Genomic DNA ≥ 1 μg 200 ng 10 ng/μL
WGBS (Whole Genome Bisulfite
Cell ≥ 1 x106
Sequencing)
Tissue ≥ 50 mg
Genomic DNA ≥ 1 µg 20 ng 20 ng/μL
RRBS (Reduced representation bisulfite 6 3
Cell ≥ 5 x10 3×10
sequencing)
Tissue ≥ 30 mg
Genomic DNA ≥ 500 ng 50 ng 10 ng/µL
Targeted Bisulfite Sequencing Cell ≥ 1×106
Tissue ≥ 20 mg
oxWGBS-Seq Genomic DNA ≥ 3 µg 1 µg 30 ng/µL
oxRRBS-Seq Genomic DNA ≥ 2 µg 50 ng/μL
oxTBS-Seq Genomic DNA ≥ 1 µg 20 ng/μL
DNA 6mA-IP-Seq Genomic DNA ≥ 5 µg 20 ng/μL
Epityper Genomic DNA ≥ 1 µg
ChIPed DNA ≥ 10 ng 5 ng 1 ng/µL
7 5
ChIP-seq Cell ≥ 2 x10 1 x10
Tissue ≥ 500 mg
TF ≥ 5 µg 1 µg 20 ng/µL
6
DAP-seq Cell ≥ 5 x10
Tissue ≥ 500 mg 200 mg
6 4
Cell ≥ 1×10 5×10
ATAC-seq
Tissue ≥ 500 mg 200mg
Cell ≥ 1×105 5×10
4

CUT&Tag
Tissue ≥ 500 mg 200mg
RNA Sequencing Service Sample Requirements

RNA can be submitted in RNase-free water, RNA Stabilization Reagent, or 10mM Tris pH 8.0. All total RNA samples should be DNA-
free. RNA samples require an OD A260/A280 ratio ≥ 1.8, A260/230 ratio≥ 1.8 and a RIN ≥ 6. Ship with dry ice.
The total amount of RNA required depends on the specific application, please refer to the Sample Requirements table for general
guidance.
NOTE: The concentrations listed below should be determined by fluorometry (e.g. Picogreen/Qubit/RiboGreen). If you do not have
access to a fluorometry-based assay and are relying on spectrophotometry (e.g. Nanodrop), increase concentrations by ~2 fold.

Recommended Minimum Minimum

Service Sample Type
Quantity Quantity Concentration
Whole Transcriptome Sequencing Total RNA ≥ 3 μg 1 μg 20 ng/μL
UMI RNA-seq Total RNA ≥ 2 μg 1 μg 50 ng/μL
Low input RNA Sequencing Total RNA ≥ 20 ng 20 pg 1 pg/µL
Total RNA ≥ 500 ng 200 ng 20 ng/µL
6
mRNA Sequencing Cells ≥ 1×10
Tissue ≥ 50 mg 10 mg
Total RNA ≥ 2 μg 500 ng 50 ng/µL
6
Total RNA/LncRNA Sequencing Cells ≥ 2×10
Tissue ≥ 500 mg 100 mg
Total RNA ≥ 1 μg 200 ng 20 ng/µL
Small Sequencing Cells ≥ 2×106
Tissue ≥ 500 mg 100 mg
Total RNA ≥ 5 μg 2 μg 50 ng/μL
CircRNA Sequencing (linear RNA 6
Cells ≥ 2×10
digestion)
Tissue ≥ 500 mg 100 mg
Total RNA ≥ 4 μg 3 μg 50 ng/μL
6
Metatranscriptome Cells ≥ 5×10
Environmental Samples ≥ 1.5g
 Total RNA ≥ 1 μg
Bacterial RNA Sequencing
Cells ≥ 1x107
Degradome sequencing Total RNA ≥ 20 μg 15 μg 100 ng/µL
TCR-seq Total RNA ≥ 100 ng 10 ng/µL
Total RNA ≥ 5 μg 3 μg
7
CAGE-seq Cells ≥ 2×10
Tissue ≥ 500 mg 200 mg
6 7
Cells ≥ 5×10 -10
Ribo-seq
Tissue ≥ 400 mg 200 mg
Total RNA ≥ 1 μg 200 ng 10 ng/µL
Dual RNA-seq Cells ≥ 5×106
Tissue ≥ 500 mg 100 mg
Total RNA ≥ 100 ng 20 ng/μL
Exosomal RNA Sequencing Cell supernatants ≥ 15 mL
Serum, plasma ≥ 1 mL

Long Read Sequencing

Recommended Minimum Minimum
Service Sample Type
Quantity Quantity Concentration
Iso-Seq Total RNA ≥ 2 μg 600 ng 30 ng/µL
Nanopore Full-Length Transcripts
Total RNA ≥ 2 μg
Sequencing
Nanopore Direct RNA Sequencing Total RNA ≥ 15 μg
*
The Long Read Sequencing has high requirements for RNA quality, RNA samples are required to RIN ≥ 8.
 RNA Epigenomics Sequencing
Recommended Minimum Minimum
Service Sample Type
Quantity Quantity Concentration
IPed RNA ≥ 100 ng 40 ng 5 ng/µL
7
RIP-seq Cell ≥ 5×10
Tissue ≥ 500 mg 200 mg
IPed RNA ≥ 100 ng 40 ng 5 ng/µL
eCLIP-Seq Cell ≥ 3×107
Tissue ≥ 500 mg 200 mg
Total RNA ≥ 10 μg 2 μg 1 ng/µL
MeRIP (Methylated RNA
Cell ≥ 1×107
Immunoprecipitation)
Tissue ≥ 500 mg 200 mg
Total RNA ≥ 10 μg
7
RNA BS-seq (RNA Bisulfite Sequencing) Cell ≥ 1×10
Tissue ≥ 500 mg 100 mg
Single Cell Sequencing/Spatiotemporal Genomics

Minimum Quantity
Service Sample Type Recommended Quantity & Quality
& Quality
Single cell suspension, Fresh
ScRNA-seq 2×106 cells 1×106 cells
tissue
SnRNA-seq Snap frozen tissue ≥ 100mg(Please contact us for details)
10X Visium Spatial Transcriptome OCT embedded tissue, FFPE 6.5mm×6.5mm
Fresh living intact cells, not
suitable for samples that have
been fixed, stained, etc.; Cell suspension volume ≤1 μL, 1~500 single
Smart-Seq2
mammalian cells or other cells/sample, ≥3 biological replicates
eukaryotic cells without cell
wall structures
6
1×10 , Nuclei < 40 μm, complete nuclear 5
Single cell nuclei suspension 5×10
membrane, and the proportion of nuclei > 95%
6
≥1×10 Cell Viability>80%, Cell concentration 5
Single cell suspension 5×10
scATAC-seq 700~1200 cells/μL,
Fresh tissue 200 mg
Whole blood
≥ 5mL
anticoagulated with EDTA
3
1-10 , Single cells are stored in 1xPBS buffer
Cells 2+ 2+
(without Ca , Mg ), the volume is within 2 μL
Single Cell Genome Sequencing
DNA ≥ 0.5pg

Cell lines, Use 200µl PCR tubes to store cells (single or

primary cells, multiple cells), 5µl of lysate per tube, and no
ScWGBS
fresh tissue, more than 1µl of buffer when collecting cells.
frozen cells ≥3 biological replicates
 Cell lines, Use 200µl PCR tubes to store cells (single or
primary cells, multiple cells), 5µl of lysate per tube, and no
ScRRBS
fresh tissue, more than 1µl of buffer when collecting cells.
frozen cells ≥3 biological replicates

Pre-made Library Sequencing

The quality inspection method of the sizes and concentrations of the library is Qubit, Agilent bioanalyzer.

Platform Minimum Concentration Data Amount Volume Requirement

X<30G ≥15 μL
30G≤X＜100G ≥25 μL
Novaseq-PE150 2 ng/μL 100G≤X≤400G ≥50 μL
400G＜X＜800G ≥70 μL
800G ≥100 μL (additional 70μL for one more lane)
X<30GM ≥15 μL
30M≤X＜100M ≥25 μL
Nova- PE250 2 ng/μL
100M≤X<400M ≥50 μL
400M ≥100 μL (additional 70μL for one more lane)
HiSeq-PE150 1 ng/μL 1 Lane ≥10 μL
MiSeq-PE300 1 ng/μL 1 Flowcell ≥10 μL
 Suggestions of sampling
We all know that quality nucleic acid input equals quality sequencing data output , and so CD Genomics offers a complete line of
sample extraction services for any sample type. We can consult to provide effective solutions for any sample type.

Sample Type Quantity Recommended Shipping Method

6
Cell 1×10 cells Dry ice
Fresh Frozen Tissue 10 mg Dry ice
2
FFPE ≥ 4 FFPE slides, thickness 5~20 µm, area >150 mm Room temperature/Blue Ice
9
Viral Particles 5x10 Dry ice
Stool 100 mg Dry ice
Swabs 2 tubes/sample, 1 swap/tube Room temperature
Saliva 1 mL Dry ice/blue ice
Soil 100 mg Room temperature/Blue Ice
Water 50 mL Room temperature/Blue Ice
Plasma/Serum 10 mL Dry ice
2 mL Fresh blood in EDTA tube Blue Ice
4 mL Frozen blood in EDTA tube Dry ice
Whole Blood
2.5 mL Frozen blood in PAXgene tube Dry ice
3 mL Frozen blood in Tempus Blood RNA Tube Dry ice
500 µL (gDNA) Blue Ice
Bodily Fluids
500-10,000 µL (cell free DNA) Dry ice
Adherent cells
1) Observe the cells under the microscope and confirm that they are in good growth condition (the polymerization degree of normal
cells is about 80%).
2) Remove the culture medium, and quickly wash it twice with precooled PBS buffer solution.
3) Place the culture dish on ice, add an appropriate amount of pre cooled PBS into the culture dish. Scrape the cells on one side of
the culture dish with a clean cell scraper, and place the culture dish on the ice at an angle to make the buffer flow to one side. Pipette
the dissolved products into the precooled centrifuge tube, and after 500-600 g centrifugation, discard the supernatant.
4) Store at - 80 °C after liquid nitrogen quick freezing.

Cell Suspension
1) The cells in the culture bottle/dish were gently blown and mixed with a pipette gun, and transferred to a 15 mL centrifuge tube.
2) Horizontal centrifuge, centrifuge 400g~1000g at 4 °C for 5-10 minutes to collect cells and discard the supernatant.
3) Carefully wash the flake sediment twice with precooled PBS, place it on ice, and discard the supernatant.
4) Quickly freeze with liquid nitrogen, and storage at - 80 °C.

Animal tissue samples

Quick-freezing fresh tissue in liquid nitrogen
1) After fresh tissue is isolated, connective tissue, adipose tissue and other tissue types that are not required for the study shall be
removed immediately. If the tissue volume is large, try to cut small pieces (length, width and height ≤ 0.5 cm).
2) Quickly wash the tissue with precooled normal saline or PBS, and dry it with dust-free paper. Place it in a 1.5 mL or 2 mL
centrifuge tube for quick freezing and then store it at - 80 °C; If the sample size is large, please divide into aliquots.

Plant tissue samples

Quick-freezing fresh tissue in liquid nitrogen
1) After taking fresh plant samples, wash them with DEPC water immediately and dry them with dust-free paper.
2) Divide the plant tissue sample into 1-2 cm or 50-100 mg small pieces with scissors and place them in 2 mL, 15 mL or 50 mL EP
tubes.
3) Quickly freeze with liquid nitrogen, and storage at - 80 °C.

Bacterial sample
Quick-freezing bacteria liquid nitrogen
1) Collect an appropriate amount of bacterial liquid into a 50 mL centrifuge tube, centrifugate at a low speed (3000-5000g/10min) with
a 4 °C horizontal centrifuge to collect bacteria, and remove the culture medium as clean as possible;
2) Add 5-10 mL sterile water or PBS solution to wash twice, then transfer it to a 1.5 mL or 2.0 mL centrifuge tube, centrifuge at 1500
rpm at 4 °C for 10min, remove the supernatant, and retain the precipitated bacteria.
3) Quickly freeze with liquid nitrogen, and storage at - 80 °C.
Whole Blood sample
1) Samples should be collected in suitable collection media such as QIAGEN PAXGene solution following the manufacturer’s
instructions.
2) Collect > 500 μL blood per tube and prepare 2 tubes per sample.
3) Fresh samples should be stored refrigerated and shipped refrigerated, with a wet/blue ice package.
4) Frozen samples should be shipped frozen by overnight delivery, packed with protective bubble pad. Make sure dry ice surrounds
all sides of the specimens.
5) For whole blood sample volume in PAXgene blood RNA tubes, please ensure to collect > 2.5 mL blood, the yield should be
approximately > 3 μg.

Plasma sample
1) Use a 5 mL blood collecting vessel containing EDTA (purple head tube) to extract the whole blood. A total of 10 mL is required.
Gently mix up and down (the whole blood sample can be stored at 4°C when the centrifugation condition is limited, and the next
processing can be carried out within 1h).
2) At 4°C, centrifuge 1900g with a bucket type rotating head for 10min. Carefully aspirate the supernatant for plasma, and finally
discard 500 μL.
3) Centrifuge the obtained plasma again at 4 °C and 3000g for 15 min. Carefully aspirate, taking care not to disturb the sediment at
the bottom and side.
4) Sub-pack the plasma into EP tubes and freeze at - 80 °C before sample delivery to avoid repeated freezing and thawing.

Serum sample
1) Use a blood sampling needle and common serum tube (5 mL blood collecting vessel without anticoagulant) were used to collect
10 mL blood.
2) Let stand at room temperature for 30min, and at 4°C for 3-4h (blood clots can be seen at this time).
3) Use a pipette to aspirate the above pale yellow serum (about 4 mL) and transfer it into a 15 mL centrifuge tube. Centrifuge 3000g
at 4 °C for 15min. Carefully transfer the supernatant into a new 15mL centrifuge tube to maximize the quality of serum.
4) After centrifugation, freeze the serum at - 80°C within 15 minutes to avoid repeated freezing and thawing.

PBMC samples
1) We recommend collecting at least 7.5 mL of blood for PBMC isolation.
2) Resuspend PBMC cells in RPMI 1640 containing 10% fetal bovine serum. Adjust the PBMC concentration to 1 x 106–1 x 107 cells/
mL.
3) Centrifuge PBMC for a further 10–15 min at 350 x g. Remove and discard the supernatant.
4) Snap freeze the PBMC pellet in liquid nitrogen and store at - 80 °C for later DNA purification.
5) PBMC pellets must contain at least 106 cells in order to be processed. Pellets should be shipped on dry ice.
FFPE samples
1) We require approximately 80mg of tissue per sample.
2), After cleaning the tissue with PBS or physiological saline, completely immerse the tissue in formaldehyde fixing solution within
half an hour of sampling. Seal the tube with sealing film.
3) The fresh tissue should be fixed with formaldehyde for no more than 24h. If a pretreatment kit is used, the fixing time shall be in
accordance with the requirements of the kit. FFPE chips can be transported at room temperature.

Water body/membrane samples

1) Samples should be taken from at least 1-2 L of water or slightly turbid water.
2) Samples should be transported at low temperature.
3) Filter membranes with different pore size can be selected for filtration according to the research purpose.
4) After freezing with liquid nitrogen place sample in a sterile, RNAse-free centrifuge tube and ship on dry ice.

Urine
1) Use a 15 mL or 50 mL centrifuge tube to collect urine, 300g, centrifuged at 4 °C for 10min, and transfer the supernatant into a
clean centrifuge tube to avoid touching the bottom sediment.
2) 3000g, centrifuged at 4 °C for 15min, remove cells or cell fragments, retain the supernatant, and avoid touching the bottom
sediment. Samples can be frozen at - 80 °C prior to delivery to avoid repeated freezing and thawing.

Contact CD Genomics for more inspiration and service content.