Forensic Chemistry
Forensic Chemistry
Forensic Chemistry
FORENSIC SPECIALIST – A person that practices forensics such as; DNA Examiner, Ballisticians, Forensic Photographer and
ETC.
DR. EDMUND LOCARD – A French criminologist, Father of Modern Forensics, established the world’s first crime lab.
CHEMISTRY – study of different chemical principles, its properties; drugs, explosives. Compositions of drugs, blood, and
other biological samples.
FORENSIC CHEMISTRY – application of chemical principles in connection with the administration of justice.
FORENSIC CHEMIST – a person the practices Forensic Chemistry, to aid an investigation to solve legal problems.
- Stude of different toxics or poisonous substances that can affect a living organism.
o Arsenic
FORENSIC TOXICOLOGY – Use of toxicology to aid Medico legal investigations of death and poisoning.
PROF. MATHIEU ORFILA – Father of modern forensic Toxicology. He researches poisons’ effect to human body.
- LAFARGE CASE - Expert witness against Madam Marie Lafarge (after poisoning her husband, Charles Lafarge
with Arsenic)
- MARSCH TEST – to identify arsenic from the body of Charles Lafarge.
- BOOK: TRAITES DES POISONS TREATIES ON POISONS.
- Location where crime happens, space where physical evidence can be found.
o 2 TYPES OF CRIME SCENE
INDOOR CRIME SCENE
OUTDOOR CRIME SCENE
PHYSICAL EVIDENCE – Articles and materials that can prove that a crime really happened.
- SUFFICIENCY OF SAMPLE – enough and sufficiency of evidence or samples; weight, volume, or pieces.
o Inadequate samples can lead to inconclusive results, sufficient samples that can be used for
comprehensive testing and analysis.
- STANDARD FOR COMPARISON – comparative analysis
o Standard - known samples from a known individual
o Questioned sample – crime scene.
- MAINTENANCE OF INDIVIDUALITY – packed individually, prevent from contamination.
o Maintain its integrity and prevent contamination.
- LABELLING AND SEALING – avoid confusion.
o Preserved its evidentiary value.
CHAIN OF CUSTODY – written record of all the individuals who maintained unbroken control over certain items of
evidence collected from the crime scene. It aims to establish:
- GO SLOWLY – taking time to conduct the test, ensures thoroughness and accuracy of results.
- BE THOROUGH – ensure reliable and no mistakes are made, careful observations.
- TAKE NOTES – to ensure that all data is properly documented and can easily viewed and shared when
necessary.
- CONSULT OTHERS – consult others who have already handled a similar case.
- USE IMAGINATION – think ahead of the potential outcome, it increases your critical thinking skills.
- AVOID COMPLICATED THEORIES – Explain in layman terms, to easily understand.
FAILURE TO CONSIDER THE GOLDEN RULES WILL CONTRIBUTE TO DESTRUCTION OF EVIDENCE/SPECIMEN. THE
OTHER REASONS OF FORENSIC DISASTER ARE:
CHARACTERISTICS OF A FORENSIC CHEMIST “REPUBLIC ACT NO 754. ALSO KNOWN AS THE CHEMISTRY LAW”
- Examination of dangerous drugs as well as volatile substances (RA 9165 and PD 1619)
- Examination of explosive and/or explosive ingredients (PD 1866)
- Examination of firearms and paraffin casts to detect gunpowder nitrates and gunpowder residue (GPR)
- Conduct gunpowder examination of clothing.
- Examination of human internal organs
- Examination of fake products
- Conduct blood alcohol determination
- Urine and other bodily fluids
- Research and training
- Delivers lectures on forensic chemistry and toxicology.
- Provide expert opinion in court.
- Respond to queries of all investigative units.
- Conduct clandestine laboratory investigations.
- ORDINARY WITNESS – State facts based on their personal knowledge, nakita and narinig or naano ng
kanilang ibat ibang senses.
o Sight – visual
o Hearing – auditory
o Touch - cutaneous
o Taste – gustatory
o Smell - olfactory
- EXPERT WITNESS - opinions based on their skills, education, and expertise.
Blood – circulation tissue of the body. Blood is red because of hemoglobin. It is Opaque. Normal pH of our blood is 7.35-
7.45.
COMPOSITION OF BLOOD
LIQUID – 55%
RED BLOOD CELLS / RBC (ERYTHROCYTES) – carry oxygen from our lungs to the rest of our bodies.
WHITE BLOOD CELLS / WBC (LEUKOCYTES) – attacks destroy harmful microorganisms and thus serve as one of the
body’s defenses against infections.
- Water 90%
- Solid 10% (largely protein in nature and consist of albumen, several globulins and fibrinogen.
SERUM – is a straw – yellow liquid formed when clotted blood is allowed to stand sometime and the clot contracts.
- Blood is difficult to search for, the collection, preservation, packing and transportation of specimen
suspected to contain blood is another.
- Blood offers little resistance to decomposition.
- It undergoes a rapid change.
- To preserve blood, it will maybe add SODIUM FLUORIDE for a week at room temperature or indefinitely in a
refrigerator.
- 40-50 degrees centigrade is the ideal preserving temperature for blood and other perishable specimens.
- Collection of blood stains should be done as soon as possible.
BLOOD COLLECTION
FLUID BLOOD – use and galing siya sa mga violent crime, it can also be used in disputed parentage, hence in
DNA Testing also.
DRIED BLOOD OR BLOOD STAINS – this are collected from smooth surfaces, hard surfaces, glazed surface,
and bulky objects.
BLOOD EXAMINATION
- MICROSCOPIC TEST – directly ilalagay sa microscope to check if may mga components pa ng blood.
o BLOOD CORPUSCLES
Blood or not
Blood – human or animals?
Human maliliit ang cells, Animals malalaki.
- MICROCHEMICAL TEST– blood lalagyan ng chemical solution at may iba pang test na icoconduct then
ichcheck sa microscopic test.
o HEMOCHROMOGEN TEST (TAKAYAMA)
S: Hemochromogen solution
P: Pink/Salmon Pink/Feathery Crystals
o TEICHMANN’S TEST (HEMATIN CRYSTAL)
S: Glacial acetic acid
P: Brown/Large rhombic crystals
o ACETONE-CHLOR-HEMIN OR WAGENAAR TEST
S: Diluted acetic acid + Acetone
P: Brown/ Multi-shaped crystals
- SPECTROSCOPE TEST – idadaan sa spectroscope equipment, ichcheck yung wavelength
1. SOLUBILITY TEST – Recent blood shed is soluble in saline solution and imparts a bright red color.
2. HEAT TEST – blood stain when heated, will impart a muddy precipitate. (COAGULUM FORMATION -
pamumuo)
3. LUMINESCENCE TEST – Stains on dark fabric mixed with mud , paint, etc. Emit bluish white luminescence in a
dark room.
GROUP “O”
GROUP “A”
GROUP “B”
GROUP “AB”
ANTIBODY OR AGGLUTININ – cause agglutination or clumping together of the red blood cells.
SEMEN
SPERMATOZOON is a living organism, normally present in the seminal fluid consisting of the head, neck and tail. It is
from 50 to 55 microns in length.
2.5 to 5.0 cubic centimeters of semen per ejaculation. Semen contains 60 million spermato
zoa per cubic centimeter, 80% of which are motile after 45 minutes. After 3 hours not more
than 20% become abnormal in forms.
a. Inspection by means of the naked eye or with the use of the hand lens; grayish white to faint yellow,
map like contour with silvery appearance. Hardened with shiny borders.
b. Inspection by means of Ultraviolet light; bluish fluorescence.
Moisten a portion of the stained fabric with a very diluted hydrochloric acid solution. ½ to 5 hours (soak). Allow liquid
portion to dry on the slide.
- FLORENCE TEST
o S: Florence solution
o P: Brown
- BARBERIO’S TEST
o S: Picric acid
o P: Yellow
o M: needle shaped crystals
MICROSCOPIC EXAMINATION
- A dried spermatic fluid stain on the slide is stained with hematoxylin or methylene blue and counter-stained
with eosin.
- yung semen titingnan under microscope (pero di siya reliable, kasi may diseases na aspermia [walang
spermatozoa] at oligospermia [konti ang spermatozoa])
BIOLOGICAL EXAMINATION
- PRECIPITIN TEST – tinitingnan lang kung yung semen ay galing sa tao o sa animals.
- Seizure of apparel
- Should be no friction between apparel and the stain.
- Not to be rolled for transit.
- Thoroughly dried before packing.
- Fluid semen should be placed in a test tube. It may be preserved by a drop of 10% solution of formalin
during hot weather.
- GUNPOWDER OR PROPELLANT: the propellant is the PRIMARY PROPULSIVE FORCE in a cartridge which
when exploded will cause the bullet to be driven forwards the gun muzzle.
BLACK POWDER – A mixture of potassium nitrate (75%), sulfur (15%) and charcoal (10%)
SMOKELESS POWDER
o SINGLE BASE – contains either cellulose nitrate or nitroglycerine.
o DOUBLE BASE – powder is composed of both cellulose nitrate and nitroglycerine.
SEMI-SMOKELESS POWDER – Mixture of 80% of black and 20% of the smokeless powder.
GUNSHOT WOUND – open wound produced by the penetration of bullet slug within the tissues of the body.
CONTACT – gun muzzle pressed against, or within an inch or two of the body.
CLOSE DISCHARGE – 6 inches to 2ft.
DISTANCE DISCHARGE – over 2ft. or 3ft.
PARAFFIN TEST – test performed to extract the nitrates embedded in the skin.
- Conduct using hot wax. Opening the pores to EXTRACT the gunpowder nitrates.
DIPHENYLAMINE TEST OR DPA TEST (DERMAL NITRATE TEST, DPA, LUNG’S TEST, GONZALES TESTS)
S: DPA REAGENT
- To DETECT THE PRESENCE OF NITRATES, a test to determine whether a person fired a gun or not.
POSIBILITIES THAT A PERSON MAYBE FOUND NEGATIVE FOR NITRATES EVEN IF HE ACTUALLY FIRED A GUN
POSIBILITIES THAT A PERSON MAYBE FOUND POSITIVE FOR NITRATES EVEN IF HE DID NOT ACTUALLY FIRED A GUN
- Gunpowder particles may have been blown on the hand directly from the barrel of the gun being fired by
another person.
- Attempt to shield the body arising the hand in some instances result in the implanting of powder particles on
hand.
NATURAL FIBERS
o PLANT FIBERS
Cotton – Flattened, spiral twist.
Flax -
Ramie
Sisal
Jute – Smooth, parang may lines.
Hemp – Transverse lines, usually blunt. [parang putol]
Kapok
Coir
Abaca – smooth, uniform, tips are fines points.
PLANT - Sinusunog para malaman na plant fibers siya; burn rapidy, smells like burning leaves, steady flame.
o ANIMAL FIBERS
Silk – laway na parang special type of worms, structureless
Wool – sources of woolen fiber: alpaca, cashmere, mohair
ANIMAL – smells like burning feather/hair; burn very slowly, easily extinguish.
MAN-MADE FIBERS
o POLYESTER
o NYLON FIBERS
o ACRYLICS
o RAYONS
o ACETATES
GLASS is usually composed of oxides like SiO2 (silica), B2O3 (boric oxide), phosphorus pentoxide (P2O5). For
commercial use silica is the most important oxide.
X-RAY DIFFRACTION ANALYSIS – determine the type of pattern of glass, hinahanap yung non crystalline
substances ng glass, to understand the deep understanding and composition of glass.
PHYSICAL PROPERTIES EXAMINATION – cinocompare or dinedetermine yung iba’t ibang glass fragments para
malaman kung nanggaling sila sa iisang source
ULTRAVIOLET LIGHT EXAMINATION – nirereview yung iba’t ibang features na di kayang makita under the normal
lighting conditions.
RADIAL FRACTURE – fracture is like web cord, makikita sa opposite side (mas nauunang maform kesa sa concentric
fracture)
MOULAGE – Recover impressions found out at the crime scene that aids in solving the crime; ginagamit sa impressions
(Wheel, foot, tool, etc.)
CASTING MATERIAL – material which can be change from plastic or liquid state to solid condition is capable of use as
casting material.
METALLURGY – is the study of metals, inaanalyze natin yung mga composition ng metal which is crucial in
identifying the tool marking identifications, [firearm identification] It is applied to criminal investigations
such as in:
o Robbery
o Theft
o Hit and run cases
o Bomb and explosion
o Nail Examination
o Counterfeit coins
o Restoration of tampered serial numbers
METALLOGRAPHY – pero yung mga structures ng metals, deep understandings about the compositions of
structures of metals
PETROGRAPHY – branch of geology that studies soils and rock formations. (study of dust, dirt, safe
insulations, ceramics and other such materials, both natural and artificial)
TYPES OF SOIL
ALLUVIAL SOIL – ito yung ginagamit for crops or agriculture, matatagpuan malapit sa surrounding the bodies of
water.
COLLUVIAL SOIL – soil na may rock fragments, nakikita sa maraming bundok or history of landslides;
decompositions of metamorphic and sedimentary rocks, moved by gravity.
SEDENTARY SOIL – inactive, not migratory soil. Walang rock fragments, medyo malalambot and sandy and clayie.
COLLECTION AND SUBMISSION OF EVIDENCE – Soil in form of mud is usually recovered from shoes, slippers, clothes,
tires, tools and furniture. [match from the crime scene]
APPLICATION OF SOIL ANALYSIS SCIENTIFIC CRIME DETECTION – di pareparehas yung soil na meron sa crime scene.
DUST AND DIRT – A matter in the wrong place, study of such piece of evidence may often provide the investigatior with
clues as to the occupation of previous whereabouts of a person under investigation.
DUST – matter which is dry and in finely divided form. [galing sa air]
CLASSIFICATION OF DUST
DRUGS – pharmaceutical drugs; any chemical substance intended for used in the diagnosis or treatment, cure and
prevention of disease or symptoms.
- Substance that enters human body and can change the function or structure of the human organism.
KINDS OF DOSES
MINIMAL DOSE – Smallest amount of drug that produces therapeutic effects [nakakakapagpagaling]
TOXIC DOSE – Amount of drug that produces untoward effects or symptoms of poisoning.
STIMULANT (UPPERS) – A drug that excite the central nervous system; increasing alertness, decreasing fatigue, delaying
sleep, also impale appetite and cause weight loss.
DEPRESSANTS/SEDATIVES (DOWNERS)
- Drugs that have mild calming or sleep producing effect upon the central nervous system.
- Slowdown the body function.
- BARBITURATES, ALCOHOL.
OPIATES/NARCOTICS
- Group of drugs that are used medically to relieve pain but have a high potential for abuse.
- Substance that lessens pain or induces stupor.
- Plants chemical substances which affect the perception, sensation, behavior and produce hallucination on
the user.
HALLUCINATION – FALSE PERCEPTION (IBA YUNG NAKIKITA MO)
ILLUSION – FALSE INTERPRETATION (TAMA YUNG NAKIKITA PERO IBA YUNG INIISIP)
DELUSION – FALSE BELIEF/ GRANDEUR (GANDANG GANDA SA SARILI)
HAIR – analysis of hair provides much longer “testing period” giving a more complete drug use history
going back as far as 90 days.
TOXICOLOGY – a scientific study of poisons, especially their effects on the body and their antidotes.
ACTION OF POISONS
LOCAL – pag uminom ka poison, sasakit chan mo, which the poison may come into contact.
REMOTE – uminom ka poison natamaan utak mo; away from the site of application.
ACUTE POISONING – uminom ka poison, namatay ka agad; death within a short time.
CHRONIC POISONING – uminom ka ng poison pero di ka namatay, dahan dahan kang namamatay, unti unti
niyang natatamaan yung mga organs mo.
ACCIDENTAL – di mo naman talaga intention patayin yung tao [usually mga bata]
UNDETERMINED – di alam kung bakit namatay yung isang tao due to poison
EMBALMING – di mo na makikita yung body fluids ng tao, mahirap madetermine kung anong poison kinamatay.
PUTREFACTION – pag agnas ng katawan ng tao.
MINIMAL DOSE – Smallest amount of drug that produces therapeutic effects [nakakakapagpagaling]