LDLC Bx400 Tyb40 Rximmola Draft 01

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LDLc -D

Colesterol LDL
Enzimático colorimétrico. Líquido

PREPARACIÓN Y ESTABILIDAD
Ref. 41023. Reactivo listo para su uso. BX_400 / TYB_40 / RX_IMMOLA
Estable hasta la fecha de caducidad indicada en la
etiqueta, guardado en nevera a 2-8ºC y protegido de la luz.

CALIBRACIÓN
Utilizar un suero de calibración valorado por este método ó el calibrador incluido en el equipo, rehidratado con 1
mL de agua bidestilada. Estable 1 semana refrigerado o 5 semanas a –20ºC.

CONDICIONES OPERATORIAS
Introducir en la memoria del ordenador los siguientes parámetros:

CHEMISTRY PARAMETER

Method * Name HDLc-d Reagent Name Reagent (µL) Water (µL)


Print Name LDLc-d R1 LDLc-d 210
Sample SERUM R2 X Enable LDLc-d 70
Unit mg/dL
Assay Type Fixed Time Diluent Enable

Mesuring Points Decimal Points 2


Start End
1 43 76 Normal Range
Enable 2 - - Nº Range Name Min. Max.
1 * * *
Wave Lenght 2 * * *
Prim. 570 Sec x Disable 3 * * *
4 * * *
Normal
Sampling Sample (µL) Diluent (µL) LOW HIGH
Dilution 3 Technical (Conc.) * *
Rerun (High Prozone) Range (mAbs/10) -999999 999999
Dilution
Rerun (Low) SPT Wash Enable Reag. Name
Dilution Stirring Speed R1-Middle R2-Middle

Limit Checks Blank measurement


Duplicate Limit mAbs/10
Sensitivity Limit mAbs/10 Blank measurement Enable Reagent Bk for S1 (linear)
Linearity Limit % (mAbs/10)/min Meas. of reagent BK during run Next Run
Prozone Limit Reag. BK. meas. at calibration Reagent BK (system water)
SL1-S SL1-F Nº of measurement Duplicate
SL2-S SL2-F
SENS mAbs/10 Reagent Blank Limit Checks:
Duplicate Limit mAbs/10
x Absorbance limit
Reaction increase Instrument Factor
Limit * mAbs/10 A: 1.00 B: 0.00

Calibration: 2 linear point

REV.01 Ed. 2023/07

SPINREACT, S.A.U Ctra. Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
LDLc -D

LDL cholesterol
Enzymatic colorimetric. Liquid

PREPARATION AND STABILITY


BX_400 / TYB_40 / RX_IMMOLA
Ref. 41023. Both reagents are ready to use.
Stored at 2-8 ºC are stable up to the date of expiration as specified.
Once opened the stability is 4 weeks at 2-8ºC.

CALIBRATION

Use the specific serum included in the kit. Reconstitute the contents of the vial with 1 mL of distilled water.
The calibrator once reconstituted is stable one week at 2-8ºC or 5 weeks at –20ºC.

OPERATORY CONDITIONS

Introduce the following data in the computer:

CHEMISTRY PARAMETER

Method * Name HDLc-d Reagent Name Reagent (µL) Water (µL)


Print Name LDLc-d R1 LDLc-d 210
Sample SERUM R2 X Enable LDLc-d 70
Unit mg/dL
Assay Type Fixed Time Diluent Enable

Mesuring Points Decimal Points 2


Start End
1 43 76 Normal Range
Enable 2 - - Nº Range Name Min. Max.
1 * * *
Wave Lenght 2 * * *
Prim. 570 Sec x Disable 3 * * *
4 * * *
Normal
Sampling Sample (µL) Diluent (µL) LOW HIGH
Dilution 3 Technical (Conc.) * *
Rerun (High Prozone) Range (mAbs/10) -999999 999999
Dilution
Rerun (Low) SPT Wash Enable Reag. Name
Dilution Stirring Speed R1-Middle R2-Middle

Limit Checks Blank measurement


Duplicate Limit mAbs/10
Sensitivity Limit mAbs/10 Blank measurement Enable Reagent Bk for S1 (linear)
Linearity Limit % (mAbs/10)/min Meas. of reagent BK during run Next Run
Prozone Limit Reag. BK. meas. at calibration Reagent BK (system water)
SL1-S SL1-F Nº of measurement Duplicate
SL2-S SL2-F
SENS mAbs/10 Reagent Blank Limit Checks:
Duplicate Limit mAbs/10
x Absorbance limit
Reaction increase Instrument Factor
Limit * mAbs/10 A: 1.00 B: 0.00

Calibration: 2 linear point.

REV.01 Ed. 2023/07

SPINREACT, S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com

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