MO300

A Highly Flexible Research Grade Mass Spectrometer

AXIMA Performance
AXIMA Performance™ -
the Next Generation in MALDI CID MS/MS
A high performance MALDI-TOF-TOF Essential features providing
mass spectrometer utilizing state-of-the- confident results
art high-energy MS/MS, delivering
This next generation design MALDI system delivers all of
unparalleled flexibility, in a robust and
the features expected of an AXIMA series mass
reliable research grade system.
spectrometer:
• High resolution MS data in reflectron mode for more
accurate and confident analysis
• Uncompromized linear mass range and sensitivity
• Near-axis laser irradiation for enhanced ion
transmission and sensitivity in all modes of operation
• Advanced calibration algorithms with easy-to-use
software providing more accurate data
• Intuitive software incorporating data-dependent
workflows for achieving the maximum result with the
minimum user input, making it ideal for novice and
expert users alike
• Flexibility – this is a true workhorse. Polymers,
oligonucleotides, SNPs, metabolites, carbohydrates and
small molecules amongst others may all be analyzed
and processed.

Advanced MS/MS performance

The monoPULSE™ high performance, revolutionary ion
gate provides outstanding MS/MS precursor ion selection
resolution. When complex mixtures are analyzed, the
high resolution ion gate allows optimal isolation of ions
with similar nominal mass, affording successful
separation of MS/MS fragmentation patterns, producing
more and higher confidence identifications. Resulting
MS/MS spectra are easier to interpret and significant
database search hits are more readily achieved. The
• Highest energy collisions – CID with a laboratory frame high-energy collisions, together with the curved field
collisional energy of 20 keV reflectron (CFR) produce well-balanced, information-rich
• Outstanding sensitivity – uncompromised design, MS/MS data.
to ensure no MS/MS signal is discarded • The unique combination of the advanced curved field
• Low sample consumption – allowing many more reflectron design and the high-energy collision cell
MS/MS experiments to be performed on the same means that all fragment ions formed are detected,
sample spot regardless of where they are formed in the instrument.
Both LID and CID ions are accumulated into a seamless
• Variable repetition rate laser
spectrum providing the best possible MS/MS sensitivity.
• Optimal precursor ion selection resolution using
• The Low Mass Zoom™ (LMZ) feature allows rapid
revolutionary ion gating technology
enhancement of the region of the spectrum
• Manual or fully automated operation allowing the encompassing the immonium ions and isotopically
seamless analysis of as few or as many samples as labelled quantitative diagnostic ions.
required
• Gridless ion path, unique to leading MS/MS systems,
• Fully enabled for protein identification – designed to avoid unwanted ion scattering and ensure
Intellimarque™ software suite for automated the highest possible ion transmission.
data-dependent peptide mass fingerprinting and
• High sensitivity allowing multiple MS/MS acquisitions on
MS/MS of peptides with optional incorporated
the same sample spot through low sample consumption.
Mascot® database searching
• LC-MALDI software allowing confident identification of
off-line separated complex mixtures via automated
MS/MS
AXIMA Performance™ -
High Performance in a Robust and Flexible Design
A practical and ergonomic package
The compact floor-standing geometry is designed to
maximize laboratory space and allow easy installation and
servicing. The system is delivered ready to install, requiring
little assembly on site.
Attention has been paid to the important factors of cost of
ownership and lifetime operational costs, minimizing the risk
to the investor. The system has undergone the most rigorous
and extensive quality assurance program, to ensure high
reliability and robustness, in the best Shimadzu tradition.

As with all AXIMA series systems, industry standard

microtitre plate format MALDI targets are used for
convenience and simple experimental planning and tracking.
This allows seamless integration with a variety of robotic
sample handling systems.
Modular target adaptors permit the use of many different
sample target formats including FlexiMass™ and other
microscope slide formats.
All AXIMA systems can be fully supported throughout their
lifetime using sophisticated web-based service diagnostics
and realtime remote monitoring. Highly trained specialist
local service support engineers are available to install and
maintain AXIMA mass spectrometers. A wide range of service
contracts is available, catering for all budgets and
requirements, including IQ/OQ environments and high
throughput QA laboratories.
AXIMA Performance™ -
Excellence without Compromise
%Int.
~147300
High performance MS data
100

90
The AXIMA Performance demonstrates high resolution
80
and mass accuracy across a wide mass range, from
70
pharmaceutical compounds, through peptides to high
60

50 73954 mass proteins, to enable a variety of applications in the

40 research environment. A highly optimized linear mode
30 generates reproducible and sensitive results for very
20
high mass compounds and complexes, extending the
10
array of sample classes that may be analyzed. A typical
0
50000 100000 150000 200000
m/z
example of an intact high mass protein,
Immunoglobulin G, is shown (left).
Linear MS spectrum of Immunoglobulin G

Powerful precursor ion selection

The revolutionary monoPULSE ion gate allows the
selection of MS/MS precursors with industry-leading
resolution. Ions from complex mixtures or closely
associated neighbouring isotopic envelopes may be
isolated and subsequently fragmented. The gate
resolution of greater than 400 (FWHM) readily permits
the analysis of peptides with similar nominal mass,
even with overlapping isotopic distributions.

Efficient MS/MS from

high-energy CID
Once selected for MS/MS fragmentation, precursor
ions are subjected to true high-energy collisions with
the chosen collision gas, e.g. helium. The resulting
fragment ions are analyzed in the second TOF region
incorporating the curved field reflectron and detected
with the ultrafast MCP detector.
The high sensitivity means that good quality, usable
MS/MS spectra can often be obtained for low sample
amounts.
%Int.
100
The novel additional Low Mass Zoom (LMZ) scan allows
90
focus of the analysis to be directed around the low
80
mass region of the MS/MS spectrum. This feature can
70
be employed to increase confidence in MS/MS based
60
quantitation experiments and to aid in de novo
50
sequencing of unknowns by enhancing the immonium
40
ion region of the spectrum.
30

20

10

0
100 200 300 400 500 600 700 800 900 1000
m/z

Typical high-energy CID MS/MS spectrum

AXIMA Performance
AXIMA Performance™ -
Application-centric Solutions
Protein identification Biomarker discovery
Designed with the flexibility to adapt to users’ workflows: This exciting area encompassing clinical sample screening
from single sample manual acquisition to fully automated and profiling is comprehensively addressed using
data-dependent peptide mass fingerprinting (PMF) and automated acquisition methods and refined data
processing.
MS/MS for protein identification.
Data can be easily exported to third party software
• Peptide mass fingerprints are acquired and subjected to
packages to allow comparative experiments using a
an optional integrated Mascot® database search.
number of standard data formats including ASCII, mzXML
• User-defined acceptance limits for PMF-based protein
and mzData.
identification.
• Data-dependent MS/MS: using the results of the PMF
search, MS/MS may be performed on ions that were
Versatility
matched to the top ranked protein hit (confirmation
• The system’s flexibility and uncompromised linear mode
MS/MS), in addition to those that did not (investigation
performance lends itself to alternative applications such
MS/MS). Batch searching of these MS/MS spectra is then
as quality control.
performed automatically to provide additional and
higher confidence protein identifications. • Launchpad™ software offers a module, Oligo Analysis™,
for performing fully automated QC analysis of large
• Data may be reprocessed and resubmitted for database
numbers of oligonucleotides, peptides or small
searching at a later time to provide additional information.
molecules, complete with a report indicating the
presence or absence of a target compound, an estimate
LC-MALDI of the purity and occurrence of known contaminants,
adducts or truncated/extended analogues.
• The AXIMA Performance provides total support for
• Polymer applications are fully catered for using our
LC-MALDI based experiments.
unique polymer analysis software, Polymer Analysis™,
• The software suite allows the fully automated suitable for use with both polymers and copolymers.
acquisition of LC separated samples deposited onto
MALDI targets and the subsequent identification of
proteins via MS/MS of the peptides detected.
• The workflow automatically provides a provisional %Int. %Int.
100 100
intensity map of all sample spots across the target to 90
90 80
assess the distribution of peptides and identify the 70
80 60

position of the apex of the chromatographic peaks. 70

50
40

These are utilized to generate a candidate list and 60

30
20
10
MS/MS data is acquired for all discrete peptide ions. 50 0
80 100 120 140 160
m/z
40
• Exclusion lists are used to remove known contaminants
30
or high abundance peptides.
20
• All data is then subjected to an integrated Mascot® search. 10

• Low sample consumption allows multiple spectra to be 0

100 200 300 400 500 600 700 800 900 1000 1100
m/z
acquired from the same spot increasing the amount of
MS/MS data obtained. Typical MS/MS spectrum obtained by automated acquisition

%Int. %Int.
100 100

90 90

80 80

70 70

60 60

50 50

40 40

30 30

20 20

10 10

0
3480 3485 3490 3495 3500 3505 3510 3515 3520 3525 0
1500 1520 1540 1560 1580 1600 1620 1640 1660
m/z m/z

MS spectrum of Insulin B chain demonstrating resolution Peptide MS spectrum demonstrating attomole level sensitivity
>20,000 (FWHM)
 AXIMA Performance
t

Kratos Analytical Ltd.

Wharfside, Trafford Wharf Road, Manchester M17 1GP, UK
Phone: +44 161 888 4400 Fax. +44 161 888 4402
URL http://www.shimadzu.com/an/

For Research Use Only. Not for use in diagnostic procedures.

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Third Edition: January 2021, Printed in the UK MO300