Bile Acid Konelab Rev01

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APPLICATION PROCEDURE

KONELAB

BILE ACIDS
ENZYMATIC RECYCLING

Diagnostic reagent for quantitative in vitro determination of total bile acids in human serum or plasma on photometric systems

903115 5 x 50 ml 4 x 45 ml Reagent 1 1000 tests/kit


1 x 60 ml Reagent 2
903120 5 x 25 ml 4 x 22.5 ml Reagent 1 500 tests/kit
1 x 30 ml Reagent 2

Additionally offered:
903210 1 x 3 ml Bile Acids Standard
D98481 12 x 5 ml Control normal Diacon N
D98482 12 x 5 ml Control abnormal Diacon P

1. Reagent preparation:
The reagents are ready to use.
2. Instrument settings:
Test Definition: Rel. Error (%) *
Test type Photometric Min Max
Full name Bile ACIDS Response limit * *
On line name Bile Test flow
Result unit mg/dl
Blank NO Antigen excess NO
Number of decimals 1 Reagent 1 BILE1
Acceptance AUTOMATIC Reagent volume (µl) 150
Dilution 1 + 0 Disp with EXTRA Volume(µl) 20
Sample type Serum/plasma Sample Volume (µl) 3
Test in use YES Disp with EXTRA Volume(µl) 10
Low High Units
Test Limit 1 180 µmol/l Dilution with WATER
Initial Absorbance 0.0 2.5 A Incubation Time (sec) 180
Dilution limit * 180 µmol/l Reagent 2 BILE2
Secondary dil. 1 + 0 5 Reagent volume (µl) 50
Correction factor 1.00 Disp with EXTRA Volume(µl) 8
Correction bias 0.00 Incubation Time (sec) 60
Calibration parameters λ 1 (nm) 405 λ 2 (nm) 620
Calibration type LINEAR Curve type NONLINEAR
Repeat time (d) 0 Nonlinearity
Point/Calibrator 2 Conc (mmol/l) 3
Acceptance MANUAL % 20
Type of calibrator SEPARATE
Time (sec) 60
Calibrator id. WATER/CAL
Point & Inter
Concentration # Konelab 30/60 14/4.5
Konelab 20 9/7
Bias corr.in use NO
#) Data entry by the user
Abs. Error (mA) * **) Factor must be checked by a calibration serum

NOTE: These suggested instructions and instrument parameters are to be used in conjunction with the reagent package insert and the
instrument operation manual. Refer to these documents for complete instructions before performing the tests.

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