S5 Mid of Term Ii Biology Examination

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Name ……………………………………… …………………………………….

MID OF TERM II BIOLOGY EXAMINATION

Senior five.

P530/3.

Biology.

Practical.

July 2019.

2 ½ hours.

Instructions.

Answer all the questions in the spaces provided.

Use well sharpened pencil for drawings.

Untidy work shall not be marked.

For teacher’s use only.

Question. Marks.
1
2
Total

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1. A freshly killed specimen W is provided.
(a) (i) Classify it into the following taxa with two reasons each.

Phylum

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Reasons

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Order

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Reasons

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(b) Using a magnifying lens, examine the structures found on the head region of
specimen W.

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(i) Describe features on its head region used to classify the specimen into its
class. (03 marks)
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ii) Carefully severe off the head of the specimen from its body. Draw and

label the head from the anterior view. (5marks)

iii) Carefully cut the left compound eye together with the left antenna. Mount it
on a microscope. Using low power magnification, observe them.
Make an accurate drawing of the compound eye and the antenna from the
base. Do not label. (4marks)
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iv) Describe the units of compound eye as they appear under low power
objective lens of a microscope. (3 marks)
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v) Give the suitability of the units of the compound eye to its function. (3marks)

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(d) Dissect the specimen by cutting along the left edge of the cuticles to display
the structures on the ventral cuticle. Displace the alimentary canal to the left side.
Draw and label.(15 marks)

2. Solutions P1, P2 and P3 are extracts of different plant organs. Carry out the

following tests in tables 1, 2, 3 and 4 to determine the nutrients of each extract.

Record your tests, observations and deductions in the tables below.

(i) Benedict’s test

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Table 1

Test Observation Deduction.

P1

P2

P3

(ii) Biuret test.

Table 2

Test Observation Deduction.


P1

P2

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P3

(iii) Iodine test.

Table 3.

Test Observation Deduction.


P1

P2

P3

(iv) DCPIP test

Table 4.

Test Observation Deduction.


P1

7
P2

P3

(b) From your results suggest the plant part(s) from which the extracts were

obtained.

Explain your answer.

P1…………………………………………………………………………………………

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P2……………………………………………………...……………………………………

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P3…………………………………………………………………………………………

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( c) Remove the alimentary canal, and cut out the foregut and midgut. Put both the

mid gut and foregut in a mortar, grind into a fine paste and add 30 cm3 of water. Stir,

leave to settle and decant to obtain extract C. Divide the extract equally into three

test tubes labeled as 1, 2 and 3.

To test tube 1, add 3cm3 of solution P1, to test tube 2, and 3cm3 of solution P2and to

test tube 3, add 3cm3 of solution P3. Incubate the test tubes at 35 – 40oC for 20

minutes. After incubation, carry out the tests in Table 5 below on the contents of

each test tube to establish the effect of extract C on solutions P1, P2, and P3.

(i) Record your observations in the table.

Table 5

Test tube Observations after 20 minutes

Benedicts test Iodine test DCPIP

Extract C

3 cm3 P1

Extract C

3 cm3 P2

3.

Extract C

3cm3 P3

(ii) Explain your results of the tests with contents of


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Test tube 1.

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Test tube 2.

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Test tube 3.

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(iii) From your results in (c) (i), state two properties of the active substances in

extract C.

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