ISSN No.

2456-4400 Int J Med Lab Res 2022, 7(2):18-23

RESEARCH ARTICLE
RESEARCH ARTICLE

FORMULATION AND EVALUATION OF HERBAL LIPSTICK USING ROSA 'MISTER

LINCOLN'

Sai Datri A1, Lakshmana Rao A2, B.D.VVinayaki M3, Zakir Md4, Sri Snigdhanjani M5, Pavani N6

1 2 3-6
Assistant Professor, Professor and Principal and Students, V. V. Institute of Pharmaceutical Sciences,
Gudlavalleru.

Received: 23 June, 2022/Revision: 31 July, 2022 /Accepted: 07 August, 2022

ABSTRACT: Objectives: The objective of the present study was to synthesis a lipstick with natural color
pigment - anthocyanin which extracted from Rosa 'Mister Lincoln' and studies its color stability during storage
period. Materials and Methods: Different organic ingredients such as beeswax, Shreeji Wax, vanilla essence,
castor oil, olive oil, lemon juice and Anthocyanin extracted from Rosa 'Mister Lincoln' was used for the
formulation of herbal lipstick. Results: The Physico-chemical properties of the synthesized lipstick such as
spreadability, skin irritation test, and breaking point, surface anomalies, melting point, and perfume stability,
homogeneity and color uniformity were determined and compared with commercial lipsticks. The stability of the
synthesized lipstick found to be stable under dark condition while color loss was greater for lipstick in light
condition. Conclusion: Due to the low pigmentation from method, Anthocyanin is suggested incorporate in to
lip balm application. This study has proven that Anthocyanin could replace synthetic dye in cosmetics industry
for lip balm application.

KEYWORDS: Anthocyanin, Organic ingredients, Rosa 'Mister Lincoln' and lip balm.

INTRODUCTION:
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Int J Med Lab Res 2022, 7(2):18-23

pigments. Rose flowers grow in many different Herbal

products which having desirable physiological activity Anthocyanins [5-10] are responsible to produce red
like enhancing, smoothing appearance, healing, color in Rose’s which are belonging to the family of
conditioning properties due to the presence of herbal flavonoids. These anthocyanins are obtained from
ingredients. These are purely made by herbs and shrubs anthocyanins by adding sugars. Anthocyanins are
and thus are side effects free. These products provide
present in the cell vacuoles which are generally
nutrients and other useful nutrients to the body. Herbal
water-soluble pigments. Additionally, anthocyanin
lipsticks are the natural products that are prepared by
using herbal ingredients. These products moisturize pigments have been used as antibacterial agent.
and smoothen your lips and also impart color to lips by
using

Corresponding Author:
Dr Sai Datri Arige,
Assistant Professor,
V. V. Institute of Pharmaceutical Sciences, Seshadri Rao Knowledge Village,
Gudlavalleru,
cosmetics [1-4] are defined as the beauty places with different colors usually red in color.

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MATERIAL AND METHODS [10-15] Bees Wax 4.5 gm Provide hardness

and glossy
Selection of herbs: The rose flowers were selected as Shreeji Wax 3 gm Used as emollient
herbs in the formulation of herbal lipsticks due to their Castor Oil 6 ml Aid in blending
tackling and softening effect on lips. Collection of plant Olive Oil 1 ml Used as moisturizer
material: The rose flowers were collected from the local
Fragrance Few drops Used to provide
market and their petals were used in the formulation of fragrance
herbal lipsticks. Lemon Juice Few drops Used as anti-oxidant
Extraction of color pigment: Coloring agent Anthocyanin 2 ml Used as natural
Anthocyanin can be obtained from rose petals by milling (Rose) colorant
followed by pressing, filtration and obtained filtrate was
concentrated by using double boiling method.
Methodology [10-38]

The herbal lipstick using Rosa 'Mister Lincoln' flower was

formulated as per the following formula:

Table 1: List of ingredients for formulation of herbal

lipstick

Ingredients Composition per Importance

lipstick

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Int J Med Lab Res 2022, 7(2):18-23

Figure 1: Formulated lipsticks of rose flower RESULTS:

Method of preparation: Formulation of herbal lipstick It is very important to ensure the uniformity standard of
was carried out according to the general procedure of the produced lipsticks. Hence, the lipsticks produced
lipstick formulation. In this procedure, oil phases such as were evaluated on its spreadability, melting point,
olive oil and castor oil were heated in a beaker at 60º- breaking point, surface anomalies and skin irritation test
70°C by using heating mantel. Simultaneously, bees wax and the result were tabulated in Table 2.
and Shreeji wax were taken in another beaker and melted The spreadability test for F3, F5, F9 and F10 lipsticks
at 60º-70°c by using heating mantel. At the same were excellent as no fragment upon spreading with any
temperature, the contents of beaker containing melted deformation of lipstick was not observed. The melting
wax were added to the beaker containing heated oil point of lipsticks indicates the safe limit of storage. The
phases and thoroughly mix the contents until we get a standard melting point of a commercial lipstick is above
homogenous mixture. In a beaker, colored pigment, 50°C in order to remain its rigid structure and do not melt
vanilla essence and lemon juice as a preservative were in room temperature. Since the melting point of lipsticks -
added and mixed thoroughly. Above formed mixture F7, F8, F9, F11 and F12 determined are more than 50°C,
was added to the mixture of oils and waxes. The molten the synthesized lipsticks – F7, F8, F9, F10, F11 and F12
mixture was poured into lipstick moulds and kept aside at were consider achieved the requirement for safe limit
room temperature for solidification. Upon solidification storage. The breaking points of the synthesized lipsticks
lipsticks were collected from the moulds and packed in were fall on 105 g to 110 g which were slightly higher
the lipstick case. than the breaking point of a commercial lipstick of 95 g.
The ideal softening and rupture point in a lipstick is
Evaluation of herbal lipsticks: [10-38]
achieved by holding a good balancing between all
Evaluations of formulated herbal lipsticks were done by ingredients in lipstick formulation. The most tedious part
following methods: of formulating lipstick is finding appropriate ratio of wax
Melting point: The formulated herbal lipstick was in relation to oil in the lipstick formula. As wax in relation
taken in a crucible and subjected to double boiling in to oil increase, the melting point and the hardness of the
order to notify the melting point. By using lipstick will increase but at the same time, facilitating
thermometer, the temperature at which the lipstick sticks breakage. The results of melting point and breaking
starts to melt was noted. point of the synthesized lipstick - F9 show that the
Spreadabilty: The formulated herbal lipstick was formulation of lipstick was with appropriate wax to oil
tested for spreadability to examine whether the ratio. For surface anomalies test, no fungi and
lipstick producing greater friction or not during the crystallization were detected in the surface of lipstick.
rubbing process on the skin. Lastly, the synthesized lipsticks were applied on skin at
Breaking point: The breaking point test was back arms and skin behind the ear for 5 minutes to test for
conducted to determine maximum pressure withstand skin irritation as the skin area at those locations are the
by the lipstick during application on lips. This test was most appropriate location to test for skin irritation. The
performed by hanging the weights on formulated results showing no skin irritation was observed as all
herbal lipsticks until it breaks. ingredients used in the formulation are natural ingredients.
Surface anomalies: Surface anomalies were Besides the evaluation mentioned above, the lipsticks
evaluated by visualization of physical appearance of were evaluated on color uniformity by visual inspection.
lipsticks in order to determine surface irregularities. The color of the synthesized lipsticks – F7, F9 and F11
Skin irritation test: Skin irritation test for formulated are uniform and stable under room temperature. Lastly,
herbal lipstick was performed by applying it on skin for 5 the odors of all lipsticks are pleasant throughout the
mins to detect the presence of any side effects like itching, storage period.
redness and rashes on skin.

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These results of all the lipstick formulations indicated that CONCLUSION:

when compared with formulations of paraffin wax, the
This research work was concluded that the use of herbal
formulations with shreeji wax give best results. Moreover,
ingredients in the formulation of lipstick with diminished
among all the formulation, the formulation F9 meets all
or no side effects when compared to commercial lipsticks.
parameters in the commercial formulation standard.
It also provided the evidence that the formulated herbal
DISCUSSION:
lipstick matches with standard lipstick by showing
The present work formulation and evaluation of herbal positive results to the performed evaluation tests. Finally,
lipsticks [10-38] was intended to formulate a lipstick using
F1 F2 F3 F4 F5 F6 F7 F8 F F10 F11 F12
9
Spreadability E I I E U E U I U E E U I

Above 50°C 30 20 42 38 48 37 52 50 51 48 51 50
Melting Point
(°C)
Breaking point 95 60 45 50 55 90 48 100 98 110 110 111 110
(g)
Surface No No No No Yes No Yes No Yes No No Yes No
Anomalies
Skin No No No No No No No No No No No No No
irritation

Standard Anthocyanin Lipsticks

Evaluation Commercial
Parameters Lipstick
TABLE 2: Evaluation of Formulated Anthocyanin Lipsticks at room temperature

herbal ingredients with expect to reduce the side effects as we recommend this formula can be used for formulation
produced by the available synthetic ones. The prepared of standard herbal lipstick.
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