Workshop 8 PDT 3

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KEDAH MATRICULATION COLLEGE

BIOLOGY UNIT
DB035
SESSION 2014/2015 Workshop 8: Cellular Respiration-
Scheme

PART A: MULTIPLE CHOICE QUESTION

1.Which stage of aerobic respiration requires ATP?

A. glycolysis
B. Krebs cycle
C. electron transport chain
D. fermentation

2. Which of the following is not a product of fermentation?


A. CO2
B. O2
C. ethanol
D. lactate

3. The final electron acceptor in lactic acid fermentation is:


A. NAD+
B. pyruvate
C. O2
D. lactic acid
E. ATP

4. The net result of the breakdown of glucose in glycolysis and fermentation is the production
of:

A. 2 ATP
B. 36 ATP
C. 2 ATP and NADH
D. NADH, FADH2, and ATP

5. The final output of the Krebs cycle includes all of the following except

A. NADP
B. FADH2
C. ATP
D. CO2

6. The oxidation of glucose to two molecules each of pyruvate, ATP, and NADH is called
________ and occurs in the ________.
A. glycolysis; cytoplasm
B. fermentation; cytoplasm
C. the Krebs cycle; matrix of the mitochondrion
D. anaerobic respiration; cytoplasm
7. What substance is produced by the oxidation of pyruvate and feeds into the Krebs cycle?
Workshop 8: Cellular Respiration -
Scheme

A. pyruvate
B. acetyl-CoA
C. O2
D. CO2

8. What role does O2 play in aerobic respiration?

A. it combines with acetyl-CoA at the start of the Krebs cycle


B. it is given off as a by-product during the oxidation of pyruvate
C. it combines with H2O to help drive the formation of ATP
D. it is the final electron acceptor at the end of the electron transport chain

9. NADH is produced during

A. glycolysis
B. the oxidation of pyruvate
C. the Krebs cycle
D. all of the above

10. During chemiosmosis in aerobic respiration, protons are pumped


A. out of the mitochondria into the cell cytoplasm
B. out of the mitochondrial matrix into the outer compartment of the
mitochondria
C. out of the cell cytoplasm into the matrix of the mitochondria
D. out of the nucleus and into the mitochondria

PART B: STRUCTURE QUESTION

1. The diagram shows a series of reactions in which pyruvate is broken down and energy is

released.
Workshop 8: Cellular Respiration -
Scheme

a) Give the name for: [2 marks]

i. Reaction 1 : Linked reaction


ii. The series of reaction 2 to 5 : Krebs cycle

b) Name the compounds A, B, C and D. [4 marks]

A: Acetyl coenzyme A / Acetyl CoA


B: Citrate
C: α-ketoglutarate
D: Oxaloacetate

c) Name the compound X and Y. [2 marks]

X : Carbon dioxide
Y : NADH

d) Y then will transfer to another part of the mitochondrion for further reaction.

i. Name the reaction which involves Y. [1 mark]


Electron transport chain

ii. Name TWO end products of reaction in (e)(i). [1 mark]


Water and ATP (1/0)
Workshop 8: Cellular Respiration -
Scheme

PART C: ESSAY QUESTIONS

1. Explain how electrons from NADH and FADH2 flow through the electron transport chain
with the production of ATP. [10 marks ]

Mark
Electrons within NADH and FADH2 from the Krebs cycle enter the 1
electron transport chain which (consists of a series of molecules) embedded
in the inner membrane of mitochondrion.
Each molecule contains of one or several electron carrier. 1
Electrons are passed from one carrier to another at progressively lower 1
energy levels.
The carrier molecule that receives an electron undergoes reduction, and 1
the one losing electron is undergoing oxidation.
A small amount of energy is released during each transfer (through the 1
series of oxidation and reduction in electron transport chain).
Electrons from NADH enter the electron transport chain at NADH 1
dehydrogenase complex
Whereas electrons from FADH2 enter at a lower energy level, that is at 1
complex II to ubiquinone molecule.
The final electron carrier in the chain, transfer the electrons to oxygen to 1
form water.
The energy released by the electron transport chain is used to pump 1
hydrogen ions (H+) from the matrix into the intermembrane space.
This builds up a transmembrane electrochemical proton (H+) gradient. 1
When protons diffuse out from the intermembrane space through the ATP 1
synthetase into the mitochondrial matrix, ATP is synthesized from ADP
and Pi
Each NADH generates 3 ATP 1
And each FADH2 generates 2 ATP. 1
Total: 13
Max: 10

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