Cellular Respiration

How is energy transferred and transformed in living

systems?
Why
?
Living organisms display the property of metabolism, which is a general term to describe the
processes carried out to acquire and use energy. We know that people need to eat, and in our foods are
various kinds of nutrients that our cells use. One large group of nutrients in our foods is
carbohydrates, which supply our cells with glucose (C6H12O6). So the question is: How does the food
we chew and swallow fuel our cells?

Model 1 – Glycolysis
Cell membrane

NAD NADH

ADP ATP

ADP NAD ! = pyruvic acid (3 C) ATP NADH

= glucose (6 C)
= mitochondrion

= nucleus
NUCLEUS

1. Refer to Model 1.
a. What is represented by the hexagon?
GLUCOSE
b. How many carbon atoms (C) are in one molecule of glucose?
SIX
2. Refer to Model 1.
a. What is represented by the triangles?
PYRUVIC ACID
b. How many carbon atoms (C) are in one molecule of pyruvic acid?
THREE
3. In the process of glycolysis, what happens to glucose after it crosses the cell membrane
into the cytoplasm of the cell?

GLUCOSE IS BROKEN DOWN INTO PYRUVIC ACID (2)

Cellular 1
Respiration
 Read This!
Glycolysis occurs in the cytoplasm of cells and does not require the presence of oxygen. Therefore,
the process is anaerobic. It is the first step used by cells to extract energy from glucose in the form
of ATP. ATP can be directly used by cells.
4. Thinking about the number of carbon atoms in glucose and in pyruvic acid, explain why
there is one molecule of glucose on the left side of the arrow and two molecules of pyruvic
acid on the right side of the arrow.
ONE GLUCOSE MOLECULE HAS SIX CARBON ATOMS AND EACH PYRUVIC ACID
MOLECULE HAS THREE. THEREFORE, THERE HAS TO BE TWO MOLECULES TO CONTAIN
ALL SIX CARBON ATOMS.
5. How many ATP molecules are produced during glycolysis?
TWO
6. Hydrogen-carrying molecules are also produced during glycolysis. What is the symbol of
these hydrogen-carrying molecules?
NADH
7. Does glycolysis occur inside or outside the mitochondria?

OUTSIDE (CYTOPLASM)

Model 2 – Krebs Cycle

Inner
Mitochondrial matrix
mitochondrial
membrane
NAD+ FAD NADH FADH
NAD+ NADH

NAD+ NAD+ NADH

ADP NADH ATP
= pyruvic acid (3 C)
Outer mitochondrial
= carbon dioxide (1 C)
membrane
8. According to Model 2, what happens to pyruvic acid during the Krebs cycle?

IT IS BROKEN DOWN INTO THREE MOLECULES OF CO2

9. According to Model 2, where does the change identified in the previous question occur?

IN THE MITOCHONDRIAL MATRIX

2 POGIL™ Activities for High School

Biology
 10. Note the number of atoms of carbon in pyruvic acid and explain why three molecules of
carbon dioxide are produced.

EACH PYRUVIC ACID MOLECULE CONTAINS THREE CARBON ATOMS AND EACH
CARBON DIOXIDE MOLECULE CONTAINS ONLY ONE.

11. Considering that glycolysis produces two pyruvic acid molecules per glucose molecule,
how many total CO2 molecules will be produced from the complete breakdown of each
glucose molecule? Show a mathematical equation to support your answer.
SIX—EACH PYRUVIC ACID HAS THREE CARBONS AND TWO PYRUVIC ACID MOLECULES
ARE PRODUCED (3 X 2 = 6)

12. What two hydrogen-carrying molecules are formed during the Krebs cycle?
NADH and FADH2
13. Fill out the chart by looking back at the entire process of glycolysis and the Krebs cycle to
list the total number of ATPs and hydrogen-carrying molecules produced.

Process ATP NADH FADH2

2 2 0
Glycolysis

Krebs cycle 1 4 1
(1st pyruvic acid)

Krebs cycle 1 4 1
(2nd pyruvic acid)

Cellular 3
Respiration
 Model 3 – The Electron Transport Chain
Outer mitochondrial
membrane H+ H+
H+
e– = electron
= oxygen
Mitochondrial
matrix carrier proteins
e–
ADP ATP
NADH NAD+
e– H+
Inner mitochondrial
membrane FADH2 FAD H2O

14. What cell structure is the site for the electron transport chain?
THE INNER MITOCHONDRIAL MEMBRANE/CRISTAE
15. Label the carrier proteins in Model 3.
16. What substance do the carrier proteins transport across the inner mitochondrial membrane?
HYDROGEN IONS (H+)

Read This!
NADH and FADH2 molecules release hydrogen ions that are transported across the inner
mitochondrial membrane with the help of electrons. The result of these multiple processes is the
production of large amounts of ATP.
17. What high energy molecules are formed by the electron transport chain?
ATP
18. Refer to Model 3.
a. What atom accepts the hydrogen ion at the end of the electron transport chain?
OXYGEN ACCEPTS THE HYDROGEN IONS (“CLEANS UP!” FINAL ELECTRON ACCEPTOR)
b. What molecule is formed as a product of that acceptance?
WATER, H2O, IS FORMED AS A PRODUCT.
19. Formulate an explanation for why the events of the electron transport chain constitute an
aerobic process rather than an anaerobic process (like glycolysis).

THE ELECTRON TRANSPORT CHAIN IS AN AEROBIC PROCESS BECAUSE IT REQUIRES

OXYGEN TO COMPLETE THE PROCESS.

4 POGIL™ Activities for High School

Biology
Read This!
Remember that glycolysis produces two pyruvic acid molecules per glucose molecule along with two
of the hydrogen-carrying NADH molecules. Remember also that the Krebs cycle produces NADH as
well as another hydrogen carrier called FADH2. It is important to know that during the electron
transport chain, when each NADH gives up electrons and hydrogen ions, there is enough of a
potential energy change to make three ATP molecules. When each FADH2 gives up electrons and
hydrogen ions, there is enough of a potential energy change to make two ATP molecules.

20. Fill in the chart below to calculate the total amount of ATP produced from the breakdown of
each glucose molecule during the three steps of cellular respiration.

Number of H-carriers
Number of ATP produced from one glucose
produced from one molecule
glucose molecule NADH FADH2
2 2 0
Glycolysis
2 8 2
Krebs Cycle

Electron Transport Chain x3 x2

Total ATP Produced 4 30 4

Grand Total ATP produced (add all 3 columns above) 38

21. Look at the equation for cellular respiration and write in which stage of the process each
mol- ecule is either used or produced.
C6H12O6 + 6O2 → 6CO2 + 6H2O + 38 ATP
Used in Used in Produced in Produced in Produced in
ELECTRON KREB’S CYCLE ELECTRON GLYCOLYSIS – 2
GLYCOLYSIS TRANSPORT TRANSPORT KREB’S – 2
CHAIN CHAIN
E.T.C. - 34

22. Compare the ATP available to cells when oxygen is present versus when it is absent. How
might this help explain why brain and heart functions are so quickly affected when a person
cannot breathe?

SINCE THERE WOULD BE SO LITTLE ATP PRODUCED WITHOUT OXYGEN, THE

CELLS OF THE BRAIN AND HEART WOULD DIE AND THE FUNCTIONS WOULD STOP.

Cellular 5
Respiration
Extension Questions
Model 4 – Two Kinds of Anaerobic Respiration

pyruvic lactic
glucose acid acid

Fermentation (no O2 present in cell)

pyruvic alcohol +
glucose
acid CO2

23. What are the two substances that may be formed in anaerobic respiration?

LACTIC ACID OR ALCOHOL AND CO2

24. Recall that two molecules of ATP are formed during glycolysis. Neither fermentation
process shown above creates any more ATP. Knowing this, what would you predict about
the cellular energy available to organisms that carry out fermentation?

THEY MUST REQUIRE VERY LITTLE ENERGY, SINCE GLYCOLYSIS PRODUCES SO

LITTLE ATP AND FERMENTATION PRODUCES NO ADDITIONAL ATP.
25. Research the relationship between overexertion of muscles and the formation of lactic acid.
How does this relate to “the burn” felt during strenuous activity?
WHEN MUSCLES REQUIRE MORE ENERGY THAN CAN BE PRODUCED
AEROBICALLY, THEY SWITCH TO ANAEROBIC RESPIRATION.
THE LACTIC ACID PRODUCED BUILDS UP AND PRODUCES A BURNING SENSATION
IN THE MUSCLES.
26. What common foods involve the process of fermentation? Use your textbook or other
resource to make a list of the foods and the specific organisms used.

BREAD – Saccharomyces cervisiae AND OTHER YEASTS

CHEESE – VARIOUS FUNGI

YOGURT – VARIOUS BACTERIA

SAUERKRAUT – VARIOUS BACTERIA

VINEGAR – VARIOUS BACTERIA

WINE - YEASTS
6 POGIL™ Activities for High School
Biology