c.jenson Pillai Project Copy
c.jenson Pillai Project Copy
c.jenson Pillai Project Copy
BAKERY&CONFECTINARY DEPARTMENT IN
A HOTEL
Submitted in Partial Fullfilment of Requirement
for the award of
Submitted By,
C.JENSON PILLAI
Reg.No.229017370005
2022-2025
A SPECIALISED INSTITUTION
FOR SPECIALISED EDUCATION
HOTEL MANAGEMENT
Principal Director
ACKNOWLEDGEMENT
It is a pleasure to express my sincere thanks to those who helped me in this project work.
I express my deep sense of gratitude to all the other faculties for their involvement and help to
complete the project work very successfully.
DECLARATION
I hereby declare that the project entitled "A STUDY REPORT ON ROLE OF SPICES
AND HERBS IN INTERNATIONAL CUISINES DEPARTMENT TOWARDS IN
PRODUCTION " for the partial fullfilment of the bachelor degree of catering and hotel
administration my original work and that it has not previously formed the award of any Degree,
Diploma associate ship, fellowship or other similar report.
Date:
Thank you.
Table of Contents
Chapter-01-Spices…………………………………………………………1
1.1. Introduction .................................................................................................... 1
5
1.2. Important compounds in spices...................................................................... 2
1.3. Classification of spices .................................................................................... 3
Table 3. Based on the duration of the crops .......................................................... 4
Table 4. Based on the growth habit ....................................................................... 4
Table 5. Based on the plant part ............................................................................ 4
Table 6. Conventional classification ....................................................................... 5
Chapter-05-Other Effects…………………………………………….52
5.1. Insecticidal Effects ......................................................................................... 52
5.2. Anti- microbial ............................................................................................... 53
5.2.1. Antibacterial activity .................................................................................. 57
5.2.2. Antifungal activity ...................................................................................... 57
7
5.3. Anti-oxidant .................................................................................................. 58 5.4. Sensory properties
........................................................................................ 63
Chapter-06-Value Addition………………………………………….66
6.1. Importance of value-added products ........................................................... 68
6.2. Methods of value addition ............................................................................ 69
There are several methods available for value additions of the spices. .......... 69
6.2.1. Extending the shelf life........................................................................... 69
6.2.2. Polishing ................................................................................................. 73
6.2.3. Milling .................................................................................................... 73
6.2.4. Extraction of oil and oleoresins..............................................................74
6.2.5. Enzymatic treatments and fermentation ...............................................81
6.3. Packaging ......................................................................................................82
6.3.1. Selection of a suitable packaging material ............................................82
6.3.2. Different spice product packaging .........................................................84
6.3.3. Packaging methods ................................................................................85
6.4. Important areas to consider in order to improve the value-added
productions of spices ........................................................................................... 86
8
Chapter-01
Spices & Herbs
1.1. Introduction
Spices are different plant parts, including bark, flowers, roots, seeds, nuts, fruits, etc. which have been derived from
well-known plants. Dried or fresh leaves are not spice as they are considered as herbs [1]. Spices and herbs are most
commonly using to enhance the sensory properties of food since the ancient time as they contain compounds which
are responsible for unique seasoning properties [2]. Not only for this domestic cooking and food industry but also
for pharmaceutical, cosmetic industries spices are an essential part. Spices contain an ample amount of bioactive
compounds that are responsible for medicinal and antioxidative properties, and it helps to alter the shelf life of foods
by reducing the oxidation reactions and also act as an antiaging agent and reduce the risk of non-communicable
diseases as it is consumed by human [2].
Spices have a long history as it is running back hundreds of years. In 1555 BC, ancient Egypt classified fennel,
juniper, cumin, garlic, coriander as health-promoting spices. Also, there are records that onion and garlic were used
as a health-promoting agent by the laborers who constructed the Pyramids. Spices were essential ingredients in the
embalming process and used to cleanse the interior of the abdomen. Aromatic spices were also used as body
ointments in ancient Egypt. Spices which are having anti-microbial properties were used in surgeries for the
sanitization purpose.
In ancient India, spices and herbs were used even before about 1000 BC. Sickrooms, bed sheets of patients,
were fumigated with pungent and aromatic plant vapors for wounds they have applied sesame poultice. Ancient
Indians prescribed the usage of cardamom, ginger, black pepper, cumin, and mustard seeds to remove fat and to
cure urinary complaints. To increase the saliva flow and digestion clove and cardamom wrapped in betel nut leaves
9
were chewed after meals. In China, they used a spice called cassia, which is similar to cinnamon Arab merchants
purchased cinnamon from Sri Lanka and sold it in the Middle East and Europe. Later, Portuguese, Dutch, Spanish,
and English people did the same.
Also, in ancient times there were not any effective preservation techniques like refrigeration, freezing, UHT like
methods which we are using in the present. So, the preservation ability of spices made it even more valuable.
Volatile oils Aromatic, Antiseptic, Fungicidal, Irritant and stimulant Clove, Cinnamon
Piperaceae Pepper
Fabaceae Fenugreek
Cinnamon, Cassia
Lauraceae
Caesalpiniaceae Tamarind
Alliaceae Garlic
Myrtaceae Cloves
Orchidaceae Vanilla
Nutmeg, Mace
Myristicaceae
Cupressaceae Juniper berry
Perennial (More than two years) Curry leaves, Mint, Thyme, Oregano
Table 4. Based on the growth habit
11
Table 5. Based on the plant part
Size Example
Kernel Nutmeg
Tubers Galangal
Rhizome Turmeric, Ginger
Classes Examples
12
Chapter-02 Popular Spices and Herbs
1. Pepper
Types: Black pepper, White pepper, Long pepper
Family: Piperaceae
Special health benefits: Reduce the risk of cancer, diabetic, promoting digestion, increase the nutrition
absorption, treat ulcers, reduce inflammation [3, 4, 5].
13
Figure 1. Pepper
2. Cinnamon
Types: Ceylon cinnamon, Chinese, common cinnamon, cassia (C. cassia)
Family: Lauraceae
Major compound/s: Cinnamaldehyde (65%- 75%) , Eugenol Flavor and aroma: Pungent and sweet.
Special health benefits: Reduce cholesterol levels, Support insulin production, Protects against cancer,
Anti- inflammatory properties [6, 7, 8].
Figure 2. Cinnamon
14
3. Cloves
Botanical name: Syzygium aromaticum (synonym: Eugenia cariophylata)
Family: Myrtaceae
Special health benefits: Dental health, fighting bacteria, Protects liver, Anti- cancer properties, improve
digestion, Protects liver, Lowers high blood pressure, Regulates blood sugar, Treat acne [9, 10, 11, 12,
13, 14].
Figure 3. Cloves
4. Nutmeg
Botanical name: Myristica fragrans Houtt.
Family: Myristicaceae
Special health benefits: Supports oral health, Promote digestion, detoxification, Good for kidney health
(A good detoxifier), Brain booster, Removes bad breath, Pain reliver [15, 16, 17, 18].
15
Figure 4. Nutmeg
5. Mace
Botanical name: Myristica fragrans
Family: Myristicaceae
Special health benefits: Anti-carcinogenic, good for diarrhea, nausea and stomach pain [19].
16
Figure 5. Mace
6. Ginger
Botanical name: Zingiber officinale
Family: Zingiberaceae
Flavor and aroma: Pungent and aromatic. Flavour is a unique combination of citrus, soapy and musty
flavors.
Special health benefits: Flue, Anti-carcinogenic, for high blood pressure [20, 21, 22, 23].
Figure 6. Ginger
17
7. Garlic
Botanical name: Allium sativum
Family: Alliaceae
Plant part: Root bulbs
Major compound: Allicin (1-2%)
Special health benefits: Reduce blood pressure, Lower LDL levels, Anti-oxidant activity [24, 25, 26, 27].
Figure 7. Garlic
8. Fennel
Botanical name: Foeniculum vulgare
Family: Apiaceae
Major compound: methyl ester of trans anithole ( 50-90%), Fenchole (2-4%), Limonene
Special health benefits: Immunity booster, Anti-inflammatory effects, Manage high blood pressure ,
helps kidney function, good for muscle and bone health, Beneficial for menopause [28, 29, 30].
Figure 8. Fennel
18
9. Cumin
Botanical name: Cuminum cyminum
Family: Apiaceae
Special health benefits: Promote digestion, control diabetes, rich in iron, Promote weight loss [31].
Figure 9. Cumin
10. Cardamom
Botanical name:(Elettaria cardamomum);
Family: Zingiberaceae
Plant part: Seeds
Major compound/s: terpinyl acetate, linalool, 1-8-cineole
Flavour and aroma: Pungent, sweet and aromatic
Special health benefits: Anti- diabetic properties, Improve digestion [32, 33].
19
Figure 10. Cardamom
11. Chili
Botanical name: Capsicum spp.
Family: Solanaceae
Special health benefits: Pain relief, help weight loss, Enhance the Immunity, act as an antioxidant, anti-
carcinogenic agent , antiarthritic and analgesic properties [34].
12. Turmeric
Botanical name: Curcuma longa
20
Family: Zingiberaceae
Special health benefits: Improve liver function, Anti-inflammatory, relieve pain, Anti- carcinogenic [35,
36, 37, 38, 39].
13. Vanilla
Botanical name: Vanilla fragrans, vanillus planifolia, vanillus tahitensis
Family: Orchidaceae
Flavor and aroma: Highly aromatic, Sweet, bitter flavor. Highly fragrance.
Special health benefits: Treat intestinal gas and fever, Anti-aging due to anti- oxidant properties, Anti-
carcinogenic, cure dysmenorrhea [40, 41, 42].
21
Figure 13. Vanilla
14. Coriander
Botanical name: Coriandrum sativum
Family: Apiaceae
Special health benefits: Appetizer, diuretic, Anti- carcinogenic., Anti-aging [43, 44].
15. Mustard
Botanical name: Brassica juncea L.
Family: Brassicaceae
16. Fenugreek
Botanical name: Trigonella foenum-graecum L.
Family: Fabaceae
Special health benefits: Good for vitamin deficiencies like beriberi, To treat infections on the skin and
tissues [46].
23
Family: Apiaceae
Plant part: Leaves, Seeds
Major compounds: d-Limonene, Beta- pinene, limonene, selinene, flavonoids
Special health benefits: Have a low glycemic index (Good for diabetic patients), Contains vitamins (A, K,
C) and minerals (Potassium) , Reduce inflammation, Supports digestion, Anti carcinogenic effects,
Prevents cardiovascular diseases [47, 48, 49, 50, 51].
Family: Lamiaceae
Major compounds: alpha- linolenic acid, caffeic acid – (refer ncbi.nlm.nih.gov site to find more Details)
Special health benefits: Rich in omega 3, dietary fiber and proteins, Treat diarrhea and problems in
gastrointestinal tract, boost immunity [52, 53, 54].
24
19. Dill seeds
Botanical name: Anethum graveolens
Family: Apiaceae
Special health benefits: anti-inflammatory, diuretic, good for brain function, appetizer [55, 56, 57, 58, 59,
60].
20. Paprika
Botanical name: Capsicum annuum.
Family: Solanaceae
Special health benefits: Anti- inflammatory, Anti-bacterial, Anti- oxidant, improve digestion, Anti-
carcinogenic effects [61, 62].
25
Figure 20. Paprika
21. Saffron
Botanical name: Crocus sativus
Family: Iridaceae
Special health benefits: Good for nervous system, Heart health and menstruation, Diuretic, Immunity
stimulant [63, 64, 65, 66].
22. Sesame
Botanical name: Sesamum indicum
Family: Pedaliaceae
26
Major compounds: Sesamin , Sesamolin
Special health benefits: Anti- carcinogenic, Anti- aging, Alter sex hormones [67, 68, 69, 70, 71].
23. Garcinia
Botanical name: Garcinia gummi-gutta
Family: Clusiaceae
Special health benefits: Anti carcinogenic, Good for weight loss, anti-ulcer, Anti- oxidant, anti-
inflammatory, antibacterial, antiviral, anti-fungal properties [72, 73, 74, 75].
27
24. Tamarind
Botanical name: Tamarindus indica
Family: Fabaceae
Special health benefits: asthma, bacterial skin infections, wound healing properties, diabetes, diarrhoea, nausea,
promote digestion by stimulating saliva production, sore throat, good for liver, suitable for pregnancy related
vomiting, reduce urinary stones formation [76, 77, 78, 79, 89, 81, 82].
25. Shallots
Botanical name: Allium ascalonicum
Family: Liliaceae
Special health benefits: Anti-inflammatory properties, Good for arthritis, diarrhea, stomach pain, anti-carcinogenic,
Good for kidney health, anti- oxidant [83, 84, 85, 86, 87, 88].
28
Figure 25. Shallots
26. Oregano
Botanical name: Origanum vulgare
Family: Lamiaceae
Special health benefits: diarrhea, promote digestion, Treat asthma, stomach aches , Anti- inflammation,
boost immunity, manage diabetic and cardiovascular diseases [89, 90, 91, 92, 93, 94, 95].
27. Thyme
Botanical name: Thymus vulgaris
Family: Lamiaceae.
29
Major compound: Thymol
Special health benefits: Anti- inflammation, wound healing, Treat cough [96].
28. Sage
Botanical name: Salvia
Family: Lamiacea
Major compound:1,8-cineole, camphor, borneol, bornyl acetate, camphene, α- and β-thujone, linalool
Special health benefits: Anti- carcinogenic effects, inflammations, depression, digestive system, nervous
system [97, 98, 99, 100, 101].
29. Rosemary
Botanical name: Rosmarinus officinalis L
Family: Lamiaceae
Major compound: 1,8-cineole, camphor, and α-pinene Flavor and aroma: Bitter sweet flavor.
30
Special health benefits: Anti- carcinogenic [102].
30. Parsley
Botanical name: Petroselinum crispum
Family: Apiaceae
Special health benefits: Anti- carcinogenic, anti-inflammatory, Protects against diabetes, Diuretic effect,
Improve liver health [103, 104, 105, 106, 107].
31
31. Basil
Botanical name: Ocimum basilicum L
Family: Lamiaceae
Major compounds: Methyl chaviole and Linalool. (Also contains estragole, eugenol, cintronellol,
apigenin, limonene, methyl cinnamate, ursolic acid, 1,8-cineole, α-terpinene, β-sitosterol, anthocyanins,
p-coumaric acid, rosmarinic acid, carvacrol)
Special health benefits: Reduced stress, anxiety, Improved immune responses, Anti-aging effects [108,
109, 110].
Family: Lauraceae
Major compound: 1,8-cineole (Also contains α-terpinylacetate, cinnamtannin, sabinene, and α-pinene)
Special health benefits: Improves Digestion, Anti-inflammatory Activity, Prevent Cancer, Reduce
Anxiety & Stress, Anti-diabetic [111, 112, 113, 114].
32
Figure 32. Bay leaves
Family: Rutaceae
Special health benefits: Anti-Diabetic, Stimulating The Salivation Process and Stimulates Digestive
Enzymes , Anti-Inflammatory Activity, Anti-Oxidation, Wound Healing Effect, Eye Sight Improvement
[115, 116, 117].
34. Mint
Botanical name: Mentha L.
Family: Lamiaceae
33
Special health benefits: gastrointestinal disorders like flatulence, indigestion, nausea, vomiting, anorexia, freshness
in breath [118].
34
nutrition through many pathways. Generally, spices are adding into the meals as small quantities, but they
additionally give nutritional value other than enhancing food’s palatability.
• Contain protein, fat and fibres.
• Presence minerals such as calcium, potassium, sodium, iron
Fennel and phosphorus.
• Rich in vitamins such as thiamine, riboflavin, niacin and
vitamin C. [132, 133]
• Rich source of vitamin C.
• Other than that rich source of vitamin A, B, and E.
Chilli
• Good source of potassium, magnesium, iron, calcium,
pepper
phosphorus. [134, 135, 136, 137]
35
• Vitamin A, B complex, K, E, C and folic acid presence in
thyme. [148,
149]
• Rich in fat.
• Other than that presence in protein, dietary fibre, energy,
carbohydrate.
• No cholesterol found in Mustard.
• Rich in vitamin E.
Mustard
• Thiamine, folate, pyridoxine, niacin, Riboflavin, vitamin C
presence in respectively.
• Low in vitamin A.
• Rich in selenium, manganese andiron.
• Low in sodium and potassium. [150]
• Contain protein, carbohydrate sugar fat and fibre.
•
Garcinia • Iron, calcium, ascorbic acid and oxalic acid contain in
Garcinia. [151]
• rich in carbohydrate, starch, fat, protein, crude fiber and reducing sugar.
• Cardamom rich in vitamin C, B6, thiamine, riboflavin and niacin.
• Zinc, copper, iron, sodium, manganese, potassium, calcium, magnesium, phosphorus respectively.
Cardamo m [126, 127, 128]
Cinnamon
36
• Contain energy, protein, carbohydrate and fat.
• Potassium, calcium, phosphorus, sodium, magnesium presence as minerals.
• Rich source of vitamin C, thiamine, riboflavin, niacin presence as vitamins. [131]
Dill
Different parts of spices such as bark, seeds, flower buds, roots are shown medicinal properties. The most common
medicinal properties can be listed as, antithrombotic, anti-carcinogenic, antiarrhythmic, anti-rheumatic,
antisclerotic, lipid-lowering actions and gastroprotective. Other than that, spices can provide protection against
radiation and also inhibit the oxidation of protein glycation and low-density lipoprotein (LDL). Most of the time,
traditional ancient people used to utilize various kinds of spices to overcome a wide range of health problems and
diseases, although they didn’t know the scientific background. But nowadays there are proven results of the
37
beneficial effects of these natural sources. It is possible to add spice for our day to day meal which will help to heal
some of the common diseases as well as syndromes and to increase the disease resistance.
Using spices as a source of medicine for serious health issues highly preferable as they have been used for many
years without toxic effects. They are considered safe and have fewer side effects when compared with chemical
medicines [152]. Also, most of them are highly available and less expensive [153]. Also, it is always better to avoid
those health issues by using spices and herbs like natural sources without going into synthetic drugs with other side
effects after the health issue occurs. Proper knowledge about the medicinal properties of the spices is essential for
that.
Even the World Health Organization supports to develop treatments by using these natural sources to reduce
the side effects [154].
4.1. Anti-carcinogenic
Cancer is one of the most common non-communicable diseases which is having high mortality, and there are many
types of it. Commonly lung, rectum, colon, and prostate cancers are seen among men and breast, colon, rectum and
stomach cancer are seen among women [155]. Normally, there are cancer cells in everyone’s body by the
transformation of normal cells into cancer cells through many stages. But our immune system controls the growth
of them. Cancer which is known as the growth of tumour cells uncontrollably and undesirably in high amounts by
the cell division can occur mainly if the immune system is weak or if we increase the number of tumour cells in our
body by stimulating their growth. This stimulation can occur by various environmental factors and carcinogenic
compounds we consume in our day to day life.
There are different stages in the development of cancers. The first stage is known as the initiation and there will be
a reaction between cancerproducing substance, and DNA of tissue cell. This stage is dominant, and the development
of cancer can be easily controlled at this stage. It takes a long time to go up to the second stage, and at this stage
also it can be controlled easily. But it is very hard to control it at the third, and final stages and it spreads rapidly at
these stages [156].
In most cases, the symptoms are showing when it is too late. So, it is very important to consider about controlling
the growth of cancer cells before those irreversible stages occur. Also, most of the time, the synthetic drugs used to
treat cancer will the damage healthy cells as well. That is the reason behind the many side effects occurs after those
treatments. There are natural sources, including many spices which can reverse or prevent the development of cancer
cells.
• Spices that can be used for this condition: garlic, turmeric, cinnamon, clove, black pepper, fenugreek, ginger,
onion, mustard, thyme, fennel, saffron, coriander, chilli pepper.
Garlic, onions reduce stomach cancer risk [157]. Rosemary, sage contains carnosic acid and rosemary acid (RC-
18), which reduce the risk of cancer [158, 159]. Curcumin which is mainly found in turmeric, is good for colon
cancer, breast cancer [160, 161]. Saffron is also listed as an agent for anticancer drugs [162]. The gingerol, which
is containing in ginger, also helps to control the growth of tumors, breast cancers, ovarian cancers [163, 164, 165].
4.2. Anti-diabetic
Diabetes is a metabolic syndrome, and it will not allow using the energy of the food we consume properly. It either
happens if the pancreas is not secreting the required amount of insulin or if the insulin secreted by the pancreas does
not work as required. (Insulin resistance) So, there are two types of diabetes.
Type 01- Insulin-dependent diabetes mellitus (IDDM). It is only seen among 5-10% of diabetes patients. It most
commonly happens due to genetic reasons and autoimmune; environmental factors also increase the risk of this type
of diabetes.
38
Type 02- Non-insulin dependent diabetes mellitus (NIDDM). It is seen among 90-95% of diabetes patients. Obesity
increases the risk of these type of diabetes, and this type is easy to control by controlling the diet, exercise and
medications. It can also occur during pregnancy and disappears when the pregnancy is over.
• Spices that can be used for this condition: garlic, cinnamon, fenugreek, ginger, onion, mustard, fennel, saffron,
garcinia, tamarind, coriander, chilli pepper.
Garlic- Oral consumption of the garlic extract have increased the insulin secretion of the rats which were having
diabetic and not in normal rats [167].
4.3. Anti-obesity
Obesity is a common issue, and also it is severe as it directly links with cardiovascular diseases as well as
hypertension, Type 2 diabetes, respiratory disorders and strokes. It is not a disease, and it is metabolic syndrome.
This can occur when the body accumulates fat in massive amounts. Fat accumulation can occur due to undesirable
dietary pattern, lack of physical activities (sedentary behaviour), genetic problems, age, depression, etc. When we
consume fat (triglycerides), it is converted into energy in the body, and the surplus will be stored in different areas
of our body for later usage. Fat is stored in adipose tissue, liver, and muscles as glycogen. This storing mechanism
should take place properly because if not, the blood triglyceride levels will be increased and cause serious heart
diseases. When they are stored in high amounts, it will increase body weight, and that’s the reason for obesity. So,
there should be a balance between fat intake and energy production. The body required energy for many purposes
like brain functions, other internal body functions, physical activities, and etc. So, fat is also an important source
for a healthy life, but managing the level is important to get rid of adverse effects. BMI (Body mass index) is used
to measure the degree of obesity.
Several spices help to burn the excess fat, but it is better if it is combined with the exercises to spend the energy
because sometimes it can increase the blood glucose level when dissolving the stored fat in our body. Considering
about thatis also essential when treating obesity with oral medicine [168, 169].
• Spices that can be used for this condition: turmeric, onion, garcinia
Effects of oxidation:
• Memory loss
• Fatigue
• Muscle/joint pain
• Wrinkles
• Headaches
• Decreasing the eye sight.
Also, oxidation is strongly associated with an ample amount of noncommunicable diseases such as diabetes, anxiety,
cancer, chronic fatigue syndrome, insomnia, fibromyalgia, etc. By increasing the amount of antioxidant usage,
oxidative stress can be reduced as they will block the formation of free radicals [172]. Increasing the consumption
of spices which are having anti-oxidant compounds is a better solution without any harmful effect.
• Spices that can be used for this condition: garcinia.
4.5. Diuretic
Diuretic effects enhance the secretion of urine. The significant advantage is, it reduces the sodium chloride levels
in the body. Diuretics are commonly, used in situations like hypertension, kidney diseases like renal failure, heart
failure, cirrhosis, and nephrotic syndromes (pass considerable amount of protein with urine) [173].
There are synthetic diuretics, and doctors are often prescribing their patients. But the diuretics which are derived
from the natural sources have more beneficial effects for humans. As natural diuretics are more favorable for the
natural body functions, they have minimum side effects and toxicity. Synthetic diuretics and their side effects are
mentioned below [174, 175, 176].
• Loop Diuretics
• Thiazide Diuretics
• Carbonic Anhydrase Inhibitor
• Potassium-Sparing Diuretics
• Osmotic Diuretics
Side effects: Hypokalaemia., Hypomagnesemia, Hypercholesterolemia, Allergic, Hyper glycaemia, Abdominal
discomfort, fever, rashes, nausea, dizziness and muscle cramps, headache, nausea [177].
• Spices that can be used for this condition: cardamom, black pepper, mustard, dill, fennel
40
4.6. Anti-diarrheal
Diarrhea is not a very serious health issue. It is a temporary problem as most of the time it will only last for a few
days. But it can be severe for infants as several child deaths are reporting due to this condition as it causes
dehydration. The main symptoms are watery stools, abdominal pain, fever, bloody stool, etc. The patient can quickly
become dehydrated if this problem lasts for a considerable period. This condition can occur due to different causes,
mainly including bacteria, parasites, viruses, medications, and digestive disorders. Herbal medicines can easily be
used to manage this condition [178]. This anti-diarrheal plant extracts act against microbes like Staphylococcus
aureus, Vibro cholera, Escherichia coli, Salmonella typhi, Shigella dysentery, Shigella flexneri, Shigella sonnei and
etc. which cause abdominal disorders [179].
• Spices that can be used for this condition: cardamom, turmeric, black pepper, dill, tamarind, coriander, chilli
pepper
4.7. Anti-depressant
Depression is a mental health issue as it affects the brain, and that can be found among anyone at any age. It can
even cause physical problems as it directly affects the way someone feels, thinks, and also behaves. Genetic,
environmental, biological, psychological factors can affect human mental health. There are some chemicals in our
brain call neurotransmitters, and when they are not working correctly, this problem can occur as it affects the nerve
connections. Anti-depressants are used to control depression by balancing these chemicals. There are many synthetic
anti-depressant medicines, but they can cause different side effects like nausea, sexual problems, constipation,
dizziness, dry mouth, and etc. So, focusing on natural anti-depressants is a better option, and most of the spices
contain these compounds. Plant extracts aid proper neural communication by binding with the neurotransmitters or
the neuromodulator receptors and altering the neurotransmitter synthesis [180, 181].
• Spices that can be used for this condition: cinnamon, clove, cardamom, nutmeg
41
4.9. Boosting immunity
The immune system is doing an incredible job by fighting against potential factors like microbes and poisons that
can cause harm and make the human body ill. White blood cells, antibodies, complement system, lymphatic system,
spleen, bone marrow, thymus are the main parts of the immune system.
The immune system can be weaker as we get older due to nutrition deficiencies, stress, bad habits, and medical
conditions. When it is malfunctioning, the risk of getting infected and be sick will be increased. So, it is important
to make sure our immune system is functioning properly. Boosting the immune system can be done by incorporating
natural sources like spices.
• Spices that can be used for this condition: garlic, turmeric, clove, black pepper.
4.10. Anti-septic
Words antiseptic and disinfectants most popular among the medical field and other health care settings. So, in those
kinds of places they use chemical agents such as alcohol, iodine, chlorine. Generally, we call them as biocides. They
have been used for hundreds of years. The main targetof using these biocides is destroying microbes effectively
than antibiotics. because most of the time antibiotics have specific intracellular targets while biocide has multiple
targets. In addition to that when antibiotics involve destroying selective microbes at low concentration biocides
destroy or inhibit microorganisms in or on living tissue. The mechanism behind the inhibition of microbes due to
the action of antiseptics is leakage of intracellular constituents inhibition of enzymes, electron transport and
oxidative phosphorylation, but the problem is by using these chemical antiseptic agents it is difficult to eliminate
some microorganisms as well such as Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and
Pseudomonas aeruginosa (P. aeruginosa) due to rapid growth [185, 186, 187].
So, some natural pants have various bioactive compounds which have antiseptic properties. Because they are
recognized as safe than chemicals. Most of the time essential oils of spices are rich in antiseptic properties.
Microorganisms presence in food will lead to various kinds of harmful diseases. Therefore, using spices that have
antiseptic properties inhibit those harm full reflections as well as extend the shelf life of particular food as well. On
the other hand, natural spices help to increase consumer acceptance because it is good for health.
4.11. Anti-clotting
Anti-clotting is done by anti-coagulant agents. Those can be either artificial or natural. So, the thing is doing by an
anticoagulant agent is preventing the formation of blood clots and reduce the thickness of blood. If blood getting
thickerit will, cause for the formation of blood clots and it will lead to serious health problems such as heart attack,
strokes, deep vein thrombosis (blood clots in the leg or lower extremity) and stent thrombosis. Anti-clotting agents
prevent the formation of new blood clots in veins, arteries, heart and the brain of humans. So, nowadays people
move on to artificial medicines due to availability and convenience and for preventing blood clots. But the thing is
those chemical medicines almost have many side effects such as abdominal pain, bleeding, gastrointestinal
dysfunctions, headache, fever, nausea and diarrhea [188]. So, as a substitute, it is better to use spices as a natural
42
product to prevent thrombotic disease which comes from common diseases such as myocardial infarction and stroke.
As well as spices eliminate undesirable side effects and providing additional advantages as well.
• Spices that can be used for this condition: garlic, clove, ginger, chilli pepper
4.12. Anti-inflammatory
Inflammation can be either acute or chronic [4]. Acute inflammation involves to immunity by immune cells and
occurs for a short duration while chronic inflammation occurs due to continuing acute inflammations for a long
time and lead for arthritis, cancers, cardiovascular diseases, diabetes and neurological diseases [189].
So, inflammation is a situation which expects redness, swelling and pain in a particular body part. So, to eliminate
those kinds of things it is better to use natural or artificial anti-inflammatory agent. Most of the time almost all
artificial agent or medicines have considerable side effects, especially to our liver and kidney. Therefore, to prevent
those things better to use natural spices because spices have anti-inflammatory properties.
• Spices that can be used for this condition: turmeric, cinnamon, clove, cardamom, black pepper, ginger,
rosemary, nutmeg, saffron, tamarind, coriander, chilli pepper
4.13. Carminative
The agent which avert or mitigate flatulence (intestinal bloating and gas in the intestinal tracks).So, some of the
spices help to cure those kinds of situation as well as stimulate digestive functions, enhance the secretion of digestive
enzymes to promoting proper digestion.
• Spices that can be used for this condition: cardamom, chilli pepper
4.15. Anti-asthmatic
Asthma is a respiratory disease of lung's airways. Most of the time dust, mould spores, mites, animal hair or feathers
are cause for asthma while cold air affecting too. Asthma is a global disease which thousands of people are suffering.
So, nowadays many kinds of artificial treatments such as nebulization and chemical medicines are available in the
43
world, but there are so many side effects too. Such as dryness or irritation in the throat and mouth, unpleasant taste
in the mouth likewise. Other than that, pills consumption will cause long term diseases as well. Therefore, some
spices have an anti-asthmatic property and it is a good way to cure asthma by using a natural product.
• Spices the can be used for this condition: cardamom, ginger, onion, chilli pepper
4.16. Anti-allergic
Allergy is a situation of the internal body to react to a specific substance by identifying it as a toxic or harmful
compound while it is not. So, these allergen substances can be in a wide range such as dust, food compound, animal
part, medications, pet and pollen likewise. So, these allergens are varied person wise. However, genetics, age, sex,
time and climate can be affecting these allergies. Most of the time allergies are not involved in major problems
while anaphylaxis like allergies needs sudden treatments and medications. In addition to that although artificial
medicines available, they have side effects too as other medicines. Therefore, it is better to use a natural anti-allergic
agent.
4.17. Anti-cataract
A cataract is a common disease which occurs in the human's eye. A dense cloudy like layer will form in the lens of
the eye. Then it is impossible to send a clear picture to the retina through the lens. The picture comes to the retina
as light through the lens. The retina is the place which converts that light into a signal and facilitates to send that
signal to the brain through the optic nerve. So, a cataract will cause for blurry vision, trouble seeing at night, seeing
colours as faded likewise. Smoking, UV radiations, long term steroids and medications and radiation therapies might
cause for cataracts. By doing a surgery it is possible to recover that problem but prevention is better than cure.
4.18. Anti-atherosclerosis
Atherosclerosis is a serious disease which plaque (plaque is a substance which made up from fat, cholesterol,
calcium and other substances that presence in blood.) Deposit in the blood arteries (blood arteries are the type of
blood vessels which carry and circulate oxygen-rich blood all over the body). So, due to deposition of plaque in
blood arteries the arteries getting narrow and blood flow getting slower. Because of that reason heart attacks, stokes
can be expected while increasing the blood pressure also. Finally, these things can be led to even death. Therefore,
better to use spices which have anti-atherosclerosis property. Then it may delay the deposition of plaque in arteries.
• Anti-inflammatory activities.
Turmeric
45
• Boost the immunity system.
• Cure athlete’s foot and nail fungal diseases.
• Clearing the nasal track.
• Prevent lung cancers.
• Stimulate the blood circulation.
• Inhibiting platelet aggregation.
[208, 209, 210, 211]
• Act as antiseptic, diuretic, stomachic, carminative, digestive, tonic
and stimulant agent.
• Has anti-inflammatory activities.
• Use for treatment as bronchitis, renal and vesical calculi, anorexia,
haemorrhoids, dyspepsia, and gastropathy.
• In Ayurvedic medicine cardamom use as a treatment for heart
Cardamom diseases, kidney diseases, urinary diseases, bacterial infections, asthma, oral
infections, food poisoning, tuberculosis, digestive disorders, eye lid
inflammation, bladder diseases, colds, constipation, scorpion and snake
bites.
• Lowering the blood pressure.
• Use against cigarette addiction. [212, 213, 214]
46
• Use for treat to the colon cancers.
• Use as a medicine for arthritis.
• inhibiting cell proliferation and inducing apoptosis and boost anticancer activities.
• Prevent cold, flu and control glucose level. [195, 196, 197, 198, 199]
47
• Enhance urinary activities.
• Improve digestion process.
• Use as treatment for cracked skin, leprosy, swelling, neurological disorders, leukoderma, arthritis,
rheumatoid, wounds, acne vulgaris, eruptions in oral cavity.
• Purging the body of toxins.
• Good for inhibiting cardiovascular diseases.
• Lowering the cholesterol content.
• Use as medicine for haemorrhoids and wounds.
• Good for lowering sugar content.
mustard • Inhibit carcinogenesis and tumerogenesis in colon, breast, skin and lung tissues in animals.[233, 234]
• Good medicine for respiratory diseases such as whooping, cough, bronchitis and asthma.
• Prevent hardening of the arteries.
• Good medicine for dyspepsia, tooth ache and urinary tract infections.
• Kill pathogens in intestinal track and increase he appetite.
• Good substitute for cancer preventive agent.
• Use as a treatment for insect bites, muscle swelling likewise.
thyme • Improve liver functions.
• Use for cure skin issues.[237, 238, 239, 240]
• Use as ingredient of grip water to cure colic pain in babies and flatulence in children.
• Seeds are carminative, diuretic, stimulant, stomachic and galactagogue.
• Contained essential oil of seeds relieves intestinal spasms and griping.
• Chewing seeds cause for fresh breath.
• Cure gas and aids digestion.
• Stimulate milk flow in nursing mothers.
Dill
• Good medicine for urinary issues and mental disorders.
• Increasing appetite
• Use to treat for fever, diarrhoea, vomiting and abdominal distension.
[241, 242, 243, 244, 245]
48
• Stimulating brain functions by improving blood circulations to brain.
• Good medicine for cardiovascular diseases due to increment of blood circulation and stimulation of
heart functions.
• Helps to detoxify the liver and kidney.
Nutmeg • Nutmeg helps to induces sleep.
• Involves to fresh breath and cure dental problems.
• Improve appetite by decreasing stomach disorders.
• Has anti-inflammatory properties and it can be use as pain reliever.
• Cure cold and cough.[246, 247, 248, 249, 250, 251]
• Use as a medicine for inflammatory ailments, bowel complaints and rheumatic pains.
• Facilitate proper digestion.
• Relieve gastric ulcerations and burning sensation.
Garcinia • Use to treat for ulcers, haemorrhoids, diarrhoea, ear infection.
• Exhibit antiaging, anti-diabetic and gastroprotective effects.
• Involves to weight loss by decreasing obesity.[263, 264, 265, 266, 267]
• Good for cardiovascular diseases, vomiting, fever, microbial infections, lactation, pregnancy.
• Exhibit antidiabetic, anthelmintic, anti-inflammatory, analgesic, antivenom
activities.
Tamarind • Help to weight reducing activities.
• Showing Hepatoprotective, anti-diarrheal wound healing activities.[268, 269, 270, 271, 272, 273]
49
• Showing anti-cancer, anti-inflammatory, pain relieving, anti-diabetic, anti-cholesteremic, anti-
diarrheal, anti-lithogenic and anticlotting activities.
• Use as a treatment for microbial diseases.
• Good medicine for asthma.
Chilli pepper • Better for extreme, toothache, wounds, ulcers, arthritis, headaches and night blindness.
• Treating for hypersensitivity.
• Promote and maintain good health.[276, 277, 278, 279, 280, 281, 282]
50
5.1. Insecticidal Effects
Insects have over one million species that live in a wide range of places in this world. On the other hand, insects
being a carrier for parasites and microbes that effect for humans by transmitting various kind of diseases. other than
that, insects are involved in damage, penetrating foods. So due to attracts of insects most of the time total economic
cost getting very high. Therefore, nowadays people use a wide range of synthetic artificial insecticides and due to
toxicity, most of them have been restricted. That is why, the green insecticides becoming prominent which provides
a simple and effective method for insect control. Most of the spices have been used for a hundred years as a source
of bioactive compounds. Spices and their extracts have different insecticidal properties assessing their toxic,
repellent, antifeedant, fumigant, growth regulations. Those spices have either single bioactive compounds or a
mixture of bioactive compounds.
It has been suggested that the disagreeable odor of essential oils or oleoresin can prevent insect attacks.
Eg: Clove is a good mosquito repellent. Eugenol, isoeugenol and methyl eugenol show insecticidal activity to the
storage pathogens such as sitophilus zeamais and tribolium castaneum.
The clove's leaf and bud oils show excellent insecticidal activity against the human head louse(pediculus_capitis)
[283, 284].
Other than that, larvicidal against eggs, larvae and adults of Callosobruchus maculatus [285].
•Ginger and clove containing essential oils were effectively inhibited insects in maize during storage. Eugenol
repellent of maize weevil.
•Black peppercorn exhibits insecticidal properties against moth in clothing.
•Garlic used as a bee repellent and exhibit insecticidal properties against
Delia radicum and Musca domestica
•Bay leaf and basil used as an insect repellent
In order to inhibit insects cause diseases and damages it is better to use spices like green insecticides to save
environmental pollution while improving the quality of human health. In addition to that by using insecticides
contribute for food contamination as well. So, it involves for bring hazardous effects for both environmental and
humans as well. To prevent those harmful things spices and their extracts provide a huge support.
51
Traditional people used some spices to treat for injuries without knowing its scientific background. According to
prior researches, it is revealed that black pepper, clove, cinnamon and turmeric has antimicrobial properties due to
its unique compounds,
• clove -eugenol
• cinnamon -eugenol and cinnamic aldehyde [286, 287]. • black pepper- Piperine [288] • turmeric- curcumin
[289].
Naturally, those spices are in their dry form so it is compulsory to get an extraction by using appropriate solvent.
So, when using a solvent, it is important to concern about the properties of solvents, the purpose of extraction
likewise. because as revealed in previous researches ethanolic extracted extraction have shown higher anti-microbial
properties than other extractions. And also, most of the time those above-mentioned spices have exhibit good
influence against the e-coli bacteria.
And also, active compounds of spices shown their antimicrobial effect by degrading cell wall of microbes,
disrupting cytoplasm membrane and leaking cellular constituents, effects to RNA and DNA likewise.
• Volatile compounds which containing in cardamom helps to inhibit gram negative bacteria [290].
• Possible to inhibit Listeria growth by adding ground rosemary in to pork during its refrigerated storage
[291].
• Essential oils of Cinnamon, bay, thyme, clove and nutmeg effectively inhibit twenty-five general of bacteria
[292].
In addition to that most of foods may susceptible for microbial effects due to its chemical composition and physical
properties. So, due to that case food manufactures using chemical preservatives to extend its shelf life. But the thing
is most of those chemical preservatives have bad side effects. There is a risk of liver cancers if the standard quantity
of nitrite application in to food is exceeded. Therefore, the reason it is better to use natural alternatives to extend the
shelf life of foods.
Spices is one of them it is possible to apply for food to prevent bacterial and fungal infections as well. Generally,
spices have volatile compounds and also those volatiles are recognized as safe [293]. Other than that plant
synthesizes secondary metabolites which have complex molecular structure. So, some of them have antimicrobial
properties. Alkaloids, tannins, cumarin, glycosides, phenolic compounds, terpenes, flavonoids and isoflavonoids are
those secondary metabolites which have antimicrobial properties [294]. And also, the type of spices, the
concentration of spices, the composition of spices, processing condition, storage conditions, microbial content and
species of microbes like factors can be affected for the antimicrobial activity of spices.
Due to the antimicrobial properties of spices Cytoplasm granulation, cytoplasmic membrane rupture and
inactivation and/ or inhibition of intracellular and extracellular enzymes can be expected causes of inhibitory actions
on moulds. Most of spices such as clove, oregano, thyme, cinnamon, and cumin are used as food preservatives.
Spices have secondary metabolites and they are named as antimicrobial agents. Therefore, spices help to develop
new natural antimicrobial agents against the pathogens.
Clove
clove exhibit good antimicrobial activities against oral bacteria [295]. And also, this is generally used in food
industries as a natural flavour enhancer as well as a natural antiseptic agent to increase the shelf life of food by
inhibiting food borne pathogens [296]. Eugenol is the main active component of clove oil and extracts [297]. Clove
inhibiting pathogens by destroying cell walls and membranes of microorganisms and permeate the cytoplasmic
52
membranes either enter to the cells. Then stop the normal DNA and protein synthesis. Eugenol helps to inhibit the
production of amylase and protease enzymes of Bacillus cereus and has the ability to deteriorate the cell wall and
cell lysis.
Oregano
Generally, oregano has applied as a food seasoning and flavouring agent. Carvacrol and thymol are the main
components responsible for the antimicrobial activities in oregano[298]. It is possible to bind with the sterols in the
fungal membranes of C. Albicans strains [299]. Carvacrol could interact with cell membranes by changing the
permeability for small cations[300].So, as the chemical compounds in oregano extract is complex, and it is possible
to inhibit microorganisms through different cell targets.
Thyme
Widely apply as a spice to add a special flavour to foods. In addition to that thyme used to extend the shelf life of
foods due to its antimicrobial activities. The main compound of thyme is thymol. It exhibits its antimicrobial
properties by binding to membrane proteins from hydrophobic and hydrogen bonds and then changing the
membrane’s permeability [301]. On the other hand thymol helps to decrease the intracellular ATP content of E.coli
while increasing the extracellular ATP which can inhibit the functions of plasma membranes [302].
Cinnamon
cinnamon has three major compounds which names are Cinnamaldehyde, cinnamyl acetate and cinnamyl alcohol
[303]. Due to its antimicrobial activities cinnamon use as a health-promoting agent to treat diseases like urinary
infections, inflammation and gastrointestinal disorders [304]. Among them, Cinnamaldehyde helps to stop
biosynthesis of cell walls, membrane functions, and specific enzyme activities as well.
Cumin
Cumin has used as an antiseptic and disinfectant agent in India for a long time [305]. The cumin has major bioactive
compounds such as Cuminaldehyde, cymene and terpenoids [306].
Garlic
Allicin, a diallyl thiosulfinate are the major bioactive compounds present in garlic [307]. Garlic inhibits
microorganisms by penetrating their membranes and destroy its internal organelles then C. albicans like
microorganisms proven to die [308].
Ginger a-pinene, borneoland linalool like volatile compounds contribute for the enhancing antimicrobial activities
of ginger [309].
Fennel
Fennel has significant antifungal and antibacterial activities. It helps to disrupt the permeability of the cell membrane
of S. dysenteriae and result in the leakage of electrolytes, losses of proteins, reducing sugar likewise. Finally, cell
lead for its death [310].
Coriander coriander lead to inhibit all cellular functions of microbes by permeating the membrane [311].
53
Black pepper
It helps to leak electrolytes, ATP, proteins and DNA materials of E.coli by physical and morphological alterations
in the cell walls and membranes [312].
5.2.1.Antibacterial activity
Almost all spices have antibacterial properties. Specially thyme and mint have shown inhibitory actions against
bacteria[313]. Other than that mixture of spices can involve for proper inhibitory actions opposing for various meat
spoilage responsible microbes[314]. In addition to that it is possible to affect bacteria which presence in fish sauce
by using spice mixture which incorporate with cumin, coriander, mustard, black pepper and lemon. On the other
hand, ginger, garlic, mustard and ground clove can be used for retard the growth of E-coli and Salmonella who exist
on mayonnaise[315, 316].
Essential oil of oregano can reduce the log phase of S.typhimuriumwhich presence in the sterilised and natural form
of beef fillets that stored in aerobic and modified atmospheres (An artificially flushed predetermined gases like CO 2,
N2, O2,Ar individually or as a mixture) [317]. Antimicrobial activity of spices can be divided into three groups.
Such as strong, medium and weak likewise. While cinnamon, clove, pimento, thyme, oregano and rosemary had
strong and consistent inhibitory effects against the pathogens[318, 319].
5.2.2Antifungal activity
Cinnamon, clove and oregano can help to inhibit Aspergillus parasitic sand Fusarium moniliforme while clove
contribute for the decreased the aflatoxin synthesis in infected grains[320]. Other than that various concentration of
mint, sage, bay, anise and ground red pepper help to inhibition of Aspergillus parasiticus NRRL 2999 and its
aflatoxin production[321].
Chilli, coriander, pepper, cumin help to inhibit the activities of Rhizopus zygospores, Mucor dimorphosphorous,
Penicillium commune, Fusarium solani like food spoilage responsible microorganisms in addition to that essential
oil of oregano has shown higher inhibitory effect than sorbic acid and effectively retard the activities of
Aspergillus flavus, A. niger, Geotrichumcandidum, Mucor spp., Penicillium roqueforti like
microorganisms[322, 323].
54
5.3. Anti-oxidant
Anti-Oxidant is an any agent that helps decrease the oxidation process which is undesirable in many ways. This
reduction is done by neutralizing the free radical formation. When considering a food, oxidation cause spoiling and
for human, it causes the aging.
Most common oxidation is the lipid oxidation. This process is known as rancidity. It is impossible to completely
stop this process but it can be reduced by incorporating antioxidant compounds with the product. It happens by the
action of oxygen, when fat and oils transformed in to free radicals which are highly reactive and these radicals will
transform into primary oxidative products like peroxides, hydro peroxides [324].
Inside the human body, high concentration of free radicles can be formed due to various negative factors such as
gamma, UV, X-ray radiation, psycho-emotional stress, adverse environmental conditions, smoking, alcoholism and
drug addiction. Those kinds of free radicles are not good for health because it improved the oxidative stress of the
body. And also, when this oxidative stress getting high cells and tissues can be damaged. Because free radicles are
more unstable and reactive substances. Therefore, these conditions can lead for a cancers and heart-related diseases.
Both spices and herbs are very good sources of natural antioxidant. Antioxidant has beneficial effects especially on
food preservation and in the protection of sensory properties. Most of the time in industrially food manufactures are
using BHT, BHA like synthetic antioxidant agents instead of natural things. That kind of synthetic antioxidant agents
may lead to liver damages and carcinogenesis like diseases [325].
There is a large group of bioactive compounds can be expecting from spices which consist of phenolic compounds,
tannins, alkaloids, phenolic diterpenes, vitamins and flavonoids[326, 327].
These compounds perform various kind of antioxidant activities. Eg; flavonoids inhibit the free radicles and form
complexes with catalytic metal ions and inactivate them.
As well as antioxidants can prevent lipids and oils in foods against oxidative degradation. By doing that it helps to
prevent the development of oil and fat rancidity while increasing the nutritional quality and shelf life too. When
considering the lipid oxidation process it is possible to divide it into three groups.
Initiation phase
In this initiation phase, it creates fat-free radicles due to the exposure of an unsaturated hydrocarbon into heat or
light like factors. (most of the time unsaturated fats more prove to oxidation due to its structural double bonds.)
RH H. +R.
Propagation phase
In this stage, peroxide radicals are formed by reacting fat-free radicals with oxygen. then the formed peroxide
radicals react with healthy fat molecules to form hydro peroxide and another fat-free radical. Hydro peroxides are
also readily decomposed by heat, radiation, metals and enzymes to form additional radicals which added to the
chain reaction.
R. +O2 ROO.
ROO. + RH ROOH+R.
55
Termination phase
When two radicals interact with each other termination occurs to form secondary oxidative products. They are non-
reactive products but not good for health.
ROO. + R. ROOR
ROO. + ROO. ROOR +ROOR +O2
R. + R. RR
As mentioned previously many spices act as a primary antioxidant and neutralizing lipid free radicals as well as
terminating free radical chain reaction by donating hydrogen or electron to free radicals by converting those into
stable compounds [328].
Due to these presences of antioxidant, it is possible to inhibit oxidation reaction as well as increase the shelf life of
the food. eugenol, camphene, carvacrol, piperine, myristic acid, myristicin and ascorbic acid are some of the natural
antioxidants which contain in the spices [329].
While the growth of plant it is possible to expose to various stress conditions. So as a response for that kind of
stresses reactive oxygen species (ROS) concentration if getting high. These ROS can harm to the plant tissue.
Therefore, to prevent damages which comes from ROS plants have preventive actions naturally. One is enzymes
activities along with nonenzymatic antioxidants such as ascorbate, glutathione and terpenoids (carotenoids,
tocopherols) which helps to neutralize ROS.
So human bodies also react for the stresses is same as plants. But the thing is most of the time inside the human
body in between the levels of endogenous antioxidant and ROS can be an imbalance. That can be a reason for cell
damage [330]. So, it is important to gain anti-oxidants with the regular meal to prevent cell damages from formed
free radicals and eliminate cancer, cardiac diseases likewise.
There are several factors can be affecting for the antioxidant activity of spices such as the botanical origin of the
spices, place of production, environmental stressors, time of harvest. There are some spices which have active
antioxidant responsible compounds ,
• Turmeric -curcumin
• Red chili- capsaicin
• Clove-eugenol
• Coriander-linalool
• Black pepper- piperine
• Ginger- zingerone
• Cumin- cuminaldehyde
56
These compounds help to prevent lipid oxidation [331]. Based on the literature, an aqueous plus ethanolic extracts
of spices (ginger, garlic, onion, mint leaves, cinnamon, cloves and pepper) exhibit that it is possible to inhibit the
activities of lipoxygenase dependant enzymatic lipid peroxidation. Clove has higher antioxidant activity while onion
showed the least level. (clove > cinnamon > pepper > ginger> mint > onion). In addition to that BHT, BHA like
synthetic antioxidants have higher antioxidant power than natural spices although they are having bad health effects.
And also, when comparing the natural form and extract of spices, extracts have higher antioxidant power than its
natural forms. However, some spices may contain more than one antioxidant responsible compounds. [332, 333]
• Fresh pepper- capsaicin, luteolin, flavonoids- quercetin and ascorbic acids
• Ginger -gingerol and hexahydrocurcumin.
So, these bioactive compounds are responsible for the inhibition of enzymatic lipid peroxidation. When considering
the thermal stability of antioxidant compounds in spices, significant antioxidant power exhibit from the crude
gingerol and garlic which mixed up with groundnut and soybean oil at the 1050C- 1650C [334]. And also, when
spices (turmeric, capsicum and pepper) heated with a souring agent such as tamarind solution there will be
considerable quantitative losses can be expected [335]. Moreover, oleoresin of red pepper (capsaicin) showed
medium antioxidant activity at 1000C and 1500C no antioxidant effect due to the degradation of capsaicin[336]. The
antioxidant activity depending on their chemical composition mainly based on polyphenolic and other biologically
active compounds. Below table listed down the primary antioxidants and the biologically active compounds found
in spices including flavonoids, phenolic acids, essential oils, alkaloids and lignans likewise and those compounds
are identified and quantified by using chromatographic methods (Table 9).
57
Crocin (water soluble carotenoids), safranal, flavonoids, gallic, caffeic, ferulic, syringic,n-
Saffron catechuic salicylic and vanillic acids
There are two main phenolics in spices flavonoids and phenolic acids. Those two contribute to the antioxidant
activities. flavonoids commonly occur as glycosylated derivatives with apigenin and luteolin are generally found in
aromatic herbs such as parsley, rosemary, thyme, quercetin and kaempferol in onion. The antioxidant activities of
phenolic compounds are mainly because of their redox properties (absorbing and neutralizing free radicals)
flavonoids has multiple hydroxyl groups. So, it has higher antioxidant activities against peroxyl radicals than
phenolic acids.
Black pepper.
Prominent spice of the whole world and consider as the king of spices world.
58
There are three major flavour compounds those are, Piperine, s-3-carene, β- caryophyllene likewise. It gives biting,
hot and pungent flavour to the food by acting as a good seasoning agent. Piperine is the main constituent which
responsible for pungency while essential oil brings aroma and flavour. Black pepper has flavouring, preservative
and medicinal properties. Black pepper is better for dishes of meat, eggs and seafood and acting as a multifunctional
spice and contributing the flavour, colour and overall acceptance of the food.
Chili
Capsanthin is the pigment responsible for red colour and contain in all capsicum. Capsaicin is the compound which
gives the pungency. Other than that, dihydro capsaicin gives the unique flavour to the chilli. Chilli uses to enhance
the pungent flavour and colour of foods.
Cardamom
Named as the queen of spices world. there are two types of cardamom such as small and large cardamom. α- terpinyl
acetate, 1-8 cineole, linalool are compounds which help for giving the flavour. Cardamom use for enhancing the
flavour and consumer acceptance of meat, soups and confectionery items.
Cinnamon
Often use as a seasoning agent for meat and fast foods and use for culinary purposes. Use as either ground form or
pieces of bark. Use for culinary purposes preparation of pickle. Cinnamaldehyde, eugenol responsible for the
flavour.
Clove
It contains eugenol and eugenol acetate and it give flavour as well as aroma. Clove is a spice which has strong aroma
and pungency. Both those two involves constructing the spicy taste of clove. Generally, clove use for domestic
culinary uses and in processed meat industry use grounded form of clove. Especially in the processed meat industry
clove use to reduce unpleasant odour and flavour by increasing customer acceptability and palatability.
Coriander
Both leaves and seeds are used and have a mild spicy and sweet flavour. That unique flavour comes from d-linalool,
C10-C14 alkenals which is in coriander. This is an essential component of curry powder. Asians are widely use and
prefer to consume this spice. Popular as pickling spice and seasoning agent of meat products.
Cumin
Cuminaldehyde, p-1,3-mentha-denial are the flavour compounds which gives the characteristic flavour of cumin.
Use to make curry powder and use to make marinates for various kind of meats. Other than that, it helps to enhance
flavour and the overall acceptability of foods.
Turmeric
Use as a colourant and curcumin help to get that unique yellow colour. Use for domestic culinary purposes. Enhance
the appearance of food by acting as a disinfectant agent.
59
Garlic
Allicin is the major component of garlic and it gives the pungent flavour too. It improves the taste and aroma of
food. Mainly use for the preparation of meat and, fish soups. Use as a seasoning agent too.
Ginger
Important spice due to its characteristic aroma and pungency. Gingerol, shogaol, neral, geranial compounds give
the flavour of ginger, increase the organoleptic properties of food and improve the pleasant aroma. Ginger is a major
spice due to its refreshing flavour and aroma and uses for culinary purposes. In addition to that ginger helps to
improve the tenderness of meat due to ginger contained proteolytically active principle named zingibain.
Onion
Commonly used for culinary uses both in industrially and domestically. Unique aroma and pungency give due to
the presence of volatile compounds.
Value addition means the maximum realization of the process of the product through processing, packaging and
marketing. Value addition is essential for the modern competitive world. The original product will be transformed
into a more valuable form by value addition. Consumers pay higher prices willingly for value-added products when
they fulfil their satisfaction. It can be done by farmers, suppliers, processors or distributors [340]. The basic
information related to value added products are given in the Table 10.
60
Black pepper Sterilized black pepper, ground black pepper, cryoground black-pepper powder, pepper
oil and oleoresin, white pepper powder, pepper in brine, frozen green pepper, dehydrated
pepper, green pepper pickle, green pepper sauce, pepper mayonnaise, pepper cookies,
pepper tofu
Ginger Ginger powder, Ginger oil, Ginger oleoresins, Salted ginger, Wines.
Cardamom Seed powder, Cardamom volatile oil, Cardamom oleoresin, Bleached cardamom,
Cardamom tea, coffee, soft drink mix.
Turmeric Turmeric powder, Turmeric oil, Turmeric oleoresin, curcuminoids, Natural pigments.
Chili Chili powder, Paste, Oil, Oleoresin, Dehydrated chili, Canned chili, Brined chili, pickled
chili and fermented chili
Nutmeg and mace Oil, Oleoresins, Powder, Nutmeg butter, Pickle, Candy
After extracting the oils and oleoresins, the spent spices also can be used for other productions by value addition as
they contain a very high amount of nutrients in them especially including dietary fiber, vitamins and minerals (Table
11).
Table 11. Value added products
Spice Composition Product
Spent cumin Dietary fiber 62.1%, zinc 6.5 mg/100 g iron 6.0 protein supplements, [341]
mg/100 g
Spent chili Dietary fiber 44.4%, insoluble dietary fiber 34.9%, Used as an egg yolk coloring agent
soluble dietary fiber of 9.5%, protein by incorporating spent chili in
(19.7%) leghorn layers.[342]
61
Spent ginger Crude fiber 21.9%, Potassium Starch with high gelatinization
0.98ppm, Calcium 0.68ppm, property, Bio ethanol, Production of
(white type) Phosphorus 0.42ppm polyurethane green composites,
[343], [344], [345], [351]
Spent turmeric Dietary fiber (45%), insoluble fiber 43%, soluble Starch, bio films for packaging,
fiber 2% [346], [347]
Spent clove Carbohydrate 51.3%, fat 16.6%, fiber 11.5%, Can be used as a source of fiber,
protein 6.9%, and ash content 6% protein and mineral. [348]
Spent coriander Dietary fiber - 51.7%, Mineralscalcium (4013 Can be incorporated in the flour for
ppm), potassium (4151 ppm), iron (124 ppm), zinc bread preparation [350]
(19.7 ppm), and manganese (34.7 ppm)
• Safety
Pathogenic and toxic microbes can grow in spices which may cause serious health issues. By value addition, they
will be controlled and destroyed.Eg: Salmonella, E.coli, Aspergillus which is responsible for the aflatoxin
production.
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• Convenience
Value addition facilitates easy handling, storing, transportation and most of all it is easier to use a value-added
product rather than a raw product. Due to the packaging, there will be different quantities separately packed, so the
required quantity can be easily collected without any quantitative losses.
• Consumer acceptance
As it increases the continence, appearance and shelf life, it attracts the consumer. Also, when presenting even the
same spice in different valueadded products, people tend to buy them.
• To reduce waste
At some seasons the production of some spices is high. So, there will be a surplus. If this is not managed well it will
be wasted. Value addition helps to preserve and it converts the product into different forms that can be used in other
seasons and also will be able to export.
• Facilitates exports
By enhancing the shelf life, properly packing and by marketing the export market will be opened.
So, spices should also be properly preserved to reduce the losses, consume in off seasons and for the export
purposes.
6.2.1.1. Freezing
Freezing is one of the easiest methods that can be followed to preserve spices. The danger zone is the temperature
range that has the highest microbial activity and it is known as 7.2 °C - 60 °C. This temperature should be avoided
either by increasing or decreasing the temperature. Freezing will reduce the temperature below the lower range of
the danger zone and reduce the microbial growth and activities but it will not destroy microbes. Generally, the
freezing temperature is known as bellow -18 °C [353].
The water activity is the other main factor that should be reduced in order to preserve any kind of food including
spices and herbs as free water is essential for the spoilage reactions and activities like microbial spoilage, enzymatic
spoilage (rancidity, discolouration and etc.).Water activity and moisture content are two different factors and they
are not equal. Water activity is only the free water content and the moisture content includes the bound water content
which is not responsible for any spoilage activity as they are tightly bound to the internal proteins and other
molecules. So, managing the water activity is important. (when the water activity decreased, the moisture content
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will also eventually be decreased as water activity is a part of it). By freezing the free water will be solidified and
thereby the water activity will be reduced. So, it will be preserved to some extent.
The initial investment is high as this method required freezing facilities during the transportation, storing and
marketing steps [354]. Also, choosing a proper packaging material is essential for frozen products. It should be a
good barrier for moisture and vapour evaporation should be easy to seal and should not form cracks at low
temperatures.
• Sun drying
Sun-drying is the oldest and easiest drying method and it is inexpensive as it uses natural sources for the drying
process. But the drawbacks are, there is a high risk of contamination, the conditions cannot be regulated as we want,
time-consuming, high labour cost, only can apply in the day time and cannot apply in every season.
• Microwave drying
Microwave drying is also known as dielectric heating. It is less time and energy-consuming method and it uniformly
dries the raw materials. Normally, 0.3 GHz- 3 GHz frequencies are used for the process. In this method, heat is
generating inside the raw materials so the heating is volumetric (inside to out). Microwave can be combined with
other drying methods like hot air drying, vacuum drying and etc. get a better-quality product [355].
Moisture content is important in this method as the microwave radiations will be absorbed mainly to the water in
the material and these water molecules will move/rotate rapidly due to this energy difference as water molecules
have a positive and negative charge ends and when they try to align with the microwave electrified, they will rotate
fast. A considerable amount of heat will generate due to the friction occurred by the rotation. This heat helps to
evaporate excess water [356].
• Vacuum drying
This method also gives a good quality dried product as it doesn’t require a high -temperature to remove excess
moisture from the raw materials.The temperature and pressure are the most important factors for the drying process.
When the temperature is increased, the drying rate will also be increased. But high levels of temperature will
deteriorate the product by destroying the essential heat-sensitive compounds. So, drying can be obtained at a safe
temperature level by managing the other factor (pressure) [357].
By creating a vacuum, the surrounding pressure will be reduced and the pressure inside the raw material will be
higher than the outer pressure. So, there will be a pressure gradient. This pressure difference pulls out the moisture
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from the high-pressure zone (inside the food) to the low-pressure zone (surrounding). Thereby the excess moisture
will be reduced at ambient or slightly elevated temperatures.
• Freeze drying
Freeze drying also gives a high-quality product with minimal organoleptic changes compared with the other methods
as it uses very low-temperature levels. It dehydrates the product at a very low temperature by the sublimation of
solidified water (solidified water will directly be vaporized without passing the liquid phase). Microbial activities
will be reduced due to the absence of liquid water. This method will not make a huge difference in the primary
structure, shape and the volume of the raw materials as well as the thermally sensitive compounds. But the
disadvantage is, this method is the most expensive among all the dehydrating methods therefore it is not widely in
the food industry [358].
6.2.1.3. Fumigation
Ethylene Oxide is most commonly used for this process. Carbon dioxide is commonly used to dilute Ethylene oxide.
It acts as a fumigant, sterilizing agent, disinfectant and insecticidal agent. It eliminates microbes including yeast,
mould, bacteria (salmonella, E.coli, coliforms) and other undesirable micro-organisms and pests. Laphygma exigua,
Pseudococcidae, Geometridae,Thrips, Acari are some pests which are commonly affecting. [359]. The main benefit
of this method is, it preserves the appearance and flavour of the spice which is very important as it has a very low
impact on them. A small amount of volatile compounds can be lost as this method uses a low pressure in order to
remove ethylene oxide [360].
The major disadvantage is ethylene oxide can form Ethylene chlorohydrin and ethylene bromohydrin by reacting
with the compounds in spices. These forming products are not safe and specially chlorohydrin is known as a
carcinogen. So, some countries like Japan, European countries have banned the usage of ethylene oxide.Apart from
ethylene oxide, Methyl bromide (32/m3 for 24-48 hours) and aluminum phosphide (3-6 g/m3 for 7 days) are also
used for this purpose.
6.2.1.4. Irradiation
This method is also used for the decontamination purpose. Gamma rays (most common), Electron beam and X-rays
are effectively using for this method. Should not exceed the recommended dose of 3-10 kGy as safety issues may
occur. This method is energy efficient, effective [361]. It allows decontaminating dried aromatic spices, herbs and
other vegetable seasonings as well. To avoid re-contamination products to be treated should pre-packed. However,
there is an issue of the consumer acceptance of irradiated products.
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6.2.2. Polishing
Polishing is done to improve the outer appearance of dried spices like ginger and turmeric by removing the wrinkles
on the surface. Traditionally the dried spices were rubbed on a hard surface and now there are power operated
polishers for the purpose.
6.2.3. Milling
Milling means reducing the particle size of the raw material. Spices are different plant parts including barks,
rhizomes, seeds, roots etc. Spices can be incorporated as it is but they have a small surface area. The compounds in
spices are responsible for the nutritional, medicinal, sensory, preservation properties. So, milling process is essential
to facilitate incorporation of those compounds with another product well and to enhance its texture.
Milling is done by breaking the internal bonds between particles and it required a force. Shear, compression and
impact forces are used individually or as a combination for this purpose.
In traditional milling, there is a major disadvantage due to heat generation. This heat generation mainly occurs due
to friction. High levels of temperature are not suitable as it destroys the thermally sensitive compounds in spices
and thereby affect the colour, aroma, taste like sensory properties as well as the medicinal and nutritional properties
in spices. Normally, it increases the temperature up to 43-95 °C [362].
Shifting towards cryogenic milling is a great solution for this. This method uses refrigerants like liquid nitrogen at
-196.6 °C,Carbon dioxide (CO2), Dichlorodifluoromethane (freon-12), Xenon krypton, Neon which are having a
very low temperature to reduce the temperature of the raw materials. By reducing the temperature they will be able
to absorb more heat without making any harm to the thermally sensitive compounds [363].
Eg: When the temperature is reduced down to -30 °C, and the heat generation due to the friction may cause rising
of the temperature in 30 °C, still the raw material will have 0°C. So, this temperature is safe for the thermally
sensitive compounds and they will be preserved. So, by that, the moisture losses, volatile oil losses, damages to the
chemical composition, oxidation will be reduced.
Apart from that there are many more other advantages of the cryomilling over the traditional milling such as, milling
efficiency and production rate is high (Due to Fast freezing cause internal cracks due to the stress and spices with
high amount of fats will be solidified and avoid melting and sticking on the surface), Low energy consumption,
gives finer particles and Suitable for materials which are more prone to ignite [364].
Grounded spice products are more convenient to use but they are more susceptible for the spoilage, oxidation,
flavor/colour/aroma losses. So, proper handling, packaging and other preservation techniques should be followed
after milling.
Eg: Chili powder, turmeric powder, pepper powder, cumin powder, coriander powder, Culinary powders such as
rasam powder, Masala blends such as Garama masala and Meat masala, Seasonings like green chili masala, noodles
tastemakers, mint powder and etc.
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6.2.4. Extraction of oil and oleoresins
Most popular oil and oleoresin extraction methods are steam distillation, Solvent extraction/ Hydrocarbon
extraction, Super critical carbon dioxide extraction and Enzymatic treatments.
Essential oil
Essential oils are responsible for the aromatic characteristics of a spice and they are a mixture of volatile compounds.
Essential oils are 75-100 times concentrated with the flavor producing compounds than in raw spices (Table 12).
Clove
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Garlic 0.1–0.4% [375]
Fennel 6% [376]
Oleoresin
Oleoresin is made of oil and resins. It can be in liquid, semi-solid or solid form. They are extracted by the solvent
extraction method. An oleoresin contains different compounds including essential oil, pigments, fixed oils, natural
anti-oxidants, pungent constituents and etc.
The quality of the oleoresins is based on raw material quality. Oleoresins are mainly used for flavoring and many
of them contain natural anti-oxidants. But it can be hard to handle as it is very concentrated. Also, the flavour profile
depends on the solvent used for the extraction.
Oleoresins can be extracted by grounded fresh spices or by the residual part after the essential oil extraction. The
strength of the flavor and the yield of the oleoresin extracted can be varied with the cultivars, maturity and quality
of the raw material, chosen solvent (alcohol/ acetone/ ethylene dichloride) and the method of extraction.
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o Raw material selection o Milling and obtaining suitable size particles o Solvent
The important parameters of the Oleoresins are given in the Table 13.
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Cardam om Dark brown oleoresin is a antibacterial and [381]
yellow liquid antifungal properties.
with a sweet spicy, warming
fragrance. Treatment for fever,
digestive and urinary
disorders.
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Nutmeg Yellow- reddish brown viscous coloring and [383]
18 to liquid which has spicy odor flavoring agent in the food
26% industry
(extracte d
with use as a treatment for kidney
and digestive problems.
ethanol)
31–37%
( nzene)
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6.2.4.4. Microwave-assisted extraction method (MAE)
MAE is a rapid, simple extraction method which is having benefits over the solvent extraction method. For this
method, a solvent and electromagnetic radiations with frequencies between 300 MHz- 300 GHz are used. To extract
polar compounds hexane is used and for non-polar compounds, methanol or methanol mixed with water is used.
During this process, the cell membranes of the plant cell will be swelled and ruptured due to the microwave effect
as moisture contains in the plant material absorbs the energy and evaporates. This will increase the pressure on the
cell wall and rupture the cell as membrane and release the constituents to the solvent [394]. Additionally, this method
required a filtration or centrifugation step to separate the residuals. There are both open and closed vessels for the
MAE method. Close vessels are used to extract the compounds at high temperatures and pressure and open vessel
are used for the extraction at ambient conditions [395, 396].
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6.3. Packaging
Packaging plays a very important role in preserving the product as well as to maintain the quality and safety of the
product. If the product is not packed properly the composition of the compounds which are responsible for the
aromatic and flavor properties can be changed undesirably and that will be a serious issue, especially for spices and
herbs.
Importance of packaging:
• Avoid oxidation and other unfavorable reactions • Act as a barrier to the external deteriorating factors
• Avoid microbial spoilage.
• Protects from insects and rodents.
• Enhance the convenience
• Provide a uniform measure of the contents.
• Acts as a communication medium by displaying the brand, labeling, nutrition special features and standards.
• Promote and influence to by the product
Mainly can happen by evaporating the volatile compounds due to the high temperature.
o Colour bleaching
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This happens specially in spices which contain natural pigments like paprika, turmeric, saffron, red chilies
and etc. Colour loss occurs mainly due to the oxidation and it is directly accelerated by environment factors
like light, temperature and humidity.
o Moisture absorption
Specially powders like free-flowing materials will be soggy and also caking can be observed if the packaging
material is not a proper moisture barrier. Also, this moisture absorption will accelerate the oxidation,
microbial spoilage and insect attacks.
o Microbial storage
Can occur when the moisture content is high, temperature is maintaining at the danger zone (specially the
room temperature) and also due to the non-hygienic practices.
Requirements of a packaging material for spices o Should have an enough strength to hold
o Should protect the contents from microbes and insects o Should be a good barrier for environmental factors
like temperature, light, humidity and oxygen.
o Should be a good aroma and flavor barrier to avoid aroma losses and any gains of undesirable foreign odors
o Should be a good oil/fat resistance as most of the spices contain high amount of oil.
o Should not react with the product or mix with the product o Should be attractive and convenient to handle
o Should be economical, available o Better if the packaging material is environment friendly, reusable and
recyclable.
• Bulk packaging:
Whole spices will have a lower risk of oxidation, loss of volatile compounds and sensory properties as the surface
area is lower when compared with other disintegrated spices. The major consideration is preventing the penetration
of insects and pests. Other than that, it is better if the packaging is also a good barrier to moisture to avoid any
moisture uptakes and infections. Polythene lined bags have the ability to fulfil this requirement.
Packages- Gunny bags, A twill bags, B twill bags, Multiwall paper sacks, cotton bags, Polythene lined jute bags,
HDPE/PP woven sacks
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• Unit packaging:
Even though the whole spices are mostly bought in loose, mostly in cities packed whole spices is a requirement.
Especially people used to buy branded spices and they are identified by the package. Spices are commonly packed
in 50g – 1kg units. Consumer preference is considered when choosing packaging materials.
Eg: Polyolefin pouches, PET/PE laminated packages, Aluminium foil laminated packages (Good barrier for light),
Glass bottles
Spice powders
Powdered spices should be properly packed because they have lost their natural protective barriers like cell
membranes, cell walls due to the milling process. So, the compounds which are responsible for flavour and aroma
like sensory properties can be easily lost and also due to the high surface area, these products are more prone to
oxidation, moisture pickups and other undesirable reactions. Also, they can be easily spilt out.
• Bulk packages
Most commonly bulk spice powders are required by the industries and also for wholesales.
Packages: Multiwall paper bags, Glass containers, Metal containers, lined textile sacks.
• Unit packages
Packages: PET containers, PET-G co polymer containers, Glass containers, metal containers, polyolefin pouches,
Al foil laminated pouches, Metalized polyester pouches.
It is found that Aluminium foil laminated, double pouch of glassine and LDPE packages provided good protection
for turmeric powder as there were no significant changes in the quality for six months.
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