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LBPH Hematology SOP 003

SOP HEMA

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65 views3 pages

LBPH Hematology SOP 003

SOP HEMA

Uploaded by

kayentampus6
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Page No.

1
COAGULATION TESTS SPECIMEN HANDLING
Revision No. 0

LBPH-Hematology-SOP-003 Standard Operating Effectivity.


Procedure
1.0 PURPOSE

This Standard Operating Procedure (SOP) provides guidelines for proper specimen collection,
handling, and processing of coagulation tests. These tests are essential for evaluating blood clotting
disorders and monitoring anticoagulation therapy.

2.0 SCOPE AND APPLICATION

This SOP covers the specimen requirements, collection procedures, and handling guidelines for
coagulation testing in the Hematology section of the Le Bailer Clinical Laboratory. It applies to all
preanalytical processes for coagulation tests including Prothrombin Time (PT), Partial Thromboplastin
Time (PTT), and other related coagulation assays.

3.0 REFERENCES

Turgeon, M. L. (2012). Clinical Hematology: Theory and Procedures. Lippincott Williams & Wilkins.

Brihi, J. E., & Pathak, S. (2024, June 8). Normal and abnormal complete blood count with differential.
StatPearls - NCBI Bookshelf.

Lynch, E. C. (1990). Peripheral blood smear. Clinical Methods - NCBI Bookshelf.

Blood smear. (n.d.). Mount Sinai Health System.

4.0 DEFINITION OF TERMS

Citrate a diagnostic medium used to differentiate bacteria based on


their citrate utilisation ability, helping identify specific bacterial
species.

Blue-top tube a type of blood collection tube that contains sodium citrate as an
anticoagulant, commonly used for coagulation studies and tests
such as prothrombin time (PT) and activated partial
thromboplastin time (aPTT).

PT measures the time it takes for blood to clot, evaluating the


extrinsic pathway of coagulation; used to monitor anticoagulant
therapy effectiveness.

PTT/APTT PTT (Partial Thromboplastin Time), also known as APTT


(Activated Partial Thromboplastin Time), measures the intrinsic
pathway of coagulation, assessing the blood's clotting ability
and monitoring anticoagulant therapy.

5.0 RESPONSIBILITIES

238
Page No. 1
COAGULATION TESTS SPECIMEN HANDLING
Revision No. 0

LBPH-Hematology-SOP-003 Standard Operating Effectivity.


Procedure

Medical Technologist/MT/Lab Performs specimen processing and collection, documents receipt


Technician and rejection, documents QC results, and reports results

Pathologist Reviews and approves procedures. Consults on unusual findings

6.0 REQUIREMENTS

6.1. Materials and Equipment


Equipment
● Centrifuge
Glasswares
● Blue-top citrate tubes (3.2% sodium citrate)
Miscellaneous
● Transfer pipettes
● Plastic secondary tubes
● Labels/markers
● Ice packs/cold storage (if needed)

7.0 PROCEDURE

7.1. Specimen Requirements


7.1.1. Acceptable specimens:

I. Whole blood collected in 3.2% sodium citrate (blue-top) tubes


II. Proper blood-to-anticoagulant ratio (9:1)
III. Specimens processed within 4 hours of collection
IV. Specimens properly labeled with patient identification

7.1.2. Unacceptable specimens: —- Reject and Recollect

I. Clotted samples
II. Grossly hemolyzed samples
III. Samples drawn above IV
IV. Samples with fibrin strands
V. Samples with platelet clumps
VI. Specimens older than 4 hours
VII. Specimens exposed to extreme temperatures
VIII. Underfilled/overfilled tubes
IX. Improper blood-to-anticoagulant ratio
7.2. Slide Preparation

7.2.1. Verify patient identification.


7.2.2. Fill tube to indicated mark ensuring proper blood-to-citrate ratio
7.2.3. Mix gently by inverting 3-4 times
7.2.4. Label specimen immediately
7.2.5. Transport at room temperature

239
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COAGULATION TESTS SPECIMEN HANDLING
Revision No. 0

LBPH-Hematology-SOP-003 Standard Operating Effectivity.


Procedure
7.3. Processing

7.2.1. Centrifuge specimens at 1500-2500g for 15 minutes


7.2.2. Check for platelet-poor plasma (<10,000 platelets/µL)
7.2.3. Transfer plasma to plastic secondary tube if testing is delayed
7.2.4. Maintain at room temperature if testing within 4 hours
7.2.5. Freeze at -20°C if longer storage is needed

7.3. Proceed to testing procedures using manual method or automated methods (CA-600
Sysmex).

8.0 PRECAUTIONS

8.1. All patient specimens should be considered potentially infectious and must be handled with
precautions used for human blood, as described in CDC recommendations and in compliance with the
Federal OSHA Bloodborne Pathogen Standard, 29 CFR part 1910.1030.
8.2. Verify proper tube fill volume before processing
8.3. Avoid sample contamination during processing
8.4. Monitor centrifugation speed and time
8.5. Maintain proper storage conditions

9.0 LIMITATIONS AND INTERFERENCES

9.1. Hemolysis can affect results


9.2. Incorrect blood-to-citrate ratio invalidates results
9.3. Delayed processing may affect results
9.4. Exposure to extreme temperatures can compromise specimen integrity
9.5. Patient has a recent intake of any form of blood thinners and anticoagulants.

10.0 APPROVAL
Prepared: Reviewed:

Angelie Leonado, RMT Nice Jane Noynay, RMT


CHIEF MEDICAL TECHNOLOGIST

Approved:

Kayen Tampus, RMT, MD


PATHOLOGIST

240

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