CASA System

COMPUTER ASSISTED
SEMEN ANALYSIS
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CASA System

Overview

A combination of hardware and software

components to calculate semen analysis
parameters (count, motility, and morphology)
automatically

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CASA System

Features

Huge database with backup and restore commands for case studies
includes case data, images, videos, and reports with searching tool

Full customized header and footer for report

Offline calculation

Report includes sperms images show the motion path and also show
the head with akrosome for morphology results

Full automatic motility calculations “one click” with the ability to edit
results by adding or removing motile and immotile sperms

Full automatic morphology calculations for sperm head “one click”
with the ability to edit sperms dimensions and add or remove sperms

Outputs can be burned to a CD includes morphology images, motility
videos, and PDF report

Simple and Easy
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Benefits

The case studies saved, organized, retrieved and reviewed

easily

Parameters follow-up for case studies

The medical lab can design report header & footer, and also
can print the report on a printed paper

The medical lab can record case studies to be calculated later

The printed images and the numeric results will ensure each
other

Flexibility, accuracy, and fastness

Non-computer specialist can use the product easily

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Specifications

 Hardware:
Microscope - Camera - PC windows XP or later - Color printer
 Software system can
1- Connect and receive video stream
2- Record videos and capture images
3- Calculate count, and motility parameters
4- Calculate morphology parameters
5- Prepare outputs (printed report and CD)
6- Make a database for cases with videos, images, and
report

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User Manual

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CASA System

Semen sample preparation

To get the most efficiency should consider the following when preparing a sample for
semen analysis

• Ensure well cleaning of slide & cover

• Well semen sample homogenate, this will help the system in concentration calculation
• Use a heavy cover to have one field under the microscope, this will help in motility parameters
• Use a suitable dyed sample for morphology (the akrosome area will be lighter in color than
the rest of the sperm head)
Motility sample Morphology sample

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Connecting
Run the CASA system installation CD and follow the instructions
Connect the camera to the microscope
Connect the camera to the PC either through a USB connector or standard RC
video to a video acquisition card

USB Camera RC Camera

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CASA System

System Calibration

 You will need a good semen sample and a calibrated slide to proceed in the
calibration process, you need this process only the first time you run the system
Motility sample Morphology sample Calibrated slide

 From “Tools” menu select the item

“Video Settings” to start calibration

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System Calibration

From the right side you can adjust the video signal settings
to have the best quality
Put the calibrated slide then press the button
“Capture Motility Calibrated Slide”
Put the semen motility sample then press the button
“Capture Motility Sample”
Put the semen morphology sample then press the button
“Capture Morphology Sample”
Now press the button “Save Video Stream Settings” to save
your work
 From “Tools” menu select the item
“Calibration Settings” to complete the calibration
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System Calibration
Here you can complete the calibration process for the calibrated slide captured in the
previous step. Use the button “Draw ruler of the box side” above the calibrated
slide image to make a ruler represents the box side

Type the actual box side length (mm) in the text box on the
top right corner of the window then press the button
“Calculate Factor” below the text box to calculate the
calibration factor

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System Calibration
Here you can complete the calibration process for the morphology sample captured in
the previous step. Use the button “Scan for sperms” on the top right corner of the
window to detect the sperms, you can use the 3 button above the sample image to
enhance the sperm boundaries

Select the normal sperm by clicking on it then type the

error factor, after that press the button
“Set the normal sperm” to finish your work

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System Calibration
Here you can complete the calibration process for the motility sample captured in the
previous step. Use the button “Scan for sperms” on the top right corner of the
window to detect the sperms

Select the smallest sperm by clicking on it, after that

press the button “Set the smallest sperm” to finish
your work

Calibration is done

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System Settings
– The settings window has 5 pages to control the following
Saved directories, templates, printing, normal ranges, and login information
From start menu select “Settings & Options” to open the settings window

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CASA System

System Settings
The first page adjust the settings of the destination folders to save data (images &
videos), and make backup

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CASA System

System Settings
The second page manages the templates to be used in none numeric parameters

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CASA System

System Settings
The third page manages the report & printing settings in the first page you can set the paper
style, and your header and footer design
Empty white paper, this option will use your predefined header and footer in printing
Printed paper, this option will print the parameters only since you have a paper with header
and footer
For the option empty paper you
can determine
The header style either a single
image or a logo & a text
The footer style either a single
image or a text

The next page in this page will

help in header & footer
design
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CASA System

System Settings
You can design your own header & footer here
Select the header style (Single Image OR Logo & Text), then you can select the image and type
a full formatted text
Also for footer select the style (Single Image OR Text), then you can select the image and type
a full formatted text

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System Settings
The fourth page manages the normal values of the numeric parameters

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System Settings
The fifth page manages the login information (user name & password)

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Adding Cases
From “File” menu select “New Case” to open the cases window
This window manages cases data
The left side is a searching tool searches for cases by code, name,
or reference, and shows the search results in a list.
The right side is the case information to be updated or added

The button “New Study” on the

bottom right corner will open
the video signal window to add
a study to the selected case

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Recording Study
This window records videos & images to be used in calculations

Put the motility sample then record

videos for motility calculations
Put the morphology sample then capture
images for morphology calculations
Set the volume of the sample in the
volume text box, and the dilution
percentage if exists

The button “Calculate” will open the

case study window to calculate the
study

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History
From “File” menu select “New Study” to open history window
The left side is a searching tool for studies by code, or name, and shows the results in a tree view
(each root represents a case, and the root children represent the studies of the root case
The right side is the important parameters results of the selected study

The button “New Study” opens the video signal window to add a study to the selected case
The button “Add files” opens the video signal window to add videos and images to the selected study
The button “Open study” opens the study window
The button “Make CD” makes a CD
for the selected study includes
videos, images, and report
The button “Report” opens a preview
of the selected study report if
exists

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Study Details
The study window shows the recorded video & images to be calculated, and the report fields. The left
side of the window shows 2 lists one for videos the other for images, click on a file to view
The images are used in
morphology
calculation, just click on
the button calculate to
start automatic
calculations of
morphology parameters
The button “Add to Rep.”
adds the selected image
to the report
The button “Edit Field”
switches you to the
mode of manual editing

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Study Details
Select a video file to show the video controls. The button “Play” will play the video file, the scroll bar
represents the frames of the selected video
The videos are used in
motility calculation,
just click on the button
calculate to start
automatic calculations
of motility parameters
The button “Add to Rep.”
adds the selected video
to the report
The button “Edit Field”
switches you to the
mode of manual editing

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Study Details
After the automatic calculations
Each sperm in the images will have a rectangle around the head (green color for normal sperms and
red color for abnormal sperms)
Each sperm in the videos will have a rectangle around the head and a line shows the motion path
Green = Class A sperm, Blue = Class B sperm, Yellow = Class C sperm, and
Red = Class D sperm (immotile sperm)

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Editing mode of Images

In this window you can edit the sperms. Click on a sperm then you can set or edit the morphology
details from the check list on the left
Also you can use the button
“Remove sperm” to remove
any bodies you decided that
they are not sperms
The button “Add sperm” enables
you to make a polygon point
by point represents a new
sperm head. After finishing
adding the polygon a popup
will appear to make another
polygon for the akrosome
The button “Save” saves changes
The button “Cancel” discard
changes
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Editing mode of videos

In this window you can edit the sperms. Click on a sperm then you can change the velocities and
class from the inputs on the left, then press “Apply changes”. You can use the button “Remove
sperm” to remove any bodies you decided that they are not sperms

The button “Add immotile sperm”

enables you to make a rectangle
around a new immotile sperm
head
The button “Add motile sperm”
enables you to make a rectangle
around a new motile sperm
head, then you will be asked to
draw a line shows the new
sperm motion path
The button “Save” saves changes
The button “Cancel” discard
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Report preparing
After calculation and editing the automatic calculated parameters results will appear in this page
You can complete none automatic calculated parameters
Also you can edit the automatic calculated parameters, the effect of editing them will reflect the
related parameters automatically

The numeric parameters will have a

green color if their results are in
the normal range, and a red
color if not.
You can preview the report by
pressing the button “Preview”
or send the report directly to the
printer by pressing the button
“Print”

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Report Page 1
Includes the important
parameters and a
comment

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Report Page 2
Includes the motility
parameters in details.
Classifications, velocities,
and motility images
showing each sperm
path

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Report Page 3
Includes the morphology
parameters in details.
The occurrence of each
morphology type, and
morphology images

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Statistics
From “File” menu select “Statistics” to open statistics window
The left side is a searching tool for cases by code, or name, and date interval
Select a case to arrange the studies results in the top right list, and draw a chart represents the
variation of each parameter along the studies

You can select one or more

parameter to print the
values and the chart

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Backup & Restore

The “Tools” menu has 2 items for backup and restore processes

The backup & Restore process works on the calibration, settings, and cases studies data not
the images & video files

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