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Bio F110

The document is a course handout for the Biology Laboratory course (BIO F110) at Birla Institute of Technology and Science, Pilani for the second semester of 2024-2025. It outlines the course description, objectives, experiment plans, evaluation scheme, and make-up policy. The course aims to provide hands-on experience in fundamental biology concepts and techniques through various experiments.

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25 views2 pages

Bio F110

The document is a course handout for the Biology Laboratory course (BIO F110) at Birla Institute of Technology and Science, Pilani for the second semester of 2024-2025. It outlines the course description, objectives, experiment plans, evaluation scheme, and make-up policy. The course aims to provide hands-on experience in fundamental biology concepts and techniques through various experiments.

Uploaded by

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Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani

Pilani Campus
AUGS/AGSR Division

Second Semester 2024-2025 Date: 5.1.2025


COURSE HANDOUT (PART-II)
In addition to part-I (General Handout for all courses appended to the timetable) this portion gives further
specific details regarding the course.
Course No. : BIO F110
Course Title : BIOLOGY LABORATORY
Instructor-in-charge : VISHAL SAXENA
Team of Instructors :Sudeshna Mukherjee, Syamantak Majumder, Uma S Dubey, Soumitra Ghosh,
Mahima Sharma, Shristy Shreya, Satya Omprabha, Lisa Kabiraj, Ritobrata Bhattacharyya, Juhi Kumari,
Hariharan J, Lourdes Jennifer S, Ananya Bhatt, Sushama Biswas, Sulagno Chakraborty, Subhojit Roy,
Swathi K Sorab, Neha Kanwar Nirwan, Ritu Jangir, Himadri Sharma, Deepti Kaushik

Course Description:
Understanding Biological sciences, analysis and estimation of biomolecules, preparation of temporary
slides for microscopic analysis, study of cell structure and division, investigation of catalytic activity of
enzyme, physiology of plant and animal systems, diversity of living systems.

Scope and Objective of the course:


The major objective of this course is to offer a hands-on experience on fundamental aspects of practical
biology. The student would observe and understand various biological phenomena and also be equipped
with some simple techniques which form the basis of research in biology. The course has been revised to
run parallel to General Biology classes.

Text Book/Manual: Laboratory Manual for Biology, BITS Pilani Semester II 2024-25 (Revised edition)
Reference Book: Simon, E.J. et al: Campbell Essential Biology with Physiology (5th Edition,
BITS Pilani custom edition). Noida: Perason India Education Services Pvt. Ltd., 2015
Experiment Plan

Experiment No. Name of the Experiment Learning Outcome


Experiment – 1: Protein Lab: Measurement of total protein Proteins: the building blocks; their role in
content in the given sample by Lowry’s humans; methods to quantify proteins in
method. different samples.
Experiment – 2: Enzyme Lab: To study the effect of the Properties of an enzyme and chromogenic
enzyme lactase on milk detection methods.
Experiment – 3: Transport Lab: To study the phenomenon Effect of tonicity of a solution on different
of plasmolysis in onion peel. cell types; osmosis & osmoregulation.
Experiment – 4: Stomata & Transpiration Lab: Visualizing stomata and appreciating its
Preparation of temporary mount of leaf role in gasous exchange in plants.
epidermis to study the structure of stomata Understanding the role of stomata in
and Measurement of transpiration rate controlling transpiration in plants.
using Cobalt chloride method Transpiration rate and factors affecting it.

Please Do Not Print Unless Necessary


BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
AUGS/AGSR Division

Experiment – 5: DNA Lab: To extract DNA from banana Basic knowledge about the genetic
and observe DNA bands in an agarose gel material, principle of its isolation.
Visualization of DNA bands on an agarose
gel.
Experiment – 6: Physiology Lab: Measurement of Blood group incompatibility, genetics
haemoglobin content in the human blood behind blood group inheritance,
and determination of blood group and Rh hemoglobin and its importance; Blood
status. typing.
Experiment – 7: Bioinformatics Lab: To understand Understanding the application of
Sequence-Structure-Function relationship Bioinformatics in sequence comparision
of protein through bioinformatic analysis and anlysis of various macromolecules like
protein, RNA and DNA.
Experiment – 8: Chromatography Lab: Separation of Basic knowledge about chromatography,
chlorophyll pigments by paper Rf value, partition coefficient, separation
chromatography of compounds from a mixture
Experiment – 9: Microscopy Lab: Observing characterstic Learning to handle and use microscope,
features of important specimen making observations using different slides.
Permanent/ temporary mounts to
understand the relationship between
structure and function.
Note: The order/number of experiments listed above may change depending on the availability of chemicals,
enzymes, and other requirements for a specific experiment.

Evaluation Scheme: Evaluations will be based on laboratory experiments and the related materials covered in
Gen Bio classes.
S. Evaluation component Duration Date, time and Weightage Nature of
No. Venue (%) component
1. Day to day Evaluation (Class Participation 1h 50min Daily Lab 48% OB
+ Performance + Record)
2. Viva – Voce - Mid Term & End 20% CB
term
3. Comprehensive Exam 1 hr. 32% CB
Notices: Notices will be displayed on Nalanda.

Make up Policy:

No make ups will be granted for daily labs. Make up is only allowed for genuine cases with prior
permission/discussion with the instructor-in charge. Genuine cases such as hospitalization, (upon production
of the relevant documents as records/ proof) will be considered upon discussion with team.

Instructor-in-Charge
BIO F110

Please Do Not Print Unless Necessary

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