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BIOL200L General Biology I LAB

This document outlines the syllabus for a General Biology I Laboratory course. It provides information on the instructor, grade distribution, course description, prerequisites, textbook, attendance policy, and a peer review policy. The course consists of 12 topics that will be covered in weekly 2-hour laboratory sessions over the semester. Assessment includes practical exams, reports, a midterm exam, and a final exam. Topics include microscopy, organic compounds, diffusion and osmosis, photosynthesis, enzyme activity, cell division, electrophoresis, and hamster dissection.

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137 views4 pages

BIOL200L General Biology I LAB

This document outlines the syllabus for a General Biology I Laboratory course. It provides information on the instructor, grade distribution, course description, prerequisites, textbook, attendance policy, and a peer review policy. The course consists of 12 topics that will be covered in weekly 2-hour laboratory sessions over the semester. Assessment includes practical exams, reports, a midterm exam, and a final exam. Topics include microscopy, organic compounds, diffusion and osmosis, photosynthesis, enzyme activity, cell division, electrophoresis, and hamster dissection.

Uploaded by

adeeb ahmed
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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LEBANESE INTERNATIONAL UNIVERSITY

BEIRUT CAMPUS

BIOL 200L: General Biology I Laboratory (1.0 credit)

Course Syllabus
Spring 2009-2010

INSTRUCTOR: Mrs. Maya Hallak


OFFICE HOURS: Monday 8:00-10:00
Friday 12:00-1:00

GRADE DISTRIBUTION – LABORATORY


Practical Exam and Evaluation 10%
Midterm 30%
Laboratory Reports 20%
Final Exam 40%

COURSE DESCRIPTION
General Biology course introduces the student to the organization of living systems, energy transfer, continuity of
life, biodiversity, and classification of living things. The topics include: biological history; structure and functions of
cells and cellular organelles; cell division; general biochemistry; cellular respiration; photosynthesis; DNA structure
and function; protein synthesis; heredity; evolution; animal development; classification; and introductions to viruses,
prokaryotes, Protista, and Fungi. This course laboratory component is application of the above-mentioned themes.

General Biology lab provides hands-on experience of some of the concepts discussed in the latter course

COURSE PREREQUISITE
BIOL 150, FRESHMAN BIOLOGY II; BIOL 200, GENERAL BIOLOGY I (CO-REQUISITE) SOPHOMORE
STANDING

REQUIRED TEXTBOOK
Laboratory manual as distributed by the department/ Laboratory notes and handouts.
REFERENCE BOOK
Life on Earth; 4th Edition (2006) – Geralad Audesirk, Teresa Audesirk and Bruce Byers. Prentice Hall, USA
(www.prenhall.com)
ATTENDANCE
For legitimate reasons only, a student is allowed to absent him/herself for a maximum of 2 lab sessions. Absences
beyond the specified limit will be assessed by the Chairman of the Department and will result in an AW or other
disciplinary measures. An unexcused absence from a drop quiz will result in a zero on that drop quiz. An unexcused
absence from an exam will result in a zero on that exam.

UNIVERSITY PEER REVIEW POLICY

This is a new policy at LIU in which during the semester, all classes will be visited by
instructors either from the same or different departments. This policy helps instructors
figure out ways to improve student educational experiences in class and eventually their
performance.
LABORATORY COURSE OUTLINE BY TOPIC

Topics Title / Chapter Assignment - Based on the Laboratory Manual # Hours

1 Microscopy Revisited (Laboratory Experiment 1) 2

 Light microscope
 The compound microscope(explanation)
 Use of the microscope (preparation of “e” slide)
 The advantage of staining and wet mounting
 The stereomicroscope (explanation)
 The electron microscope (explanation)
 Show a stained dry slide of onion epidermis

2 Practical session- How to use the compound light microscope (extra laboratory experiment 2) 2

 Practical quiz
 Preparation of a slide showing stomta taken from a green leaf
 Preparation of wet mount from onion epidermis
 Staining slide of onion epidermis
 how to use and take care of microscope

3 Organic Compounds (Laboratory Experiment 3 ) 2

 Carbohydrates
 Lipids
 Proteins
 Nucleic acid
 Testing for organic compounds
- Testing for Reducing sugars
- Testing for starch
- Testing for proteins
- Testing for lipids

4 Diffusion, Osmosis and Biological Membranes (Laboratory Experiment 4 ) 2

 Introduction to conceptual basics of membrane properties


 The effect of temperature on diffusion
 The effect of molecular weight on diffusion
 Membrane permeability
 Osmosis analysis
5 Limitations on Cell Size – Surface Area-to-Volume Ratio (Laboratory Experiment 5) 2

 The effect of increasing volume on cell activity


 The surface area-to-volume ration and permeability

6 Photosynthesis (Laboratory Experiment 6) 2

 Identification of starch in green leaves


 Measurement of chlorophyll absorbance under different wavelengths
 Drawing the absorbance spectrum of chlorophyll
 Spectra graphic analysis

Midterm
1 hour

7 Separation of Plant Pigments – Thin Layer Chromatography (TLC) (Laboratory Experiment 2


7)

 Chromatography basics and analysis


 Calculation of retention factor “Rf” values for each pigment

8& 9 Enzymes (Laboratory Experiments 8&9) 4

 Catechole oxidase analysis


 Formation and detection of benzoquinone
 Effect of pH on enzyme activity
 Effect of substrate concentration on enzyme activity
 The need for cofactors in enzyme activity
 Effect of temperature on enzyme activity

10 Meiosis/Mitosis and Cell Division (Laboratory Experiment 10) 2

 Conceptual basics of cell division


 Timing of the phases of cell division (mitosis)

11 Electrophoresis (Laboratory Experiment 11) 2

 Agarose gel preparation


 Separation of DNA fragments
 Visualization of separated DNA under UV light

12 Hamster Dissection (Laboratory Experiment 12) 2

 The basics of hamster dissection


 Internal anatomy
 The digestive and respiratory systems
Final Exam
1 hour

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